Fig. 6From: Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)Subcellular localization analysis of MeSTP7 in Arabidopsis mesophyll protoplasts. Transient expression of GFP, showing that the GFP is distributed throughout the nucleus and cytoplasm (a-d). The laser-scanning confocal microscopy images are the bright field image (a), fluorescence image (b), merged image (c) and chloroplast fluorescence image (d), respectively. The transient expression of GFP-fused MeSTP7 protein, showing that the MeSTP7-GFP fusion protein is likely localized to cytoplasm membrane (e-h). The laser-scanning confocal microscopy images are the bright field image (e), fluorescence image (f), merged image (g) and chloroplast fluorescence image (h), respectively. The bars = 10 μmBack to article page