Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Articles

Page 26 of 30

  1. Electrotransfer of plasmid DNA into skeletal muscle is a promising strategy for the delivery of therapeutic molecules targeting various muscular diseases, cancer and lower-limb ischemia. Internal Ribosome Entr...

    Authors: Camille Allera-Moreau, Aurélie Delluc-Clavières, Caroline Castano, Loïc Van den Berghe, Muriel Golzio, Marc Moreau, Justin Teissié, Jean-François Arnal and Anne-Catherine Prats

    Citation: BMC Biotechnology 2007 7:74

    Content type: Methodology article

    Published on:

  2. The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to consti...

    Authors: Shan Goh, Andrea Camattari, Daniel Ng, Ruth Song, Kevin Madden, Janet Westpheling and Victor VT Wong

    Citation: BMC Biotechnology 2007 7:72

    Content type: Research article

    Published on:

  3. Genomics tools, particularly DNA microarrays, have found application in a number of areas including gene discovery and disease characterization. Despite the vast utility of these tools, little work has been do...

    Authors: Pratik Jaluria, Michael Betenbaugh, Konstantinos Konstantopoulos and Joseph Shiloach

    Citation: BMC Biotechnology 2007 7:71

    Content type: Research article

    Published on:

  4. There is much evidence that tumor cells elicit a humoral immune response in patients. In most cases, the presence of antibodies in peripheral blood is detected only in small proportion of patients with tumors ...

    Authors: Emiliano Pavoni, Giorgia Monteriù, Daniela Santapaola, Fiorella Petronzelli, Anna Maria Anastasi, Angela Pelliccia, Valeria D'Alessio, Rita De Santis and Olga Minenkova

    Citation: BMC Biotechnology 2007 7:70

    Content type: Research article

    Published on:

  5. In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g. h...

    Authors: Magnus Stougaard, Jakob S Lohmann, Magdalena Zajac, Stephen Hamilton-Dutoit and Jørn Koch

    Citation: BMC Biotechnology 2007 7:69

    Content type: Methodology article

    Published on:

  6. The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capabi...

    Authors: Gaetano Donofrio, Chiara Sartori, Lara Ravanetti, Sandro Cavirani, Laurent Gillet, Alain Vanderplasschen, Simone Taddei and Cesidio Filippo Flammini

    Citation: BMC Biotechnology 2007 7:68

    Content type: Research article

    Published on:

  7. Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled...

    Authors: Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao and Joseph C Wu

    Citation: BMC Biotechnology 2007 7:67

    Content type: Research article

    Published on:

  8. High content screening (HCS)-based image analysis is becoming an important and widely used research tool. Capitalizing this technology, ample cellular information can be extracted from the high content cellula...

    Authors: Fuhai Li, Xiaobo Zhou, Jinmin Zhu, Jinwen Ma, Xudong Huang and Stephen TC Wong

    Citation: BMC Biotechnology 2007 7:66

    Content type: Research article

    Published on:

  9. Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic pha...

    Authors: Lauren RH Krumpe, Kathryn M Schumacher, James B McMahon, Lee Makowski and Toshiyuki Mori

    Citation: BMC Biotechnology 2007 7:65

    Content type: Methodology article

    Published on:

  10. The growing field of proteomics and systems biology is resulting in an ever increasing demand for purified recombinant proteins for structural and functional studies. Here, we show a systematic approach to suc...

    Authors: Claudia Langlais, Birgit Guilleaume, Nadja Wermke, Tina Scheuermann, Lars Ebert, Joshua LaBaer and Bernhard Korn

    Citation: BMC Biotechnology 2007 7:64

    Content type: Research article

    Published on:

  11. Plant viruses can be employed as versatile vectors for the production of vaccines by expressing immunogenic epitopes on the surface of chimeric viral particles. Although several viruses, including tobacco mosa...

    Authors: Chung-Da Yang, Jia-Teh Liao, Chen-Yen Lai, Ming-Hwa Jong, Chi-Ming Liang, Yeou-Liang Lin, Na-Sheng Lin, Yau-Heiu Hsu and Shu-Mei Liang

    Citation: BMC Biotechnology 2007 7:62

    Content type: Research article

    Published on:

  12. Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be ind...

