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Table 3 Effects of bivalent metal ions and chemical agents on Opt-CGT activity

From: Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

Additives

Concentration

Relative activity (%)

Metal ions

 No addition

 

100 ± 1.8

 Fe2+(FeCl2)

1 mM

80.6 ± 3.5

 Mg2+(MgCl2)

1 mM

96.6 ± 1.9

 Cu2+(CuCl2)

1 mM

59.9 ± 4.8

 Ca2+(CaCl2)

1 mM

114.9 ± 2.4

 Ni2+(NiCl2)

1 mM

79.3 ± 3.9

 Zn2+(ZnCl2)

1 mM

97.3 ± 2.7

 Ba2+(BaCl2)

1 mM

111.7 ± 2.2

 Mn2+(MnCl2)

1 mM

101.8 ± 3.1

 Co2+(CoCl2)

1 mM

94.1 ± 2.3

 EDTA

1 mM

92.5 ± 2.8

Organic solvents

 Methanol

10%

36.9 ± 3.2

 Ethanol

10%

13.8 ± 1.2

 Acetonitrile

10%

5.5 ± 0.9

 Isopropanol

10%

18.9 ± 2.8

 DMSO

5%

74.2 ± 2.3

Surfactants

 Tween-20

20 mg·mL−1

32.4 ± 3.1

 Tween-80

20 mg·mL− 1

55.8 ± 3.7

 Triton X-100

20 mg·mL−1

87.1 ± 2.4

 SDS

2 mg·mL−1

36.9 ± 3.1

Enzyme inhibitors

 Urea

20 mg·mL−1

97.6 ± 2.7