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  1. Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth in...

    Authors: Xiu-Mei Cai, Hai-Long Xie, Ming-Zhu Liu and Xi-Liang Zha

    Citation: BMC Biotechnology 2008 8:74

    Content type: Methodology article

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  2. The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by a [Fe-Fe] hydrogenase HydA1. For further biochemical and biophysical studies a suitable exp...

    Authors: Kateryna Sybirna, Tatiana Antoine, Pia Lindberg, Vincent Fourmond, Marc Rousset, Vincent Méjean and Hervé Bottin

    Citation: BMC Biotechnology 2008 8:73

    Content type: Research article

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  3. Articular cartilage is exposed to high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of cartilaginous matrix, in response to mechanical signals. How...

    Authors: Carole Bougault, Anne Paumier, Elisabeth Aubert-Foucher and Frédéric Mallein-Gerin

    Citation: BMC Biotechnology 2008 8:71

    Content type: Methodology article

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  4. The aim of this experimental study on New Zealand's white rabbits was to find differences in the results of treating the distal physeal femoral defect by the transplantation of autologous or allogeneic mesench...

    Authors: Ladislav Planka, Petr Gal, Helga Kecova, Jiri Klima, Jana Hlucilova, Eva Filova, Evzen Amler, Petr Krupa, Leos Kren, Robert Srnec, Lucie Urbanova, Jana Lorenzova and Alois Necas

    Citation: BMC Biotechnology 2008 8:70

    Content type: Research article

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  5. Determining the expression levels of microRNAs (miRNAs) is of great interest to researchers in many areas of biology, given the significant roles these molecules play in cellular regulation. Two common methods...

    Authors: Robert A Ach, Hui Wang and Bo Curry

    Citation: BMC Biotechnology 2008 8:69

    Content type: Research article

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  6. The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable...

    Authors: Alessandra Mallano, Silvia Zamboni, Giulia Carpinelli, Filippo Santoro, Michela Flego, Alessandro Ascione, Mara Gellini, Marina Tombesi, Franca Podo and Maurizio Cianfriglia

    Citation: BMC Biotechnology 2008 8:68

    Content type: Research article

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  7. Phosphoribosyl pyrophosphate (PRPP) is a central compound for cellular metabolism and may be considered as a link between carbon and nitrogen metabolism. PRPP is directly involved in the de novo and salvage biosy...

    Authors: Alberto Jiménez, María A Santos and José L Revuelta

    Citation: BMC Biotechnology 2008 8:67

    Content type: Research article

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  8. Venezuelan equine encephalitis virus (VEEV) belongs to the Alphavirus group. Several species of this family are also pathogenic to humans and are recognized as potential agents of biological warfare and terror...

    Authors: Martina Inga Kirsch, Birgit Hülseweh, Christoph Nacke, Torsten Rülker, Thomas Schirrmann, Hans-Jürgen Marschall, Michael Hust and Stefan Dübel

    Citation: BMC Biotechnology 2008 8:66

    Content type: Research article

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  9. Mammalian cells are becoming the prevailing expression system for the production of recombinant proteins because of their capacity for proper protein folding, assembly, and post-translational modifications. Th...

    Authors: Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh and Yves Durocher

    Citation: BMC Biotechnology 2008 8:65

    Content type: Research article

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  10. Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies...

    Authors: Helene Y Meller Harel, Veronique Fontaine, Hongying Chen, Ian M Jones and Paul A Millner

    Citation: BMC Biotechnology 2008 8:64

    Content type: Methodology article

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  11. The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under...

    Authors: Natalia I Minaeva, Evgeny R Gak, Danila V Zimenkov, Aleksandra Yu Skorokhodova, Irina V Biryukova and Sergey V Mashko

    Citation: BMC Biotechnology 2008 8:63

    Content type: Methodology article

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  12. Beta-thalassemia is a common autosomal recessive hereditary disease in the Meditertanean, Asia and African areas. Over 600 mutations have been described in the beta-globin (HBB), of which more than 200 are associ...

