Construction of CHOSn-CD163 and PK15Sn-CD163 cell lines. A) Schematic representation of the construction of CHOSn-CD163 and PK15Sn-CD163 cell lines
To construct a cell line co-expressing Sn and CD163, CHO-K1 or PK15 cells were transfected with a plasmid containing the Sn cDNA and a geneticine resistance gene. The cells were single cell cloned and clones were screened for Sn expressing cells. After selection for geneticine resistance, the obtained CHOSn or PK15Sn cells were transfected with a plasmid containing the CD163 cDNA and a zeocin resistance gene, which allowed selection of cells expressing both Sn and CD163.
B) Immunofluorescence staining of the obtained CHO
cells for Sn and CD163.
Some CHOSn-CD163 clones (IF3, IC5 and ID9) and PK15Sn-CD163 clones (IXA3 and IXH7) are represented with their Sn and CD163 expression.