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  1. Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolutio...

    Authors: OndÅ™ej Kaplan, Karel BezouÅ¡ka, OndÅ™ej Plíhal, Rüdiger Ettrich, Natallia Kulik, OndÅ™ej VanÄ›k, Daniel Kavan, OldÅ™ich Benada, Anna Malandra, OndÅ™ej Å veda, Alicja B Veselá, Anna Rinágelová, Kristýna Slámová, Maria Cantarella, Jürgen Felsberg, Jarmila DuÅ¡ková…
    Citation: BMC Biotechnology 2011 11:2
    Content type: Research article Published on:

    The Retraction Note to this article has been published in BMC Biotechnology 2013 13:836

  2. The rat hybridoma cell line YB2/0 appears a good candidate for the large-scale production of low fucose recombinant mAbs due to its lower expression of fut8 gene than other commonly used rodent cell lines. Howeve...

    Authors: Béatrice Teylaert, Edwige Meurice, Marie Bobowski, Anne Harduin-Lepers, Christine Gaucher, Alexandre Fontayne, Sylvie Jorieux and Philippe Delannoy
    Citation: BMC Biotechnology 2011 11:1
    Content type: Research article Published on:

  3. Adenovirus serotype 5 (Ad5) has many favourable characteristics for development as a gene therapy vector. However, the utility of current Ad5 vectors is limited by transient transgene expression, toxicity and ...

    Authors: Susan J Morris, Daniel C Farley and Keith N Leppard
    Citation: BMC Biotechnology 2010 10:92
    Content type: Research article Published on:

  4. Monoclonal antibodies have been employed as targeting molecules of superantigen for the preclinical treatment of a variety of tumours. However, other targeting molecules, such as tumour-related ligands or pept...

    Authors: Quanbin Xu, Xiaojuan Zhang, Junjie Yue, Chuanxuan Liu, Cheng Cao, Hui Zhong and Qingjun Ma
    Citation: BMC Biotechnology 2010 10:91
    Content type: Research article Published on:

  5. Following publication of this article [1] the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science"). The correct probe that was used for the 1lox/2lox...

    Authors: Britta Weis, Joachim Schmidt, Frank Lyko and Heinz G Linhart
    Citation: BMC Biotechnology 2010 10:90
    Content type: Erratum Published on:

    The original article was published in BMC Biotechnology 2010 10:75

  6. RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies. Most of the current approaches for RNA quality asse...

    Authors: Elena A Takano, Thomas Mikeska, Alexander Dobrovic, David J Byrne and Stephen B Fox
    Citation: BMC Biotechnology 2010 10:89
    Content type: Methodology article Published on:

  8. The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening. Purification of recombinant protein in suffi...

    Authors: Anita M Dreyer, Jeremy Beauchamp, Hugues Matile and Gerd Pluschke
    Citation: BMC Biotechnology 2010 10:87
    Content type: Research article Published on:

  9. The amino acid derivative 3,4-dihydroxy L-phenylalanine (L-dopa) is gaining interest as a drug of choice for Parkinson's disease. Aspergillus oryzae is commonly used for L-dopa production; however, a slower growt...

    Authors: S Ali and I Haq
    Citation: BMC Biotechnology 2010 10:86
    Content type: Research article Published on:

  10. Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused...

    Authors: Yamei Liu, Victor G Stepanov, Ulrich Strych, Richard C Willson, George W Jackson and George E Fox
    Citation: BMC Biotechnology 2010 10:85
    Content type: Research article Published on:

  11. Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vac...

    Authors: Yan Li, Yunshan Ning, Yundan Wang, Dandan Peng, Yaodong Jiang, Lili Zhang, Min Long, Jun Luo and Ming Li
    Citation: BMC Biotechnology 2010 10:84
    Content type: Research article Published on:

  13. Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexit...

    Authors: Apostolos Alissandratos, Nina Baudendistel, Sabine L Flitsch, Bernhard Hauer and Peter J Halling
    Citation: BMC Biotechnology 2010 10:82
    Content type: Research article Published on:

  14. The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with...

    Authors: Rainer Loew, Niels Heinz, Mathias Hampf, Hermann Bujard and Manfred Gossen
    Citation: BMC Biotechnology 2010 10:81
    Content type: Methodology article Published on:

  15. Cells permissive to virus can become refractory to viral replication upon intracellular expression of single chain fragment variable (scFv) antibodies directed towards viral structural or regulatory proteins, ...