    Authors: Daniel C Gray, Klaus P Hoeflich, Li Peng, Zhenyu Gu, Alvin Gogineni, Lesley J Murray, Mike Eby, Noelyn Kljavin, Somasekar Seshagiri, Mary J Cole and David P Davis

    Citation: BMC Biotechnology 2007 7:61

    Content type: Methodology article

    Published on:

  13. Microfluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practic...

    Authors: Bong Geun Chung, Jeong Won Park, Jia Sheng Hu, Carlos Huang, Edwin S Monuki and Noo Li Jeon

    Citation: BMC Biotechnology 2007 7:60

    Content type: Methodology article

    Published on:

  14. A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologo...

    Authors: Mandy M Cox, Sherryll L Layton, Tieshan Jiang, Kim Cole, Billy M Hargis, Luc R Berghman, Walter G Bottje and Young Min Kwon

    Citation: BMC Biotechnology 2007 7:59

    Content type: Methodology article

    Published on:

  15. Bacteriophages infecting lactic acid bacteria (LAB) are widely acknowledged as the main cause of milk fermentation failures. In this study, we describe the surface-expression as well as the secretion of two fu...

    Authors: Anna Hultberg, Denise M Tremblay, Hans de Haard, Theo Verrips, Sylvain Moineau, Lennart Hammarström and Harold Marcotte

    Citation: BMC Biotechnology 2007 7:58

    Content type: Research article

    Published on:

  16. The interpretability of microarray data can be affected by sample quality. To systematically explore how RNA quality affects microarray assay performance, a set of rat liver RNA samples with a progressive chan...

    Authors: Karol L Thompson, P Scott Pine, Barry A Rosenzweig, Yaron Turpaz and Jacques Retief

    Citation: BMC Biotechnology 2007 7:57

    Content type: Research article

    Published on:

  17. We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for a...

    Authors: Thomas O Kohl, Inga I Hitzeroth, Neil D Christensen and Edward P Rybicki

    Citation: BMC Biotechnology 2007 7:56

    Content type: Research article

    Published on:

  18. Bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. Among them, strains of the genus Pediococcus are particularly interesting for their ability to produce pediocin, a broa...

    Authors: Sophie Mathys, Ueli von Ah, Christophe Lacroix, Ernö Staub, Raffaella Mini, Tania Cereghetti and Leo Meile

    Citation: BMC Biotechnology 2007 7:55

    Content type: Research article

    Published on:

  19. Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the kinetically controlled synthesis of semi-synthetic ...

    Authors: Davide A Cecchini, Immacolata Serra, Daniela Ubiali, Marco Terreni and Alessandra M Albertini

    Citation: BMC Biotechnology 2007 7:54

    Content type: Research article

    Published on:

  20. Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and ...

    Authors: Mahiran Basri, Raja Noor Zaliha Raja Abd Rahman, Afshin Ebrahimpour, Abu Bakar Salleh, Erin Ryantin Gunawan and Mohd Basyaruddin Abd Rahman

    Citation: BMC Biotechnology 2007 7:53

    Content type: Research article

    Published on:

  21. The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising im...

    Authors: Li-Te Chin, Chishih Chu, Han-Min Chen, Shu-Ching Hsu, Bor-Chun Weng and Chi-Hong Chu

    Citation: BMC Biotechnology 2007 7:51

    Content type: Research article

    Published on:

  22. The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long olig...

    Authors: Jakob S Lohmann, Magnus Stougaard and Jørn Koch

    Citation: BMC Biotechnology 2007 7:49

    Content type: Methodology article

    Published on:

  23. As a new class of therapeutic and diagnostic reagents, more than fifteen years ago RNA and DNA aptamers were identified as binding molecules to numerous small compounds, proteins and rarely even to complete pa...

    Authors: Andreas Nitsche, Andreas Kurth, Anna Dunkhorst, Oliver Pänke, Hendrik Sielaff, Wolfgang Junge, Doreen Muth, Frieder Scheller, Walter Stöcklein, Claudia Dahmen, Georg Pauli and Andreas Kage

    Citation: BMC Biotechnology 2007 7:48

    Content type: Methodology article

    Published on:

  24. Promoters with tissue-specificity are desirable to drive expression of transgenes in crops to avoid accumulation of foreign proteins in edible tissues/organs. Several photosynthetic promoters have been shown t...

