A process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol
© Prakash et al; licensee BioMed Central Ltd. 2010
Received: 4 February 2010
Accepted: 30 June 2010
Published: 30 June 2010
Substituted catechols are important precursors for large-scale synthesis of pharmaceuticals and other industrial products. Most of the reported chemical synthesis methods are expensive and insufficient at industrial level. However, biological processes for production of substituted catechols could be highly selective and suitable for industrial purposes.
We have optimized a process for bio-catalytic production of 3-substituted catechols viz. 3-nitrocatechol (3-NC) and 3-methylcatechol (3-MC) at pilot scale. Amongst the screened strains, two strains viz. Pseudomonas putida strain (F1) and recombinant Escherichia coli expression clone (pDTG602) harboring first two genes of toluene degradation pathway were found to accumulate 3-NC and 3-MC respectively. Various parameters such as amount of nutrients, pH, temperature, substrate concentration, aeration, inoculums size, culture volume, toxicity of substrate and product, down stream extraction, single step and two-step biotransformation were optimized at laboratory scale to obtain high yields of 3-substituted catechols. Subsequently, pilot scale studies were performed in 2.5 liter bioreactor. The rate of product accumulation at pilot scale significantly increased up to ~90-95% with time and high yields of 3-NC (10 mM) and 3-MC (12 mM) were obtained.
The biocatalytic production of 3-substituted catechols viz. 3-NC and 3-MC depend on some crucial parameters to obtain maximum yields of the product at pilot scale. The process optimized for production of 3-substituted catechols by using the organisms P. putida (F1) and recombinant E. coli expression clone (pDTG602) may be useful for industrial application.
Precursors are essential starting material for the production of a large number of fine chemicals, antibiotics and flavor compounds. Catechols and their derivatives have wide industrial applications as they serve as precursors for large-scale synthesis of industrial and agricultural products [1, 2]. Some catechols and their derivatives are used in manufacturing of synthetic flavors such as vanillin and fragrance . A total of ~50% catechols are used in the synthesis of pesticides such as carbofuran, propouxur etc. . They are also used as reagents in photography, polymerization inhibitors, dying industries for the production of rubber and plastic . Substitutions on the 3 position of catechols are of particular interest due to their potential use as precursors in the synthesis of a wide range of pharmaceuticals including adrenergic catecholamines, biogenic amines and L-DOPA (used in hospitals for anesthesia) [6, 7]. There are only a few world wide sources of 3-NC production and it is highly priced at app. $20,000/g . Chemical synthesis of 3-substituted catechols is difficult due to low regio- selectivity and low stability of the product (the product is oxidized faster than the substrate). Stereo-selectivity in chemical synthesis of these catechols is also inadequate. In addition, chemical synthesis involves multiple reaction steps and consequently the recovery of chemically synthesized catechols on an industrial scale is rather poor [1, 7]. Chemical synthesis of catechols also results a mixture of both 3- and 4- substituted catechols thereby increasing the cost of downstream processing enormously [9, 10].
It has been established that microorganisms are capable of carrying out many enzymatic reactions that can be highly selective and/or specific and therefore, can be utilized for the production of 3-substituted catechols [11–13]. Till date there are only a few reports on bio-catalytic production of substituted catechols. Chae et al. , applied aqueous/organic two-phase reaction system for the production of catechols by using immobilized resting cells of P. putida. In another report, Boshoff et.al , investigated accumulation of catechols using membrane-immobilized polyphenol oxidase with two sequential cresolase and catecholase reactions. Vardar et al. , demonstrated formation of substituted nitrocatechol by oxidation of nitrobenzene using a toluene-o-xylene monooxygenase protein of Pseudomonas stutzeri OX1. The major drawback suggested by the above studies are that, although a number of compounds are transformed or degraded via formation of catechols as a major intermediate, however, their accumulation is quite low. Further, there are virtually no reports in our knowledge available on bio-catalytic production of 3-substituted catechols at pilot scale.
The present work describes a process optimization for the production of 3-substituted catechols at pilot scale using P. putida (F1) and recombinant E. coli expression clone (pDTG602). The process was initially optimized for the production of 3-substituted catechols on laboratory scale and subsequently at pilot scale studies were also performed using 2.5 liter bioreactors to maximize the product yields. The results obtained during the present study indicate the purity/selectivity and increased products yield as well.
