Fig. 5From: Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)Subcellular localization of MeSTP7 in cassava mesophyll protoplasts. Transient expression of GFP, showing that the GFP is distributed throughout the nucleus and cytoplasm (a-d). The laser-scanning confocal microscopy images are the bright field image (a), fluorescence image (b), merged image (c) and autofluorescence image (d), respectively. The transient expression of GFP-fused MeSTP7 protein, showing that the MeSTP7-GFP fusion protein is likely localized to plasma membrane (e-h). The laser-scanning confocal microscopy images are the bright field image (e), fluorescence image (f), merged image (g) and chloroplast fluorescence (H), respectively. The bars = 5 μm.)Back to article page