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Retraction Note: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

BMC Biotechnology volume 13, Article number: 836 (2013) Cite this article

The Original Article was published on 06 January 2011

Retraction

The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.

References

  1. Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 2011, 11:2.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20, Prague, Czech Republic

    Ondrej Kaplan, Karel Bezouska, Ondrej Plihal, Ondrej Vanek, Daniel Kavan, Oldrich Benada, Anna Malandra, Ondrej Sveda, Alicja B Vesela, Anna Rinagelova, Kristyna Slamova, Jurgen Felsberg, Michael Kotik, Vladimir Kren & Ludmila Martinkova

  2. Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-128 40, Prague, Czech Republic

    Karel Bezouska, Ondrej Vanek & Daniel Kavan

  3. Centre of Biocatalysis and Biotransformation, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Zámek 136 373 33, Nové Hrady, Czech Republic

    Rudiger Ettrich & Natallia Kulik

  4. Department of Chemistry, Chemical Engineering and Materials, University of L’Aquila, Via Campo di Pile - Zona industriale di Pile, I-67100, L’Aquila, Italy

    Anna Malandra & Maria Cantarella

  5. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského náměstí 2, CZ-162 06, Prague, Czech Republic

    Jarmila Duskova & Jan Dohnalek

Authors
  1. Ondrej Kaplan
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  2. Karel Bezouska
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  3. Ondrej Plihal
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  4. Rudiger Ettrich
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  5. Natallia Kulik
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  6. Ondrej Vanek
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  7. Daniel Kavan
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  8. Oldrich Benada
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  9. Anna Malandra
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  10. Ondrej Sveda
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  11. Alicja B Vesela
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  12. Anna Rinagelova
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  13. Kristyna Slamova
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  14. Maria Cantarella
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  15. Jurgen Felsberg
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  16. Jarmila Duskova
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  17. Jan Dohnalek
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  18. Michael Kotik
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  19. Vladimir Kren
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  20. Ludmila Martinkova
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Corresponding author

Correspondence to Ludmila Martinkova.

Additional information

Original article can be found online at http://dx.doi.org/10.1186/1472-6750-11-2.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kaplan, O., Bezouska, K., Plihal, O. et al. Retraction Note: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 13, 836 (2013). https://doi.org/10.1186/1472-6750-13-57

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  • DOI: https://doi.org/10.1186/1472-6750-13-57

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BMC Biotechnology

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