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  1. Content type: Research article

    S-Phenyl-L-cysteine is regarded as having potential applicability as an antiretroviral/protease inhibitor for human immunodeficiency virus (HIV). In the present study, optically active S-phenyl-L-cysteine was pre...

    Authors: Lisheng Xu, Xingtao Zhang, Guizhen Gao and Sun Yue

    Citation: BMC Biotechnology 2019 19:49

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  2. Content type: Research article

    Engineered vaccine proteins incorporating both antigen and adjuvant components are constructed with the aim of combining functions to induce effective protective immunity. Bacterial flagellin is a strong candi...

    Authors: Casey M. Gries, Rohith R. Mohan, Dimitrios Morikis and David D. Lo

    Citation: BMC Biotechnology 2019 19:48

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  3. Content type: Research article

    Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study wa...

    Authors: Yang Gao, Feng Feng Sang, De Lan Meng, Yi Wang, Wen Tao Ma and De Kun Chen

    Citation: BMC Biotechnology 2019 19:47

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  4. Content type: Research article

    Natamycin is an antifungal polyene macrolide antibiotic with wide applications in health and food industries. Currently, it is the only antifungal food additive with the GRAS status (Generally Regarded as Safe).

    Authors: Elsayed Ahmed Elsayed, Mohamed A. Farid and Hesham A. El-Enshasy

    Citation: BMC Biotechnology 2019 19:46

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  5. Content type: Research article

    Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environ...

    Authors: Ge Zhang, Shugui Li, Yingbo Xu, Juan Wang, Fan Wang, Yuhua Xin, Zhong Shen, Haibo Zhang, Ming Ma and Haobao Liu

    Citation: BMC Biotechnology 2019 19:45

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  6. Content type: Research article

    Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR)...

    Authors: Alexandre Juillerat, Diane Tkach, Brian W. Busser, Sonal Temburni, Julien Valton, Aymeric Duclert, Laurent Poirot, Stéphane Depil and Philippe Duchateau

    Citation: BMC Biotechnology 2019 19:44

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  7. Content type: Research article

    Proteases are hydrolytic enzymes that catalyze peptide linkage cleavage reactions at the level of proteins and peptides with different degrees of specificity. This group draws the attention of industry. More t...

    Authors: Maroua Omrane Benmrad, Sondes Mechri, Nadia Zaraî Jaouadi, Mouna Ben Elhoul, Hatem Rekik, Sami Sayadi, Samir Bejar, Nabil Kechaou and Bassem Jaouadi

    Citation: BMC Biotechnology 2019 19:43

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  8. Content type: Research article

    Artificial Mitochondrial Transfer or Transplant (AMT/T) can be used to reduce the stress and loss of viability of damaged cells. In MitoCeption, a type of AMT/T, the isolated mitochondria and recipient cells a...

    Authors: Francisco Cabrera, Mayra Ortega, Francesca Velarde, Eliseo Parra, Stephany Gallardo, Diego Barba, Lina Soto, Gabriela Peña, Luis Alberto Pedroza, Christian Jorgensen, Maroun Khoury and Andrés Caicedo

    Citation: BMC Biotechnology 2019 19:42

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  9. Content type: Research article

    Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very compe...

    Authors: Bruna S. Fernandes, Oscar Dias, Gisela Costa, Antonio A. Kaupert Neto, Tiago F. C. Resende, Juliana V. C. Oliveira, Diego M. Riaño-Pachón, Marcelo Zaiat, José G. C. Pradella and Isabel Rocha

    Citation: BMC Biotechnology 2019 19:41

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  10. Content type: Software

    Natural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current of...

    Authors: Laurence O. W. Wilson, Sara Hetzel, Christopher Pockrandt, Knut Reinert and Denis C. Bauer

    Citation: BMC Biotechnology 2019 19:40

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  11. Content type: Research article

    Aerobic side chain degradation of phytosterols by actinobacteria is the basis for the industrial production of androstane steroids which are the starting materials for the synthesis of steroid hormones. A nati...

    Authors: Eugeny Y. Bragin, Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Dmitry V. Dovbnya and Marina V. Donova

    Citation: BMC Biotechnology 2019 19:39

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  12. Content type: Research article

    Anti-Tumor Necrosis Factor-alpha therapy has become clinically important for treating inflammatory bowel disease. However, the use of conventional immunotherapy requires a systemic exposure of patients and col...

    Authors: Maria José Chiabai, Juliana Franco Almeida, Mariana Gabriela Dantas de Azevedo, Suelen Soares Fernandes, Vanessa Bastos Pereira, Raffael Júnio Araújo de Castro, Márcio Sousa Jerônimo, Isabel Garcia Sousa, Leonora Maciel de Souza Vianna, Anderson Miyoshi, Anamelia Lorenzetti Bocca, Andrea Queiroz Maranhão and Marcelo Macedo Brigido

    Citation: BMC Biotechnology 2019 19:38

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  13. Content type: Research article

    CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly cont...

