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  1. Content type: Methodology article

    The potassium dichromate oxidation method used in determination of alcohols in fermentation has two major disadvantages. This method cannot be used to determine alcohols in raw fermentation broth samples, whic...

    Authors: Peng Zhang, Hao Hai, Dongxu Sun, Weihua Yuan, Weijie Liu, Ruru Ding, Mengting Teng, Lin Ma, Jun Tian and Caifa Chen

    Citation: BMC Biotechnology 2019 19:30

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  2. Content type: Research article

    Several types of phospholipases have been described in phospholipids modification. The majority of phospholipase D (PLD) superfamily members can catalyze two separate reactions: the hydrolysis of phospholipids...

    Authors: Haiyang Zhang, Wenqin Chu, Jianan Sun, Zhen Liu, Wen-can Huang, Changhu Xue and Xiangzhao Mao

    Citation: BMC Biotechnology 2019 19:29

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  3. Content type: Research article

    In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumum...

    Authors: Tao Yu, Chunxia Qiao, Ming Lv and Luqun Tang

    Citation: BMC Biotechnology 2019 19:28

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  4. Content type: Methodology article

    The current limitations of conventional BCG vaccines highlights the importance in developing novel and effective vaccines against tuberculosis (TB). The utilization of probiotics such as Lactobacillus plantarum f...

    Authors: Anhar Danial Mustafa, Jeevanathan Kalyanasundram, Sarah Sabidi, Adelene Ai-Lian Song, Maha Abdullah, Raha Abdul Rahim and Khatijah Yusoff

    Citation: BMC Biotechnology 2019 19:27

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  5. Content type: Research article

    Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expr...

    Authors: M. Alejandro Carballo-Amador, Edward A. McKenzie, Alan J. Dickson and Jim Warwicker

    Citation: BMC Biotechnology 2019 19:26

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  6. Content type: Research article

    The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the effic...

    Authors: Kayla Carey, Junghyun Ryu, Kyungjun Uh, Andrea J. Lengi, Sherrie Clark-Deener, Benjamin A. Corl and Kiho Lee

    Citation: BMC Biotechnology 2019 19:25

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  7. Content type: Research article

    Increasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B

    Authors: Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen and Guohao He

    Citation: BMC Biotechnology 2019 19:24

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  8. Content type: Research article

    The gene transduction efficiency of adenovirus to hematopoietic cells, especially T lymphocytes, is needed to be improved. The purpose of this study is to improve the transduction efficiency of T lymphocytes b...

    Authors: Yun Lv, Feng-Jun Xiao, Yi Wang, Xiao-Hui Zou, Hua Wang, Hai-Yan Wang, Li-Sheng Wang and Zhuo-Zhuang Lu

    Citation: BMC Biotechnology 2019 19:23

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  9. Content type: Research article

    The importance of the accessory enzymes such as α-L-arabinofuranosidases (AFases) in synergistic interactions within cellulolytic mixtures has introduced a paradigm shift in the search for hydrolytic enzymes. ...

    Authors: Brent Fortune, Sizwe Mhlongo, Leonardo Joaquim van Zyl, Robert Huddy, Mariette Smart and Marla Trindade

    Citation: BMC Biotechnology 2019 19:22

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  10. Content type: Research article

    A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in...

    Authors: Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, Dawn M. Kilkenny and Julie Audet

    Citation: BMC Biotechnology 2019 19:21

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  11. Content type: Methodology article

    Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable f...

    Authors: Régis P. Lemaitre, Aliona Bogdanova, Barbara Borgonovo, Jeffrey B. Woodruff and David N. Drechsel

    Citation: BMC Biotechnology 2019 19:20

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  12. Content type: Research article

    Recently, it has been indicated that anaerobic co-digestion of waste activated sludge with other waste streams at wastewater treatment plants is a promising strategy for enhancing methane production and materi...

    Authors: Seyed Mostafa Hallaji, Mohammad Kuroshkarim and Seyede Parvin Moussavi

    Citation: BMC Biotechnology 2019 19:19

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  13. Content type: Methodology article

    The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editi...

    Authors: Soumaya Najah, Corinne Saulnier, Jean-Luc Pernodet and Stéphanie Bury-Moné

    Citation: BMC Biotechnology 2019 19:18

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  14. Content type: Research article

    Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The organism has been proposed to be a suitable organism...

    Authors: Alexandria T. N. Holland, Michael J. Danson and Albert Bolhuis

    Citation: BMC Biotechnology 2019 19:17

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  15. Content type: Research article

    Norcantharidin (NCTD), a demethylated derivative of cantharidin (defensive toxin of blister beetles), has been reported to exhibit insecticidal activity against various types of agricultural pests. However, NC...

    Authors: Liya Zeng, Yongchang Liu, Jun Pan and Xiaowen Liu

    Citation: BMC Biotechnology 2019 19:16

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  16. Content type: Research article

    Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To exte...

    Authors: Guilherme Souza Prado, Pingdwende Kader Aziz Bamogo, Joel Antônio Cordeiro de Abreu, François-Xavier Gillet, Vanessa Olinto dos Santos, Maria Cristina Mattar Silva, Jean-Paul Brizard, Marcelo Porto Bemquerer, Martine Bangratz, Christophe Brugidou, Drissa Sérémé, Maria Fatima Grossi-de-Sa and Séverine Lacombe

    Citation: BMC Biotechnology 2019 19:15

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  17. Content type: Research article

    Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical...

    Authors: Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim and Kwang-Chul Kwon

    Citation: BMC Biotechnology 2019 19:13

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  18. Content type: Correction

    Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below:

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

    Citation: BMC Biotechnology 2019 19:12

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    The original article was published in BMC Biotechnology 2019 19:5

  19. Content type: Research article

    Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study ...

    Authors: Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar and Waldemar Rymowicz

    Citation: BMC Biotechnology 2019 19:11

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  20. Content type: Research article

    Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC...

    Authors: Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang and Weibing Xu

    Citation: BMC Biotechnology 2019 19:10

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  21. Content type: Research article

    Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system...

    Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang

    Citation: BMC Biotechnology 2019 19:9

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  22. Content type: Research article

    We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter...

    Authors: Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani and Vadood Razavilar

    Citation: BMC Biotechnology 2019 19:8

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  23. Content type: Research article

    Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and effici...

    Authors: Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen, Weizhi Ji and Yuyu Niu

    Citation: BMC Biotechnology 2019 19:7

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  24. Content type: Research article

    MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

    Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu

    Citation: BMC Biotechnology 2019 19:6

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  25. Content type: Research article

    Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic or...

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

    Citation: BMC Biotechnology 2019 19:5

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    The Correction to this article has been published in BMC Biotechnology 2019 19:12

  26. Content type: Research article

    Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent yea...

    Authors: Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid and Supachai Vuttipongchaikij

    Citation: BMC Biotechnology 2019 19:4

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  27. Content type: Research article

    A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterol...

    Authors: Nguyen Thanh Trung, Nguyen Minh Hung, Nguyen Huy Thuan, Nguyen Xuan Canh, Thomas Schweder and Britta Jürgen

    Citation: BMC Biotechnology 2019 19:3

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  28. Content type: Research article

    Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Rece...

    Authors: Kiyoung Eun, Min Gi Park, Yeon Woo Jeong, Yeon Ik Jeong, Sang-Hwan Hyun, Woo Suk Hwang, Sung-Hak Kim and Hyunggee Kim

    Citation: BMC Biotechnology 2019 19:1

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  29. Content type: Research article

    Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the prote...

    Authors: Pollyana Santos Queiroz, France Anne Dias Ruas, Natália Rocha Barboza, William de Castro Borges and Renata Guerra-Sá

    Citation: BMC Biotechnology 2018 18:83

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  30. Content type: Research article

    The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manuf...

    Authors: Lissa R. Herron, Clare Pridans, Matthew L. Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel J. Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David A. Hume, Andrew C. Gill and Helen M. Sang

    Citation: BMC Biotechnology 2018 18:82

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  31. Content type: Research article

    The methanol-regulated AOX1 promoter (PAOX1) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol d...

    Authors: Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong and Ching-Tsan Huang

    Citation: BMC Biotechnology 2018 18:81

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  32. Content type: Research article

    More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between differe...

    Authors: Zheng-Wei Qu, Si-Ya Zhou, Shi-Xin Guan, Rui Gao, Zuo-Wen Duan, Xin Zhang, Wei-Yan Sun, Wen-Li Fan, Shui-Sen Chen, Li-Jing Chen, Jing-Wei Lin and Yan-Ye Ruan

    Citation: BMC Biotechnology 2018 18:80

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  33. Content type: Research article

    Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complica...

    Authors: Ronald R. Navarro, Yuichiro Otsuka, Masanobu Nojiri, Shigehiro Ishizuka, Masaya Nakamura, Kazuhiro Shikinaka, Kenji Matsuo, Kei Sasaki, Ken Sasaki, Kazuhide Kimbara, Yutaka Nakashimada and Junichi Kato

    Citation: BMC Biotechnology 2018 18:79

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  34. Content type: Research article

    Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfol...

    Authors: Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro, Luís Carlos de Souza Ferreira and Ligia Morganti

    Citation: BMC Biotechnology 2018 18:78

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  35. Content type: Research article

    Rift Valley fever virus (RVFV), the causative agent of Rift Valley fever, is an enveloped single-stranded negative-sense RNA virus in the genus Phlebovirus, family Bunyaviridae. The virus is spread by infected mo...

    Authors: Sandiswa Mbewana, Ann E. Meyers, Brandon Weber, Vuyokazi Mareledwane, Maryke L. Ferreira, Phelix A. O. Majiwa and Edward P. Rybicki

    Citation: BMC Biotechnology 2018 18:77

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  36. Content type: Methodology article

    Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated b...

    Authors: Jonathan Bouvette, Dursun Nizam Korkut, Aurélien Fouillen, Soumiya Amellah, Antonio Nanci, Yves Durocher, James G. Omichinski and Pascale Legault

    Citation: BMC Biotechnology 2018 18:76

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  37. Content type: Research article

    The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiat...

    Authors: Aijia Cai, Moritz Hardt, Paul Schneider, Rafael Schmid, Claudia Lange, Dirk Dippold, Dirk W. Schubert, Anja M. Boos, Annika Weigand, Andreas Arkudas, Raymund E. Horch and Justus P. Beier

    Citation: BMC Biotechnology 2018 18:75

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  38. Content type: Research article

    This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed...

    Authors: Abebe Gebeyehu, Nurelegne Shebeshe, Helmut Kloos and Solomon Belay

    Citation: BMC Biotechnology 2018 18:74

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  39. Content type: Methodology article

    Early detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine is necessary to control this devastating disease. By monitoring host serum antibodies to viral antigens, early ...

    Authors: Jie Li, Gang Wang, Di Yang, Bao Zhao, Yongpan Zhao, Yonggang Liu, Xuehui Cai, Yuchen Nan, En-Min Zhou and Chunyan Wu

    Citation: BMC Biotechnology 2018 18:73

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  40. Content type: Research article

    Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020.

    Authors: Hesham A. El Enshasy, Elsayed Ahmed Elsayed, Noorhamizah Suhaimi, Roslinda Abd Malek and Mona Esawy

    Citation: BMC Biotechnology 2018 18:71

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  41. Content type: Research article

    Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of va...

    Authors: Santhanasabapathy Rajasekaran, Jayashree Thatte, Jayaprakash Periasamy, Alok Javali, Manjunath Jayaram, Dwaipayan Sen, Akshaya Krishnagopal, Giridhara R. Jayandharan and Ramkumar Sambasivan

    Citation: BMC Biotechnology 2018 18:70

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  42. Content type: Research article

    Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its nov...

    Authors: Bao Tang, Cheng Sun, Yancun Zhao, Huiyong Xu, Gaoge Xu and Fengquan Liu

    Citation: BMC Biotechnology 2018 18:69

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  43. Content type: Research article

    Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5.7 GB), low ploidy (2n = 2x = 14, AmAm) and high genetic polymorphism make this s...

    Authors: Dmitry Miroshnichenko, Danila Ashin, Alexander Pushin and Sergey Dolgov

    Citation: BMC Biotechnology 2018 18:68

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  44. Content type: Research article

    As the protein-laden by-product, red blood cells (RBCs) from poultry blood is a potential source of protein used as food and feed ingredient. However, RBC was currently underutilized. Therefore, it is an urgen...

    Authors: Zhaojun Zheng, Xubiao Wei, Tingting Shang, Yan Huang, Cong Hu and Rijun Zhang

    Citation: BMC Biotechnology 2018 18:67

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  45. Content type: Research article

    Targeted DNA integration is widely used in basic research and commercial applications because it eliminates positional effects on transgene expression. Targeted integration in mammalian cells is generally achi...

    Authors: Xiaofeng Liu, Min Wang, Yufeng Qin, Xuan Shi, Peiqing Cong, Yaosheng Chen and Zuyong He

    Citation: BMC Biotechnology 2018 18:66

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