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Table 1 Effects of enzyme combinations on the yield and viability of protoplasts isolated from leaves of cassava

From: Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

Cellulase R-10 (W/V) Macerozyme R-10 (W/V) Protoplast yield (107#/g FW) Protoplast viability (%)
0.8 0.4 1.08 ± 0.14f 93.64 ± 1.00a
0.8 0.8 1.45 ± 0.09ef 93.05 ± 3.00a
0.8 1.2 1.89 ± 0.14e 91.32 ± 1.00a
1.6 0.4 3.08 ± 0.14d 93.43 ± 2.00a
1.6 0.8 4.42 ± 0.29b 92.60 ± 2.00a
1.6 1.2 3.25 ± 0.39 cd 90.14 ± 3.00a
2.4 0.4 1.80 ± 0.15e 88.38 ± 4.00ab
2.4 0.8 3.66 ± 0.18c 87.67 ± 1.00ab
2.4 1.2 5.06 ± 0.25a 83.82 ± 5.00b
  1. Note: The averages of three technical replicates ± standard error of the mean (SEM) are shown. The different letters indicate significant differences (P ≤ 0.05) according to the Duncan test