Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163
- Iris Delrue1,
- Hanne Van Gorp1,
- Jan Van Doorsselaere2,
- Peter L Delputte†1 and
- Hans J Nauwynck†1Email author
© Delrue et al; licensee BioMed Central Ltd. 2010
Received: 14 February 2010
Accepted: 29 June 2010
Published: 29 June 2010
Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145. The latter is currently used for vaccine production. However, since virus entry in Marc-145 cells is different compared to entry in primary macrophages, specific epitopes associated with virus entry could potentially alter upon growth on Marc-145 cells. To avoid this, we constructed CHO and PK15 cell lines recombinantly expressing the PRRSV receptors involved in virus entry into macrophages, sialoadhesin (Sn) and CD163 (CHOSn-CD163 and PK15Sn-CD163) and evaluated their potential for production of PRRSV.
Detailed analysis of PRRSV infection revealed that LV and VR-2332 virus particles could attach to and internalize into the CHOSn-CD163 and PK15Sn-CD163 cells. Initially, this occurred less efficiently for macrophage grown virus than for Marc-145 grown virus. Upon internalization, disassembly of the virus particles was observed. The two cell lines could be infected with PRRSV strains LV and VR-2332. However, it was observed that Marc-145 grown virus infected the cells more efficiently than macrophage grown virus. If the cells were treated with neuraminidase to remove cis-acting sialic acids that hinder the interaction of the virus with Sn, the amount of infected cells with macrophage grown virus increased. Comparison of both cell lines showed that the PK15Sn-CD163 cell line gave in general better results than the CHOSn-CD163 cell line. Only 2 out of 5 PRRSV strains replicated well in CHOSn-CD163 cells. Furthermore, the virus titer of all 5 PRRSV strains produced after passaging in PK15Sn-CD163 cells was similar to the virus titer of those strains produced in Marc-145 cells. Analysis of the sequence of the structural proteins of original virus and virus grown for 5 passages on PK15Sn-CD163 cells showed either no amino acid (aa) changes (VR-2332 and 07V063), one aa (LV), two aa (08V194) or three aa (08V204) changes. None of these changes are situated in known neutralizing epitopes.
A PRRSV susceptible cell line was constructed that can grow virus to similar levels compared to currently available cell lines. Mutations induced by growth on this cell lines were either absent or minimal and located outside known neutralizing epitopes. Together, the results show that this cell line can be used to produce vaccine virus and for PRRSV virus isolation.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirales [1, 2] causing major economic losses in the pig industry worldwide . PRRSV infection may result in reproductive failure in sows and is involved in the porcine respiratory disease complex (PRDC) [4–9].
In vivo, the virus infects a subpopulation of tissue macrophages [10–13]. In vitro, efficient PRRSV replication is only observed in primary pig macrophages (e.g. alveolar macrophages) , differentiated monocytes  or African green monkey kidney derived cells, such as Marc-145 [14, 16]. Infection of macrophages, the natural host cell of PRRSV, occurs via a few similar but also different receptors compared to infection of Marc-145 cells . PRRSV first attaches to macrophages via heparan sulphate , then the virus is internalized via sialoadhesin (Sn) . CD163 is also involved in infection of macrophages, probably at the stage of virus disassembly . PRRSV infection of Marc-145 cells occurs via binding to a heparin-like molecule as a first step . The nucleocapsid of PRRSV is described to bind to the intermediate filament vimentin, which is suggested to mediate transport of the virus to the cytosol . CD151 may be involved in fusion of the viral envelope and the endosome, but the precise mechanism is yet unknown . CD163 is also essential for PRRSV infection of Marc-145 cells, but its role in this process is still unclear .
Currently, PRRS vaccine virus is produced in Marc-145 cells. However, since virus entry in Marc-145 cells is different compared to entry in primary macrophages  and because adaptation is needed for growth on Marc-145 cells , it is possible that specific epitopes associated with virus neutralization are lost or modified. Although virus production in primary macrophages would be ideal to avoid adaptation, these cells cannot be used because of batch variation, risk of contamination with other pathogens present in the macrophages isolated from pigs and high production costs. Previous results in our lab showed that non-permissive cells transiently transfected with Sn only sustained internalization, but not infection . Non-permissive cells transiently transfected with CD163 allow a low level of infection depending on the cell type used . Co-expression of both Sn and CD163 is the most efficient for PRRSV infection in different cell lines evaluated .
