Skip to main content
  • Research article
  • Open access
  • Published:

Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production



Cyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical industries.


In this study, the cgt gene encoding α-CGTase from Paenibacillus macerans was codon-optimized (opt-cgt) and cloned into pET-28a (+) for intracellular expression in E. coli BL21 (DE3). The Opt-CGT was purified by Ni2+-NTA resin with a 55% recovery, and specific activity was increased significantly from 1.17 to 190.75 U·mg− 1. In addition, the enzyme was adopted to transform L-AA into 9.1 g/L of AA-2G. Finally, more economic substrates, including β-cyclodextrin, soluble starch, corn starch and cassava starch could also be used as glycosyl donors, and 4.9, 3.5, 1.3 and 1.5 g/L of AA-2G were obtained, respectively.


N-terminal amino acid is critical to the activity of CGTase suggested by its truncation study. Furthermore, when the Opt-CGT was flanked by His6-tags on the C- and N-terminal, the recovery of purification by Ni2+-NTA resin is appreciably enhanced. α-cyclodextrin was the ideal glycosyl donor for AA-2G production. In addition, the selection of low cost glycosyl donors would make the process of AA-2G production more economically competitive.


Ascorbic acid (Vitamin C, L-AA, Vc) is an essential vitamin in maintaining the health of body, which could not be synthesized naturally [1]. However, its hydroxyl group in the position of C-2 is extremely instable, especially in oxidative, lighted and hot environment, so the application of L-AA is subjected to a lot of restrictions [2]. 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) is a type of glucosylated L-AA, which is widely used in cosmetic, health products or other fields. In addition, it can play the role in whitening the skin owing to the L-AA released by α-glucosidase from AA-2G. Five kinds of enzymes could catalyze the AA-2G production, including α-amylase, α-glucosidase, sucrose phosphatase, α-isomaltosyl glucosaccharide-forming enzyme and cyclodextrin glycosyltransferase (EC, CGTase). Among them, CGTase with higher substrate specificity, higher production and more excellent thermal stability was considered to be the best enzyme for industrial production of AA-2G [3,4,5,6].

CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals [7]. Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10]. The CGTase from Bacillus genus belonging to glycoside hydrolases family 13 (GH13) is the most commonly used one, which can catalyze three kinds of reactions: cyclization, intramolecular transglycosylation and low hydrolytic reaction [11,12,13]. In addition, intramolecular transglycosylation reaction includes disproportionation reaction (transfer the donor substrate to acceptor substrate) and coupling reaction (cleave cyclodextrins and transfer the resulting maltooligosaccharide to acceptor substrate) [11].

The productivities of CGTases from wild strains are relatively low, partly resulting in high production costs for industrial applications. Currently, α-CGTases were mainly obtained by extracellular heterologous expression in E. coli or B. subtills. Compared with extracellular expression, intracellular expression would simplify the preservation, facilitate enzyme enrichment and isolate the disturbance of the external circumstances. In addition, typical yield of purified α-CG Tas was at a relatively low level by one-step affinity chromatography on Ni2+-NTA resin, and > 95% of the recombinant enzyme was left in the flow-through fraction because of the C-terminal His6-tag was partially inaccessible [14].

Previous reports showed that abundant biologically inactive inclusion bodies of α-CGTases accumulated when it was expressed intracellularly [14, 15]. Codon optimization is known to be an important strategy which could improve the levels of gene soluble expression in the host and ultimately affects enzyme production [16]. To enhance the α-CGTase soluble expression, the α-CGTase encoding gene from Paenibacillus macerans JFB05–01 was codon-optimized first, and then expressed in E. coli BL21(DE3) intracellularly. The recombinant Opt-CGT was flanked by His6-tags on the C- and N-terminal to increase binding affinity with Ni2+-NTA resin. The enzymatic properties were characterized and its ability of transforming L-AA to AA-2G was investigated. In addition, other more economic glycosyl donors were selected in order to decrease the cost of AA-2G production.


