Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

Table 1 Effects of enzyme combinations on the yield and viability of protoplasts isolated from leaves of cassava

Cellulase R-10 (W/V)	Macerozyme R-10 (W/V)	Protoplast yield (10⁷#/g FW)	Protoplast viability (%)
0.8	0.4	1.08 ± 0.14f	93.64 ± 1.00a
0.8	0.8	1.45 ± 0.09ef	93.05 ± 3.00a
0.8	1.2	1.89 ± 0.14e	91.32 ± 1.00a
1.6	0.4	3.08 ± 0.14d	93.43 ± 2.00a
1.6	0.8	4.42 ± 0.29b	92.60 ± 2.00a
1.6	1.2	3.25 ± 0.39 cd	90.14 ± 3.00a
2.4	0.4	1.80 ± 0.15e	88.38 ± 4.00ab
2.4	0.8	3.66 ± 0.18c	87.67 ± 1.00ab
2.4	1.2	5.06 ± 0.25a	83.82 ± 5.00b

Note: The averages of three technical replicates ± standard error of the mean (SEM) are shown. The different letters indicate significant differences (P ≤ 0.05) according to the Duncan test

ISSN: 1472-6750