    Authors: Carole L Bassett, Ann M Callahan, Timothy S Artlip, Ralph Scorza and Chinnathambi Srinivasan

    Citation: BMC Biotechnology 2007 7:47

    Content type: Methodology article

    Published on:

  25. Phage display antibody libraries have been made from the lymphocytes of patients suffering from autoimmune diseases in which the antibodies are known to play a role in the pathogenesis or are important for the...

    Authors: Roberto Di Niro, Federica Ziller, Fiorella Florian, Sergio Crovella, Marco Stebel, Marco Bestagno, Oscar Burrone, Andrew RM Bradbury, Paola Secco, Roberto Marzari and Daniele Sblattero

    Citation: BMC Biotechnology 2007 7:46

    Content type: Research article

    Published on:

  26. Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/...

    Authors: Yunjia Chen, Shihong Qiu, Chi-Hao Luan and Ming Luo

    Citation: BMC Biotechnology 2007 7:45

    Content type: Methodology article

    Published on:

  27. Since prion gene-knockout mice do not contract prion diseases and animals in which production of prion protein (PrP) is reduced by half are resistant to the disease, we hypothesized that bovine animals with re...

    Authors: Shizuyo Sutou, Miho Kunishi, Toshiyuki Kudo, Pimprapar Wongsrikeao, Makoto Miyagishi and Takeshige Otoi

    Citation: BMC Biotechnology 2007 7:44

    Content type: Research article

    Published on:

  28. Swine is an important agricultural commodity and biomedical model. Manipulation of the pig genome provides opportunity to improve production efficiency, enhance disease resistance, and add value to swine produ...

    Authors: Karl J Clark, Daniel F Carlson, Linda K Foster, Byung-Whi Kong, Douglas N Foster and Scott C Fahrenkrug

    Citation: BMC Biotechnology 2007 7:42

    Content type: Research article

    Published on:

  29. RNA interference (RNAi)-mediated by the expression of short hairpin RNAs (shRNAs) has emerged as a powerful experimental tool for reverse genetic studies in mammalian cells. A number of recent reports have des...

    Authors: Krzysztof Pluta, William Diehl, Xian-Yang Zhang, Robert Kutner, Agnieszka Bialkowska and Jakob Reiser

    Citation: BMC Biotechnology 2007 7:41

    Content type: Research article

    Published on:

  30. The zebrafish Danio rerio is an important model system for drug discovery and to study cardiovascular development. Using a laser-scanning confocal microscope, we have developed a non-invasive method of measuring ...

    Authors: Michael H Malone, Noah Sciaky, Lisa Stalheim, Klaus M Hahn, Elwood Linney and Gary L Johnson

    Citation: BMC Biotechnology 2007 7:40

    Content type: Methodology article

    Published on:

  31. The simultaneous production of various recombinant proteins in every cell of a culture is often needed for the production of virus-like particles (VLP) or vectors for gene therapy. A common approach for such a...

    Authors: Jimmy A Mena, Octavio T Ramírez and Laura A Palomares

    Citation: BMC Biotechnology 2007 7:39

    Content type: Research article

    Published on:

  32. A hallmark of prion disease is the transformation of normal cellular prion protein (PrPc) into an infectious disease-associated isoform, (PrPsc). Anti-prion protein monoclonal antibodies are invaluable for str...

    Authors: Michela Flego, Alessandro Ascione, Silvia Zamboni, Maria L Dupuis, Valentina Imperiale and Maurizio Cianfriglia

    Citation: BMC Biotechnology 2007 7:38

    Content type: Research article

    Published on:

  33. Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in livi...

    Authors: Ekaterina A Souslova, Vsevolod V Belousov, John G Lock, Staffan Strömblad, Sergey Kasparov, Alexey P Bolshakov, Vsevolod G Pinelis, Yulii A Labas, Sergey Lukyanov, Lorenz M Mayr and Dmitriy M Chudakov

    Citation: BMC Biotechnology 2007 7:37

    Content type: Research article

    Published on:

  34. Archival formalin-fixed paraffin-embedded (FFPE) tissues have limited utility in applications involving analysis of gene expression due to mRNA degradation and modification during fixation and processing. This...