Chemicals and media

Peptone and yeast extract were purchased from Oxoid Co. Ltd. (Beijing, China), soluble starch was produced by Xilong chemical Co. Ltd. (Guangzhou, China), α-cyclodextrin was purchased from Sangon Biotech. Co. Ltd. (Shanghai, China) and the other chemical reagents were purchased from Lingfeng chemical reagents Co. Ltd. (Shanghai, China). All molecular biology reagents were purchased from TaKaRa Co., Ltd. (Dalian, China). All chemicals used in this study were of analytical grade or a higher purity. The Luria-Bertani (LB) medium was used to culture the E. coli strains in this study. The improved terrific broth (ITB) medium containing 15 g/L maltose, 12 g/L peptone, 24 g/L yeast extract, 16.43 g/L K2HPO4·3H2O and 2.31 g/L KH2PO4 was used for the fermentation of recombinant strain.

Strain, plasmid and primers

Secondary structure in dot-bracket notation with a minimum free energy (MFE) could be predicted using the ViennaRNA Web Services ( [17]. Codon adaptation index (CAI) based on the set of highly expressed genes from E. coli was calculated using the CAIcal server ( [18]. The mRNA senior structure and possible RNA stem structure were predicted by a web site of Finally, the α-CGTase encoding gene (cgt) from P. macerans JFB05–01 was codon-optimized by GeneWiz, Inc. (Suzhou, China) and the resulting gene was named as opt-cgt. The expression vector pET28a (+) with two His6-tags at both of C-terminal and N-terminal was used. The E. coli BL21 (DE3) was used as host strain and routinely grown aerobically at 37 °C in LB broth or on LB agar. All the primers used in the study were listed in Table 1.

Table 1 Oligonucleotide primers used for PCR

Cloning and expression of the opt-cgt gene encoding α-CGTase

The opt-cgt gene without signal peptide was amplified using the primers F1/R1 listed in Table 1 using PrimeSTAR HS DNA polymerase. Then, the PCR products were inserted into the BamH I and Xho I sites of the DNA pET28a (+) plasmid to produce the pET-opt-cgt plasmid by One Step Cloning Kit (Vazyme Biotech. Co., Ltd., Nanjing, China). The recombinant plasmid was transformed into E. coli BL21 (DE3) and designated as E. coli BL21 (DE3-pET-opt-cgt). Finally, the insertion of the genes was confirmed by DNA sequencing (GenScript Biotech. Co, Ltd., Nanjing, China).

E. coli BL21 (DE3-pET-opt-cgt) was incubated in 5 mL of LB medium supplemented with 30 μg/mL of kanamycin at 37 °C for 12 h as the inoculum. The 1.0 mL inoculum was inoculated into a 500 mL Erlenmeyer flask containing 100 mL ITB medium with 30 μg/mL kanamycin and then the condition was maintain at 200 r·min− 1 and 37 °C until the optical density at 600 nm (OD600) reached 0.5 to 0.6. Then, protein expression was induced with 0.01 mM IPTG and the culture temperature was lowered to 18 °C for 20 h. The bacterial cells were harvested by centrifugation (Eppendorf Centrifuge 5810 R, F-34-6-38 rotor, Brinkman Instruments Inc., USA) at 8085×g and 4 °C for 10 min, washed twice with 50 mM PBS (pH 6.0). The E. coli BL21 (DE3-pET-opt-cgt) cells were disrupted by a scientz-II D ultrasonic generator (Ningbo Scientz Biotech. co., Ltd., Ningbo, China) in the ice bath for 10 min. The cell debris was removed by centrifugation at 8085×g and 4 °C for 20 min.

In addition, the effects of Opt-CGT N-terminal amino acids on enzymatic activity were carried out by truncating expression. Four PCR fragments (cut off one amino acid, four amino acids, six amino acids and nine amino acids) were amplified using the primers F2/R2, F3/R3, F4/R4 and F5/R5, respectively (Table 1). Then, the four PCR fragments were inserted into pET28a (+) vector to produce the pET-cgt-t1AA, pET-cgt-t4AA, pET-cgt-t6AA and pET-cgt-t9AA plasmids which were transformed into E. coli BL21 (DE3) as described above.

Purification and SDS-PAGE

The recombinant Opt-CGT with His6-tags was purified with Ni2+-NTA resin (Qiagen, Valencia, CA, USA) [19]. After washing of non-target proteins with 25 mM imidazole in 50 mM PBS (pH 7.0), the target fusion protein was eluted with a linear concentration gradient of imidazole in 50 mM PBS (pH 7.0). The fractions containing target protein were collected and dialyzed against 50 mM PBS (pH 7.0) overnight at 4 °C to remove imidazole. The purified Opt-CGT and truncated proteins were diluted with buffer solution to 100 mL and stored at 4 °C, respectively.

The proteins were electrophoresed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and visualized by staining with Coomassie Brilliant Blue R-250 [20, 21]. SDS-PAGE was performed on a 12% gel using electrophoresis apparatus at 80 V for the first 60 min, followed by 120 V for 2 h.

Assay for cyclization activity of α-CGTase

α-Cyclodextrin activity was determined using the methyl orange method during starch hydrolysis [6]. The 0.1 mL of Opt-CGT was incubated in 0.9 mL of 50 mM PBS (pH 6.0) containing 3% (w/v) soluble starch at 40 °C for 10 min. Then, 1.0 mL of 1 M HCl was added to terminate the reaction and provided an acidic environment, and 1.0 mL of 0.1 mM methyl orange in 50 mM PBS (pH 6.0) was also added. Then the reaction mixture was incubated at 16 °C for 20 min and the amount of α-cyclodextrin in the mixture was spectrophotometrically determined by measuring the absorbance at 505 nm. The standard curve was calculated according to the OD505 of different concentration of pure AA-2G. One unit of α-cyclodextrin-forming activity was defined as the amount of enzyme that was able to produce 1 μmol of α-cyclodextrin per min.

Biochemical properties of the purified Opt-CGT

Effects of temperature and pH on Opt-CGT activity and stability

The optimal reaction pH was assessed at 40 °C using the following buffers: 50 mM citrate buffer, pH 4.0–6.0; 50 mM PBS, pH 6.0–8.0; 50 mM glycine-NaOH buffer, pH 8.0–10.0. The effect of temperature on Opt-CGT activity was determined under the optimal pH at different temperatures ranging from 16 to 80 °C. To measure pH stability, the enzyme was incubated at 4 °C for 1 h in different buffers and the residual activity was determined. The thermal stability of Opt-CGT was assessed by incubating the enzyme preparations at different temperatures for a certain time until the remaining activity decreased below 50% of its initial cyclization activity. Non-heated enzyme was used as the control (100%).

Effects of metal ions and chemical agents on Opt-CGT activity

Purified Opt-CGT was treated with 1 mM EDTA for 5 h at 4 °C and then dialyzed against 50 mM PBS (pH 6.0) to remove the EDTA. For reactivation, the metal-free enzyme was incubated with divalent metal ions (Fe2+, Ba2+, Cu2+, Co2+, Ni2+, Zn2+, Ca2+, Mg2+ and Mn2+) at a final concentration of 1 mM for 10 min, followed by the remaining activity determination. The procedure used to assess the metal ions was also used to determine the effects of chemical agents (Triton X-100, SDS, Tween20, Tween80 and urea) and organic reagents (methanol, ethanol, acetonitrile, isopropanol and DMSO) [22, 23]. The activity in the absence of any additives was used as the control (100%).

Assay for AA-2G synthesis

The original reaction mixture in 50 mM PBS (pH 5.0) contained 100 g/L of L-AA, 100 g/L of α-cyclodextrin and 60 U of Opt-CGT. The reaction mixture was incubated at 40 °C for 24 h in the dark and filled with nitrogen. In order to hydrolyze the AA-2-oligosaccharides (AA-2Gs) to AA-2G, the glucoamylase was added to the mixture at a final concentration of 10 U/mL. The reaction mixture was incubated at 55 °C for another 24 h. What’s more, the glycosyl donors were also optimized, and α-cyclodextrin, β-cyclodextrin, soluble starch, corn starch and cassava starch were all used as the glycosyl donors.

A separation column (internal diameter, 4.6 mm; length, 250 mm) filled with Kromasil 100–5-C18 was used for AA-2G HPLC analysis. The mobile phase was 25 mM KH2PO4: methanol (99.5:0.5, vol/vol) and adjusted pH to 2.0 using H3PO4. The flow rate was 0.8 mL/min and temperature of column was 25 °C. The detection wavelength was 238 nm. The retention time of the pure AA-2G was 13.034 min, and the amount of AA-2G was calculated on the basis of the peak area.

