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  1. The simultaneous production of various recombinant proteins in every cell of a culture is often needed for the production of virus-like particles (VLP) or vectors for gene therapy. A common approach for such a...

    Authors: Jimmy A Mena, Octavio T Ramírez and Laura A Palomares

    Citation: BMC Biotechnology 2007 7:39

    Content type: Research article

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  2. A hallmark of prion disease is the transformation of normal cellular prion protein (PrPc) into an infectious disease-associated isoform, (PrPsc). Anti-prion protein monoclonal antibodies are invaluable for str...

    Authors: Michela Flego, Alessandro Ascione, Silvia Zamboni, Maria L Dupuis, Valentina Imperiale and Maurizio Cianfriglia

    Citation: BMC Biotechnology 2007 7:38

    Content type: Research article

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  3. Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in livi...

    Authors: Ekaterina A Souslova, Vsevolod V Belousov, John G Lock, Staffan Strömblad, Sergey Kasparov, Alexey P Bolshakov, Vsevolod G Pinelis, Yulii A Labas, Sergey Lukyanov, Lorenz M Mayr and Dmitriy M Chudakov

    Citation: BMC Biotechnology 2007 7:37

    Content type: Research article

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  4. Archival formalin-fixed paraffin-embedded (FFPE) tissues have limited utility in applications involving analysis of gene expression due to mRNA degradation and modification during fixation and processing. This...

    Authors: Jinghuan Li, Paul Smyth, Richard Flavin, Susanne Cahill, Karen Denning, Sinead Aherne, Simone M Guenther, John J O'Leary and Orla Sheils

    Citation: BMC Biotechnology 2007 7:36

    Content type: Research article

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  5. Interleukin-12 (IL-12) is well characterized to induce cellular antitumoral immunity by activation of NK-cells and T-lymphocytes. However, systemic administration of recombinant human IL-12 resulted in severe ...

    Authors: Holger Wulff, Thorsten Krieger, Karen Krüger, Ingrid Stahmer, Friedrich Thaiss, Hansjörg Schäfer and Andreas Block

    Citation: BMC Biotechnology 2007 7:35

    Content type: Research article

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  6. Nowadays, the focus in metabolic engineering research is shifting from massive overexpression and inactivation of genes towards the model-based fine tuning of gene expression. In this context, the construction...

    Authors: Marjan De Mey, Jo Maertens, Gaspard J Lequeux, Wim K Soetaert and Erick J Vandamme

    Citation: BMC Biotechnology 2007 7:34

    Content type: Research article

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  7. Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming pr...

    Authors: Kostyantyn V Dmytruk, Oleh V Smutok, Olena B Ryabova, Galyna Z Gayda, Volodymyr A Sibirny, Wolfgang Schuhmann, Mykhailo V Gonchar and Andriy A Sibirny

    Citation: BMC Biotechnology 2007 7:33

    Content type: Research article

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  8. The overproduction of recombinant proteins in host cells often leads to their misfolding and aggregation. Previous attempts to increase the solubility of recombinant proteins by co-overproduction of individual...

    Authors: Ario de Marco, Elke Deuerling, Axel Mogk, Toshifumi Tomoyasu and Bernd Bukau

    Citation: BMC Biotechnology 2007 7:32

    Content type: Research article

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  9. Many structural biology- and high-throughput laboratories experience the acquisition of multiple cDNAs from different sources as a rather time- and resource-consuming procedure. The techniques presented here s...

    Authors: Daniel Frey, Christian Kambach, Michel O Steinmetz and Rolf Jaussi

    Citation: BMC Biotechnology 2007 7:31

    Content type: Methodology article

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  10. Immune-mediated rejection of labeled cells is a general problem in transplantation studies using cells labeled with any immunogenic marker, and also in gene therapy protocols. The aim of this study was to esta...

    Authors: Kathrin I Odörfer, Nina J Unger, Karin Weber, Eric P Sandgren and Reinhold G Erben

    Citation: BMC Biotechnology 2007 7:30

    Content type: Methodology article

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  11. The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects....

