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  1. Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control...

    Authors: Jana Neuhold, Katharina Radakovics, Anita Lehner, Florian Weissmann, Maria Queralt Garcia, Mari Carmen Romero, Nicholas S. Berrow and Peggy Stolt-Bergner
    Citation: BMC Biotechnology 2020 20:26
  2. Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, howev...

    Authors: Agnieszka Gawin, Helga Ertesvåg, Sine Alise Hartvigsen Hansen, Jostein Malmo and Trygve Brautaset
    Citation: BMC Biotechnology 2020 20:24
  3. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu
    Citation: BMC Biotechnology 2020 20:23

    The original article was published in BMC Biotechnology 2018 18:7

  4. In Norway, 3 million discarded egg-laying hens are destructed annually, which equals 1500 tons pure hen meat. Due to the slaughter methods used, this raw material is handled as a high-risk waste, while in real...

    Authors: Veronica Hjellnes, Rasa Šližyte, Turid Rustad, Ana Karina Carvajal and Kirsti Greiff
    Citation: BMC Biotechnology 2020 20:22
  5. This study investigated the feasibility of enhancing anaerobic digestion of sewage sludge with triple, dual, and individual pretreatment of waste activated sludge with heat, alkalinity, and hydrogen peroxide. ...

    Authors: Salar Siami, Behnoush Aminzadeh, Razieh Karimi and Seyed Mostafa Hallaji
    Citation: BMC Biotechnology 2020 20:21
  6. The coexistence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals has deleterious effects on environmental quality. Few reports have studied the mechanisms of plant inoculation with Piriformospora indic...

    Authors: Liang Li, Pengyue Zhu, Xiaoyang Wang and Zhenhua Zhang
    Citation: BMC Biotechnology 2020 20:20
  7. Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of ...

    Authors: Abbas Tanhaeian, Mehdi Mirzaii, Zana Pirkhezranian and Mohammad Hadi Sekhavati
    Citation: BMC Biotechnology 2020 20:19
  8. Contagious ecthyma or Orf is known as a zoonotic disease remains prevalently worldwide despite the application of some control strategies against it. RNAi particularly shRNA provides us with the chance to tack...

    Authors: Leila Asadi Samani, Behnaz Saffar, Azam Mokhtari and Ehsan Arefian
    Citation: BMC Biotechnology 2020 20:18
  9. Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially av...

    Authors: Szilvia Krisztina Nagy, Brigitta Margit Kállai, Judit András and Tamás Mészáros
    Citation: BMC Biotechnology 2020 20:17
  10. The type I human interferon (IFN) family consists of a group of cytokines with a multiplicity of biological activities, including antiviral, antitumor, and immunomodulatory effects. However, because the half-l...

    Authors: Qian Zhang, Chao Wang, Fenlian Ma, Lihong Yao, Hanchun Gao, Luyan Zhu and Lishu Zheng
    Citation: BMC Biotechnology 2020 20:16
  11. Infections caused by fungi are often refractory to conventional therapies and urgently require the development of novel options, such as immunotherapy. To produce therapeutic antibodies, a plant-based expressi...

    Authors: Marcello Catellani, Chiara Lico, Mauro Cerasi, Silvia Massa, Carla Bromuro, Antonella Torosantucci, Eugenio Benvenuto and Cristina Capodicasa
    Citation: BMC Biotechnology 2020 20:15
  12. Intercropping, an essential cultivation pattern in modern agricultural systems, increases crop yields and soil quality. Cassava and peanut intercropping systems exhibit advantages in solar utilization and cadm...

    Authors: Xiumei Tang, Ruichun Zhong, Jing Jiang, Liangqiong He, Zhipeng Huang, Guoying Shi, Haining Wu, Jing Liu, Faqian Xiong, Zhuqiang Han, Ronghua Tang and Longfei He
    Citation: BMC Biotechnology 2020 20:13
  13. Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant bioma...

    Authors: Leigh Gebbie, Tuan Tu Dam, Rebecca Ainscough, Robin Palfreyman, Li Cao, Mark Harrison, Ian O’Hara and Robert Speight
    Citation: BMC Biotechnology 2020 20:12
  14. As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in ...

    Authors: Jean-Michel Michno, Kamaldeep Virdi, Adrian O. Stec, Junqi Liu, Xiaobo Wang, Yer Xiong and Robert M. Stupar
    Citation: BMC Biotechnology 2020 20:10
  15. Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a...

    Authors: Bo Wang, Meifang Yu, Xianglin Zhu and Li Zhu
    Citation: BMC Biotechnology 2020 20:9
  16. The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternati...

    Authors: Tao He, Jörg Hausdorf, Yan Chevalier and Roland M. Klar
    Citation: BMC Biotechnology 2020 20:8
  17. Clostridium perfringens is the causative agent of several diseases and enteric infections in animals and humans. The virulence of C. perfringens is largely attributable to the production of numerous toxins; of th...

    Authors: Katia Forti, Monica Cagiola, Martina Pellegrini, Lucia Anzalone, Antonella Di Paolo, Sara Corneli, Giulio Severi and Antonio De Giuseppe
    Citation: BMC Biotechnology 2020 20:7
  18. The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH(1–110) from polyhedrin self-aggregates and incorporates foreign proteins to fo...