Results and discussion

Cloning and expression of the opt-cgt gene

The Opt-CGT was successfully expressed in E. coli BL21 (DE3) according to the results of SDS-PAGE and the analysis of activity. The activity of CGTase reached 6.25 U/mL after IPTG-induced 20 h. Generally, P. macerans should be incubated at 37 °C for 72 h to obtain the CGTase [24]. Hence, the fermentation time was shortened significantly by intracellular heterologous expression in E. coli comparing to that for P. macerans. In addition, the cgt gene was also cloned into pET-20b (+) and extracellularly expressed in E. coli with a higher enzyme activity of 22.5 U/mL and longer fermentation time of 90 h [14].

Codon bias was considered as the most important determinant of translation efficiency, expression level and the folding energy of mRNA [25]. The value of CAI increased from 0.74 to 0.86 for E. coli after the optimization of cgt gene. In theory, the CAI of 1.0 is considered as ideal, while a CAI of more than 0.8 is rated as good for expression in the desired expression organism [26]. The minimum free energy (ΔG) of the optimal secondary structure in dot-bracket notation was − 770.9 kcal·mol− 1 (1 cal = 4.2 J), while the ΔG of non-optimized codons was − 774.10 kcal·mol− 1 by ViennaRNA Web Services. Yin et al. reported that ΔG is closely related to translation efficiency and increasing ΔG can enhance the expression level [27]. Amaral et al. demonstrated that the folding free energy of the 5′ end of mRNA transcripts could have significant effects on translation efficiency and overall protein abundance [18]. Otherwise, the number of stable stem regions which has lower energy under − 15 kcal·mol− 1 was decreased from 15 to 10 after codon optimization. The lower energy was advantageous to soluble heterologous expression in E. coli [28].

Optimization of expression conditions and purification of Opt-CGT

In order to improve the enzyme activity of Opt-CGT, expression conditions were further optimized, including IPTG concentration and carbon sources. The optimized conditions for Opt-CGT expression was 200 r·min− 1 and 18 °C for 20 h with 0.01 mM of IPTG. The highest cyclization activity of Opt-CGT reached 11.12 U/mL when 15 g/L of maltose was used as the carbon source (Fig. 1 a, b and c). In general, intracellular expression of α-CGTase is more beneficial to the collection of enzyme. However, the enzyme activity is lower than that of extracellular expression. For example, α-cgt was cloned into pET-20b (+) and expressed in E. coli with the extracellular enzyme activity of 55.3 U/mL [29].

Fig. 1
figure 1

Condition optimization for Opt-CGT expression. a effects of different IPTG concentration; b effects of different carbon sources; c effects of maltose concentration on intracellular expression of Opt-CGT. Error bars correspond to the standard deviation of three measurements

The recombinant Opt-CGT was eluted by Ni2+-NTA resin and purified recombinant Opt-CGT was analyzed using SDS-PAGE (12%). The purified enzyme gave a single band on SDS-PAGE (Fig. 2). The molecular mass of the denatured enzyme was approximately 74 kDa, which was in good agreement with the theoretical molecular mass. After the Ni2+-NTA resin purification process, Opt-CGT was purified 163-fold with a 55% recovery and specific activity was increased significantly from 1.17 to 190.75 U·mg− 1. This is a similar specific activity with the reported two-step purification scheme (Q-sepharose and phenyl-superose), which the specific activity of purified recombinant α-CGTase was 198.82 U·mg− 1 [14] (Table 2). However, the recovery yield of purified enzyme reported by Li et al. was less than 5% by one-step affinity chromatography on Ni2+-NTA resin, which is a relatively low level. The increased yield of purified enzyme maybe because the plasmid of pET28a (+) flanked by His6-tags on the C- and N-terminal, which could increase Opt-CGT binding affinity with Ni2+-NTA resin.

