- Retraction Note
- Open Access
Retraction Note: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
BMC Biotechnology volume 13, Article number: 836 (2013)
The authors have retracted this article  due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.
Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 2011, 11:2.
Original article can be found online at http://dx.doi.org/10.1186/1472-6750-11-2.
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Kaplan, O., Bezouska, K., Plihal, O. et al. Retraction Note: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 13, 836 (2013). https://doi.org/10.1186/1472-6750-13-57