    Authors: Adolfo Cruz-Reséndiz, Jesús Zepeda-Cervantes, Alicia Sampieri, Carlos Bastián-Eugenio, Gonzalo Acero, J. Iván Sánchez-Betancourt, Goar Gevorkian and Luis Vaca
    Citation: BMC Biotechnology 2020 20:1
  19. Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools ...

    Authors: Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang and Weida Liu
    Citation: BMC Biotechnology 2020 20:6
  20. The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway. Several studies have demonstrated t...

    Authors: Gabriel Levin, Bruna Andrade Aguiar Koga, Gustavo Gross Belchior, Ana Claudia Oliveira Carreira and Mari Cleide Sogayar
    Citation: BMC Biotechnology 2020 20:5
  21. Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate...

    Authors: Yanqin Xu, Junjiang Zhou, Qingqing Liu, Kunpeng Li and Yin Zhou
    Citation: BMC Biotechnology 2020 20:4
  22. Insulin controls hyperglycemia caused by diabetes, and virtually all treatments require exogenous insulin. However, the product’s extensive post-translational modifications have hindered the manufacture of rec...

    Authors: Minmin Zhang, Yunlong Zhang, Bingnan Wu, Yanhao Peng, Altaf Ahmed Simair, Geoffery W. Siegel, Changrui Lu and Ting Chen
    Citation: BMC Biotechnology 2020 20:3
  23. Cellulosic biomass degradation still needs to be paid more attentions as bioenergy is the most likely to replace fossil energy in the future, and more evaluable cellulolytic bacteria isolation will lay a found...

    Authors: Lingling Ma, Yingying Lu, Hong Yan, Xin Wang, Yanglei Yi, Yuanyuan Shan, Bianfang Liu, Yuan Zhou and Xin Lü
    Citation: BMC Biotechnology 2020 20:2
  24. Interferon-gamma (IFN-γ) is an important mediator of type I immune response and has antiviral, immunoregulatory and anti-tumor properties, plays a wide range of roles in inflammation and autoimmune diseases. T...

    Authors: Wen-Tao Ma, Qi Liu, Meng-Xia Ning, Yu-Xu Qi, Saad Rehman and De-Kun Chen
    Citation: BMC Biotechnology 2019 19:102
  25. Clostridium perfringens, a gram-positive, anaerobic, rod-shaped bacterium, is the third leading cause of human foodborne bacterial disease and a cause of necrotic enteritis in poultry. It is controlled using anti...

    Authors: Rosemarie W. Hammond, Steven M. Swift, Juli A. Foster-Frey, Natalia Y. Kovalskaya and David M. Donovan
    Citation: BMC Biotechnology 2019 19:101
  26. Bacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates. These libraries typically express a scaffold protein embedded within ...

    Authors: Sarah D. Stellwagen, Deborah A. Sarkes, Bryn L. Adams, Mia A. Hunt, Rebecca L. Renberg, Margaret M. Hurley and Dimitra N. Stratis-Cullum
    Citation: BMC Biotechnology 2019 19:100
  27. To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality o...

    Authors: Min Zhang, Min Wei, Zhiguo Dong, Haibao Duan, Shuang Mao, Senlei Feng, Wenqian Li, Zepeng Sun, Jiawei Li, Kanglu Yan, Hao Liu, Xueping Meng and Hongxing Ge
    Citation: BMC Biotechnology 2019 19:99
  28. Wolbachia pipientis is a widespread, obligatory intracellular and maternally inherited bacterium, that induces a wide range of reproductive alterations to its hosts. Cytoplasmic Incompatibility (CI) is causing em...

    Authors: Georgios A. Kyritsis, Antonios A. Augustinos, Ioannis Livadaras, Carlos Cáceres, Kostas Bourtzis and Nikos T. Papadopoulos
    Citation: BMC Biotechnology 2019 19(Suppl 2):96

    This article is part of a Supplement: Volume 19 Supplement 2

  29. The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the S...

    Authors: Lucas Alexander Shuttleworth, Mohammed Abul Monjur Khan, Terrence Osborne, Damian Collins, Mukesh Srivastava and Olivia Louise Reynolds
    Citation: BMC Biotechnology 2019 19(Suppl 2):95

    This article is part of a Supplement: Volume 19 Supplement 2

  30. The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is an important polyphagous pest of horticultural produce. The sterile insect technique (SIT) is a proven control method against many i...

    Authors: Mahfuza Khan, Kajla Seheli, Md. Abdul Bari, Nahida Sultana, Shakil Ahmed Khan, Khandokar Fahmida Sultana and Md. Anwar Hossain
    Citation: BMC Biotechnology 2019 19(Suppl 2):94

    This article is part of a Supplement: Volume 19 Supplement 2

  31. The olive fly, Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research on B. oleae control methods, scientists ...