    Authors: Kuntida Kitidee, Sawitree Nangola, Gaëlle Gonzalez, Pierre Boulanger, Chatchai Tayapiwatana and Saw-See Hong
    Citation: BMC Biotechnology 2010 10:80
    Content type: Research article Published on:

  16. For improved uptake of oligonucleotide-based therapy, the oligonucleotides often are coupled to peptides that facilitate entry into cells. To this end, novel cell-penetrating peptides (CPPs) were designed for ...

    Authors: H Dan Lewis, Ali Husain, Robert J Donnelly, Dimitrios Barlos, Sheraz Riaz, Kalyani Ginjupalli, Adetola Shodeinde and Beverly E Barton
    Citation: BMC Biotechnology 2010 10:79
    Content type: Research article Published on:

  17. Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affini...

    Authors: Qiongming Liu, Qing Chen, Jian Wang, Ying Zhang, Ying Zhou, Cong Lin, Wei He, Fuchu He and Danke Xu
    Citation: BMC Biotechnology 2010 10:78
    Content type: Methodology article Published on:

  18. mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments in gene therapy m...

    Authors: Julio CC Lorenzi, Ana PF Trombone, Carolina D Rocha, Luciana P Almeida, Ricardo L Lousada, Thiago Malardo, Isabela C Fontoura, Renata AM Rossetti, Ana F Gembre, Aristóbolo M Silva, Celio L Silva and Arlete AM Coelho-Castelo
    Citation: BMC Biotechnology 2010 10:77
    Content type: Research article Published on:

  20. Conditional gene deletion using Cre-lox recombination is frequently used in mouse genetics; however recombination is frequently incomplete, resulting in a mixture of cells containing the functional (2lox) alle...

    Authors: Britta Weis, Joachim Schmidt, Frank Lyko and Heinz G Linhart
    Citation: BMC Biotechnology 2010 10:75
    Content type: Methodology article Published on:

  21. For more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe a rapid and simple gold nanoparticle (NP...

    Authors: Jiangqin Zhao, Shixing Tang, James Storhoff, Sudhakar Marla, Y Paul Bao, Xue Wang, Eric Y Wong, Viswanath Ragupathy, Zhiping Ye and Indira K Hewlett
    Citation: BMC Biotechnology 2010 10:74
    Content type: Methodology article Published on:

  22. Biotin is an essential enzyme cofactor that acts as a CO2 carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four e...

    Authors: Teegan A Delli-Bovi, Maroya D Spalding and Sean T Prigge
    Citation: BMC Biotechnology 2010 10:73
    Content type: Research article Published on:

  23. EGF receptor variant III (EGFRvIII) is the most common variant of the EGF receptor observed in human tumors. It results from the in frame deletion of exons 2-7 and the generation of a novel glycine residue at ...

    Authors: Puja Gupta, Shuang-Yin Han, Marina Holgado-Madruga, Siddhartha S Mitra, Gordon Li, Ryan T Nitta and Albert J Wong
    Citation: BMC Biotechnology 2010 10:72
    Content type: Research article Published on:

  24. The microarray has contributed to developing the omic analysis. However, as it depends basically on the surface reaction, it is hard to perform bulk reactions and sequential multistep reactions. On the other h...

    Authors: Yasunori Kinoshita, Takahiro Tayama, Koichiro Kitamura, Md Salimullah, Hidekazu Uchida, Miho Suzuki, Yuzuru Husimi and Koichi Nishigaki
    Citation: BMC Biotechnology 2010 10:71
    Content type: Methodology article Published on:

  25. Serum-containing medium (SCM), which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM) for the...

    Authors: Min Kyoung Jeon, Jong-Baeck Lim and Gyun Min Lee
    Citation: BMC Biotechnology 2010 10:70
    Content type: Research article Published on:

  26. Recent successes in biotechnological application of birds are based on their unique physiological traits such as unlimited manipulability onto developing embryos and simple protein constituents of the eggs. Ho...

