Fig. 2From: Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid productionSDS-PAGE analysis of the purified recombinant Opt-CGT. M protein marker; Lane 1 unpurified total soluble proteins from induced BL21 (DE3) harboring pET-opt-cgt; Lane 2–5 soluble proteins purified by nickel affinity chromatography from induced BL21 (DE3) harboring pET-opt-cgt (15 μL)Back to article page