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  1. Femtosecond (fs) laser pulses have recently received wide interest as an alternative tool for manipulating living biological systems. In various model organisms the excision of cellular components and the intr...

    Authors: Vikram Kohli and Abdulhakem Y Elezzabi

    Citation: BMC Biotechnology 2008 8:7

    Content type: Research article

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  2. For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneou...

    Authors: Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma and Dongzhi Wei

    Citation: BMC Biotechnology 2008 8:4

    Content type: Research article

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  3. The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such,...

    Authors: Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli and Leslie Lobel

    Citation: BMC Biotechnology 2008 8:3

    Content type: Research article

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  4. A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules.

    Authors: Angelika Wegerer, Tianqi Sun and Josef Altenbuchner

    Citation: BMC Biotechnology 2008 8:2

    Content type: Research article

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  5. Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis.

    Authors: Ricardo Pinto, António L Amaral, Eugénio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel and Miguel Gama

    Citation: BMC Biotechnology 2008 8:1

    Content type: Research article

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  6. HERG potassium channel blockade is the major cause for drug-induced long QT syndrome, which sometimes cause cardiac disrhythmias and sudden death. There is a strong interest in the pharmaceutical industry to d...

    Authors: Mao Xiang Chen, Shaun L Sandow, Virginie Doceul, Yu Hua Chen, Heather Harper, Bruce Hamilton, Helen J Meadows, Derek J Trezise and Jeff J Clare

    Citation: BMC Biotechnology 2007 7:93

    Content type: Research article

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  7. GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybri...

    Authors: Il-Han Kim, Jens Nagel, Simone Otten, Boris Knerr, Roland Eils, Karl Rohr and Steffen Dietzel

    Citation: BMC Biotechnology 2007 7:92

    Content type: Research article

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  8. Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed...

    Authors: Torstein Tengs, Anja B Kristoffersen, Knut G Berdal, Tage Thorstensen, Melinka A Butenko, Håvard Nesvold and Arne Holst-Jensen

    Citation: BMC Biotechnology 2007 7:91

    Content type: Methodology article

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  9. Bone marrow-derived stromal cells (MSC) are attractive targets for ex vivo cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (h)...

    Authors: Mark W Ronsyn, Jasmijn Daans, Gie Spaepen, Shyama Chatterjee, Katrien Vermeulen, Patrick D'Haese, Viggo FI Van Tendeloo, Eric Van Marck, Dirk Ysebaert, Zwi N Berneman, Philippe G Jorens and Peter Ponsaerts

    Citation: BMC Biotechnology 2007 7:90

    Content type: Research article

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  10. The acute sensitivity of some hybridoma cell lines to culture-related stresses severely limits their productivity. Recent developments in the characterization of the stress signals modulating the cellular phen...

    Authors: Matthew Mallory, Kevin Chartrand and Eric R Gauthier

    Citation: BMC Biotechnology 2007 7:89

    Content type: Research article

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  11. Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D in vivo tissue growth. Such realistic environmen...

    Authors: Thomas Liebmann, Susanna Rydholm, Victor Akpe and Hjalmar Brismar

    Citation: BMC Biotechnology 2007 7:88

    Content type: Research article

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  12. The formation of two distinctive cell lineages in preimplantation mouse embryos is characterized by differential gene expression. The cells of the inner cell mass are pluripotent and express high levels of Oct4 m...

    Authors: Cristina Hartshorn, Judith J Eckert, Odelya Hartung and Lawrence J Wangh

    Citation: BMC Biotechnology 2007 7:87

    Content type: Methodology article

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  13. Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation t...

    Authors: Barry J Ryan and Ciarán Ó'Fágáin

    Citation: BMC Biotechnology 2007 7:86

    Content type: Research article

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  14. Retroviral vectors are valuable tools for gene transfer. Particularly convenient are IRES-containing retroviral vectors expressing both the protein of interest and a marker protein from a single bicistronic mR...

    Authors: Olivier Albagli-Curiel, Yann Lécluse, Philippe Pognonec, Kim E Boulukos and Patrick Martin

    Citation: BMC Biotechnology 2007 7:85

    Content type: Methodology article

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  15. Antibody-dependent cellular cytotoxicity (ADCC) is greatly enhanced by the absence of the core fucose of oligosaccharides attached to the Fc, and is closely related to the clinical efficacy of anticancer activ...

    Authors: Harue Imai-Nishiya, Katsuhiro Mori, Miho Inoue, Masako Wakitani, Shigeru Iida, Kenya Shitara and Mitsuo Satoh

    Citation: BMC Biotechnology 2007 7:84

    Content type: Research article

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  16. Virus-mediated delivery of therapeutic transgenes to the inflamed colon holds a great potential to serve as an effective therapeutic strategy for inflammatory bowel disease, since local, long-term expression o...