    Authors: Gaia Bigiotti, Roberta Pastorelli, Roberto Guidi, Antonio Belcari and Patrizia Sacchetti
    Citation: BMC Biotechnology 2019 19(Suppl 2):93

    This article is part of a Supplement: Volume 19 Supplement 2

  32. The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval devel...

    Authors: Doron Shalom Yishai Zaada, Michael Ben-Yosef, Boaz Yuval and Edouard Jurkevitch
    Citation: BMC Biotechnology 2019 19(Suppl 2):92

    This article is part of a Supplement: Volume 19 Supplement 2

  33. The symbiosis between the olive fruit fly, Bactrocera oleae, and Candidatus Erwinia dacicola has been demonstrated as essential for the fly’s larval development and adult physiology. The mass rearing of the olive...

    Authors: Patrizia Sacchetti, Roberta Pastorelli, Gaia Bigiotti, Roberto Guidi, Sara Ruschioni, Carlo Viti and Antonio Belcari
    Citation: BMC Biotechnology 2019 19(Suppl 2):91

    This article is part of a Supplement: Volume 19 Supplement 2

  34. Enterobacter sp. AA26 was recently isolated from the midgut of Ceratitis capitata (Wiedemann) and it was shown to have positive effects in rearing efficiency when used as larval probiotics. In this study, biomass...

    Authors: Konstantinos Azis, Ioanna Zerva, Paraschos Melidis, Carlos Caceres, Kostas Bourtzis and Spyridon Ntougias
    Citation: BMC Biotechnology 2019 19(Suppl 2):90

    This article is part of a Supplement: Volume 19 Supplement 2

  35. Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its ...

    Authors: Kala Bhandari, Peter Crisp and Michael A. Keller
    Citation: BMC Biotechnology 2019 19(Suppl 2):89

    This article is part of a Supplement: Volume 19 Supplement 2

  36. Symbiotic bacteria contribute to a multitude of important biological functions such as nutrition and reproduction and affect multiple physiological factors like fitness and longevity in their insect hosts. The...

    Authors: Elias D. Asimakis, Mahfuza Khan, Panagiota Stathopoulou, Carlos Caceres, Kostas Bourtzis and George Tsiamis
    Citation: BMC Biotechnology 2019 19(Suppl 2):88

    This article is part of a Supplement: Volume 19 Supplement 2

  37. Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, R...

    Authors: Wenchao Chen, Xuan Li, Xuli Ma, Shouwen Chen, Yanping Kang, Minmin Yang, Fenghong Huang and Xia Wan
    Citation: BMC Biotechnology 2019 19:98
  38. Immunogold labeling in combination with transmission electron microscopy analysis is a technique frequently used to correlate high-resolution morphology studies with detailed information regarding localization...

    Authors: Tohidul Islam, Anna L. Gharibyan, Cheng Choo Lee and Anders Olofsson
    Citation: BMC Biotechnology 2019 19:97
  39. The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of close...

    Authors: Xiaoyang Pang, Ziyang Jia, Jing Lu, Shuwen Zhang, Cai Zhang, Min Zhang and Jiaping Lv
    Citation: BMC Biotechnology 2019 19:87
  40. The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensiv...

    Authors: Jie Dong, Xiangzhen Ding and Sheng Wang
    Citation: BMC Biotechnology 2019 19:86
  41. The invasive fruit pest Drosophila suzukii was reported for the first time in Europe and the USA in 2008 and has spread since then. The adoption of type II clustered regularly interspaced short palindromic repeat...

    Authors: Hassan M. M. Ahmed, Luisa Hildebrand and Ernst A. Wimmer
    Citation: BMC Biotechnology 2019 19:85
  42. Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by ...

    Authors: Noah H. Richardson, Jordan B. Luttrell, Jonathan S. Bryant, Damian Chamberlain, Saleem Khawaja, Indira Neeli and Marko Radic
    Citation: BMC Biotechnology 2019 19:84
  43. The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are bein...

    Authors: Khadega A. Almaqdi, Rana Morsi, Bahia Alhayuti, Farah Alharthi and S. Salman Ashraf
    Citation: BMC Biotechnology 2019 19:83
  44. Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secret...

    Authors: Innanurdiani Koko, Adelene Ai-Lian Song, Mas Jaffri Masarudin and Raha Abdul Rahim
    Citation: BMC Biotechnology 2019 19:82
  45. Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ...

    Authors: Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans and Anne-Catherine Servais
    Citation: BMC Biotechnology 2019 19:81
  46. Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell...

    Authors: Yong Xu Mu, Yu Xia Zhao, Bing Yao Li, Hong Jing Bao, Hui Jiang, Xiao Lei Qi, Li Yun Bai, Yun Hong Wang, Zhi Jie Ma and Xiao Yun Wu
    Citation: BMC Biotechnology 2019 19:80
  47. Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p...

    Authors: Elinea O. Freitas, Bruno P. Melo, Isabela T. Lourenço-Tessutti, Fabrício B. M. Arraes, Regina M. Amorim, Maria E. Lisei-de-Sá, Julia A. Costa, Ana G. B. Leite, Muhammad Faheem, Márcio A. Ferreira, Carolina V. Morgante, Elizabeth P. B. Fontes and Maria F. Grossi-de-Sa
    Citation: BMC Biotechnology 2019 19:79

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