    Authors: Jinghuan Li, Paul Smyth, Richard Flavin, Susanne Cahill, Karen Denning, Sinead Aherne, Simone M Guenther, John J O'Leary and Orla Sheils

    Citation: BMC Biotechnology 2007 7:36

    Content type: Research article

    Published on:

  35. Interleukin-12 (IL-12) is well characterized to induce cellular antitumoral immunity by activation of NK-cells and T-lymphocytes. However, systemic administration of recombinant human IL-12 resulted in severe ...

    Authors: Holger Wulff, Thorsten Krieger, Karen Krüger, Ingrid Stahmer, Friedrich Thaiss, Hansjörg Schäfer and Andreas Block

    Citation: BMC Biotechnology 2007 7:35

    Content type: Research article

    Published on:

  36. Nowadays, the focus in metabolic engineering research is shifting from massive overexpression and inactivation of genes towards the model-based fine tuning of gene expression. In this context, the construction...

    Authors: Marjan De Mey, Jo Maertens, Gaspard J Lequeux, Wim K Soetaert and Erick J Vandamme

    Citation: BMC Biotechnology 2007 7:34

    Content type: Research article

    Published on:

  37. Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming pr...

    Authors: Kostyantyn V Dmytruk, Oleh V Smutok, Olena B Ryabova, Galyna Z Gayda, Volodymyr A Sibirny, Wolfgang Schuhmann, Mykhailo V Gonchar and Andriy A Sibirny

    Citation: BMC Biotechnology 2007 7:33

    Content type: Research article

    Published on:

  38. The overproduction of recombinant proteins in host cells often leads to their misfolding and aggregation. Previous attempts to increase the solubility of recombinant proteins by co-overproduction of individual...

    Authors: Ario de Marco, Elke Deuerling, Axel Mogk, Toshifumi Tomoyasu and Bernd Bukau

    Citation: BMC Biotechnology 2007 7:32

    Content type: Research article

    Published on:

  39. Many structural biology- and high-throughput laboratories experience the acquisition of multiple cDNAs from different sources as a rather time- and resource-consuming procedure. The techniques presented here s...

    Authors: Daniel Frey, Christian Kambach, Michel O Steinmetz and Rolf Jaussi

    Citation: BMC Biotechnology 2007 7:31

    Content type: Methodology article

    Published on:

  40. Immune-mediated rejection of labeled cells is a general problem in transplantation studies using cells labeled with any immunogenic marker, and also in gene therapy protocols. The aim of this study was to esta...

    Authors: Kathrin I Odörfer, Nina J Unger, Karin Weber, Eric P Sandgren and Reinhold G Erben

    Citation: BMC Biotechnology 2007 7:30

    Content type: Methodology article

    Published on:

  41. The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects....

    Authors: Maxim Pimkin, Elena Caretti, Adrian Canutescu, Jeffrey B Yeung, Heather Cohn, Yibai Chen, Catherine Oleykowski, Alfonso Bellacosa and Anthony T Yeung

    Citation: BMC Biotechnology 2007 7:29

    Content type: Research article

    Published on:

  42. Biologically relevant molecular markers can be used in evaluation of the physiological state of an organism in biotechnical processes. We monitored at high frequency the expression of 34 marker genes in batch,...

    Authors: Jari J Rautio, Michael Bailey, Teemu Kivioja, Hans Söderlund, Merja Penttilä and Markku Saloheimo

    Citation: BMC Biotechnology 2007 7:28

    Content type: Research article

    Published on:

  43. Codon usage differences are known to regulate the levels of gene expression in a species-specific manner, with the primary factors often cited to be mRNA processing and accumulation. We have challenged this co...

    Authors: Brian C Geyer, Samuel P Fletcher, Tagan A Griffin, Michael J Lopker, Hermona Soreq and Tsafrir S Mor

    Citation: BMC Biotechnology 2007 7:27

    Content type: Research article

    Published on:

  44. There are no published studies on stem cells from equine cord blood although commercial storage of equine cord blood for future autologous stem cell transplantations is available. Mesenchymal stem cells (MSC) ...

    Authors: Thomas G Koch, Tammy Heerkens, Preben D Thomsen and Dean H Betts

    Citation: BMC Biotechnology 2007 7:26

    Content type: Research article

    Published on:

  45. Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great...

    Authors: Toshinari Maeda, Gönül Vardar, William T Self and Thomas K Wood

    Citation: BMC Biotechnology 2007 7:25

    Content type: Research article

    Published on: