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Table 4 Comparison of the properties between native CGTase and Opt-CGT

From: Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

Details

Opt-CGT (This study)

Native CGTase

Induction time (h)

20

70

Molecular mass

74 kDa

75 kDa

Optimum temperature (°C)

50

50

Half-life time of the CGTases at 50 °C (min)

~ 20

~ 48

Optimum pH

6.0

5.5

pH stabilities (The activity of CGTases retained > 80%)

6.0–11.5

6.0–10.0