Additives | Concentration | Relative activity (%) |
---|---|---|
Metal ions | ||
No addition | 100 ± 1.8 | |
Fe2+(FeCl2) | 1 mM | 80.6 ± 3.5 |
Mg2+(MgCl2) | 1 mM | 96.6 ± 1.9 |
Cu2+(CuCl2) | 1 mM | 59.9 ± 4.8 |
Ca2+(CaCl2) | 1 mM | 114.9 ± 2.4 |
Ni2+(NiCl2) | 1 mM | 79.3 ± 3.9 |
Zn2+(ZnCl2) | 1 mM | 97.3 ± 2.7 |
Ba2+(BaCl2) | 1 mM | 111.7 ± 2.2 |
Mn2+(MnCl2) | 1 mM | 101.8 ± 3.1 |
Co2+(CoCl2) | 1 mM | 94.1 ± 2.3 |
EDTA | 1 mM | 92.5 ± 2.8 |
Organic solvents | ||
Methanol | 10% | 36.9 ± 3.2 |
Ethanol | 10% | 13.8 ± 1.2 |
Acetonitrile | 10% | 5.5 ± 0.9 |
Isopropanol | 10% | 18.9 ± 2.8 |
DMSO | 5% | 74.2 ± 2.3 |
Surfactants | ||
Tween-20 | 20 mg·mL−1 | 32.4 ± 3.1 |
Tween-80 | 20 mg·mL− 1 | 55.8 ± 3.7 |
Triton X-100 | 20 mg·mL−1 | 87.1 ± 2.4 |
SDS | 2 mg·mL−1 | 36.9 ± 3.1 |
Enzyme inhibitors | ||
Urea | 20 mg·mL−1 | 97.6 ± 2.7 |