    Authors: Hee W Seo, Tae M Kim, Jin W Choi, Beom K Han, Gwonhwa Song and Jae Y Han
    Citation: BMC Biotechnology 2010 10:69
    Content type: Research article Published on:

  27. Anticarsia gemmatalis is a pest in South America's soybean crops, which could be controlled by the Multinucleopolyhedrovirus of A. gemmatalis (AgMNPV). Currently, its commercial production is based on infected la...

    Authors: Diego L Mengual Gómez, Mariano N Belaich, Vanina A Rodríguez and Pablo D Ghiringhelli
    Citation: BMC Biotechnology 2010 10:68
    Content type: Research article Published on:

  29. Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In t...

    Authors: April L Ellis, Wensheng Pan, Guang Yang, Kim Jones, Christine Chuang, John M Whitelock and Arthur A DeCarlo
    Citation: BMC Biotechnology 2010 10:66
    Content type: Research article Published on:

  30. Post-operative peritoneal adhesions are common and serious complications for modern medicine. We aim to prevent post-surgical adhesions using biodegradable and thermosensitive poly(ethylene glycol)-poly(ε-capr...

    Authors: Bing Yang, ChangYang Gong, ZhiYong Qian, Xia Zhao, ZhengYu Li, XiaoRong Qi, ShengTao Zhou, Qian Zhong, Feng Luo and YuQuan Wei
    Citation: BMC Biotechnology 2010 10:65
    Content type: Research article Published on:

  31. MicroRNAs(miRNAs) are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs were discovered by conventional cloning and a Sanger sequencing approach. The next-ge...

    Authors: Geng Tian, XuYang Yin, Hong Luo, XiaoHong Xu, Lars Bolund and XiuQing Zhang
    Citation: BMC Biotechnology 2010 10:64
    Content type: Research article Published on:

  32. The D-galacturonic acid derived from plant pectin can be converted into a variety of other chemicals which have potential use as chelators, clarifiers, preservatives and plastic precursors. Among these is the ...

    Authors: Marilyn G Wiebe, Dominik Mojzita, Satu Hilditch, Laura Ruohonen and Merja Penttilä
    Citation: BMC Biotechnology 2010 10:63
    Content type: Research article Published on:

  33. Mucin type O-glycosylation is one of the most common types of post-translational modifications that impacts stability and biological functions of many mammalian proteins. A large family of UDP-GalNAc polypepti...

    Authors: Sasha M Daskalova, Josiah E Radder, Zbigniew A Cichacz, Sam H Olsen, George Tsaprailis, Hugh Mason and Linda C Lopez
    Citation: BMC Biotechnology 2010 10:62
    Content type: Research article Published on:

  36. Due to their unique ability to bind their targets with high fidelity, antibodies are used widely not only in biomedical research, but also in many clinical applications. Recombinant antibodies, including singl...

    Authors: Ole Vielemeyer, Clément Nizak, Ana Joaquina Jimenez, Arnaud Echard, Bruno Goud, Jacques Camonis, Jean-Christophe Rain and Franck Perez
    Citation: BMC Biotechnology 2010 10:59
    Content type: Research article Published on:

  37. Tissue specific promoters may be utilized for a variety of applications, including programmed gene expression in cell types, tissues and organs of interest, for developing different cell culture models or for ...

    Authors: Denis Kuzmin, Elena Gogvadze, Roman Kholodenko, Dawid P Grzela, Maxim Mityaev, Tatyana Vinogradova, Eugene Kopantzev, Galina Malakhova, Maria Suntsova, Dmitry Sokov, Zoltán Ivics and Anton Buzdin
    Citation: BMC Biotechnology 2010 10:58
    Content type: Research article Published on:

  38. Protein instability remains the main factor limiting the development of protein therapeutics. The fragile nature (structurally and chemically) of proteins makes them susceptible to detrimental events during pr...

    Authors: Giselle M Flores-Fernández, Miraida Pagán, Mariangely Almenas, Ricardo J Solá and Kai Griebenow
    Citation: BMC Biotechnology 2010 10:57
    Content type: Research article Published on:

  39. Molecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. We have established an automated, fast and flexible...

    Authors: Natacha Olieric, Melanie Kuchen, Sandro Wagen, Marion Sauter, Stephanie Crone, Sonia Edmondson, Daniel Frey, Christian Ostermeier, Michel O Steinmetz and Rolf Jaussi
    Citation: BMC Biotechnology 2010 10:56
    Content type: Methodology article Published on:

  40. The modular approach to analysis of genetically modified organisms (GMOs) relies on the independence of the modules combined (i.e. DNA extraction and GM quantification). The validity of this assumption has to ...

