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  1. Research article

    Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

    Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards t...

    Beatriz Temer, Leandro Vieira dos Santos, Victor Augusti Negri, Juliana Pimentel Galhardo, Pedro Henrique Mello Magalhães, Juliana José, Cidnei Marschalk, Thamy Lívia Ribeiro Corrêa, Marcelo Falsarella Carazzolle and Gonçalo Amarante Guimarães Pereira

    BMC Biotechnology 2017 17:71

    Published on: 9 September 2017

  2. Research article

    Effect of supercritical carbon dioxide on the enzymatic production of biodiesel from waste animal fat using immobilized Candida antarctica lipase B variant

    Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed...

    Aldricho Alpha Pollardo, Hong-shik Lee, Dohoon Lee, Sangyong Kim and Jaehoon Kim

    BMC Biotechnology 2017 17:70

    Published on: 9 September 2017

  3. Methodology article

    A bioinformatics approach for identifying transgene insertion sites using whole genome sequencing data

    Genetically modified crops (GM crops) have been developed to improve the agricultural traits of modern crop cultivars. Safety assessments of GM crops are of paramount importance in research at developmental st...

    Doori Park, Su-Hyun Park, Yong Wook Ban, Youn Shic Kim, Kyoung-Cheul Park, Nam-Soo Kim, Ju-Kon Kim and Ik-Young Choi

    BMC Biotechnology 2017 17:67

    Published on: 15 August 2017

  4. Research article

    Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor

    Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engr...

    Rebecca L. L. Moore, Matthew J. Worrallo, Peter D. Mitchell, Jon Harriman, Katie E. Glen and Robert J. Thomas

    BMC Biotechnology 2017 17:65

    Published on: 4 August 2017

  5. Research article

    Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops

    Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel C...

    Maniraj Rathinam, Shweta Singh, Debasis Pattanayak and Rohini Sreevathsa

    BMC Biotechnology 2017 17:64

    Published on: 2 August 2017

  6. Methodology article

    Overview of methodologies for T-cell receptor repertoire analysis

    The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

    Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

    BMC Biotechnology 2017 17:61

    Published on: 10 July 2017

  7. Research article

    Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli

    Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid ...

    Karim E. Jaén, Juan-Carlos Sigala, Roberto Olivares-Hernández, Karsten Niehaus and Alvaro R. Lara

    BMC Biotechnology 2017 17:60

    Published on: 4 July 2017

  8. Research article

    Unusual acylation of chloramphenicol in Lysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics

    The environmental gliding bacteria Lysobacter are emerging as a new group of biocontrol agents due to their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsi...

    Wei Zhang, Justin Huffman, Shengying Li, Yuemao Shen and Liangcheng Du

    BMC Biotechnology 2017 17:59

    Published on: 4 July 2017

  9. Research Article

    Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    BMC Biotechnology 2017 17:55

    Published on: 21 June 2017

  10. Methodology article

    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    BMC Biotechnology 2017 17:54

    Published on: 20 June 2017

  11. Research article

    Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    BMC Biotechnology 2017 17:52

    Published on: 12 June 2017

  12. Research article

    Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    BMC Biotechnology 2017 17:49

    Published on: 6 June 2017

  13. Research article

    Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by...

    Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    BMC Biotechnology 2017 17:48

    Published on: 2 June 2017

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