Table 2 Comparison of purification process between native CGTase and Opt-CGT
Details | Opt-CGT (This study) | Native CGTase |
|---|---|---|
Origin Organism | Paenibacillus macerans JFB05–01 | Paenibacillus macerans JFB05–01 |
Codon optimization | Yes | No |
CAI (E. coli) | 0.86 | 0.74 |
Plasmid/host | pET-28a (+)/E. coli BL21 | pET-20b (+)/E. coli BL21 |
Induction time (h) | 20 | 90 |
Total protein of crude enzyme (mg) | 330 | 401 |
Purification methods | Ni2+-NTA | Q-Sepharose + Phenyl-Superose |
Total protein of purified enzyme (mg) | 7.66 | 8.31 |
Specific activity (U/mg) | 190.75 | 198.82 |
Purification fold | 163 | 13.17 |
Yield (%) | 55 | 27.27 |