To avoid the problems associated with PRRS vaccine virus production in other cell types, the aim of this study was to construct cell lines that recombinantly express Sn, the receptor that mediates PRRSV attachment to and internalization into macrophages [19, 27] and CD163, which is most probably involved in virus disassembly in macrophages . Both Sn and CD163 are needed to make a PRRSV susceptible cell line for virus production that mimics the natural entry pathway in macrophages.
Cells, viruses and plasmids
CHO-K1 cells were cultivated in F12 medium and PK15 cells in Dulbecco Modified Eagle Medium (D-MEM). Both media were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1 mM non-essential amino acids, 1 mM sodium pyruvate and a mixture of antibiotics. The cells were maintained in a humidified 5% CO2 atmosphere at 37°C. Macrophages cultivated in medium containing RPMI-1640, 10% FBS, 2 mM L-glutamine, 1 mM non-essential amino acids, 1 mM sodium pyruvate and a mixture of antibiotics were used for titration. A pcDNA3.1D/V5-HisTOPO plasmid containing Sn cDNA and geneticine resistance gene  and a pBUD plasmid with CD163 cDNA and zeocin resistance gene were used for transfection. To construct the pBUD plasmid containing CD163, CD163 from a pcDNA3.1D/V5-HisTOPO plasmid containing CD163 , was cloned into a pBUD plasmid via restriction site HindIII and XbaI. The European prototype PRRSV strain Lelystad virus (LV), grown on Marc-145 cells and macrophages , the American prototype PRRSV strain VR-2332 grown on Marc-145 cells , and three recent Belgian isolates, belonging to the European type, grown on macrophages (07V063, 08V204 and 08V194) were used for inoculation.
Transfection and selection
CHO-K1 and PK15 cells were transfected with FuGENE 6 (Roche) or Lipofectamine Plus (Invitrogen, Merelbeke, Belgium) respectively, according to the manufacturer's instructions. CHO-K1 and PK15 cells were first transfected with a plasmid containing the Sn cDNA and geneticine resistance gene. The cells were single cell cloned and selected for Sn expressing CHO and PK15 cells with geneticine (200 μg/ml, GIBCO). Afterwards, the obtained CHOSn and PK15Sn cells were transfected with a plasmid containing the CD163 cDNA and zeocin resistance gene and single cell cloned. For the selection of CHOSn and PK15Sn cells expressing CD163, zeocin (200 μg/ml, Invitrogen, Merelbeke, Belgium) was used.
Screening of cells expressing Sn and CD163 by immunofluorescence staining
Transfected CHO-K1 and PK15 cells were fixed with methanol and stained with primary monoclonal antibodies (mAb) against Sn (mAb 41D3) [19, 28] and CD163 (mAb 2A10, AbD Serotec) [29, 30]. As a secondary antibody, fluorescein isothiocyanate (FITC)-conjugated goat polyclonal anti-mouse immunoglobulins (Invitrogen, Molecular Probes, Merelbeke, Belgium) were used. Screening of cells expressing Sn and/or CD163 was performed with a fluorescence microscope (Leica Microsystems GmbH, Heidelberg, Germany).
Analysis of PRRSV infection of CHOSn-CD163 and PK15Sn-CD163 cells by immunoperoxidase staining
CHOSn-CD163 and PK15Sn-CD163 cells were seeded in 96-well plates and inoculated with virus. At different time points post inoculation (pi), the cells were fixed with methanol and an immunoperoxidase staining was performed . Briefly, viral antigen positive cells were stained with primary mAb anti-nucleocapsid P3/27  and secondary antibody peroxidase labeled goat anti-mouse immunoglobulin (Ig) (DakoA/S, Glostrup, Denmark). Afterwards, 3-amino-9-ethylcarbazole (AEC) substrate (Sigma, Bornem, Belgium) was added. The amount of infected cells was counted with a light microscope (Olympus Optical Co., Hamburg, Germany).