Fig. 2
figure 2

SDS-PAGE analysis of the purified recombinant Opt-CGT. M protein marker; Lane 1 unpurified total soluble proteins from induced BL21 (DE3) harboring pET-opt-cgt; Lane 2–5 soluble proteins purified by nickel affinity chromatography from induced BL21 (DE3) harboring pET-opt-cgt (15 μL)

Table 2 Comparison of purification process between native CGTase and Opt-CGT

Effects of N-terminal amino acid on cyclization activity of Opt-CGT

The NH2 domain of CGTase was considered more important for cyclization [8]. In this study, 1 amino acid, 4 amino acids, 6 amino acids and 9 amino acids were truncated in the N-terminal of Opt-CGT, respectively. These truncated Opt-CGTs were designated CGT-t1AA, CGT-t4AA, CGT-t6AA and CGT-t9AA, which were expressed in E. coli BL21 (DE3), respectively. SDS-PAGE results indicated that all samples were highly expressed in E. coli (data not shown). However, the cyclization activity of CGT-t1AA was almost unchanged, and the activity of CGT-t4AA and CGT-t6AA was significantly reduced, whereas no activity could be detected by truncating 9 amino acids of Opt-CGT.

Salt bridges were important for the structure and function of enzymes, such as in the model metalloprotease PAE [30]. Based on the crystal structure of CGTase, two important salt bridges were found in the first 9 amino acids of CGTase at N-terminal, including Asp3-Arg521 and Lys9-Asp224. Three conserved amino acids (Asp229, Glu258 and Asp329) constituted the catalytic residues, which are important for cyclization activity of α-CGTase [31, 32]. Asp224 is closed to the Asp229 which is the active center of α-CGTase, thus, the destruction of the salt bridge Lys9-Asp224 may be the main reason to make Opt-CGT inactivation. The fact that the activity of CGT-t4AA and CGT-t6AA decreased but not completely lost maybe because Arg521 is relatively far from activity center of α-CGTase. This result could suggest the necessity of the N-terminal amino acids for Opt-CGT.

Biochemical properties of purified opt-CGT

Effects of temperature on opt-CGT activity and stability

In this study, the optimum temperature of the recombinant Opt-CGT was 50 °C for the cyclization activity (Fig. 3a). The recombinant enzyme exhibited more than 80% of maximum cyclization activity between 40 °C and 65 °C (Fig. 3a). Opt-CGT was stable and retained > 80% residual activity for 30 min at temperatures < 45 °C, but was unstable at temperatures > 50 °C (Fig. 3b). These results indicated that Opt-CGT was a mesophilic enzyme.

Fig. 3
figure 3

Effects of temperature and pH on enzyme activity and stability of the Opt-CGT. a determination of the optimum temperature; b thermal stability; c determination of the optimum pH; d pH stability. Error bars correspond to the standard deviation of three measurements

Effects of pH on Opt-CGT activity and stability

Opt-CGT exerted high levels of activity at pH 5.0–6.5 with an optimum pH of 6.0 (Fig. 3c). Almost no activity was detected at pH below 4.0 or above 9.5. The recombinant Opt-CGT was more active in glycine-NaOH buffer compared with that in PBS buffer at the same pH. The stabilities of recombinant Opt-CGT for the cyclization activity was also examined in the pH range of 4.0–12.0 (Fig. 3d). Opt-CGT retained > 70% of its activity after storing at pH 5.0–11.5 for 1 h, whereas its stabilities decreased significantly at pHs below pH 5.0 or above pH 11.5 [14].

Effects of metal ions and chemical agents on Opt-CGT activity

To determine whether metal ions would affect the cyclization activity, the residual activity of metal-free enzyme was assayed by incubation with 1 mM of divalent metal ions for 10 min. As shown in Tables 3, 1 mM of EDTA almost did not inhibit the enzymatic activity, indicating that the Opt-CGT represented a divalent metal ion non-dependent glycosyl transferase. Zn2+, Co2+, Mn2+and Mg2+ ions had little effects on the activity, whereas Fe2+, Cu2+ and Ni2+ inhibited the activity at a final concentration of 1 mM. On the other hand, 1 mM of Ca2+ or Ba2+ activated the cyclization activity of Opt-CGT up to 114.9 and 111.7%, respectively. The effects of metal ions and the metal chelator on the recombinant Opt-CGT and non-optimized CGT were almost similar [14].

