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Table 2 Comparison of purification process between native CGTase and Opt-CGT

From: Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

Details Opt-CGT (This study) Native CGTase
Origin Organism Paenibacillus macerans JFB05–01 Paenibacillus macerans JFB05–01
Codon optimization Yes No
CAI (E. coli) 0.86 0.74
Plasmid/host pET-28a (+)/E. coli BL21 pET-20b (+)/E. coli BL21
Induction time (h) 20 90
Total protein of crude enzyme (mg) 330 401
Purification methods Ni2+-NTA Q-Sepharose + Phenyl-Superose
Total protein of purified enzyme (mg) 7.66 8.31
Specific activity (U/mg) 190.75 198.82
Purification fold 163 13.17
Yield (%) 55 27.27