    Authors: Bent Larsen Petersen, Svenning Rune Möller, Jozef Mravec, Bodil Jørgensen, Mikkel Christensen, Ying Liu, Hans H. Wandall, Eric Paul Bennett and Zhang Yang

    Citation: BMC Biotechnology 2019 19:36

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  14. Content type: Research article

    Viral vectors are invaluable tools to transfer genes and/or regulatory sequences into differentiated cells such as pancreatic cells. To date, several kinds of viral vectors have been used to transduce differen...

    Authors: Olivier Albagli, Alicia Maugein, Lukas Huijbregts, Delphine Bredel, Géraldine Carlier, Patrick Martin and Raphaël Scharfmann

    Citation: BMC Biotechnology 2019 19:35

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  15. Content type: Methodology article

    In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more tha...

    Authors: Sin-Yeang Teow, Kitson Liew, Mohd Firdaus Che Mat, Marini Marzuki, Norazlin Abdul Aziz, Tai-Lin Chu, Munirah Ahmad and Alan Soo-Beng Khoo

    Citation: BMC Biotechnology 2019 19:34

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  16. Content type: Research article

    Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify mo...

    Authors: Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark and Margaret C. M. Smith

    Citation: BMC Biotechnology 2019 19:32

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  17. Content type: Research article

    Copy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders. Here we investigate a CNV on chromosome 10q11.22 that spans NPY4R, the gene ...

    Authors: Kateryna Shebanits, Torsten Günther, Anna C. V. Johansson, Khurram Maqbool, Lars Feuk, Mattias Jakobsson and Dan Larhammar

    Citation: BMC Biotechnology 2019 19:31

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  18. Content type: Methodology article

    The potassium dichromate oxidation method used in determination of alcohols in fermentation has two major disadvantages. This method cannot be used to determine alcohols in raw fermentation broth samples, whic...

    Authors: Peng Zhang, Hao Hai, Dongxu Sun, Weihua Yuan, Weijie Liu, Ruru Ding, Mengting Teng, Lin Ma, Jun Tian and Caifa Chen

    Citation: BMC Biotechnology 2019 19:30

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  19. Content type: Research article

    Several types of phospholipases have been described in phospholipids modification. The majority of phospholipase D (PLD) superfamily members can catalyze two separate reactions: the hydrolysis of phospholipids...

    Authors: Haiyang Zhang, Wenqin Chu, Jianan Sun, Zhen Liu, Wen-can Huang, Changhu Xue and Xiangzhao Mao

    Citation: BMC Biotechnology 2019 19:29

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  20. Content type: Research article

    In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumum...

    Authors: Tao Yu, Chunxia Qiao, Ming Lv and Luqun Tang

    Citation: BMC Biotechnology 2019 19:28

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  21. Content type: Methodology article

    The current limitations of conventional BCG vaccines highlights the importance in developing novel and effective vaccines against tuberculosis (TB). The utilization of probiotics such as Lactobacillus plantarum f...

    Authors: Anhar Danial Mustafa, Jeevanathan Kalyanasundram, Sarah Sabidi, Adelene Ai-Lian Song, Maha Abdullah, Raha Abdul Rahim and Khatijah Yusoff

    Citation: BMC Biotechnology 2019 19:27

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  22. Content type: Research article

    Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expr...

    Authors: M. Alejandro Carballo-Amador, Edward A. McKenzie, Alan J. Dickson and Jim Warwicker

    Citation: BMC Biotechnology 2019 19:26

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  23. Content type: Research article

    The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the effic...

    Authors: Kayla Carey, Junghyun Ryu, Kyungjun Uh, Andrea J. Lengi, Sherrie Clark-Deener, Benjamin A. Corl and Kiho Lee

    Citation: BMC Biotechnology 2019 19:25

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  24. Content type: Research article

    Increasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B

    Authors: Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen and Guohao He

    Citation: BMC Biotechnology 2019 19:24

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  25. Content type: Research article

    The gene transduction efficiency of adenovirus to hematopoietic cells, especially T lymphocytes, is needed to be improved. The purpose of this study is to improve the transduction efficiency of T lymphocytes b...

    Authors: Yun Lv, Feng-Jun Xiao, Yi Wang, Xiao-Hui Zou, Hua Wang, Hai-Yan Wang, Li-Sheng Wang and Zhuo-Zhuang Lu

    Citation: BMC Biotechnology 2019 19:23

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  26. Content type: Research article

    The importance of the accessory enzymes such as α-L-arabinofuranosidases (AFases) in synergistic interactions within cellulolytic mixtures has introduced a paradigm shift in the search for hydrolytic enzymes. ...

    Authors: Brent Fortune, Sizwe Mhlongo, Leonardo Joaquim van Zyl, Robert Huddy, Mariette Smart and Marla Trindade

    Citation: BMC Biotechnology 2019 19:22

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  27. Content type: Research article

    A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in...

    Authors: Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, Dawn M. Kilkenny and Julie Audet

    Citation: BMC Biotechnology 2019 19:21

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  28. Content type: Methodology article

    Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable f...

    Authors: Régis P. Lemaitre, Aliona Bogdanova, Barbara Borgonovo, Jeffrey B. Woodruff and David N. Drechsel

    Citation: BMC Biotechnology 2019 19:20

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  29. Content type: Research article

    Recently, it has been indicated that anaerobic co-digestion of waste activated sludge with other waste streams at wastewater treatment plants is a promising strategy for enhancing methane production and materi...

    Authors: Seyed Mostafa Hallaji, Mohammad Kuroshkarim and Seyede Parvin Moussavi

    Citation: BMC Biotechnology 2019 19:19

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  30. Content type: Methodology article

    The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editi...

    Authors: Soumaya Najah, Corinne Saulnier, Jean-Luc Pernodet and Stéphanie Bury-Moné

    Citation: BMC Biotechnology 2019 19:18

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  31. Content type: Research article

    Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The organism has been proposed to be a suitable organism...

    Authors: Alexandria T. N. Holland, Michael J. Danson and Albert Bolhuis

    Citation: BMC Biotechnology 2019 19:17

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  32. Content type: Research article

    Norcantharidin (NCTD), a demethylated derivative of cantharidin (defensive toxin of blister beetles), has been reported to exhibit insecticidal activity against various types of agricultural pests. However, NC...

    Authors: Liya Zeng, Yongchang Liu, Jun Pan and Xiaowen Liu

    Citation: BMC Biotechnology 2019 19:16

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  33. Content type: Research article

    Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To exte...

    Authors: Guilherme Souza Prado, Pingdwende Kader Aziz Bamogo, Joel Antônio Cordeiro de Abreu, François-Xavier Gillet, Vanessa Olinto dos Santos, Maria Cristina Mattar Silva, Jean-Paul Brizard, Marcelo Porto Bemquerer, Martine Bangratz, Christophe Brugidou, Drissa Sérémé, Maria Fatima Grossi-de-Sa and Séverine Lacombe

    Citation: BMC Biotechnology 2019 19:15

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  34. Content type: Research article

    Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical...

    Authors: Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim and Kwang-Chul Kwon

    Citation: BMC Biotechnology 2019 19:13

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  35. Content type: Correction

    Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below:

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

    Citation: BMC Biotechnology 2019 19:12

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    The original article was published in BMC Biotechnology 2019 19:5

  36. Content type: Research article

    Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study ...

    Authors: Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar and Waldemar Rymowicz

    Citation: BMC Biotechnology 2019 19:11

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  37. Content type: Research article

    Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC...

    Authors: Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang and Weibing Xu

    Citation: BMC Biotechnology 2019 19:10

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  38. Content type: Research article

    Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system...

    Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang

    Citation: BMC Biotechnology 2019 19:9

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  39. Content type: Research article

    We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter...

    Authors: Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani and Vadood Razavilar

    Citation: BMC Biotechnology 2019 19:8

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  40. Content type: Research article

    Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and effici...

    Authors: Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen, Weizhi Ji and Yuyu Niu

    Citation: BMC Biotechnology 2019 19:7

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  41. Content type: Research article

    MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

    Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu

    Citation: BMC Biotechnology 2019 19:6

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  42. Content type: Research article

    Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic or...

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III

    Citation: BMC Biotechnology 2019 19:5

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    The Correction to this article has been published in BMC Biotechnology 2019 19:12

  43. Content type: Research article

    Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent yea...

    Authors: Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid and Supachai Vuttipongchaikij

    Citation: BMC Biotechnology 2019 19:4

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  44. Content type: Research article

    A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterol...

    Authors: Nguyen Thanh Trung, Nguyen Minh Hung, Nguyen Huy Thuan, Nguyen Xuan Canh, Thomas Schweder and Britta Jürgen

    Citation: BMC Biotechnology 2019 19:3

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  45. Content type: Research article

    Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Rece...

    Authors: Kiyoung Eun, Min Gi Park, Yeon Woo Jeong, Yeon Ik Jeong, Sang-Hwan Hyun, Woo Suk Hwang, Sung-Hak Kim and Hyunggee Kim

    Citation: BMC Biotechnology 2019 19:1

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  46. Content type: Research article

    The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manuf...

    Authors: Lissa R. Herron, Clare Pridans, Matthew L. Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel J. Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David A. Hume, Andrew C. Gill and Helen M. Sang

    Citation: BMC Biotechnology 2018 18:82

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