Table 3 Effects of bivalent metal ions and chemical agents on Opt-CGT activity

The effects of various chemical agents on the enzyme activity were also assessed (Table 3). Methanol, ethanol, acetonitrile, isopropanol, Tween 20, Tween 80 and SDS strongly inhibited Opt-CGT enzyme activity, whereas DMSO and Triton X-100 slightly reduced its activity. Urea had no significant influence on the enzyme activity. These data suggested that Opt-CGT was a metal ion non-dependent enzyme and its organic solvent tolerance is poor.

Overall, the biochemical characteristics are almost the same between Opt-CGT and native CGTase (Table 4). However, the half-life of the native enzyme at 50 °C is 48 min, whereas the Opt-CGT was just about 20 min, indicating that the native CGTase has a better thermal tolerance. Interestingly, there was some improvement in the pH stability of Opt-CGT, suggesting that the Opt-CGT still maintained an excellent stability at the pH 11.0.

Table 4 Comparison of the properties between native CGTase and Opt-CGT

Production of AA-2G

Many oligosaccharides and polysaccharides could be used as substrates for the transformation of AA-2G. Usually, α-CD was the ideal glycosyl donor for AA-2G production. The highest concentration of AA-2G reached 9.1 g/L when the temperature, pH, amount of enzyme, concentration of L-AA and α-CD were 40 °C, 5.0, 60 U, 100 g/L and 100 g/L, respectively (Fig. 4). Jun et al. reported that AA-2G production concentration was 1.53 g/L using 30 g/L of AA, 70 g/L of glycosyl donor and 2000 U/mL of CGTase in the catalytic system, which was much lower than this study [4].

Fig. 4
figure 4

HPLC analysis of AA-2G production by the Opt-CGT. a the standard sample of L-AA (0.1 g/L); b the standard sample of AA-2G (0.1 g/L); c reaction products obtained: The peak I is the rest of L-AA and the peak II is the generated AA-2G. The reaction sample was diluted 1000 times before analysis

In addition, due to the relatively high price of α-CD, a lot of other glycosyl donors were selected, such as β-CD, soluble, corn and cassava starch. When β-cyclodextrin was used as the glycosyl donor, another cyclodextrin composed of seven glucose molecules, 4.9 g/L of AA-2G could be obtained, and the decreased production maybe because the lower solubility of β-cyclodextrin. However, the cost of β-cyclodextrin is still expensive for industrial production. To further decrease the cost, starches were thought to act as the glycosyl donors, and the production of AA-2G reached 3.5 , 1.3 and 1.5 g/L from soluble, corn and cassava starch, respectively. Compared to the price of different starches, corn and cassava starch are more economic than soluble starch. However, when different starches were used as the glycosyl donors, the production of AA-2G was still at a relatively low level. Hence, future study should be performed to improve the AA-2G production from economic glycosyl donor.


The α-CGTase encoding gene from P. macerans JFB05–01 was successfully codon-optimized and expressed intracellularly in E. coli BL21 (DE3) . The effects of N-terminal amino acids on activity of CGTase were also confirmed by truncation experiment. Then the recombinant enzyme was purified with a 55% yield by Ni2+-NTA resin and characterized its biochemical properties. In order to improve the production of AA-2G, the catalytic conditions are optimized based on the characterizations of purified Opt-CGT. In addition, considering the relatively high cost of cyclodextrin, the glycosyl donor could be changed to starches, but the production should be further improved.


  1. Jacob RA, Sotoudeh G. Vitamin C function and status in chronic disease. Nutr Clin Care. 2010;5(2):66–74.

    Article  Google Scholar 

  2. Yamamoto I, Tai A. The current state on development of novel vitamin derivatives. Nihon Rinsho. 1999;57(10):2332–8.

    CAS  PubMed  Google Scholar 

  3. Han R, Liu L, Li J, Du G, Chen J. Functions, applications and production of 2-O-D-glucopyranosyl-L-ascorbic acid. Appl Microbiol Biotechnol. 2012;95(2):313–20.

    Article  CAS  PubMed  Google Scholar 

  4. Jun HK, Bae KM, Kim SK. Production of 2-O-α-D-glucopyranosyl L-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp. Biotechnol Lett. 2001;23(21):1793–7.