Attachment, internalization, disassembly and infection of CHOSn-CD163 and PK15Sn-CD163 cells with PRRSV analyzed by immunofluorescence staining
To determine attachment, internalization, disassembly and infection of CHOSn-CD163 and PK15Sn-CD163 cells, the cells were seeded at 200 000 cells/mL and after 2 days of cultivation they were inoculated with virus. The cells were fixed with methanol after 1 hour incubation at 4°C to investigate attachment, since virus is not able to internalize at 4°C. The cells were fixed after 1 hour incubation at 37°C to determine the internalized particles. After 5 hours incubation at 37°C, the cells were fixed to analyze disassembly (disappearance of staining). To analyze infection, the cells were fixed after 24 hours incubation at 37°C. The virus was stained with a primary mAb anti-nucleocapsid P3/27  and a secondary FITC-conjugated goat polyclonal anti-mouse immunoglobulins (Invitrogen, Molecular Probes, Merelbeke, Belgium). Virus particles were counted on images acquired with a TCS SP2 laser scanning spectrum confocal system (Leica Microsystems GmbH, Heidelberg, Germany).
Virus production after passaging in CHOSn-CD163 and PK15Sn-CD163 cells
CHOSn-CD163 and PK15Sn-CD163 cells were seeded at 200 000 cells/mL in Tissue Culture (TC) flasks. After 2 days of cultivation, the cells were initially inoculated with 105 TCID50 of each virus strain. After 3 and 5 days post inoculation (dpi), the supernatant was collected and centrifuged for 30 min at 30 × g at 4°C. The supernatant was stored at -70°C and titrated. Virus titration was performed on 24 hours cultivated alveolar macrophages following the standard procedure . After 3 days of incubation at 37°C, the occurrence of cytopathic effect (CPE) was investigated. Macrophages were fixed at 3 dpi and an immunoperoxidase staining was performed to identify infected cells. The 50% tissue culture infective dose (TCID50) was calculated.
Virus sequencing after passaging virus in PK15Sn-CD163 cells
RNA was extracted from PRRSV passaged 4 times on PK15Sn-CD163 cells using a RNeasy Protect Mini Kit (QIAGEN) and reverse transcribed using random hexamers and MultiScribe Reverse Transcriptase (Applied Biosystems) according to the manufacturer's guidelines.
The primers ORF2a-FW (5'-gtsacaccktatgattacg-3') and ORF2a-REV (5'-tcatrccctattytgcacca-3'), ORF3-FW (5'-agcctacagtacaacaccac-3') and ORF3-REV (5'-agaaaaggcacgcagaaagca-3'), ORF4-FW (5'-cggcccaittccatccigag-3') and ORF4-REV (5'-cattcagctcgcataicgtcaag-3'), ORF5-FW2 (5'-tgcticatttcitgacacc-3') and ORF5-REV1 (5'-accttaagigcitatatc-3'), ORF6FW (5'-taccaactttcttctggac-3') and ORF6REV (5'-acccagcaactggcacag-3'), ORF7-FW (5'- tggcccctgcccaicacg-3') and ORF7-REV (5'- tcgccctaattgaataggtga-3') were used to amplify the different ORFs with Taq Polymerase (Invitrogen, Merelbeke, Belgium). PCR products were treated with Exonuclease I and Antarctic Phosphatase (New England Biolads, Ipswich, USA) and used directly for cycle sequencing with a Big Dye Terminator Cycle sequencing kit V1.1 (Applied Biosystems, Foster City, USA) and PRRSV primers. Cycle sequencing reaction products were purified by ethanol precipitation and separated on an ABI Genetic 310 (Applied Biosystems, Foster City, USA).
The sequences were analyzed and compiled by BlastN and BlastP http://www.ncbi.nlm.nih.gov, and Sixframe, ClustalW, Align (workbench.sdsc.edu). The Genbank accession number are for 07V063 [Genbank:GU737264], 08V204 [Genbank:GU737266] and for 08V194 [Genbank:GU737265].
