BMC Biotechnology

Articles

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Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids

Authors: Dilara Sen, Alexis Voulgaropoulos and Albert J. Keung

Content type: Research article

12 October 2021
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol

Authors: Christian Rapp, Simone Pival-Marko, Erika Tassano, Bernd Nidetzky and Regina Kratzer

Content type: Research article

11 October 2021
Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis

Authors: Tao Li, Chenyi Zhan, Gege Guo, Zhaoxing Liu, Ning Hao and Pingkai Ouyang

Content type: Research

7 October 2021
Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

Authors: Jianan Liu, Ke Zhang, Jie Song, Hongyan Wu, Hongshun Hao, Jingran Bi, Hongman Hou and Gongliang Zhang

Content type: Research

29 September 2021
Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Authors: Zhiwei Zhang, Yang Liu, Jing Zhao, Wenqiang Li, Ruiwen Hu, Xia Li, Aitao Li, Yaping Wang and Lixin Ma

Content type: Research article

25 September 2021

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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Authors: Huanting Liu and James H Naismith

Content type: Methodology article

4 December 2008
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Authors: Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs and Alex F Markham

Content type: Methodology article

13 October 2003
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Authors: Oded Edelheit, Aaron Hanukoglu and Israel Hanukoglu

Content type: Methodology article

30 June 2009
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Authors: Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood and Timothy J Mitchison

Content type: Methodology article

9 September 2004
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

Authors: Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang and Yongchang Chang

Content type: Methodology article

12 October 2011

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Valuable data often go unpublished when they could be helping to progress science. Hence, the BMC Series introduced Data notes, a short article type allowing you to describe your data and publish them to make your data easier to find, cite and share.

You can publish your data in BMC Genomic Data (genomic, transcriptomic and high-throughput genotype data) or in BMC Research Notes (data from across all natural and clinical sciences).

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New Collections

Genome editing technologies and applications

BMC Biotechnology and BMC Plant Biology together present the latest collection of articles on the advances in genome editing technologies and applications.

Advances in CAR-T immunotherapy

BMC Biotechnology and BMC Cancer together present the latest collection of articles on the advances in CAR-T immunotherapy covering the entire range of advances from lab to clinic.

BMC Series Focus Issues

October: Focus on Stem Cells

With Stem Cell Awareness day on the 13th October this year we have compiled an article collection from across different BMC Series journals covering aspects of basic stem cell biology; in vitro differentiation studies; stem-cell based therapy studies for various conditions in animal models; technical advancements and ethical issues.

Healthcare professionals engaging with authors

September: Patient and public involvement in research

In recognition of World Patient Safety Awareness day on 17th September, the BMC Series presents a focus issue on Patient and Public Involvement (PPI) in research. We have collated free-to-read content from across our journals - showcasing patient perspectives, research into the study of PPI, and engaging public partners as co-authors in research.

Lentiviral system for genome editing

In this article the authors demonstrate a new lentivirus-driven method for genome editing of Salmon cells. Read the full article here.

Aims and scope

BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.

The journal welcomes submissions in these broad areas of research:
Applied immunology
Cellular, tissue and whole organism biotechnology
Drug discovery and delivery
Environmental biotechnology
Food and nutritional biotechnology
Genetic engineering
Mathematical modeling and statistics in biology
Methods and technological advances in biotechnology
Microbial biotechnology
Plant and agricultural biotechnology
Systems and synthetic biology

To read the full scope of the journal, click here.

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Can Kidney Disease Affect Your Thinking?

14 October 2021
Highlights of the BMC Series – September 2021

11 October 2021
Introducing the BMC Series SDG Editorial Board Members: Harvy Joy Liwanag

30 September 2021

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BMC Biotechnology

Articles

Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids

Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol

Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method