    Article  CAS  Google Scholar 

  5. Tanaka M, Muto N, Yamamoto I. Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-α-glucoside formation. Biochim Biophys Acta. 1991;1078(2):127–32.

    Article  CAS  PubMed  Google Scholar 

  6. Zhang Z, Li J, Liu L, Sun J, Hua Z, Du G, Chen J. Enzymatic transformation of 2-O-α-D-glucopyranosyl-L-ascorbic acid by α-cyclodextrin glucanotransferase from recombinant Escherichia coli. Biotechnol Bioprocess Eng. 2011;16(1):107–13.

    Article  CAS  Google Scholar 

  7. Ng HS, Ooi CW, Mokhtar MN, Show PL, Ariff A, Tan JS, Ng EP, Ling TC. Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system. Bioresour Technol. 2013;142:723–6.

    Article  CAS  PubMed  Google Scholar 

  8. Fujiwara S, Kakihara H, Woo KB, Lejeune A, Kanemoto M, Sakaguchi K, Imanaka T. Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme. Appl Environ Microbiol. 1992;58(12):4016–25.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Rather MY, Ara KZG, Nordberg Karlsson E, Adlercreutz P. Characterization of cyclodextrin glycosyltransferases (CGTases) and their application for synthesis of alkyl glycosides with oligomeric head group. Process Biochem. 2015;50(5):722–8.

    Article  CAS  Google Scholar 

  10. Ong RM, Goh KM, Mahadi NM, Hassan O, Rahman RN, Illias RM. Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp. G1 in E. coli. J Ind Microbiol Biotechnol. 2008;35(12):1705–14.

    Article  CAS  PubMed  Google Scholar 

  11. Fenelon VC, Aguiar MF, Miyoshi JH, Martinez CO, Matioli G. Ultrafiltration system for cyclodextrin production in repetitive batches by CGTase from Bacillus firmus strain 37. Bioprocess Biosyst Eng. 2015;38(7):1291–301.

    Article  CAS  PubMed  Google Scholar 

  12. Mathew S, Adlercreutz P. Cyclodextrin glucanotransferase (CGTase) catalyzed synthesis of dodecyl glucooligosides by transglycosylation using α-cyclodextrin or starch. Carbohydr Polym. 2012;87(1):574–80.

    Article  CAS  PubMed  Google Scholar 

  13. Yong J, Jin NC, Park SS, Park CS, Park KH, Yang DC. Secretion of heterologous cyclodextrin glycosyltransferase of Bacillus sp. E1 from Escherichia coli. Biotechnol Lett. 1996;18(10):1223–8.

    Article  CAS  Google Scholar 

  14. Li Z, Li B, Gu Z, Du G, Wu J, Chen J. Extracellular expression and biochemical characterization of alpha-cyclodextrin glycosyltransferase from Paenibacillus macerans. Carbohydr Res. 2010;345(7):886–92.

    Article  CAS  PubMed  Google Scholar 

  15. Kim SG, Kweon DH, Lee DH, Park YC, Seo JH. Coexpression of folding accessory proteins for production of active cyclodextrin glycosyltransferase of Bacillus macerans in recombinant Escherichia coli. Protein Expr Purif. 2005;41(2):426–32.

    Article  CAS  PubMed  Google Scholar 

  16. Park S, Chang KS, Jin E, Pack SP, Lee J. Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina. Bioprocess Biosyst Eng. 2013;36(1):127–31.

    Article  CAS  PubMed  Google Scholar 

  17. Gruber AR, Lorenz R, Bernhart SH, Neubock R, Hofacker IL. The Vienna RNA websuite. Nucleic Acids Res. 2008;36(Web Server):W70–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Amaral FE, Parker D, Randis TM, Kulkarni R, Prince AS, Shirasu-Hiza MM, Ratner AJ. Rational manipulation of mRNA folding free energy allows rheostat control of pneumolysin production by Streptococcus pneumoniae. PLoS One. 2015;10(3):e0119823.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Janknecht R, Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–5.

    Article  CAS  PubMed  Google Scholar 

  21. Li G, Jiang Y, Fan XJ, Liu YH. Molecular cloning and characterization of a novel beta-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomic library. Bioresour Technol. 2012;123:15–22.