Biological and chemical materials
Strain P. putida F1 (ATCC No. 700007) was procured from American Type Culture Collection (ATCC) , USA. A toluene transforming E. coli recombinant expression clone (pDTG602) was a generous gift from Prof. G. J. Zylstra (Department of Biochemistry and Microbiology, Rutgers Universtiy, NJ. USA). 3-Nitrophenol, toluene, 3-nitrocatechol, 3-methylcatechol and p-toluidine were obtained from Sigma Aldrich Corp. (St. Louis, MO. USA). All other chemicals used were of highest purity grade available locally.
Bacterial strains and growth conditions
During the present study the soil samples from pesticide contaminated sites were screened for microorganisms capable of degrading/transforming 3-NP and toluene isolated by enrichment-culture techniques. Briefly, 1 g of fresh contaminated soil resuspended in 50 ml minimal media (MM) supplemented with 10 mM of glucose and lower selective concentration (0.5 mM) of 3-NP and vapors of toluene, incubated at 30°C overnight at 150 rpm. The isolated organisms were grown in enriched source medium such as Nutrient broth (NB) or Luria Broth (LB) or low nutrients MM containing 3-NP and/or toluene as the biotransformation substrates. The MM was used had the following composition (per liter) Na2HPO4: 4.0 g; KH2PO4: 2.0 gm; MgSO4: 0.8 gm; (NH4)2SO4: 0.8 gm; yeast extract; 1.00 gm and trace element solution Al(OH)3: 0.1 gm; SnCl2: 0.5 gm; KI:0.5 gm; LiCl: 0.05 gm; MnSO4.4H2O: 0.08 gm; H3BO3: 0.5 gm; ZnSO4.7H2O: 0.1 gm; CoCl2.6H2O: 0.1 gm; NiSO4.6H2O: 0.1 gm; BaCl2:0.05 gm; (NH4)6Mo7O24.4H2O: 0.05 gm. The MM was prepared as described earlier by Prakash et al. . Incubations were carried out at optimized temperature, pH and substrate concentration with aeration. Bacterial growth was determined by monitoring the OD600 spectrophotometrically (Lambda EZ-201 UV-Vis Spectrophotometer, Perkin-Elmer, USA).
Chromogenic assay for detection of catechols
Bacterial isolates obtained from the enrichment culture technique were screened for the production of 3-substituted catechols including wild type strains P. putida F1 and recombinant E. coli expression clone (pDGT602) using a chromogenic plate based assay as described by Parke, . The overnight grown seed cultures were inoculated on MM agar plates containing 3-NP and toluene as biotransformation substrates. The inoculated plates were incubated at 30°C for 24 h. The aliquots of 1-2 ml of p-toluidine (1 M stock solution of p-toluidine prepared in N, N-dimethylformamide) were spread on the O/N grown plates in presence of 0.5 M solution of ferric chloride. The development of deep red-brown precipitates on the plates were indicating the formation of substituted catechols in the medium. The organisms found to be positive for above chromogenic assay were selected for further process optimization for the production of 3-substituted catechol at pilot scale.
Enzymatic assay for biotransformation
Cell extracts of P. putida F1 and recombinant E.coli expression clone were prepared by sonification of the cells for 10 min and the crude extract was centrifuged 10,000 rpm at 4°C for 20 min as described by Kieboom et al. . Protein was determined according to the method as described by Bradford, . The study of enzyme toluene dioxygenase assay by P. putida F1 from 3-NP to 3-NC was performed as described by Gibson et al., de Bont et al. [20, 21].The volume of reaction mixture contained 3-NP (5 mM), 50 mM potassium phosphate buffer (pH 7.0), cell extract (30-40 mg of protein) in a final volume of 1 ml. The reaction was initiated by addition of substrate and reaction mixture was scanned 250-450 nm after every 2 min using a spectrophotometer (Perkin Elmer Lambda EZ201 UV/Vis) at room temperature. The activity of toluene dioxygenase was measured by increasing absorbance at Amax 292 nm indicating the formation of 3-NC. However, the activity of toluene dioxygenase and cis-dihydrodiol dehydrogenase from toluene to 3-MC by clone pDTG602 were carried out by radioactive toluene dioxygenase assays using radiolabelled [14C] toluene (500 μM) in dimethylformamide (specific activity 74.4 μCi/μM). The reaction mixture contained cell extract (20-40 mg of protein), 2 mM NADH, 150 μM ferrous sulphate and 2.5 μl of [14C] toluene in a final volume of 1 ml by 50 mM Bis- Tris propane HCl (pH 6.8) as described by Gibson et al. . Reaction was initiated by addition of [14C] toluene. After 10 min of incubation, 100 μl of reaction mixture was applied to a piece of plastic-backed silica (15 cm by 20 cm) to absorbed metabolite and the sample was dried for 20 min to remove volatile [14C] toluene. The amount of radiolabelled metabolite was formed measured by scintillation counting (Beckman LS6800 scintillation counter, USA). The formation of reaction products were quantified and identified using authentic standard of 3-NC and 3-MC. The solutions were extracted twice at acidic pH 2.0 (6N HCl) with ethyl acetate. The reaction product 3-NC formed was rationally correlated with consumption of the substrate (3-NP) and identified by HPLC. However, the formation of 3-MC was quantified by measuring of a total of 84% of radioactivity (878,000 DPM) by counting nonvolatile14C-labeled metabolites. The transformed metabolite was further identified by GC-MS with EI using authentic standard of 3-MC.
Process optimization for biotransformation
To carry out the transformation process at pilot scale various parameters were initially optimized at laboratory shake flask scale (250 ml volume) in order to produce 3- substituted catechols in the medium using P. putida strains F1 and the recombinant E. coli expression clone pDTG602.
Optimization of different conditions
Different media such as NB, LB and MM with different concentration of glucose and sodium succinate (5 mM, 10 mM and 20 mM), varying pH (6.5, 7.5, 8.0 and 8.5) and incubation temperature (28°C, 30°C, 35°C and 37°C) were tested at laboratory scale for optimum production of 3-NC and 3-MC. Similarly, different range of aeration (180 rpm, 190 rpm, 200 rpm, 210 rpm and 220 rpm) were tested. Varying concentration of 3-NP (.5 mM, 1 mM, 2 mM, 3 mM, 4 mM, 5 mM and 6 mM) were also tested. Toluene was provided to the growing cultures in the form of saturated vapors. A range of inoculums size (1%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5% and 5.0%) were also attempted to obtain optimum yields of the desired products.
Effect of induced growth on transformation
The effect of induction on transformation was also optimized using large cell mass of strains P. putida F1 and recombinant E. coli expression clone (pDTG602). For pre-induction, cells of strains F1 was grown in 250 ml of MM supplemented with 15 mM glucose. However, the cells of clone (pDTG602) was grown in LB (OD600 ~1.0). The pre-grown cells were then re-suspended in 50 ml MM containing 5 mM of 3-NP or toluene saturated vapors as biotransformation substrate in order to evaluate the effect of induction on transformation efficiency.
Down stream extraction of the transformed products 3-NC and 3-MC was carried out as described by Husken et al. . Briefly, process samples (~10 ml) were collected at different time intervals from the transformation broth. The time points selected for samples collection were rationalized by chromogenic spot test (200 μl sample + 100 μl p-toluidine + 100 μl of ferric chloride) in microtiter plate. The intensity of the visible red- brown color and concentrations of 3-substituted catechols formation were measured at 510 nm using standard calibration curve of 3-NC and 3-MC. Collected samples were centrifuged to separate the cell mass and supernatant. The medium supernatants were solvent extracted twice with equal volumes of ethyl acetate/diethyl ether/octanol to separate the organic components of the samples from non-organic contents. Finally, the collected organic component of the samples were evaporated to dryness under nitrogen flow using TurboVap-II (Caliper Life Sciences, USA) and re-suspended in methanol for chromatographic analysis.
Qualitative and quantitative analysis
Qualitative and quantitative analyses of the transformed products were performed using different analytical techniques such as Thin Layer Chromatography (TLC), Gas Chromatography (GC) and High performance Liquid Chromatography (HPLC). For TLC extracted samples were resuspended in adequate amount of methanol and analyzed on a silica gel coated TLC aluminum 60 F254 plates (20 × 20 cm thickness, E. Merck, Germany). The solvent system used was toluene: ethyl acetate: benzene in a ratio of 60:35:5. The TLC sheets were analyzed under near and far UV electromagnetic radiation. Rf values of individual spots were calculated according to the standard method and compared with the Rf values of the known compounds. GC analysis was carried out using flame ionization detector (FID) and a HP-1 (100% dimethyl polysiloxane) capillary column (30 m × 0.25 mm) (Perkin Elmer, MA, USA). The GC parameters used were as follows: injector temperature, 250°C; oven temperature, gradient 150- 230°C over 15 min; and detector temperature, 200°C. HPLC analysis was performed on Water 600 (Milford, MA, USA) equipped with a 996 photodiode array detector operated at 190-600 nm. Separation was carried out with C-18 reverse phase column (5 um, 4.6×250 nm; waters spherisorb ODS2) using as mobile phase of 0.1% tri-fluroacetic acid (TFA) in methanol: water (1:1) for 3-MC and 0.1% glacial acetic acid in methanol: water (1:1) for 3-NC at an isocratic flow rate of 1.5 ml/min.
Pilot scale biotransformation
For the bioconversion of toluene to 3-MC, a single step batch bioreactor was optimized (Figure 1b). An overnight pre-grown induced culture of pDTG602 was inoculated in 2.5 liter transformation broth. The bioreactor was then sparged with toluene vapors to saturate the air to be utilized as biotransformation substrate. The biotransformation process was performed for 48 h at 37°C for optimum production of 3-MC. The procedures for minimization of bioreactor toxicity, extraction of the column and analysis of product were identical as 3-NC production.
Results and Discussion
Screening of catechol producing strains
Several strains belonging to genus Bacillus, Kocuria, Ralstonia, Micrococcus and Pseudomonas were obtained from the enrichment culture techniques. These isolated organisms were tested for their ability to convert 3-NP and toluene into 3-substituted catechol including wild type strain P. putida F1 and E. coli expression clone (pDTG602). Among the screened organisms only strain F1 and expression clone pDTG602 were found to be metabolizing 3-NP and toluene respectively. The organism F1 utilizing 3-NP as sole source of carbon and energy. However the expression clone (pDTG602) only transformed toluene into 3-MC in presence of growth supporting additional carbon source glucose. The selected strains were also tested for chromogenic assay and found to be strongly positive for the presence of 3-substituted catechols in the degradation pathway of 3-NP and toluene.
To optimize the biotransformation processes at pilot scale certain conditions such as medium composition, amount of nutrients, pH, temperature, aeration, inoculums size, substrate concentration, culture volume, single and two step biotransformation processes were optimized at shake flask scale for optimum production of 3-substituted catechols. The optimized conditions available from the shake flask (250 ml) were tested at (2.5 liter) pilot scale in order to obtained high yields of the target compounds.
Optimization of different conditions
Effect of induction on transformation ability
Downstream extraction process was developed to enhance the recovery as well as purity of 3-NC and 3-MC from the biotransformation medium. Various solvents such as ethyl acetate, diethyl ether and octanol were tested for recovery of the final transformed products. The solvent octanol was found to be the suitable for maximum % recovery of the substituted catechols extraction. The results indicated that ~90% recovery of 3-NC and up to ~95% of 3-MC were obtained using octanol solvent during the process. However, the poor recovery of the products were obtained in ethyl acetate 60% of 3-NC; 70% of 3-MC. Similarly 40% recovery of 3-NC and 35% of 3-MC were obtained using solvent diethyl ether. The mass - fragmentation analysis of transformed product revealed that molecular ions peak at m/z 155* (100) (m+1) 125 (31), 109 (25), 81 (51), 55(61) and 53 (87) and m/z 124* (100), 123 (30), 106(9), 78 (40), 77(10), 51(9) and 39(11), which corresponds to 99% pure authentic standard of 3-NC and 3-MC respectively. The transformed products dissolved better in octanol than the other solvents and therefore, it was used for subsequent downstream extraction experiments. The optimized conditions/parameters available from the laboratory scale indicated the accuracy and applicability of 3-substituted catechol at pilot scale. Therefore, the down stream process developed during the present study is a cost effective method and has potential to produce maximum yields of the substituted catechols at pilot level.
Qualitative and quantitative analysis
In order to check the quality and quantity of the transformed products 3-NC and 3-MC TLC study was initially performed using authentic standards of 3-substituted catechols. The Rf values of the samples drawn at different time intervals were exactly matching with standards of 3-NC and 3-MC. TLC plate was also sprayed with Folin Ciocalteu reagent an immediate development of blue coloration was apparent which indicated presence of diphenolic compounds . Analysis with GC did not work well (data not shown). The possible explanation for this could be that catechols are thermally unstable and they are destroyed when subjected to high temperatures applicable in GC analysis. Amongst the three chromatographic methods HPLC performed the best for analysis the transformed products. Therefore, the formation of 3-NC and 3-MC were further confirmed by HPLC analysis. Based upon the HPLC retention time and spectral analysis the production of substituted catechols were identified at 2.123 min, 296 nm for 3-NC and 4.656 min, 274 nm for 3-MC in the spent medium during the biotransformation process.
Pilot scale biotransformation
Biological production of substituted catechols depends on the certain conditions such as substrate, bioreactor, biomass, toxicity of substrate and product, downstream processing costs etc. The effective combination of microbial strain used and appropriate process-optimized conditions offer high productivities of the substituted catechols. The process optimization for microbial production of 3-substituted catechols in this study can serve wide industrial applications.
We are thankful to Department of Biotechnology (DBT) and Council of Scientific and Industrial Research (CSIR), Govt. of India for financial support. This is IMTECH communication number 063/2009.
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