Skip to content

Advertisement

  • Retraction Note
  • Open Access

Retraction Note: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

  • 1,
  • 1, 2,
  • 1,
  • 3,
  • 3,
  • 1, 2,
  • 1, 2,
  • 1,
  • 1, 4,
  • 1,
  • 1,
  • 1,
  • 1,
  • 4,
  • 1,
  • 5,
  • 5,
  • 1,
  • 1 and
  • 1Email author
BMC Biotechnology201313:836

https://doi.org/10.1186/1472-6750-13-57

  • Received: 12 July 2013
  • Accepted: 12 July 2013
  • Published:

The original article was published in BMC Biotechnology 2011 11:2

Retraction

The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.

Notes

Authors’ Affiliations

(1)
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague, Czech Republic
(2)
Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-128 40 Prague, Czech Republic
(3)
Centre of Biocatalysis and Biotransformation, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Zámek 136 373 33, Nové Hrady, Czech Republic
(4)
Department of Chemistry, Chemical Engineering and Materials, University of L’Aquila, Via Campo di Pile - Zona industriale di Pile, I-67100 L’Aquila, Italy
(5)
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského náměstí 2, CZ-162 06 Prague, Czech Republic

References

  1. Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 2011, 11:2.View ArticleGoogle Scholar

Copyright

© Kaplan et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement