Articles
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Citation: BMC Biotechnology 2015 15:101
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Cloning and characterization of the pepper CaPAO gene for defense responses to salt-induced leaf senescence
Pheophorbide a oxygenase (PAO) is an important enzyme in the chlorophyll catabolism pathway and is involved in leaf senescence. It opens the porphyrin macrocycle of pheophorbide a and finally forms the primary fl...
Citation: BMC Biotechnology 2015 15:100 -
Defining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction
Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exq...
Citation: BMC Biotechnology 2015 15:99 -
Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones
Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. Th...
Citation: BMC Biotechnology 2015 15:98 -
Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11
Thermal stable α-glucosidases with transglycosylation activity could be applied to the industrial production of oligosaccharides as well as conjugation of sugars to biologically useful materials. Therefore, α-...
Citation: BMC Biotechnology 2015 15:97 -
A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis
Pullulanase is an important debranching enzyme and has been widely utilized to hydrolyse the α-1,6 glucosidic linkages in starch/sugar industry. Selecting new bacterial strains or improving bacterial strains i...
Citation: BMC Biotechnology 2015 15:96 -
Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells
Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine...
Citation: BMC Biotechnology 2015 15:95 -
A novel eurythermic and thermostale lipase LipM from Pseudomonas moraviensis M9 and its application in the partial hydrolysis of algal oil
Lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. Microbial lipases in particular have been widely used in a variety of industrial application...
Citation: BMC Biotechnology 2015 15:94 -
Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion
Practical approaches for multigene transformation and gene stacking are extremely important for engineering complex traits and adding new traits in transgenic crops. Trait deployment by gene stacking would gre...
Citation: BMC Biotechnology 2015 15:93 -
Expression screening using a Medaka cDNA library identifies evolutionarily conserved regulators of the p53/Mdm2 pathway
The p53 tumor suppressor protein is mainly regulated by alterations in the half-life of the protein, resulting in significant differences in p53 protein levels in cells. The major regulator of this process is ...
Citation: BMC Biotechnology 2015 15:92 -
Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia
RNA interference (RNAi) is an important molecular tool for analysis of gene function in vivo. Daphnia, a freshwater microcrustacean, is an emerging model organism for studying cellular and molecular processes inv...
Citation: BMC Biotechnology 2015 15:91 -
A new high-throughput screening-compatible gap junctional intercellular communication assay
Gap junctions (GJs) are intercellular channels through which molecules smaller than 1 kDa can diffuse, and they have been suggested as drug targets. To develop chemical drugs acting on this target, a high-thro...
Citation: BMC Biotechnology 2015 15:90 -
A Comparison of transgenic and wild type soybean seeds: analysis of transcriptome profiles using RNA-Seq
Soybean (Glycine max) has been bred for thousands of years to produce seeds rich in protein for human and animal consumption, making them an appealing bioreactor for producing valuable recombinant proteins at hig...
Citation: BMC Biotechnology 2015 15:89 -
Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris
Phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. Some phytases have been expressed in Pichia pastoris, among wh...
Citation: BMC Biotechnology 2015 15:88 -
Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells
Recombinant factor VII (rFVII), the precursor molecule for recombinant activated FVII (rFVIIa), is, due to its need for complex post translational modifications, produced in mammalian cells. To evaluate the su...
Citation: BMC Biotechnology 2015 15:87 -
Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain
Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six...
Citation: BMC Biotechnology 2015 15:86 -
Construction of polyomavirus-derived pseudotype virus-like particles displaying a functionally active neutralizing antibody against hepatitis B virus surface antigen
Virus-like particles (VLPs) can be efficiently produced by heterologous expression of viral structural proteins in yeast. Due to their repetitive structure, VLPs are extensively used for protein engineering an...
Citation: BMC Biotechnology 2015 15:85 -
High-level expression of a recombinant active microbial transglutaminase in Escherichia coli
Bacterial transglutaminases are increasingly required as industrial reagents for in vitro modification of proteins in different fields such as in food processing as well as for enzymatic site-specific covalent co...
Citation: BMC Biotechnology 2015 15:84 -
Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.
Citation: BMC Biotechnology 2015 15:83 -
The codon-optimized Δ6-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis
The ∆6-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications.
Citation: BMC Biotechnology 2015 15:82 -
Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed (Landoltia punctata)
Duckweed (Landoltia punctata) has the potential to remediate wastewater and accumulate enormous amounts of starch for bioethanol production. Using systematical screening, we determined that the highest biomass an...
Citation: BMC Biotechnology 2015 15:81 -
Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit v...
Citation: BMC Biotechnology 2015 15:80 -
An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin
A novel influenza virus, subtype H7N9, circulated through China in 2013–2014. Its higher rates of human infection in a wide range of locations within China and the associated increased likelihood of human-to-h...
Citation: BMC Biotechnology 2015 15:79 -
Cold shock induction of recombinant Arctic environmental genes
Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function...
Citation: BMC Biotechnology 2015 15:78 -
Immobilization of trypsin in organic and aqueous media for enzymatic peptide synthesis and hydrolysis reactions
Immobilization of enzymes onto different carriers increases enzyme’s stability and reusability within biotechnological and pharmaceutical applications. However, some immobilization techniques are associated wi...
Citation: BMC Biotechnology 2015 15:77 -
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices
In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrice...
Citation: BMC Biotechnology 2015 15:76 -
Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization
Transglutaminases (TGase), synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in food industry. Due to the important applications of TGase in food industry, obtaining robust and food-...
Citation: BMC Biotechnology 2015 15:75 -
Identification and characterization of laccase-type multicopper oxidases involved in dye-decolorization by the fungus Leptosphaerulina sp.
Fungal laccases are multicopper oxidases (MCOs) with high biotechnological potential due to their capability to oxidize a wide range of aromatic contaminants using oxygen from the air. Albeit the numerous lacc...
Citation: BMC Biotechnology 2015 15:74 -
Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
Fluorescence-enhancing microarray on plasmonic gold film is an attractive alternative to traditional enzyme-linked immunosorbent assay (ELISA) for cytokine detection because of the increased sensitivity. The a...
Citation: BMC Biotechnology 2015 15:73 -
The pigment characteristics and productivity shifting in high cell density culture of Monascus anka mycelia
Monascus mycelia and pigments are promising sources of food and medicine with their potential pharmaceutical values and health-improving functions. Using high cell density fermentation...
Citation: BMC Biotechnology 2015 15:72 -
Hybrid flagellin as a T cell independent vaccine scaffold
To extend the potency of vaccines against infectious diseases, vaccines should be able to exploit multiple arms of the immune system. One component of the immune system that is under-used in vaccine design is ...
Citation: BMC Biotechnology 2015 15:71 -
Challenges in the translation and commercialization of cell therapies
Cell therapies are an emerging form of healthcare that offer significant potential to improve the practice of medicine and provide benefits to patients who currently have limited or no treatment options. Ideal...
Citation: BMC Biotechnology 2015 15:70 -
Prokaryotic expression and mechanism of action of α-helical antimicrobial peptide A20L using fusion tags
Antimicrobial peptides have become important candidates as new antibiotics against resistant bacterial strains. However, the major industrial manufacture of antimicrobial peptides is chemical synthesis with hi...
Citation: BMC Biotechnology 2015 15:69 -
Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pat...
Citation: BMC Biotechnology 2015 15:68 -
Evaluation of Physarum polycephalum plasmodial growth and lipid production using rice bran as a carbon source
The myxomycete Physarum polycephalum appears to have remarkable potential as a lipid source for biodiesel production. The present study evaluated the use of rice bran as a carbon source and determined the medium ...
Citation: BMC Biotechnology 2015 15:67 -
High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine
L-ornithine (L-Orn), is an intermediate metabolite in the urea cycle that plays a significant role in humans. L-Orn can be obtained from the catalysis of L-arginine (L-Arg) by arginase. The Pichia pastoris expres...
Citation: BMC Biotechnology 2015 15:66 -
Evaluating whole transcriptome amplification for gene profiling experiments using RNA-Seq
RNA-Seq has enabled high-throughput gene expression profiling to provide insight into the functional link between genotype and phenotype. Low quantities of starting RNA can be a severe hindrance for studies th...
Citation: BMC Biotechnology 2015 15:65 -
Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci
RNA interference (RNAi) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsRNA/siRNA into target pests. Isaria fumosorosea is a common fungal p...
Citation: BMC Biotechnology 2015 15:64 -
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major det...
Citation: BMC Biotechnology 2015 15:63 -
Natural phenolics greatly increase flax (Linum usitatissimum) oil stability
Flaxseed oil is characterized by high content of essential polyunsaturated fatty acids (PUFA) promoted as a human dietary supplement protecting against atherosclerosis. The disadvantage of the high PUFA conten...
Citation: BMC Biotechnology 2015 15:62 -
Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells
The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to s...
Citation: BMC Biotechnology 2015 15:61 -
SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. We previously establishe...
Citation: BMC Biotechnology 2015 15:60 -
A delivery system for field application of paratransgenic control
As an alternative to chemical pesticides, paratransgenesis relies on transformation of symbiotic bacteria of an arthropod vector to deliver molecules that disrupt pathogen transmission. For over a decade parat...
Citation: BMC Biotechnology 2015 15:59 -
Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome
Human artificial chromosome (HAC) vectors have some unique characteristics as compared with conventional vectors, carrying large transgenes without size limitation, showing persistent expression of transgenes,...
Citation: BMC Biotechnology 2015 15:58 -
Structural differences of amyloid-β fibrils revealed by antibodies from phage display
Beside neurofibrillary tangles, amyloid plaques are the major histological hallmarks of Alzheimer’s disease (AD) being composed of aggregated fibrils of β-amyloid (Aβ). During the underlying fibrillogenic path...
Citation: BMC Biotechnology 2015 15:57 -
Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Expressing microbial polysaccharide-modifying enzymes in plants is an attractive approach to custom tailor plant lignocellulose and to study the importance of wall structures to plant development. Expression o...
Citation: BMC Biotechnology 2015 15:56 -
In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via 14C-TdR labeling
In order to shed light on the regenerative mechanism of mesenchymal stem cells (MSCs) in vivo, the bio-distribution profile of implanted cells using a stable and long-term tracking method is needed. We herein inv...
Citation: BMC Biotechnology 2015 15:55 -
High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
In our previous study, a novel liver-targeting fusion interferon (IFN-CSP) combining IFN α2b with plasmodium region I peptide was successfully constructed. IFN-CSP has significant inhibition effects on HBV-DNA re...
Citation: BMC Biotechnology 2015 15:54 -
A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli
Group 1 grass pollen allergens are a major cause of allergic disease. Specific immunotherapy involving controlled administration of allergens can be used as a disease-modifying treatment for such disease. Reco...
Citation: BMC Biotechnology 2015 15:52 -
Ghrelin accelerates the growth and osteogenic differentiation of rabbit mesenchymal stem cells through the ERK1/2 pathway
Mesenchymal stem cells (MSCs) can differentiate into chondroblasts, adipocytes, or osteoblasts under appropriate stimulation. Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor (GHSR), ...
Citation: BMC Biotechnology 2015 15:51
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