BMC Biotechnology

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Creaming behavior prediction of argan oil in water emulsion stabilized by lacto-fermentation: creaming index

Authors: Soumaya El Bouchikhi, Philippe Pagès, Azeddine Ibrahimi and Yahya Bensouda

Content type: Research article

18 September 2021
Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

Authors: So-Yun Lee, Munkhzaya Byambaragchaa, Seung-Hee Choi, Han-Ju Kang, Myung-Hwa Kang and Kwan-Sik Min

Content type: Research

5 September 2021
Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Authors: Garshasb Rigi, Amin Rostami, Habib Ghomi, Gholamreza Ahmadian, Vasiqe Sadat Mirbagheri, Meisam Jeiranikhameneh, Majid Vahed and Sahel Rahimi

Content type: Research

16 August 2021
Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Authors: Eunmi Ban, Haejin Kwon, Hong Seog Seo, Young Sook Yoo and Eun Joo Song

Content type: Methodology article

16 August 2021
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Authors: Yan Guangbo, Shu Min, Shen Wei, Ma Lixin, Zhai Chao, Wang Yaping and Huang Zunxi

Content type: Research

9 August 2021

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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Authors: Huanting Liu and James H Naismith

Content type: Methodology article

4 December 2008
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Authors: Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs and Alex F Markham

Content type: Methodology article

13 October 2003
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Authors: Oded Edelheit, Aaron Hanukoglu and Israel Hanukoglu

Content type: Methodology article

30 June 2009
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Authors: Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood and Timothy J Mitchison

Content type: Methodology article

9 September 2004
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

Authors: Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang and Yongchang Chang

Content type: Methodology article

12 October 2011

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Genome editing technologies and applications

BMC Biotechnology and BMC Plant Biology together present the latest collection of articles on the advances in genome editing technologies and applications.

Advances in CAR-T immunotherapy

BMC Biotechnology and BMC Cancer together present the latest collection of articles on the advances in CAR-T immunotherapy covering the entire range of advances from lab to clinic.

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Healthcare professionals engaging with authors

September: Patient and public involvement in research

In recognition of World Patient Safety Awareness day on 17th September, the BMC Series presents a focus issue on Patient and Public Involvement (PPI) in research. We have collated free-to-read content from across our journals - showcasing patient perspectives, research into the study of PPI, and engaging public partners as co-authors in research.

August: Breastfeeding

In celebration of World Breastfeeding week in the beginning of August, the BMC Series presents a focus issue on Breastfeeding. We have collated open-access content from across our journals to highlight research into breastfeeding behaviors and care for lactating women, infant and child health, biology of breastfeeding, and more.

Lentiviral system for genome editing

In this article the authors demonstrate a new lentivirus-driven method for genome editing of Salmon cells. Read the full article here.

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BMC Biotechnology

Articles

Creaming behavior prediction of argan oil in water emulsion stabilized by lacto-fermentation: creaming index

Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method