    Article  CAS  PubMed  Google Scholar 

  22. Sinha R, Khare SK. Characterization of detergent compatible protease of a halophilic Bacillus sp. EMB9: differential role of metal ions in stability and activity. Bioresour Technol. 2013;145:357–61.

    Article  CAS  PubMed  Google Scholar 

  23. Dong W, Jiang S, Shi K, Wang F, Li S, Zhou J, Huang F, Wang Y, Zheng Y, Hou Y, Huang Y, Cui Z. Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter sp. strain DL-2 and cloning of FE hydrolase gene afeH. Bioresour Technol. 2015;186:114–21.

    Article  CAS  PubMed  Google Scholar 

  24. Abdel-Naby MA. Immobilization of Paenibacillus macerans NRRL B-3186 cyclodextrin glucosyltransferase and properties of the immobilized enzyme. Process Biochem. 1999;34(4):399–405.

    Article  CAS  Google Scholar 

  25. Tuller T, Waldman YY, Kupiec M, Ruppin E. Translation efficiency is determined by both codon bias and folding energy. Proc Natl Acad Sci U S A. 2010;107(8):3645–50.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Darabi P, Galehdari H, Khatami SR, Shahbazian N, Shafeei M, Jalali A, Khodadadi A. Codon optimization, cloning and expression of the human leukemia inhibitory factor (hLIF) in E. Iran J Biotechnol. 2013;11(1):47–53.

    Article  CAS  Google Scholar 

  27. Yin J, Bao L, Tian H, Gao X, Yao W. Quantitative relationship between the mRNA secondary structure of translational initiation region and the expression level of heterologous protein in Escherichia coli. J Ind Microbiol Biotechnol. 2016;43(1):97–102.

    Article  CAS  PubMed  Google Scholar 

  28. Su N, Wu JJ, Li Y, Zhang C, Li M, Xing XH. Construction of MBP fusion heparinaseIII efficient expression system in E.coil. CIESC J. 2014;65(7):14.

    Google Scholar 

  29. Liu H, Li J, Du G, Zhou J, Chen J. Enhanced production of alpha-cyclodextrin glycosyltransferase in Escherichia coli by systematic codon usage optimization. J Ind Microbiol Biotechnol. 2012;39(12):1841–9.

    Article  CAS  PubMed  Google Scholar 

  30. Bian F, Yue S, Peng Z, Zhang X, Chen G, Yu J, Xuan N, Bi Y. A comprehensive alanine-scanning mutagenesis study reveals roles for salt bridges in the structure and activity of Pseudomonas aeruginosa elastase. PLoS One. 2015;10(3):e0121108.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Han R, Li J, Shin HD, Chen RR, Du G, Liu L, Chen J. Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications. Biotechnol Adv. 2014;32(2):415–28.

    Article  CAS  PubMed  Google Scholar 

  32. Veen BA, van Alebeek GJ, Uitdehaag JC, Dijkstra BW, Dijkhuizen L. The three transglycosylation reactions catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans (strain 251) proceed via different kinetic mechanisms. Eur J Biochem. 2000;267(3):658–65.

    Article  PubMed  Google Scholar 

Download references


This work was supported by the National Natural Science Foundation of China (Nos. 31700092, 21706125, 21727818, 21706124), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_1109), the Jiangsu Province Natural Science Foundation for Youths (Nos. BK20170993, BK20170997), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1834), Project of State Key Laboratory of Materials-Oriented Chemical Engineering (KL17–09).

Availability of data and materials

The datasets supporting the conclusions of this article are included within the article.

Author information

Authors and Affiliations



JM and DWL designed the experiments; JYJ, ZJ, WRF, XFX and ZWM performed the experiments; FY, MJF, ZJ, WRF and DWL analyzed the data; JYJ and DWL wrote the paper; JM revised the paper. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Weiliang Dong or Min Jiang.

Ethics declarations

Ethics approval and consent to participate

Not applicable

Consent for publication

Not applicable

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiang, Y., Zhou, J., Wu, R. et al. Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production. BMC Biotechnol 18, 53 (2018).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: