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Table 3 Effects of bivalent metal ions and chemical agents on Opt-CGT activity

From: Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

Additives Concentration Relative activity (%)
Metal ions
 No addition   100 ± 1.8
 Fe2+(FeCl2) 1 mM 80.6 ± 3.5
 Mg2+(MgCl2) 1 mM 96.6 ± 1.9
 Cu2+(CuCl2) 1 mM 59.9 ± 4.8
 Ca2+(CaCl2) 1 mM 114.9 ± 2.4
 Ni2+(NiCl2) 1 mM 79.3 ± 3.9
 Zn2+(ZnCl2) 1 mM 97.3 ± 2.7
 Ba2+(BaCl2) 1 mM 111.7 ± 2.2
 Mn2+(MnCl2) 1 mM 101.8 ± 3.1
 Co2+(CoCl2) 1 mM 94.1 ± 2.3
 EDTA 1 mM 92.5 ± 2.8
Organic solvents
 Methanol 10% 36.9 ± 3.2
 Ethanol 10% 13.8 ± 1.2
 Acetonitrile 10% 5.5 ± 0.9
 Isopropanol 10% 18.9 ± 2.8
 DMSO 5% 74.2 ± 2.3
Surfactants
 Tween-20 20 mg·mL−1 32.4 ± 3.1
 Tween-80 20 mg·mL− 1 55.8 ± 3.7
 Triton X-100 20 mg·mL−1 87.1 ± 2.4
 SDS 2 mg·mL−1 36.9 ± 3.1
Enzyme inhibitors
 Urea 20 mg·mL−1 97.6 ± 2.7