Construction of CHOSn-CD163 and PK15Sn-CD163 cell line
Effect of cell density and cultivation time of CHOSn-CD163 and PK15Sn-CD163 cells on the susceptibility to PRRSV infection
To determine the effect of cell density and cultivation time of the cells on susceptibility to PRRSV infection, 3 CHOSn-CD163 cell clones (IC5, ID9 and IF3) and 2 PK15Sn-CD163 cell clones (IXH7 and IXA3) were seeded at different cell densities (100 000, 200 000 or 300 000 cells/mL) and inoculated with 50 μL containing 104 TCID50 Marc-145 grown LV, Marc-145 grown VR-2332 or macrophage grown LV at different days post seeding (1, 2 or 3 days post seeding). After 2 dpi, the cells were fixed and stained.
Both CHOSn-CD163 and PK15Sn-CD163 cells showed a low infection rate with macrophage grown virus. The cell density had no influence on infection. There were no differences between clone IC5, ID9 and IF3 for CHOSn-CD163 and between IXH7 and IXA3 for PK15Sn-CD163 cells (Figure 2). Overall, Marc-145 grown virus strains could infect the cells more efficiently than macrophage grown virus strains tested.
Effect of neuraminidase treatment of CHOSn-CD163 and PK15Sn-CD163 cells on the susceptibility to macrophage grown PRRSV infection
The previous section showed that macrophage grown PRRSV infection rate is very low. Previously, it was however shown that infection of macrophages can be enhanced after removal of sialic acid from the cells with neuraminidase, as observed in our lab . Desialylation of macrophages enhances sialoadhesin-mediated lectin activity . Since the interaction between sialic acids on PRRSV and sialoadhesin is important for infection of cells , desialylation of the CHOSn-CD163 and PK15Sn-CD163 cells can probably enhance the amount of infected cells by macrophage grown virus. Therefore, to increase the infection rate of macrophage grown virus infection, 3 CHOSn-CD163 cell clones (IC5, ID9 and IF3) and 2 PK15Sn-CD163 cell clones (IXH7 and IXA3) were seeded at different densities (100 000, 200 000 or 300 000 cells/mL) and were infected at different days post seeding (1, 2 or 3 days post seeding) with 50 μL containing 104 TCID50 macrophage grown LV, after treatment of the cells with neuraminidase to remove cis-acting sialic acids. After 2 dpi, the cells were fixed and stained. The results showed that treatment of the PK15Sn-CD163 cells at 200 000 cell/mL with neuraminidase before inoculation enhanced infection of the cells with macrophage grown virus (Figure 2).
Analysis of PRRSV attachment, internalization, disassembly and infection of CHOSn-CD163 and PK15Sn-CD163 cells
PRRSV infection kinetics on CHOSn-CD163 and PK15Sn-CD163 cells
Virus production in CHOSn-CD163 and PK15Sn-CD163 cells
Virus sequencing after passaging virus in PK15Sn-CD163 cells
As most virus strains grow better on PK15Sn-CD163 cells, this cell line was more interesting to grow vaccine virus than CHOSn-CD163 cells. To produce vaccine virus for inactivated vaccines mutation of structural proteins should not occur. Therefore, PK15Sn-CD163 grown virus was sequenced and compared to the sequence of the original virus. VR-2332 and 07V063 grown on PK15Sn-CD163 cells showed no differences after 5 passages compared to the original virus strain. PK15Sn-CD163 grown LV showed 1 amino acid (aa), 08V194 2 aa and 08V204 3 aa differences compared to macrophage grown virus. For LV the aa change was in ORF4 (N37D). The aa differences of 08V194 were located in ORF2 (E73D) and in ORF5 (N37S). For 08V204 the aa differences were situated in ORF2 (N37D), ORF4 (I121V) and ORF5 (N37D).
