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  1. Content type: Methodology article

    The CRISPR/Cas9 system has been widely used to generate gene knockout/knockin models by inducing frameshift mutants in cell lines and organisms. Several recent studies have reported that such mutants can lead ...

    Authors: Dafeng Chen, Ji-Xin Tang, Beibei Li, Li Hou, Xianhui Wang and Le Kang

    Citation: BMC Biotechnology 2018 18:60

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  2. Content type: Research article

    Transgenic technology has become an important technique for crop genetic improvement. The application of well-characterized promoters is essential for developing a vector system for efficient genetic transform...

    Authors: Pingping Jiang, Ke Zhang, Zhaohua Ding, Qiuxia He, Wendi Li, Shuangfeng Zhu, Wen Cheng, Kewei Zhang and Kunpeng Li

    Citation: BMC Biotechnology 2018 18:59

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  3. Content type: Research article

    Microbial flocculation is a good choice for harvest of microalgae biomass, which has gained extensive attention. There have been carried out massive studies in bacterial flocculation, many bacterial strains wi...

    Authors: Yi Li, Yanting Xu, Ruixue Song, Congqi Tian, Lei Liu, Tianling Zheng and Hailei Wang

    Citation: BMC Biotechnology 2018 18:58

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  4. Content type: Research article

    Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties. However, industrial applications of LO...

    Authors: Hui Qian, Chong Zhang, Zhaoxin Lu, Bingjie Xia, Xiaomei Bie, Haizhen Zhao, Fengxia Lu and Guang-Yu Yang

    Citation: BMC Biotechnology 2018 18:57

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  5. Content type: Methodology article

    Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this ...

    Authors: Hirotatsu Ohkawara, Shigeru Miyagawa, Satsuki Fukushima, Shin Yajima, Atsuhiro Saito, Hiroshi Nagashima and Yoshiki Sawa

    Citation: BMC Biotechnology 2018 18:56

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  6. Content type: Research article

    Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used to produce ant...

    Authors: Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge and C. Ronald Geyer

    Citation: BMC Biotechnology 2018 18:55

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  7. Content type: Research article

    Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell...

    Authors: Guotian Li, Kyle C. Jones, Aymerick Eudes, Venkataramana R. Pidatala, Jian Sun, Feng Xu, Chengcheng Zhang, Tong Wei, Rashmi Jain, Devon Birdseye, Patrick E. Canlas, Edward E. K. Baidoo, Phat Q. Duong, Manoj K. Sharma, Seema Singh, Deling Ruan…

    Citation: BMC Biotechnology 2018 18:54

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  8. Content type: Research article

    Cyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical...

    Authors: Yujia Jiang, Jie Zhou, Ruofan Wu, Fengxue Xin, Wenming Zhang, Yan Fang, Jiangfeng Ma, Weiliang Dong and Min Jiang

    Citation: BMC Biotechnology 2018 18:53

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  9. Content type: Research Article

    Fibroblast growth factor 9 (FGF9) is a heparin-binding growth factor, secreted by both mesothelial and epithelial cells, which participates in hair follicle regeneration, wound healing, and bone development. A...

    Authors: Jingbo Cai, Ruicheng Wen, Wenqing Li, Xiuran Wang, Haishan Tian, Shanyong Yi, Linbo Zhang, Xiaokun Li, Chao Jiang and Haiyan Li

    Citation: BMC Biotechnology 2018 18:51

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  10. Content type: Research article

    Arthropod-borne diseases remain a leading cause of human morbidity and mortality and exact an enormous toll on global agriculture. The practice of insecticide-based control is fraught with issues of excessive ...

    Authors: Arinder K. Arora, Kendra N. Pesko, Verónica Quintero-Hernández, Lourival D. Possani, Thomas A. Miller and Ravi V. Durvasula

    Citation: BMC Biotechnology 2018 18:50

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  11. Content type: Research article

    Integration of first- and second-generation ethanol processes is one among the alternate approaches that efficiently address the current socio-economic issues of the bioethanol sector. Edible filamentous fungu...