    Authors: Samuel J Farlow, Alan Jerusalmi and Takeshi Sano

    Citation: BMC Biotechnology 2007 7:83

    Content type: Research article

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  17. DNA microarrays are among the most widely used technical platforms for DNA and RNA studies, and issues related to microarrays sensitivity and specificity are therefore of general importance in life sciences. C...

    Authors: Nicoletta Mascellani, Xiuping Liu, Simona Rossi, Jlenia Marchesini, Davide Valentini, Diego Arcelli, Cristian Taccioli, Mauro Helmer Citterich, Chang-Gong Liu, Rita Evangelisti, Giandomenico Russo, Jorge M Santos, Carlo M Croce and Stefano Volinia

    Citation: BMC Biotechnology 2007 7:82

    Content type: Methodology article

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  18. Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody ...

    Authors: Pascal Philibert, Audrey Stoessel, Wei Wang, Annie-Paule Sibler, Nicole Bec, Christian Larroque, Jeffery G Saven, Jérôme Courtête, Etienne Weiss and Pierre Martineau

    Citation: BMC Biotechnology 2007 7:81

    Content type: Research article

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  19. RSF1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. RSF1010-derived plasmid vectors are widely used in both basic research and industrial applications. In ...

    Authors: Joanna I Katashkina, Tatiana M Kuvaeva, Irina G Andreeva, Alexandra Yu Skorokhodova, Irina V Biryukova, Irina L Tokmakova, Lubov I Golubeva and Sergey V Mashko

    Citation: BMC Biotechnology 2007 7:80

    Content type: Research article

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  20. RNA polymerase III (pol III) type 3 promoters such as U6 or 7SK are commonly used to express short-hairpin RNA (shRNA) effectors for RNA interference (RNAi). To extend the use of RNAi for studies of developmen...

    Authors: Stephanie C Bannister, Terry G Wise, David M Cahill and Timothy J Doran

    Citation: BMC Biotechnology 2007 7:79

    Content type: Research article

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  21. Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and sp...

    Authors: Jinny L Liu, George P Anderson and Ellen R Goldman

    Citation: BMC Biotechnology 2007 7:78

    Content type: Research article

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  22. The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, b...

    Authors: Javier F Chaparro-Riggers, Bernard LW Loo, Karen M Polizzi, Phillip R Gibbs, Xiao-Song Tang, Mark J Nelson and Andreas S Bommarius

    Citation: BMC Biotechnology 2007 7:77

    Content type: Methodology article

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  23. Adjustable gene expression is crucial in a number of applications such as de- or transdifferentiation of cell phenotypes, tissue engineering, various production processes as well as gene-therapy initiatives. V...

    Authors: David A Fluri, Marie Daoud-El Baba and Martin Fussenegger

    Citation: BMC Biotechnology 2007 7:75

    Content type: Research article

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  24. Electrotransfer of plasmid DNA into skeletal muscle is a promising strategy for the delivery of therapeutic molecules targeting various muscular diseases, cancer and lower-limb ischemia. Internal Ribosome Entr...

    Authors: Camille Allera-Moreau, Aurélie Delluc-Clavières, Caroline Castano, Loïc Van den Berghe, Muriel Golzio, Marc Moreau, Justin Teissié, Jean-François Arnal and Anne-Catherine Prats

    Citation: BMC Biotechnology 2007 7:74

    Content type: Methodology article

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  25. The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to consti...

    Authors: Shan Goh, Andrea Camattari, Daniel Ng, Ruth Song, Kevin Madden, Janet Westpheling and Victor VT Wong

    Citation: BMC Biotechnology 2007 7:72

    Content type: Research article

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  26. Genomics tools, particularly DNA microarrays, have found application in a number of areas including gene discovery and disease characterization. Despite the vast utility of these tools, little work has been do...

    Authors: Pratik Jaluria, Michael Betenbaugh, Konstantinos Konstantopoulos and Joseph Shiloach

    Citation: BMC Biotechnology 2007 7:71

    Content type: Research article

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  27. There is much evidence that tumor cells elicit a humoral immune response in patients. In most cases, the presence of antibodies in peripheral blood is detected only in small proportion of patients with tumors ...

    Authors: Emiliano Pavoni, Giorgia Monteriù, Daniela Santapaola, Fiorella Petronzelli, Anna Maria Anastasi, Angela Pelliccia, Valeria D'Alessio, Rita De Santis and Olga Minenkova

    Citation: BMC Biotechnology 2007 7:70

    Content type: Research article

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  28. In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g. h...

    Authors: Magnus Stougaard, Jakob S Lohmann, Magdalena Zajac, Stephen Hamilton-Dutoit and Jørn Koch

    Citation: BMC Biotechnology 2007 7:69

    Content type: Methodology article

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  29. The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capabi...

    Authors: Gaetano Donofrio, Chiara Sartori, Lara Ravanetti, Sandro Cavirani, Laurent Gillet, Alain Vanderplasschen, Simone Taddei and Cesidio Filippo Flammini

    Citation: BMC Biotechnology 2007 7:68

    Content type: Research article

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  30. Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled...