    Authors: Maxim Pimkin, Elena Caretti, Adrian Canutescu, Jeffrey B Yeung, Heather Cohn, Yibai Chen, Catherine Oleykowski, Alfonso Bellacosa and Anthony T Yeung

    Citation: BMC Biotechnology 2007 7:29

    Content type: Research article

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  12. Biologically relevant molecular markers can be used in evaluation of the physiological state of an organism in biotechnical processes. We monitored at high frequency the expression of 34 marker genes in batch,...

    Authors: Jari J Rautio, Michael Bailey, Teemu Kivioja, Hans Söderlund, Merja Penttilä and Markku Saloheimo

    Citation: BMC Biotechnology 2007 7:28

    Content type: Research article

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  13. Codon usage differences are known to regulate the levels of gene expression in a species-specific manner, with the primary factors often cited to be mRNA processing and accumulation. We have challenged this co...

    Authors: Brian C Geyer, Samuel P Fletcher, Tagan A Griffin, Michael J Lopker, Hermona Soreq and Tsafrir S Mor

    Citation: BMC Biotechnology 2007 7:27

    Content type: Research article

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  14. There are no published studies on stem cells from equine cord blood although commercial storage of equine cord blood for future autologous stem cell transplantations is available. Mesenchymal stem cells (MSC) ...

    Authors: Thomas G Koch, Tammy Heerkens, Preben D Thomsen and Dean H Betts

    Citation: BMC Biotechnology 2007 7:26

    Content type: Research article

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  15. Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great...

    Authors: Toshinari Maeda, Gönül Vardar, William T Self and Thomas K Wood

    Citation: BMC Biotechnology 2007 7:25

    Content type: Research article

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  16. Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome (BAC) technology. Such modified genomes have potential uses in understanding viral pathogenesi...

    Authors: Falko Schmeisser and Jerry P Weir

    Citation: BMC Biotechnology 2007 7:22

    Content type: Methodology article

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  17. The ability to accurately measure patterns of gene expression is essential in studying gene function. The reverse transcription polymerase chain reaction (RT-PCR) has become the method of choice for the detect...

    Authors: Fadia Haddad, Anqi X Qin, Julie M Giger, Hongyan Guo and Kenneth M Baldwin

    Citation: BMC Biotechnology 2007 7:21

    Content type: Methodology article

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  18. Angiogenesis assays are important tools for the identification of regulatory molecules and the potential development of therapeutic strategies to modulate neovascularization. Although numerous in vitro angioge...

    Authors: Bastien Hermant, Agnès Desroches-Castan, Marie-Laure Dubessay, Marie-Hélène Prandini, Philippe Huber and Daniel Vittet

    Citation: BMC Biotechnology 2007 7:20

    Content type: Methodology article

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  19. In protein drug development, in vitro molecular optimization or protein maturation can be used to modify protein properties. One basic approach to protein maturation is the introduction of random DNA mutations in...

    Authors: George Kopsidas, Rachael K Carman, Emma L Stutt, Anna Raicevic, Anthony S Roberts, Mary-Anne V Siomos, Nada Dobric, Luisa Pontes-Braz and Greg Coia

    Citation: BMC Biotechnology 2007 7:18

    Content type: Methodology article

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  20. Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers. However, eggs have found so far only limited use in the production of recombinant prote...

    Authors: Teresa Corral, Lorena S Ver, Geneviève Mottet, Olga Cano, Blanca García-Barreno, Lesley J Calder, John J Skehel, Laurent Roux and José A Melero

    Citation: BMC Biotechnology 2007 7:17

    Content type: Methodology article

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  21. Altering a protein's function by changing its sequence allows natural proteins to be converted into useful molecular tools. Current protein engineering methods are limited by a lack of high throughput physical...

    Authors: Jun Liao, Manfred K Warmuth, Sridhar Govindarajan, Jon E Ness, Rebecca P Wang, Claes Gustafsson and Jeremy Minshull

    Citation: BMC Biotechnology 2007 7:16

    Content type: Research article

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  22. A goal for the food industry has always been to improve strains of Lactococcus lactis and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while el...