    Authors: Gianni Bellocchi, Marzia De Giacomo, Nicoletta Foti, Marco Mazzara, Eleonora Palmaccio, Cristian Savini, Chiara Di Domenicantonio, Roberta Onori and Guy Van den Eede
    Citation: BMC Biotechnology 2010 10:55
    Content type: Research article Published on:

  41. Recombinant gas vesicles (r-GV) from Halobacterium sp. strain SD109 expressing cassettes with different SIVsm inserts, have potential utility as an effective antigen display system for immunogen testing in vivo a...

    Authors: Marinko Sremac and Elizabeth S Stuart
    Citation: BMC Biotechnology 2010 10:54
    Content type: Research article Published on:

  42. The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transform...

    Authors: Mohamed Faize, Lydia Faize and Lorenzo Burgos
    Citation: BMC Biotechnology 2010 10:53
    Content type: Methodology article Published on:

  43. Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized a...

    Authors: Melina Laguía-Becher, Valentina Martín, Mauricio Kraemer, Mariana Corigliano, María L Yacono, Alejandra Goldman and Marina Clemente
    Citation: BMC Biotechnology 2010 10:52
    Content type: Research article Published on:

  44. Biopolymers have various applications in medicine, food and petroleum industries. The ascomycetous fungus Ophiocordyceps dipterigena BCC 2073 produces an exobiopolymer, a (1→3)-β-D-glucan, in low quantity under s...

    Authors: Kanokarn Kocharin, Pranee Rachathewee, Jean-Jacques Sanglier and Wai Prathumpai
    Citation: BMC Biotechnology 2010 10:51
    Content type: Research article Published on:

  45. The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protei...

    Authors: Yoshifumi Hashimoto, Sheng Zhang and Gary W Blissard
    Citation: BMC Biotechnology 2010 10:50
    Content type: Methodology article Published on:

    The Erratum to this article has been published in BMC Biotechnology 2012 12:12

  46. Substituted catechols are important precursors for large-scale synthesis of pharmaceuticals and other industrial products. Most of the reported chemical synthesis methods are expensive and insufficient at indu...

    Authors: Dhan Prakash, Janmejay Pandey, Bhupendra N Tiwary and Rakesh K Jain
    Citation: BMC Biotechnology 2010 10:49
    Content type: Research article Published on:

  47. Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only repli...

    Authors: Iris Delrue, Hanne Van Gorp, Jan Van Doorsselaere, Peter L Delputte and Hans J Nauwynck
    Citation: BMC Biotechnology 2010 10:48
    Content type: Research article Published on:

  48. MicroRNAs (miRs) are non-coding RNA molecules involved in post-transcriptional regulation, with diverse functions in tissue development, differentiation, cell proliferation and apoptosis. miRs may be less pron...

    Authors: Rashmi S Goswami, Levi Waldron, Jerry Machado, Nilva K Cervigne, Wei Xu, Patricia P Reis, Denis J Bailey, Igor Jurisica, Michael R Crump and Suzanne Kamel-Reid
    Citation: BMC Biotechnology 2010 10:47
    Content type: Methodology article Published on:

  49. The Host Cell Reactivation Assay (HCRA) is widely used to identify circumstances and substances affecting the repair capacity of cells, however, it is restricted by the transfection procedure used and the sens...

    Authors: Katharina Burger, Katja Matt, Nicole Kieser, Daniel Gebhard and Jörg Bergemann
    Citation: BMC Biotechnology 2010 10:46
    Content type: Methodology article Published on:

  50. To date, the detection of live microorganisms present in the environment or involved in infections is carried out by enumeration of colony forming units on agar plates, which is time consuming, laborious and l...

    Authors: Remco Kort, Andreas Nocker, Alie de Kat Angelino-Bart, Sjaak van Veen, Herman Verheij, Frank Schuren and Roy Montijn
    Citation: BMC Biotechnology 2010 10:45
    Content type: Methodology article Published on:
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