    Authors: Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao and Joseph C Wu

    Citation: BMC Biotechnology 2007 7:67

    Content type: Research article

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  31. High content screening (HCS)-based image analysis is becoming an important and widely used research tool. Capitalizing this technology, ample cellular information can be extracted from the high content cellula...

    Authors: Fuhai Li, Xiaobo Zhou, Jinmin Zhu, Jinwen Ma, Xudong Huang and Stephen TC Wong

    Citation: BMC Biotechnology 2007 7:66

    Content type: Research article

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  32. Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic pha...

    Authors: Lauren RH Krumpe, Kathryn M Schumacher, James B McMahon, Lee Makowski and Toshiyuki Mori

    Citation: BMC Biotechnology 2007 7:65

    Content type: Methodology article

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  33. The growing field of proteomics and systems biology is resulting in an ever increasing demand for purified recombinant proteins for structural and functional studies. Here, we show a systematic approach to suc...

    Authors: Claudia Langlais, Birgit Guilleaume, Nadja Wermke, Tina Scheuermann, Lars Ebert, Joshua LaBaer and Bernhard Korn

    Citation: BMC Biotechnology 2007 7:64

    Content type: Research article

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  34. Plant viruses can be employed as versatile vectors for the production of vaccines by expressing immunogenic epitopes on the surface of chimeric viral particles. Although several viruses, including tobacco mosa...

    Authors: Chung-Da Yang, Jia-Teh Liao, Chen-Yen Lai, Ming-Hwa Jong, Chi-Ming Liang, Yeou-Liang Lin, Na-Sheng Lin, Yau-Heiu Hsu and Shu-Mei Liang

    Citation: BMC Biotechnology 2007 7:62

    Content type: Research article

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  35. Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be ind...

    Authors: Daniel C Gray, Klaus P Hoeflich, Li Peng, Zhenyu Gu, Alvin Gogineni, Lesley J Murray, Mike Eby, Noelyn Kljavin, Somasekar Seshagiri, Mary J Cole and David P Davis

    Citation: BMC Biotechnology 2007 7:61

    Content type: Methodology article

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  36. Microfluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practic...

    Authors: Bong Geun Chung, Jeong Won Park, Jia Sheng Hu, Carlos Huang, Edwin S Monuki and Noo Li Jeon

    Citation: BMC Biotechnology 2007 7:60

    Content type: Methodology article

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  37. A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologo...

    Authors: Mandy M Cox, Sherryll L Layton, Tieshan Jiang, Kim Cole, Billy M Hargis, Luc R Berghman, Walter G Bottje and Young Min Kwon

    Citation: BMC Biotechnology 2007 7:59

    Content type: Methodology article

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  38. Bacteriophages infecting lactic acid bacteria (LAB) are widely acknowledged as the main cause of milk fermentation failures. In this study, we describe the surface-expression as well as the secretion of two fu...

    Authors: Anna Hultberg, Denise M Tremblay, Hans de Haard, Theo Verrips, Sylvain Moineau, Lennart Hammarström and Harold Marcotte

    Citation: BMC Biotechnology 2007 7:58

    Content type: Research article

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  39. The interpretability of microarray data can be affected by sample quality. To systematically explore how RNA quality affects microarray assay performance, a set of rat liver RNA samples with a progressive chan...

    Authors: Karol L Thompson, P Scott Pine, Barry A Rosenzweig, Yaron Turpaz and Jacques Retief

    Citation: BMC Biotechnology 2007 7:57

    Content type: Research article

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  40. We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for a...

    Authors: Thomas O Kohl, Inga I Hitzeroth, Neil D Christensen and Edward P Rybicki

    Citation: BMC Biotechnology 2007 7:56

    Content type: Research article

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  41. Bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. Among them, strains of the genus Pediococcus are particularly interesting for their ability to produce pediocin, a broa...

    Authors: Sophie Mathys, Ueli von Ah, Christophe Lacroix, Ernö Staub, Raffaella Mini, Tania Cereghetti and Leo Meile

    Citation: BMC Biotechnology 2007 7:55

    Content type: Research article

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  42. Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the kinetically controlled synthesis of semi-synthetic ...

    Authors: Davide A Cecchini, Immacolata Serra, Daniela Ubiali, Marco Terreni and Alessandra M Albertini

    Citation: BMC Biotechnology 2007 7:54

    Content type: Research article

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  43. Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and ...

    Authors: Mahiran Basri, Raja Noor Zaliha Raja Abd Rahman, Afshin Ebrahimpour, Abu Bakar Salleh, Erin Ryantin Gunawan and Mohd Basyaruddin Abd Rahman

    Citation: BMC Biotechnology 2007 7:53

    Content type: Research article

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  44. The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising im...

    Authors: Li-Te Chin, Chishih Chu, Han-Min Chen, Shu-Ching Hsu, Bor-Chun Weng and Chi-Hong Chu

    Citation: BMC Biotechnology 2007 7:51

    Content type: Research article

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