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  1. Methodology article

    Overview of methodologies for T-cell receptor repertoire analysis

    The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

    Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

    BMC Biotechnology 2017 17:61

    Published on: 10 July 2017

  2. Research article

    Unusual acylation of chloramphenicol in Lysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics

    The environmental gliding bacteria Lysobacter are emerging as a new group of biocontrol agents due to their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsi...

    Wei Zhang, Justin Huffman, Shengying Li, Yuemao Shen and Liangcheng Du

    BMC Biotechnology 2017 17:59

    Published on: 4 July 2017

  3. Research article

    Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli

    Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid ...

    Karim E. Jaén, Juan-Carlos Sigala, Roberto Olivares-Hernández, Karsten Niehaus and Alvaro R. Lara

    BMC Biotechnology 2017 17:60

    Published on: 4 July 2017

  4. Research Article

    Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    BMC Biotechnology 2017 17:55

    Published on: 21 June 2017

  5. Methodology article

    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    BMC Biotechnology 2017 17:54

    Published on: 20 June 2017

  6. Research article

    Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    BMC Biotechnology 2017 17:52

    Published on: 12 June 2017

  7. Research article

    Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    BMC Biotechnology 2017 17:49

    Published on: 6 June 2017

  8. Research article

    Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by...

    Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    BMC Biotechnology 2017 17:48

    Published on: 2 June 2017

  9. Research article

    Biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from Geobacillus thermodenitrificans T12

    Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is pre...

    Martinus J.A. Daas, Patricia Murciano Martínez, Antonius H.P. van de Weijer, John van der Oost, Willem M. de Vos, Mirjam A. Kabel and Richard van Kranenburg

    BMC Biotechnology 2017 17:44

    Published on: 18 May 2017

  10. Methodology article

    AAV vector distribution in the mouse respiratory tract following four different methods of administration

    Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these metho...

    Lisa A. Santry, Joelle C. Ingrao, Darrick L. Yu, Jondavid G. de Jong, Laura P. van Lieshout, Geoffrey A. Wood and Sarah K. Wootton

    BMC Biotechnology 2017 17:43

    Published on: 15 May 2017

  11. Research article

    Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    BMC Biotechnology 2017 17:42

    Published on: 12 May 2017

  12. Research article

    Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    BMC Biotechnology 2017 17:40

    Published on: 2 May 2017

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