    Authors: Ramkumar B. Nair, Osagie A. Osadolor, Vamsi K. Ravula, Patrik R. Lennartsson and Mohammad J. Taherzadeh

    Citation: BMC Biotechnology 2018 18:49

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  12. Content type: Research article

    Ricin A chain (RTA) and Pokeweed antiviral proteins (PAPs) are plant-derived N-glycosidase ribosomal-inactivating proteins (RIPs) isolated from Ricinus communis and Phytolacca Americana respectively. This study w...

    Authors: Yasser Hassan, Sherry Ogg and Hui Ge

    Citation: BMC Biotechnology 2018 18:47

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  13. Content type: Research article

    The bacterial nicotine-degrading enzyme NicA2 isolated from P. putida was studied to assess its potential use in the treatment of tobacco dependence.

    Authors: Paul R. Pentel, Michael D. Raleigh, Mark G. LeSage, Thomas Thisted, Stephen Horrigan, Zuzana Biesova and Matthew W. Kalnik

    Citation: BMC Biotechnology 2018 18:46

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  14. Content type: Review

    Genome editing technologies offers new opportunities for tackling diseases such as acute lymphoblastic leukemia (ALL) that have been beyond the reach of previous therapies.

    Authors: Adrián Montaño, Maribel Forero-Castro, Jesús-María Hernández-Rivas, Ignacio García-Tuñón and Rocío Benito

    Citation: BMC Biotechnology 2018 18:45

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  15. Content type: Methodology article

    With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient c...

    Authors: Qin Liu, Hui-Jie Dang, Yuan-Hang Wu, Min Li, Yin-Hua Chen, Xiao-Lei Niu, Kai-Mian Li and Li-Juan Luo

    Citation: BMC Biotechnology 2018 18:44

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  16. Content type: Research article

    Cellulose is the most important component of lignocellulose, and its degradation requires three different types of enzymes to act synergistically. There have been reports of single gene duality, but no gene ha...

    Authors: Zi-Lu Liu, Hua-Nan Li, Hui-Ting Song, Wen-Jing Xiao, Wu-Cheng Xia, Xiao-Peng Liu and Zheng-Bing Jiang

    Citation: BMC Biotechnology 2018 18:43

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  17. Content type: Research article

    Consolidated bioprocessing (CBP) is a cost-effective approach for the conversion of lignocellulosic biomass to biofuels and biochemicals. The enzymatic conversion of cellulose to glucose requires the synergist...

    Authors: Martinus J. A. Daas, Bart Nijsse, Antonius H. P. van de Weijer, Bart W. A. J. Groenendaal, Fons Janssen, John van der Oost and Richard van Kranenburg

    Citation: BMC Biotechnology 2018 18:42

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  18. Content type: Research article

    Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural assoc...

    Authors: Marie-Alice Fraiture, Julie Vandamme, Philippe Herman and Nancy H. C. Roosens

    Citation: BMC Biotechnology 2018 18:40

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  19. Content type: Methodology article

    Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early i...

    Authors: Rashmi Sharma, Fetweh H. Al-Saleem, Rama Devudu Puligedda, Amy Rattelle, David R. Lynch and Scott K. Dessain

    Citation: BMC Biotechnology 2018 18:41

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  20. Content type: Research article

    Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH)...

    Authors: Yasuhiro Watanabe, Takashi Matsuba, Mami Nakanishi, Mio Une, Ritsuko Hanajima and Kenji Nakashima

    Citation: BMC Biotechnology 2018 18:39

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  21. Content type: Methodology article

    Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. Th...

    Authors: Yuliya Khilko, Philip D. Weyman, John I. Glass, Mark D. Adams, Melanie A. McNeil and Peter B. Griffin

    Citation: BMC Biotechnology 2018 18:37

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  22. Content type: Research article

    Cellulases are of great significance for full utilization of lignocellulosic biomass. Termites have an efficient ability to degrade cellulose. Heterologous production of the termite-origin cellulases is the fi...

    Authors: Pengfei Zhang, Xianghua Yuan, Yuguang Du and Jian-Jun Li

    Citation: BMC Biotechnology 2018 18:35

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  23. Content type: Research article

    The advent of –omics technologies has enabled the resolution of fine molecular differences among individuals within a species. DNA sequence variations, such as single nucleotide polymorphisms or small deletion...

    Authors: Jean-Michel Michno and Robert M. Stupar

    Citation: BMC Biotechnology 2018 18:38

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  24. Content type: Research article

    Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to...

    Authors: Nanyu Han, Huabiao Miao, Tingting Yu, Bo Xu, Yunjuan Yang, Qian Wu, Rui Zhang and Zunxi Huang

    Citation: BMC Biotechnology 2018 18:36

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  25. Content type: Research article

    High level of serum fibroblast growth factor 23 (FGF23) is implicated in the development and progression of diabetic nephropathy (DN), making it a crucial factor in the pathogenesis of DN. FGF23 is also tightl...

    Authors: Xiaomin Zhang, Kaiwen Guo, Feng Xia, Xinyu Zhao, Zhifeng Huang and Jianlou Niu

    Citation: BMC Biotechnology 2018 18:33

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  26. Content type: Research article

    Celiac disease (CD) is one of the most common food-related chronic disorders. It is mediated by the dietary consumption of prolamins, which are storage proteins of different grains. So far, no therapy exists a...

    Authors: Britta Eggenreich, Elke Scholz, David Johannes Wurm, Florian Forster and Oliver Spadiut

    Citation: BMC Biotechnology 2018 18:30

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  27. Content type: Methodology article

    According to recent studies, the phospholipase A2 receptor 1 (PLA2R1) may be used as a biomarker to diagnose idiopathic membranous nephropathy (iMN). Moreover, the immune-dominant regions of PLA2R1 have been iden...

    Authors: Hua Li, Yonghua Xu, Weiming Yang and Xiaohong Huang

    Citation: BMC Biotechnology 2018 18:31

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  28. Content type: Research article

    Solvate ionic liquids (SILs) are a new class of ionic liquids that are equimolar solutions of lithium bistrifluoromethanesulfonimide in either triglyme or tetraglyme, referred to as G3LiTFSA and G4LiTFSA, resp...

    Authors: Prusothman Yoganantharajah, Alexander P. Ray, Daniel J. Eyckens, Luke C. Henderson and Yann Gibert

    Citation: BMC Biotechnology 2018 18:32

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  29. Content type: Research article

    β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides to xylose and to biotransform some saponins by cleaving outer β-xylose. β-D-xylosidase is widely used as one of the x...

    Authors: Qi Li, Tao Wu, Zhipeng Qi, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:29

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  30. Content type: Research article

    Nonsense mutations promote premature translational termination, introducing stop codons within the coding region of mRNAs and causing inherited diseases, including thalassemia. For instance, in β039 thalassemia t...

    Authors: Francesca Salvatori, Mariangela Pappadà, Giulia Breveglieri, Elisabetta D’Aversa, Alessia Finotti, Ilaria Lampronti, Roberto Gambari and Monica Borgatti

    Citation: BMC Biotechnology 2018 18:28

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  31. Content type: Research article

    As a very important coenzyme in the cell metabolism, Vitamin B12 (cobalamin, VB12) has been widely used in food and medicine fields. The complete biosynthesis of VB12 requires approximately 30 genes, but overexpr...

    Authors: Yingying Cai, Miaomiao Xia, Huina Dong, Yuan Qian, Tongcun Zhang, Beiwei Zhu, Jinchuan Wu and Dawei Zhang

    Citation: BMC Biotechnology 2018 18:27

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  32. Content type: Research article

    Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restr...

    Authors: Xuesong Zhang, Shiyong Liao, Fuliang Cao, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:26

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  33. Content type: Research article

    Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive ...

    Authors: Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang and Kunzhi Jia

    Citation: BMC Biotechnology 2018 18:25

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  34. Content type: Research article

    Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the be...

    Authors: Huidan Zhang, Dong Lu, Xin Li, Yingang Feng, Qiu Cui and Xiaojin Song

    Citation: BMC Biotechnology 2018 18:23

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  35. Content type: Research article

    The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and in...

