Abel S, Theologis A. Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant J. 1994;5:421–7.
Article
CAS
Google Scholar
Faraco M, Di Sansebastiano GP, Spelt K, Koes RE, Quattrocchio FM. One protoplast is not the other! Plant Physiol. 2011;156:474–8.
Article
CAS
Google Scholar
Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007;2:1565–72.
Article
CAS
Google Scholar
Sheen J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 2001;127:1466–75.
Article
CAS
Google Scholar
Siritunga D, Arias-Garzon D, White W, Sayre R. Over-expression of hydroxynitrile lyase in transgenic cassava (Manihot esculenta Crantz) roots accelerates cyanogenesis. Plant Biotech J. 2004;2:37–43.
Article
CAS
Google Scholar
Miao YS, Jiang LW. Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. Nat Protoc. 2007;2:2348–53.
Article
CAS
Google Scholar
De Caroli M, Lenucci MS, Manualdi F, Dalessandro G, De Lorenzo G, Piro G. Molecular dissection of Phaseolus vulgaris polygalacturonase-inhibiting protein 2 reveals the presence of hold/release domains affecting protein trafficking toward the cell wall. Front Plant Sci. 2015;6:660.
Article
Google Scholar
Nanjareddy K, Arthikala MK, Blanco L, Arellano ES, Lara M. Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis. BMC Biotechnol. 2016;16:53.
Article
Google Scholar
Chen SB, Tao LZ, Zeng LR, Vega-Sanchez M, Umemura KJ, Wang GL. A highly efficient transient protoplast system for analyzing defence gene expression and protein-protein interactions in rice. Mol Plant Pathol. 2006;7:417–27.
Article
CAS
Google Scholar
Kim MJ, Baek K, Park CM. Optimization of conditions for transient Agrobacterium-mediated gene expression assays in Arabidopsis. Plant Cell Rep. 2009;28:1159–67.
Article
CAS
Google Scholar
Walter M, Chaban C, Schütze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J. 2004;40:428–38.
Article
CAS
Google Scholar
Guo J, Morrell-Falvey JL, Labbe JL, Muchero W, Kalluri UC, Tuskan GA, Chen J-G. Highly Efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. PLoS One. 2012;7, e44908.
Article
CAS
Google Scholar
Guo Y, Song X, Zhao S, Lv J, Lu M. A transient gene expression system in Populus euphratica Oliv. protoplasts prepared from suspension cultured cells. Acta Physiol Plant. 2015;37:160.
Article
Google Scholar
Tan BY, Xu M, Chen Y, Huang MR. Transient expression for functional gene analysis using Populus protoplasts. Plant Cell Tissue Org Cult. 2013;114:11–8.
Article
CAS
Google Scholar
Huang H, Wang Z, Cheng J, Zhao W, Li X, Wang H, Zhang Z, Sui X. An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system. Sci Hortic. 2013;150:206–12.
Article
CAS
Google Scholar
Bai Y, Han N, Wu J, Yang Y, Wang J, Zhu M, Bian H. A transient gene expression system using barley protoplasts to evaluate microRNAs for post-transcriptional regulation of their target genes. Plant Cell Tiss Organ Cult. 2014;119:211–9.
Article
CAS
Google Scholar
Wang H, Wang W, Zhan J, Huang W, Xu H. An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes. Sci Hortic. 2015;191:82–9.
Article
CAS
Google Scholar
Burris KP, Dlugosz EM, Collins AG, Stewart Jr CN, Lenaghan SC. Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.). Plant Cell Rep. 2016;35:693–704.
Article
CAS
Google Scholar
Hong SY, Seo PJ, Cho SH, Park CM. Preparation of leaf mesophyll protoplasts for transient gene expression in Brachypodium distachyon. J Plant Biol. 2012;55:390–7.
Article
CAS
Google Scholar
Shen J, Fu J, Ma J, Wang X, Gao C, Zhuang C, Wan J, Jiang L. Isolation, culture, and transient transformation of plant protoplasts. Curr Protoc Cell Biol. 2014;63:2.8.1–17.
Article
Google Scholar
Alves AGC. Cassava botany and physiology. In: Hillocks RJ, Thresh JM, Bellotti AC, editors. Cassava biology, production and utilization. Wallingford: CABI; 2001. p. 67–90.
