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  1. Content type: Research article

    As a very important coenzyme in the cell metabolism, Vitamin B12 (cobalamin, VB12) has been widely used in food and medicine fields. The complete biosynthesis of VB12 requires approximately 30 genes, but overexpr...

    Authors: Yingying Cai, Miaomiao Xia, Huina Dong, Yuan Qian, Tongcun Zhang, Beiwei Zhu, Jinchuan Wu and Dawei Zhang

    Citation: BMC Biotechnology 2018 18:27

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  2. Content type: Research article

    Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restr...

    Authors: Xuesong Zhang, Shiyong Liao, Fuliang Cao, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:26

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  3. Content type: Research article

    Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive ...

    Authors: Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang and Kunzhi Jia

    Citation: BMC Biotechnology 2018 18:25

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  4. Content type: Research article

    Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the be...

    Authors: Huidan Zhang, Dong Lu, Xin Li, Yingang Feng, Qiu Cui and Xiaojin Song

    Citation: BMC Biotechnology 2018 18:23

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  5. Content type: Research article

    The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and in...

    Authors: Xiaoyue Zhang, Keyan Xu, Yanmei Ou, Xiaodong Xu and Hongying Chen

    Citation: BMC Biotechnology 2018 18:24

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  6. Content type: Research article

    Violacein is a deep violet compound that is produced by a number of bacterial species. It is synthesized from tryptophan by a pathway that involves the sequential action of 5 different enzymes (encoded by genes v...

    Authors: Elizabeth Bilsland, Tatyana A. Tavella, Renata Krogh, Jamie E. Stokes, Annabelle Roberts, James Ajioka, David R. Spring, Adriano D. Andricopulo, Fabio T. M. Costa and Stephen G. Oliver

    Citation: BMC Biotechnology 2018 18:22

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  7. Content type: Research article

    Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin’s N-terminus must engage thrombin’s active site for effective inhibition, fusing a ...

    Authors: William P. Sheffield, Louise J. Eltringham-Smith and Varsha Bhakta

    Citation: BMC Biotechnology 2018 18:21

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  8. Content type: Methodology article

    Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these...

    Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero and Marco A. Ramos

    Citation: BMC Biotechnology 2018 18:20

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  9. Content type: Research article

    Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal...

    Authors: Tomoe Kobayashi, Masumi Namba, Takayuki Koyano, Masaki Fukushima, Masahiro Sato, Masato Ohtsuka and Makoto Matsuyama

    Citation: BMC Biotechnology 2018 18:19

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  10. Content type: Research article

    Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high...

    Authors: Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa and Chiara Schiraldi

    Citation: BMC Biotechnology 2018 18:18

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  11. Content type: Methodology article

    We demonstrate the feasibility of creating a pair of reference samples to be used as surrogates for clinical samples measured in either a research or clinical laboratory setting. The reference sample paradigm ...

    Authors: P. Scott Pine, Steven P. Lund, Sanford A. Stass, Debra Kukuruga, Feng Jiang, Lynn Sorbara, Sudhir Srivastava and Marc Salit

    Citation: BMC Biotechnology 2018 18:17

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  12. Content type: Research article

    microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, ...

    Authors: Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson and Peter J. Leedman

    Citation: BMC Biotechnology 2018 18:16

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  13. Content type: Research article

    Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). Proof of concept studies have demonstrate...

    Authors: Christian A. Schaer, Catherine Owczarek, Jeremy W. Deuel, Stefan Schauer, Jin Hyen Baek, Ayla Yalamanoglu, Matthew P. Hardy, Pierre D. Scotney, Peter M. Schmidt, Matthias Pelzing, Peter Soupourmas, Paul W. Buehler and Dominik J. Schaer

    Citation: BMC Biotechnology 2018 18:15

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  14. Content type: Research article

    Dedifferentiation of chondrocytes during cell expansion is one of the barriers in tissue construction for cartilage repair. To understand chondrocyte behavior and improve cell expansion in monolayer culture, t...

    Authors: Sopita Wongin, Saranatra Waikakul, Pojchong Chotiyarnwong, Wanwipa Siriwatwechakul, Masahiro Kino-oka, Mee-Hae Kim and Kwanchanok Viravaidya-Pasuwat

    Citation: BMC Biotechnology 2018 18:14

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  15. Content type: Research article

    Filamentous bacteria of the genus Streptomyces produce a large arsenal of industrially relevant antibiotics and enzymes. The industrial production of these molecules occurs in large fermenters, where many strepto...

    Authors: Boris Zacchetti, Agathoklis Andrianos, Dino van Dissel, Evelien de Ruiter, Gilles P. van Wezel and Dennis Claessen

    Citation: BMC Biotechnology 2018 18:13

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  16. Content type: Research article

    Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding f...

    Authors: Shasha Yang, Sili Wei, Yun Mao, Hanxue Zheng, Juantao Feng, Jihong Cui, Xin Xie, Fulin Chen and Honmgmin Li

    Citation: BMC Biotechnology 2018 18:12

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  17. Content type: Research article

    Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression o...

    Authors: Paola Mini, Olivia Costantina Demurtas, Silvia Valentini, Patrizia Pallara, Giuseppe Aprea, Paola Ferrante and Giovanni Giuliano

    Citation: BMC Biotechnology 2018 18:11

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  18. Content type: Research article

    The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca2+-binding moiety inserted into the mNeonGr...

    Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov and F. V. Subach

    Citation: BMC Biotechnology 2018 18:10

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  19. Content type: Research article

    The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and...

    Authors: Anil S. Prajapati, Vishakha A. Pawar, Ketankumar J. Panchal, Ankit P. Sudhir, Bhaumik R. Dave, Darshan H. Patel and R. B. Subramanian

    Citation: BMC Biotechnology 2018 18:9

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  20. Content type: Methodology article

    Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioi...

    Authors: Christoph Geisler

    Citation: BMC Biotechnology 2018 18:8

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  21. Content type: Methodology article

    Detection of DNA methylome at single-base resolution is a significant challenge but promises to shed considerable light on human disease etiology. Current technologies could not detect DNA methylation genome-w...

    Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu

    Citation: BMC Biotechnology 2018 18:7

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  22. Content type: Methodology article

    Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate l...

    Authors: Russell D. Wolfinger, Sudheer Beedanagari, Eric Boitier, Tao Chen, Philippe Couttet, Heidrun Ellinger-Ziegelbauer, Gregory Guillemain, Claire Mariet, Peter Mouritzen, Raegan O’Lone, P. Scott Pine, Tatiana Sharapova, Jian Yan, Peter S. Yuen and Karol L. Thompson

    Citation: BMC Biotechnology 2018 18:6

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  23. Content type: Methodology article

    Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the...

    Authors: Zhang Zhang, Shunfang Qiu, Xiaopeng Zhang and Wei Chen

    Citation: BMC Biotechnology 2018 18:4

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  24. Content type: Research article

    L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional products. In this study, a system level engineering was conducted to enhance L-Phe biosy...

    Authors: Yongfei Liu, Yiran Xu, Dongqin Ding, Jianping Wen, Beiwei Zhu and Dawei Zhang

    Citation: BMC Biotechnology 2018 18:5

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  25. Content type: Methodology article

    RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such...

    Authors: Po-Hao Wang, Greg Schulenberg, Shannon Whitlock, Andrew Worden, Ning Zhou, Stephen Novak and Wei Chen

    Citation: BMC Biotechnology 2018 18:3

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  26. Content type: Research article

    The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce ...

    Authors: Agnieszka J. Turlo, Yalda Ashraf Kharaz, Peter D. Clegg, James Anderson and Mandy J. Peffers

    Citation: BMC Biotechnology 2018 18:2

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  27. Content type: Research article

    The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) fo...

    Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Elisabeth Riegel, Michaela Aigner, Holger Bock, Birgit Werner, Thomas Lindhorst and Thomas Czerny

    Citation: BMC Biotechnology 2018 18:1

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  28. Content type: Research article

    Massively parallel genetic sequencing allows rapid testing of known intellectual disability (ID) genes. However, the discovery of novel syndromic ID genes requires molecular confirmation in at least a second o...

    Authors: Tracy Dudding-Byth, Anne Baxter, Elizabeth G. Holliday, Anna Hackett, Sheridan O’Donnell, Susan M. White, John Attia, Han Brunner, Bert de Vries, David Koolen, Tjitske Kleefstra, Seshika Ratwatte, Carlos Riveros, Steve Brain and Brian C. Lovell

    Citation: BMC Biotechnology 2017 17:90

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  29. Content type: Methodology article

    Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning meth...

    Authors: Jacob J. Messner, Honor L. Glenn and Deirdre R. Meldrum

    Citation: BMC Biotechnology 2017 17:89

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  30. Content type: Research article

    Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst

    Authors: Erick Abreu Silveira, Sonia Moreno-Perez, Alessandra Basso, Simona Serban, Rita Pestana Mamede, Paulo W. Tardioli, Cristiane Sanchez Farinas, Javier Rocha-Martin, Gloria Fernandez-Lorente and Jose M. Guisan

    Citation: BMC Biotechnology 2017 17:88

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  31. Content type: Research article

    Starch-binding domains from carbohydrate binding module family 20 have been used as a tool for starch engineering. Previous studies showed that

    Authors: Xing-Feng Huang, Farhad Nazarian, Jean-Paul Vincken, Richard G. F. Visser and Luisa M. Trindade

    Citation: BMC Biotechnology 2017 17:86

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  32. Content type: Research article

    Receptor activator of NF-κB ligand (RANKL)/RANK signaling essentially functions within the skeletal system, particularly participating in osteoclastogenesis and bone resorption. In addition, this signaling pat...

    Authors: Yilei Wei, Yu Zhan, Pengfei Chen, Zhi Liu, Haohao Zhang, Dandan Liu, Jie Zhang, Min Yu, Wei Mo, Jun Zhang and Xiaoren Zhang

    Citation: BMC Biotechnology 2017 17:87

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  33. Content type: Research article

    When the unavoidable waste generation is considered as damaging to our environment, it becomes crucial to develop a sustainable technology to remediate the pollutant source towards an environmental protection ...

    Authors: Arezoo Dadrasnia, Mohd Sofian Azirun and Salmah Binti Ismail

    Citation: BMC Biotechnology 2017 17:85

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  34. Content type: Research article

    The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluat...

    Authors: Zhen Chen, Zhipeng Li, Peize Liu, Yu Liu, Yuanpeng Wang, Qingbiao Li and Ning He

    Citation: BMC Biotechnology 2017 17:84

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  35. Content type: Research article

    Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4], [Cl], [MeCO2]) were...

    Authors: Zhao Wang, John Gräsvik, Leif J. Jönsson and Sandra Winestrand

    Citation: BMC Biotechnology 2017 17:82

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  36. Content type: Methodology article

    Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembl...

    Authors: Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong and Yibin Lin

    Citation: BMC Biotechnology 2017 17:81

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  37. Content type: Methodology article

    There is a need for the development of synthetic biology methods and tools to facilitate rapid and efficient engineering of yeast that accommodates the needs of specific biotechnology projects. In particular, ...

    Authors: Ana Pérez-González, Ryan Kniewel, Marcel Veldhuizen, Hemant K. Verma, Mónica Navarro-Rodríguez, Luis M. Rubio and Elena Caro

    Citation: BMC Biotechnology 2017 17:80

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  38. Content type: Research article

    The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus ...

    Authors: Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J. Stonehouse…

    Citation: BMC Biotechnology 2017 17:79

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  39. Content type: Research article

    Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). ...

    Authors: Mary Ladidi Abu, Hisham Mohd Nooh, Siti Nurbaya Oslan and Abu Bakar Salleh

    Citation: BMC Biotechnology 2017 17:78

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  40. Content type: Research article

    Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antiba...

    Authors: Natalia Inés Almasia, María Paula Molinari, Guillermo Andrés Maroniche, Vanesa Nahirñak, María Pilar Barrios Barón, Oscar Alberto Taboga and Cecilia Vazquez Rovere

    Citation: BMC Biotechnology 2017 17:75

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  41. Content type: Research article

    Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits a highly restricted tropism for cells of the monocyte-macrophage lineage, utilizing porcine CD163 (pCD163) as an indispensable cellular rece...

    Authors: Liangliang Li, Chunyan Wu, Gaopeng Hou, Biyun Xue, Sha Xie, Qin Zhao, Yuchen Nan, Gaiping Zhang and En-Min Zhou

    Citation: BMC Biotechnology 2017 17:77

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  42. Content type: Research article

    The myxomycetes derive their common name (slime molds) from the multinucleate trophic stage (plasmodium) in the life cycle, which typically produces a noticeable amount of slimy materials, some of which is nor...

    Authors: Tuyen T.M Huynh, Trung V. Phung, Steven L. Stephenson and Hanh T.M Tran

    Citation: BMC Biotechnology 2017 17:76

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  43. Content type: Research article

    Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and ...

    Authors: Rong Li, Zhenzhen Yan, Juan Wang, Qisheng Song and Zhi Wang

    Citation: BMC Biotechnology 2017 17:73

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  44. Content type: Research article

    β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the te...

    Authors: Fei Chang, Xianbing Zhang, Yu Pan, Youxue Lu, Wei Fang, Zemin Fang and Yazhong Xiao

    Citation: BMC Biotechnology 2017 17:74

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  45. Content type: Research article

    α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent n...

    Authors: Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang and Danke Xu

    Citation: BMC Biotechnology 2017 17:72

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  46. Content type: Research article

    Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards t...

    Authors: Beatriz Temer, Leandro Vieira dos Santos, Victor Augusti Negri, Juliana Pimentel Galhardo, Pedro Henrique Mello Magalhães, Juliana José, Cidnei Marschalk, Thamy Lívia Ribeiro Corrêa, Marcelo Falsarella Carazzolle and Gonçalo Amarante Guimarães Pereira

    Citation: BMC Biotechnology 2017 17:71

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  47. Content type: Research article

    Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed...

    Authors: Aldricho Alpha Pollardo, Hong-shik Lee, Dohoon Lee, Sangyong Kim and Jaehoon Kim

    Citation: BMC Biotechnology 2017 17:70

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  48. Content type: Research article

    Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen ...

    Authors: Huidan Zhang, Yingang Feng, Qiu Cui and Xiaojin Song

    Citation: BMC Biotechnology 2017 17:68

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