    Authors: Xiaoyue Zhang, Keyan Xu, Yanmei Ou, Xiaodong Xu and Hongying Chen

    Citation: BMC Biotechnology 2018 18:24

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  36. Content type: Research article

    Violacein is a deep violet compound that is produced by a number of bacterial species. It is synthesized from tryptophan by a pathway that involves the sequential action of 5 different enzymes (encoded by genes v...

    Authors: Elizabeth Bilsland, Tatyana A. Tavella, Renata Krogh, Jamie E. Stokes, Annabelle Roberts, James Ajioka, David R. Spring, Adriano D. Andricopulo, Fabio T. M. Costa and Stephen G. Oliver

    Citation: BMC Biotechnology 2018 18:22

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  37. Content type: Research article

    Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin’s N-terminus must engage thrombin’s active site for effective inhibition, fusing a ...

    Authors: William P. Sheffield, Louise J. Eltringham-Smith and Varsha Bhakta

    Citation: BMC Biotechnology 2018 18:21

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  38. Content type: Methodology article

    Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these...

    Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero and Marco A. Ramos

    Citation: BMC Biotechnology 2018 18:20

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  39. Content type: Research article

    Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal...

    Authors: Tomoe Kobayashi, Masumi Namba, Takayuki Koyano, Masaki Fukushima, Masahiro Sato, Masato Ohtsuka and Makoto Matsuyama

    Citation: BMC Biotechnology 2018 18:19

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  40. Content type: Research article

    Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high...

    Authors: Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa and Chiara Schiraldi

    Citation: BMC Biotechnology 2018 18:18

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  41. Content type: Methodology article

    We demonstrate the feasibility of creating a pair of reference samples to be used as surrogates for clinical samples measured in either a research or clinical laboratory setting. The reference sample paradigm ...

    Authors: P. Scott Pine, Steven P. Lund, Sanford A. Stass, Debra Kukuruga, Feng Jiang, Lynn Sorbara, Sudhir Srivastava and Marc Salit

    Citation: BMC Biotechnology 2018 18:17

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  42. Content type: Research article

    microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, ...

    Authors: Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson and Peter J. Leedman

    Citation: BMC Biotechnology 2018 18:16

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  43. Content type: Research article

    Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). Proof of concept studies have demonstrate...

    Authors: Christian A. Schaer, Catherine Owczarek, Jeremy W. Deuel, Stefan Schauer, Jin Hyen Baek, Ayla Yalamanoglu, Matthew P. Hardy, Pierre D. Scotney, Peter M. Schmidt, Matthias Pelzing, Peter Soupourmas, Paul W. Buehler and Dominik J. Schaer

    Citation: BMC Biotechnology 2018 18:15

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  44. Content type: Research article

    Dedifferentiation of chondrocytes during cell expansion is one of the barriers in tissue construction for cartilage repair. To understand chondrocyte behavior and improve cell expansion in monolayer culture, t...

    Authors: Sopita Wongin, Saranatra Waikakul, Pojchong Chotiyarnwong, Wanwipa Siriwatwechakul, Masahiro Kino-oka, Mee-Hae Kim and Kwanchanok Viravaidya-Pasuwat

    Citation: BMC Biotechnology 2018 18:14

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  45. Content type: Research article

    Filamentous bacteria of the genus Streptomyces produce a large arsenal of industrially relevant antibiotics and enzymes. The industrial production of these molecules occurs in large fermenters, where many strepto...

    Authors: Boris Zacchetti, Agathoklis Andrianos, Dino van Dissel, Evelien de Ruiter, Gilles P. van Wezel and Dennis Claessen

    Citation: BMC Biotechnology 2018 18:13

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  46. Content type: Research article

    Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding f...

    Authors: Shasha Yang, Sili Wei, Yun Mao, Hanxue Zheng, Juantao Feng, Jihong Cui, Xin Xie, Fulin Chen and Honmgmin Li

    Citation: BMC Biotechnology 2018 18:12

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  47. Content type: Research article

    Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression o...

    Authors: Paola Mini, Olivia Costantina Demurtas, Silvia Valentini, Patrizia Pallara, Giuseppe Aprea, Paola Ferrante and Giovanni Giuliano

    Citation: BMC Biotechnology 2018 18:11

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