    Authors: Maria Papagianni, Nicholaos Avramidis and George Filioussis

    Citation: BMC Biotechnology 2007 7:15

    Content type: Methodology article

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  23. The connection of the variable part of the heavy chain (VH) and and the variable part of the light chain (VL) by a peptide linker to form a consecutive polypeptide chain (single chain antibody, scFv) was a bre...

    Authors: Michael Hust, Thomas Jostock, Christian Menzel, Bernd Voedisch, Anja Mohr, Mariam Brenneis, Martina I Kirsch, Doris Meier and Stefan Dübel

    Citation: BMC Biotechnology 2007 7:14

    Content type: Research article

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  24. Bacteriophage infections of bacterial cultures cause serious problems in genetic engineering and biotechnology. They are dangerous not only because of direct effects on the currently infected cultures, i.e. th...

    Authors: Marcin Łoś, Piotr Golec, Joanna M Łoś, Anna Węglewska-Jurkiewicz, Agata Czyż, Alicja Węgrzyn, Grzegorz Węgrzyn and Peter Neubauer

    Citation: BMC Biotechnology 2007 7:13

    Content type: Correspondence

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  25. Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increa...

    Authors: Carla Marusic, James Nuttall, Giampaolo Buriani, Chiara Lico, Raffaele Lombardi, Selene Baschieri, Eugenio Benvenuto and Lorenzo Frigerio

    Citation: BMC Biotechnology 2007 7:12

    Content type: Research article

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  26. Strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which complicates the breeding of new cultivars. For certain traits, genetic engineering offers a potential alte...

    Authors: Kati J Hanhineva and Sirpa O Kärenlampi

    Citation: BMC Biotechnology 2007 7:11

    Content type: Methodology article

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  27. Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses...

    Authors: Saima Khanam, Pilankatta Rajendra, Navin Khanna and Sathyamangalam Swaminathan

    Citation: BMC Biotechnology 2007 7:10

    Content type: Research article

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  28. Despite several effective treatment options available for prostate cancer, it remains the second leading cause of cancer death in American men. Thus, there is a great need for new treatments to improve outcome...

    Authors: Jan Woraratanadharm, Semyon Rubinchik, Hong Yu and John Y Dong

    Citation: BMC Biotechnology 2007 7:9

    Content type: Research article

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  29. Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies. Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy...

    Authors: William S Branham, Cathy D Melvin, Tao Han, Varsha G Desai, Carrie L Moland, Adam T Scully and James C Fuscoe

    Citation: BMC Biotechnology 2007 7:8

    Content type: Methodology article

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  30. Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to ref...

    Authors: Aurelien Olichon, Daniel Schweizer, Serge Muyldermans and Ario de Marco

    Citation: BMC Biotechnology 2007 7:7

    Content type: Methodology article

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  31. The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems are based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding p...

    Authors: Xue Zhou, Jori Symons, Rieuwert Hoppes, Christel Krueger, Christian Berens, Wolfgang Hillen, Ben Berkhout and Atze T Das

    Citation: BMC Biotechnology 2007 7:6

    Content type: Research article

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  32. We constructed and characterized several new piggyBac vectors to provide transposition of constitutively- or inducibly-expressible heterologous gene pairs. The dual constitutive control element consists of back-t...

    Authors: Xianzong Shi, Robert L Harrison, Jason R Hollister, Ahmed Mohammed, Malcolm J Fraser Jr and Donald L Jarvis

    Citation: BMC Biotechnology 2007 7:5

    Content type: Research article

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  33. Fluorescence activated cell sorting (FACS) is a powerful technique for the qualitative and quantitative detection of biomolecules used widely in both basic research and clinical diagnostic applications. Beads ...

    Authors: B Thomas Bäckström, Jane A Brockelbank and Bernd HA Rehm

    Citation: BMC Biotechnology 2007 7:3

    Content type: Research article

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  34. Advances in fluorescence microscopy and mouse transgenesis have made it possible to image molecular events in living animals. However, the generation of transgenic mice is a lengthy process and intravital imag...

    Authors: Adriaan D Bins, Jacco van Rheenen, Kees Jalink, Jonathan R Halstead, Nullin Divecha, David M Spencer, John BAG Haanen and Ton NM Schumacher

    Citation: BMC Biotechnology 2007 7:2

    Content type: Methodology article

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  35. The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membr...