PRRSV replicates efficiently in ex vivo cultivated primary macrophages, which are the natural host cells. For vaccine virus production however, this cell type cannot be used, because of batch variation, risk of contamination with other pathogens and high production costs. PRRSV susceptible cell lines, such as the African green monkey derived cell lines, like Marc-145, have the potential to overcome problems associated with the use of primary macrophages, such as up-scaling and safety. However, PRRSV infects Marc-145 cells via a different entry pathway compared to macrophages, which results in adaptation of the virus for growth on Marc-145 cells .
Several non-permissive cells transfected with RNA of PRRSV could produce infectious virus. It is therefore suggested that the susceptibility of cells for PRRSV infection is determined by membrane-associated components . In a previous study, it was shown that non-permissive cells transiently transfected with Sn only sustained internalization, but not infection . Non-permissive cells transiently transfected with CD163 may allow a low level of infection depending on the cell type used . Van Gorp et al. showed that co-expression of both Sn and scavenger receptor CD163 are needed for an efficient PRRSV infection . A virus titer ranging between 102.4 and 105.5 TCID50/mL could be obtained upon PRRSV infection of cells transiently transfected with recombinant Sn and CD163 . Since only a part of the cells were transfected upon transient transfection, it was expected that the virus titer would be higher if stably transfected cell lines could be used. In this study, cell lines that express both recombinant Sn and CD163 (CHOSn-CD163 and PK15Sn-CD163) were made, as both receptors are involved in infection of the natural host cell, the macrophage [20, 27]. The constructed CHOSn-CD163 and PK15Sn-CD163 cell lines were first analyzed for their PRRSV susceptibility. They were both susceptible for PRRSV, because LV and VR-2332 virus attachment, internalization, disassembly and infection occurred in both cell lines.
When analyzing the PRRSV susceptibility, it was shown that primary infection of the cell lines was more efficient with Marc-145 grown virus than macrophage grown virus. The infection rate of macrophage grown virus was very low, most likely because binding and internalization of macrophage grown virus particles into the cell lines was not efficient. The interaction of macrophage grown PRRSV with Sn, the receptor mediating binding to and internalization into the cells, is probably not efficient. This can be due to sialic acids present on the cells interfering with Sn, resulting in competition with sialic acid on PRRSV. This hypothesis is based on the observation that CD33, also a member of the sialoadhesin family, transfected in COS cells were not able to bind to red blood cells, containing sialic acid, unless the COS cells were first treated with sialidase to remove endogenous ligands [36, 37]. Also CHOSn cells showed no binding of red blood cells unless they were treated with neuraminidase .
To investigate if neuraminidase could improve macrophage grown PRRSV infection, the CHOSn-CD163 and PK15Sn-CD163 cells were first treated with Vibrio cholerae neuraminidase (Roche) to remove potential cis-acting sialic acids that could interfere with the sialic acid binding capacity of Sn . This resulted in an increased amount of macrophage grown virus infected cells. These results suggest that the low virus titers are related with a low binding capacity of PRRSV to Sn, because of sialic acid present on the CHOSn-CD163 and PK15Sn-CD163 cells. Marc-145 grown PRRSV will most likely also contain sialic acids that can interfere with Sn. Our hypothesis is that also the production of Marc-145 grown virus can be improved by neuraminidase treatment of the cells and needs to be further investigated.
However, using an expensive product like Vibrio cholerae neuraminidase is not ideal for vaccine production. To avoid this problem, virus is grown via several passages on the cell lines. Normally, titers of 105-107 TCID50/mL can be obtained on Marc-145 cells after 5 to 7 passages [16, 38]. The results show that the PK15Sn-CD163 cells give similar results as a recent macrophage cell line transfected with CD163 . In addition, the PK15Sn-CD163 cells also express Sn, which is important to facilitate virus entry. The macrophage cell line transfected with CD163 also expresses Sn , which confirms that both receptors, Sn and CD163, are important for an efficient virus production. Virus growth on PK15Sn-CD163 cells resulted in higher titers than growth on CHOSn-CD163 cells and the virus titer achieved on PK15Sn-CD163 cells was equal to the titer on Marc-145 cells, which makes the PK15Sn-CD163 cell line an interesting tool for virus production.
It is reported that due to adaptation of PRRSV to Marc-145 cells, mutations in non-structural, but also structural viral proteins may occur [40–42]. For the production of an inactivated virus vaccine, mutations in ORFs encoding viral structural proteins are not desired, since this can influence the induction of a virus neutralizing antibody response. The virus grown on PK15Sn-CD163 cells is expected to show less mutations than after growth on Marc-145 cells, since the PK15Sn-CD163 cells express Sn and CD163, two receptors important in the entry of the virus in macrophages and the entry-associated domains are probably involved in the induction of a neutralizing antibody response. To investigate if virus grown on PK15Sn-CD163 cells showed mutation in ORFs encoding viral structural proteins, ORF2a, 3, 4, 5, 6 and 7 were sequenced. 07V063 and VR-2332 grown on PK15Sn-CD163 cells showed a 100% aa identity with 07V063 grown on macrophages and VR-2332 grown on Marc-145 cells. LV grown on PK15Sn-CD163 cells had 1 aa changed in ORF 4 compared to macrophage grown LV, which resulted in a loss of a putative glycosylation site. The mutation, however, was not situated in a known neutralizing epitope [43, 44]. PK15Sn-CD163 grown 08V194 had 2 aa difference compared to macrophage grown 08V194. There was 1 aa changed in ORF5. This resulted not in a loss of a glycosylation site, but the glycosylation site moved to another place. The mutation was not situated in a known neutralizing epitope . The second aa change was located in ORF2a, however a change from E to D is supposed to have no effect on the protein structure since those aa are similar. For 08V204 grown on PK15Sn-CD163 cells, there was 1 aa changed in ORF2a, 1 aa in ORF4 and 1 aa in ORF5. The mutation in ORF4, however was a change from I to V, which are comparable aa and will not have an influence on the protein structure. The mutation in ORF5 results in a loss of a putative glycosylation site, but is not located in a known neutralizing epitope . The mutation in ORF5 of 08V194 and 08V204 are both on position 37. This position varies among different PRRSV strains and is a not well conserved glycosylation site . These results indicate that the PK15Sn-CD163 cells are useful for production of vaccine virus, but each strain should be investigated for aa changes in the structural proteins before use in inactivated vaccine production. Experiments are ongoing to test the immunogenicity of inactivated PRRSV grown on PK15Sn-CD163 cells and the effect of the minor aa changes on the induction of a protective immunity towards challenge virus.
The observation that all tested strains grow well on the PK15Sn-CD163 cells also suggests that these cells might be useful for virus isolation. Currently Marc-145 cells are used for diagnostics, but it has been shown that not all PRRSV strains can be detected on those cells . Macrophages, the natural host cell of PRRSV, are more efficient for virus isolation . The difference in isolation efficiency between Marc-145 cells and macrophages is suggesting that another receptor next to CD163 is involved in infection as Marc-145 cells and macrophages both express CD163 [20, 24]. The PK15Sn-CD163 cells can be useful for virus isolation, because of the expression of both CD163 and Sn, but needs to be further investigated.
A PRRSV susceptible cell line expressing two major receptors for infection in macrophages, namely Sn and CD163, was constructed. It is shown that virus production from genotype 1 (LV, 3 Belgian strains) and genotype 2 (VR-2332) on the cell line is possible with virus titers being equal to the virus titers achieved on Marc-145 cells. No or only a few mutations occur in the ORFs encoding viral structural proteins and the mutations that occur for some viruses are not situated in known neutralizing epitopes. Therefore it is assumed that an inactivated PRRSV vaccine based on virus grown on the PK15Sn-CD163 cells will be able to induce a proper immune response, but this is currently under investigation. In addition, the PK15Sn-CD163 cells can probably also be used for virus isolation, because of the expression of both Sn and CD163, but this also needs to be confirmed.
The authors would like to thank Carine Boone, Chantal Vanmaercke, Christiane Bracke, Natasha De Corte and Dries Helderweirt for their highly appreciated and excellent technical assistance. This work was funded by the Agricultural Research Program from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-LO 040721).
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