    Authors: Chia-Cheng Hung, Yi-Ning Su, Chia-Yun Lin, Yin-Fei Chang, Chien-Hui Chang, Wen-Fang Cheng, Chi-An Chen, Chien-Nan Lee and Win-Li Lin

    Citation: BMC Biotechnology 2008 8:62

    Content type: Methodology article

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  13. Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending supp...

    Authors: Brian Moldt, Nicklas H Staunstrup, Maria Jakobsen, Rafael J Yáñez-Muñoz and Jacob G Mikkelsen

    Citation: BMC Biotechnology 2008 8:60

    Content type: Research article

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  14. Bacteria use N-acyl homoserine lactone (AHL) molecules to regulate the expression of genes in a density-dependent manner. Several biosensors have been developed and engineered to detect the presence of all types ...

    Authors: Steve P Bernier, Anne L Beeston and Pamela A Sokol

    Citation: BMC Biotechnology 2008 8:59

    Content type: Methodology article

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  15. Cell-free protein synthesis is not only a rapid and high throughput technology to obtain proteins from their genes, but also provides an in vitro platform to study protein translation and folding. A detailed comp...

    Authors: Jason R Hillebrecht and Shaorong Chong

    Citation: BMC Biotechnology 2008 8:58

    Content type: Research article

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  16. Human papilloma virus (HPV) load and physical status are considered useful parameters for clinical evaluation of cervical squamous cell neoplasia. However, the errors implicit in HPV gene quantification by PCR...

    Authors: Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff and Nicholas Coleman

    Citation: BMC Biotechnology 2008 8:57

    Content type: Research article

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  17. Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult t...

    Authors: Monika Witusik, Sylwester Piaskowski, Krystyna Hulas-Bigoszewska, Magdalena Zakrzewska, Sylwia M Gresner, S Ausim Azizi, Barbara Krynska, Pawel P Liberski and Piotr Rieske

    Citation: BMC Biotechnology 2008 8:56

    Content type: Research article

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  18. Regulated protein-protein interactions (PPIs) are pivotal molecular switches that are important for the regulation of signaling processes within eukaryotic cells. Cellular signaling is altered in various disea...

    Authors: Michael C Wehr, Lisa Reinecke, Anna Botvinnik and Moritz J Rossner

    Citation: BMC Biotechnology 2008 8:55

    Content type: Methodology article

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  19. The meniscus is a complex tissue whose cell biology has only recently begun to be explored. Published models rely upon initial culture in the presence of added growth factors. The aim of this study was to test...

    Authors: Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley Jr and Yubo Sun

    Citation: BMC Biotechnology 2008 8:54

    Content type: Research article

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  20. Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the developm...

    Authors: Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson and Edward P Rybicki

    Citation: BMC Biotechnology 2008 8:53

    Content type: Research article

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  21. Establishing a suitable level of exogenous gene expression in mammalian cells in general, and embryonic stem (ES) cells in particular, is an important aspect of understanding pathways of cell differentiation, ...

    Authors: Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro and J Richard Chaillet

    Citation: BMC Biotechnology 2008 8:52

    Content type: Methodology article

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  22. One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for ...

    Authors: Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross and Fei P Gao

    Citation: BMC Biotechnology 2008 8:51

    Content type: Methodology article

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  23. Human butyrylcholinesterase (huBChE) has been shown to be an effective antidote against multiple LD50 of organophosphorus compounds. A prerequisite for such use of huBChE is a prolonged circulatory half-life. Thi...

    Authors: Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, Mélanie Côté, Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle Bégin, Nathalie Neveu, Eric Brochu…

    Citation: BMC Biotechnology 2008 8:50

    Content type: Research article

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  24. Using cell-type-specific promoters to restrict gene expression to particular cells is an attractive approach for gene therapy, but often hampered by insufficient transcriptional activity of these promoters. Pr...

    Authors: Beihui Liu, Julian F Paton and Sergey Kasparov

    Citation: BMC Biotechnology 2008 8:49

    Content type: Research article

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  25. The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. R...

    Authors: Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel and Albrecht Ott

    Citation: BMC Biotechnology 2008 8:48

    Content type: Research article

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  26. Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construct...

    Authors: Robert G Rutledge and Don Stewart

    Citation: BMC Biotechnology 2008 8:47

    Content type: Methodology article

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  27. To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary ph...

    Authors: Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry and Martin Clynes

    Citation: BMC Biotechnology 2008 8:42

    Content type: Research article

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  28. Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins in vivo can be...

    Authors: Andrea Predonzani, Francesca Arnoldi, Alejandro López-Requena and Oscar R Burrone

    Citation: BMC Biotechnology 2008 8:41

    Content type: Research article

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  29. Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simu...

    Authors: Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2008 8:40

    Content type: Methodology article

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  30. Although many nerve prostheses have been proposed in recent years, in the case of consistent loss of nervous tissue peripheral nerve injury is still a traumatic pathology that may impair patient's movements by...

    Authors: Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi and Fabrizio Gelain

    Citation: BMC Biotechnology 2008 8:39

    Content type: Research article

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  31. Cryopreservation of preantral follicles or ovarian tissues would enable the storage of large numbers of primordial follicles or preantral follicles and preserves the structural integrity of somatic and reprodu...

    Authors: Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu and Teng-Tsao Hsu

    Citation: BMC Biotechnology 2008 8:38

    Content type: Methodology article

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  32. Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently emerged as bioreactors for the production of human therapeutic glycoproteins (va...

    Authors: Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung and Jae-Heung Jeon

    Citation: BMC Biotechnology 2008 8:36

    Content type: Research article

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  33. Exon skipping oligonucleotides (ESOs) of 2'O-Methyl (2'OMe) and morpholino chemistry have been shown to restore dystrophin expression in muscle fibers from the mdx mouse, and are currently being tested in phase I...

    Authors: Jason H Williams, Rebecca C Schray, Shashank R Sirsi and Gordon J Lutz

    Citation: BMC Biotechnology 2008 8:35

    Content type: Research article

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  34. The Rho GTPases A, B and C proteins, members of the Rho family whose activity is regulated by GDP/GTP cycling, function in many cellular pathways controlling proliferation and have recently been implicated in ...

    Authors: Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre and Jean-Charles Faye

    Citation: BMC Biotechnology 2008 8:34

    Content type: Research article

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  35. Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastori...

    Authors: Maria Ruottinen, Monika Bollok, Martin Kögler, Antje Neubauer, Mirja Krause, Eija-Riitta Hämäläinen, Johanna Myllyharju, Antti Vasala and Peter Neubauer

    Citation: BMC Biotechnology 2008 8:33

    Content type: Methodology article

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  36. The last decade has brought the renaissance of protein studies and accelerated the development of high-throughput methods in all aspects of proteomics. Presently, most protein synthesis systems exploit the cap...

    Authors: Viola Bardóczy, Viktória Géczi, Tatsuya Sawasaki, Yaeta Endo and Tamás Mészáros

    Citation: BMC Biotechnology 2008 8:32

    Content type: Methodology article

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  37. Gene targeting in nonhuman primates has the potential to produce critical animal models for translational studies related to human diseases. Successful gene targeting in fibroblasts followed by somatic cell nu...

    Authors: Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette and Robert B Norgren Jr

    Citation: BMC Biotechnology 2008 8:31

    Content type: Methodology article

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  38. β-Lactams like penicillin and cephalosporin are among the oldest known antibiotics used against bacterial infections. Industrially, penicillin is produced by the filamentous fungus Penicillium chrysogenum. Our go...

    Authors: Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis and Jan AKW Kiel

    Citation: BMC Biotechnology 2008 8:29

    Content type: Research article

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  39. Soybeans play a prominent role in allergologic research due to the high incidence of allergic reactions. For detailed studies on specific proteins it is necessary to have access to a large amount of pure subst...

    Authors: Eva Sewekow, Lars Christian Kessler, Andreas Seidel-Morgenstern and Hermann-Josef Rothkötter

    Citation: BMC Biotechnology 2008 8:27

    Content type: Methodology article

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  40. The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying ...

    Authors: Meti Buh Gašparič, Katarina Cankar, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2008 8:26

    Content type: Methodology article

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  41. In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduce...

    Authors: Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma and Jan Szopa

    Citation: BMC Biotechnology 2008 8:25

    Content type: Research article

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