    Authors: Johanna Rinne, Brian Albarran, Juulia Jylhävä, Teemu O Ihalainen, Pasi Kankaanpää, Vesa P Hytönen, Patrick S Stayton, Markku S Kulomaa and Maija Vihinen-Ranta

    Citation: BMC Biotechnology 2007 7:1

    Content type: Research article

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  36. The availability of cell lines releasing fluorescent viral particles can significantly support a variety of investigations, including the study of virus-cell interaction and the screening of antiviral compound...

    Authors: Claudia Muratori, Paola D'Aloja, Fabiana Superti, Antonella Tinari, Nathalie Sol-Foulon, Sandra Sparacio, Valerie Bosch, Olivier Schwartz and Maurizio Federico

    Citation: BMC Biotechnology 2006 6:52

    Content type: Research article

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  37. Enzymes have been extensively used in organic solvents to catalyze a variety of transformations of biological and industrial significance. It has been generally accepted that in dry aprotic organic solvents, e...

    Authors: Betzaida Castillo, Vibha Bansal, Ashok Ganesan, Peter Halling, Francesco Secundo, Amaris Ferrer, Kai Griebenow and Gabriel Barletta

    Citation: BMC Biotechnology 2006 6:51

    Content type: Research article

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  38. RNA interference (RNAi) was originally identified as a biological process in which short double-stranded RNA (dsRNA) suppress the expression of genes complimentary to the dsRNA. This cellular intrinsic gene si...

    Authors: Shunqing Wang, Zhenqi Shi, Wei Liu, Joel Jules and Xu Feng

    Citation: BMC Biotechnology 2006 6:50

    Content type: Methodology article

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  39. A variety of approaches to understanding protein structure and function require production of recombinant protein. Mammalian based expression systems have advantages over bacterial systems for certain classes ...

    Authors: Susan DJ Chapple, Anna M Crofts, S Paul Shadbolt, John McCafferty and Michael R Dyson

    Citation: BMC Biotechnology 2006 6:49

    Content type: Research article

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  40. The signal transducer and activator of transcription (STAT) family of transcription factors mediates a variety of cytokine dependent gene regulations. STAT1 has been mainly characterized by its role in interfe...

    Authors: Nicole R Leitner, Birgit Strobl, Marion Bokor, Ronald Painz, Thomas Kolbe, Thomas Rülicke, Mathias Müller and Marina Karaghiosoff

    Citation: BMC Biotechnology 2006 6:48

    Content type: Research article

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  41. Isolation of recombinant antibody fragments from antibody libraries is well established using technologies such as phage display. Phage display vectors are ideal for efficient display of antibody fragments on ...

    Authors: Cecile D Martin, Gertrudis Rojas, Joanne N Mitchell, Karen J Vincent, Jiahua Wu, John McCafferty and Darren J Schofield

    Citation: BMC Biotechnology 2006 6:46

    Content type: Research article

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  42. Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant t...

    Authors: Federica Bestoso, Laura Ottaggio, Andrea Armirotti, Alessandro Balbi, Gianluca Damonte, Paolo Degan, Mauro Mazzei, Francesca Cavalli, Bernardetta Ledda and Mariangela Miele

    Citation: BMC Biotechnology 2006 6:45

    Content type: Research article

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  43. In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy fo...

    Authors: J Aquiles Sanchez, Jessica D Abramowitz, Jesse J Salk, Arthur H Reis Jr, John E Rice, Kenneth E Pierce and Lawrence J Wangh

    Citation: BMC Biotechnology 2006 6:44

    Content type: Methodology article

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  44. A number of expression systems have been developed where transgene expression can be regulated. They all have specific characteristics making them more suitable for certain applications than for others. Since ...

    Authors: Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie

    Citation: BMC Biotechnology 2006 6:43

    Content type: Methodology article

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  45. Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have no...

    Authors: Roderick F Felsheim, Michael J Herron, Curtis M Nelson, Nicole Y Burkhardt, Anthony F Barbet, Timothy J Kurtti and Ulrike G Munderloh

    Citation: BMC Biotechnology 2006 6:42

    Content type: Methodology article

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