Google Scholar
Jennings DL, Iglesias C. Breeding for crop improvement. In: Hillocks RJ, Thresh JM, Bellotti AC, editors. Cassava biology, production and utilization. Wallingford, CABI/Embrapa, Colombia. 2001. p. 149-65.
Liu J, Zheng Q, Ma Q, Gadidasu KK, Zhang P. Cassava genetic transformation and its application in breeding. J Integr Plant Biol. 2011;53:552–69.
Article
CAS
Google Scholar
Prochnik S, Marri PR, Desany B, Rabinowicz PD, Kodira C, Mohiuddin M, Rodriguez F, Fauquet C, Tohme J, Harkins T, Rokhsar DS, Rounsley S. The cassava genome: current progress, future directions. Trop Plant Biol. 2012;5:88–94.
Article
CAS
Google Scholar
Zhang P, Legris G, Coulin P, Puonti-Kaerlas J. Production of stably transformed cassava plants via particle bombardment. Plant Cell Rep. 2000;19:939–45.
Article
CAS
Google Scholar
Siritunga D, Sayre R. Generation of cyanogen-free transgenic cassava. Planta. 2003;217:367–73.
Article
CAS
Google Scholar
Siritunga D, Sayre R. Engineering cyanogens in cassava. Plant Mol Biol. 2004;56:661–9.
Article
CAS
Google Scholar
Zhang P, Puonti-Kaerlas J. PEG-mediated cassava transformation using positive and negative selection. Plant Cell Rep. 2000;19:1041–48.
Article
CAS
Google Scholar
Nyaboga EN, Njiru JM, Tripathi L. Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14. Front Plant Sci. 2015;6:411.
Article
Google Scholar
Zainuddin IM, Schlegel K, Gruissem W, Vanderschuren H. Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces. Plant Methods. 2012;8:24.
Article
Google Scholar
Shahin EA, Shephard JF. Cassava mesophyll protoplasts: Isolation, proliferation, and shoot formation. Plant Sci Lett. 1980;17:459–65.
Article
CAS
Google Scholar
Sofiari E, Raemakers CJJM, Bergervoet JEM, Jacobsen E, Visser RGF. Plant regeneration from protoplasts isolated from friable embryogenic callus of cassava. Plant Cell Rep. 1998;18:159–65.
Article
CAS
Google Scholar
Anthony P, Davey MR, Power JB, Lowe KC. An improved protocol for the culture of cassava leaf protoplasts. Plant Cell Tiss Organ Cult. 1995;42:299–302.
Article
CAS
Google Scholar
De Angeli A, Monachello D, Ephritikhine G, Frachisse JM, Thomine S, Gambale F, Barbier-Brygoo H. The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles. Nature. 2006;442:939–42.
Article
Google Scholar
Kantharajah A, Dodd W. Factors that influence the yield and viability of cucumber (Cucumis sativus L.) cotyledon protoplasts. Aust J Bot. 1990;38:169–75.
Article
Google Scholar
Davey MR, Anthony P, Power JB, Lowe KC. Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv. 2005;23:131–71.
Article
CAS
Google Scholar
Zhu L, Wang B, Zhou J, Chen L, Dai C, Duan C. Protoplast isolation of callus in Echinacea augustifolia. Colloid Surface B. 2005;44:1–5.
Article
CAS
Google Scholar
Raikar SV, Braun RH, Bryant C, Conner AJ, Christey MC. Efficient isolation, culture and regeneration of Lotus corniculatus protoplasts. Plant Biotechnol Rep. 2008;2:171–7.
Article
Google Scholar
Cordoba E, Aceves-Zamudio DL, Hernández-Bernal AF, Ramos-Vega M, León P. Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana. J Exp Bot. 2015;66:147–59.
Article
CAS
Google Scholar
Sauer N, Friedländer K, Gräml-Wicke U. Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. EMBO J. 1990;9:3045–50.
CAS
Google Scholar
Sauer N, Stadler R. A sink-specific H+/monosaccharide co-transporter from Nicotiana tabacum: cloning and heterologous expression in baker's yeast. Plant J. 1993;4:601–10.
Article
CAS
Google Scholar