Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
© Karan et al.; licensee BioMed Central Ltd. 2013
Received: 3 October 2012
Accepted: 14 January 2013
Published: 16 January 2013
Halorubrum lacusprofundi is a cold-adapted halophilic archaeon isolated from Deep Lake, a perennially cold and hypersaline lake in Antarctica. Its genome sequencing project was recently completed, providing access to many genes predicted to encode polyextremophilic enzymes active in both extremely high salinity and cold temperatures.
Analysis of the genome sequence of H. lacusprofundi showed a gene cluster for carbohydrate utilization containing a glycoside hydrolase family 42 β-galactosidase gene, named bga. In order to study the biochemical properties of the β-galactosidase enzyme, the bga gene was PCR amplified, cloned, and expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter. The recombinant β-galactosidase protein was produced at 20-fold higher levels compared to H. lacusprofundi, purified using gel filtration and hydrophobic interaction chromatography, and identified by SDS-PAGE, LC-MS/MS, and ONPG hydrolysis activity. The purified enzyme was found to be active over a wide temperature range (−5 to 60°C) with an optimum of 50°C, and 10% of its maximum activity at 4°C. The enzyme also exhibited extremely halophilic character, with maximal activity in either 4 M NaCl or KCl. The polyextremophilic β-galactosidase was also stable and active in 10–20% alcohol-aqueous solutions, containing methanol, ethanol, n-butanol, or isoamyl alcohol.
The H. lacusprofundi β-galactosidase is a polyextremophilic enzyme active in high salt concentrations and low and high temperature. The enzyme is also active in aqueous-organic mixed solvents, with potential applications in synthetic chemistry. H. lacuprofundi proteins represent a significant biotechnology resource and for developing insights into enzyme catalysis under water limiting conditions. This study provides a system for better understanding how H. lacusprofundi is successful in a perennially cold, hypersaline environment, with relevance to astrobiology.
KeywordsPolyextremophiles Extremozymes Protein stability Halophiles Psychrophiles Biofuels
The genome sequence of a cold-adapted extremely halophilic archaeon, Halorubrum lacusprofundi, was recently completed and analyzed by comparative genomics [1–3]. Like other halophilic Archaea, H. lacusprofundi has the ability to grow and thrive in salt-rich environments and its metabolic processes and physiological functions prevail under nearly saturating salt conditions similar to those found in their natural hypersaline environment (21–28%, w/v salt content) [4–6]. Among halophiles, H. lacusprofundi is distinguished by survival in a perennially cold habitat, Deep Lake, Antarctica, where the normal temperature is in the range from +11.5°C to −18°C. The lake brine remains liquid throughout the year as a result of freezing point depression from the extremely high salinity. Due to its success in such an unusually harsh environment, H. lacusprofundi has been of significant microbiological, biotechnological, and astrobiological interest [5, 7–9].
With the release of the H. lacusprofundi genome sequence, we identified the bga gene coding a glycoside hydrolase. The bga gene product is a putative β-galactosidase (E.C. 220.127.116.11), well-known to hydrolyze lactose into glucose and galactose. Broadly, β-galactosidases are classified into seven glycoside hydrolase (GH) families based on functional similarities , with the extensively studied E. coli enzyme belonging to the GH-2 family . Extremophilic β-galactosidases from cold-adapted, halophilic, and thermophilic species are members primarily of two families, either the GH-2 or GH-42 family [12–17]. The H. lacusprofundi β-galactosidase has been classified as a member of the GH-42 family .
The H. lacusprofundi β-galactosidase enzyme was of interest as a result of its expected “polyextremophilic” character with predicted activity at both high salt concentrations and extreme temperatures. Such polyextremophilic enzymes may have novel biotechnological applications, for example in synthetic chemistry, where they can be active and stable in the presence of organic solvents due to tight binding of water . Organic solvents in reaction mixtures increase the solubility of hydrophobic substrates, and have the potential to improve the kinetic equilibrium and increase the yield and specificity of the product . However, organic solvents generally disrupt hydrophobic interactions within enzymes, causing them to lose their catalytic activity. High salt solutions and low temperatures mimic non-aqueous environments, since water activity is reduced and enzymes must successfully compete for available water for function .
In the present study, we have purified and characterized the GH-42 family β-galactosidase from the cold-adapted haloarchaeon, H. lacusprofundi, after overexpression of the gene in the model haloarchaeon, Halobacterium sp. NRC-1. Our results show that the enzyme is active at high salinity and wide temperature range, and functions in the presence of a number of organic solvents.
Restriction enzymes, T4 DNA ligase, and Taq DNA polymerase were purchased from New England Biolabs (Beverly, MA, USA). X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) and ONPG (o-nitrophenyl-β-D-galactopyranoside) were purchased from IBI Scientific (Peosta, Iowa, USA) and USB Corporation (Cleveland, OH, USA). All other chemicals were purchased from Sigma (St. Louis, MO, USA).
Strains, media, and culture conditions
Halorubrum lacusprofundi isolated from Deep Lake, Antarctica  was obtained from the American Type Culture Collection (ATCC) (strain ATCC 49239). It was grown in ATCC medium 1682, artificial Deep Lake medium, prepared according to the directions from ATCC at 30°C with shaking. Escherichia coli DH5α was grown at 37°C in Luria-Bertani (LB) medium supplemented with 100 μg/ml ampicillin. Halobacterium sp. strain NRC-1 and derivatives were cultured in CM+ medium containing 4.3 M NaCl and trace metals at 42°C with shaking as previously described [24, 25]. For solid media, 2% (w/v) agar was added, and when required, 5-bromo-4-chloro-indolyl-β-D-galactopyranoside (X-Gal) was added to 40 μg/ml. Stock cultures were maintained in glycerol at −80°C. For short-term use, purified cultures were maintained on stock plates at 4°C.
Measurement of β-galactosidase activity
Cells were harvested by centrifugation (6,000 × g, 4°C, 10 min) in a Sorvall RC-5B centrifuge and disrupted in 50 mM Tris-buffer, pH 8.0 (containing 2 M KCl, 10% v/v glycerol, 0.1 mM ZnCl2, 50 mM MgCl2, 1 mM EDTA, and 100 μg PMSF/ml) using a sonicator (Model CL-18, Fisher Scientific, USA). Cell debris was removed by centrifugation (25,000 × g, 4°C, 10 min) in an Eppendorf 5417C centrifuge to obtain the crude extract and analyzed for β-galactosidase activity. Enzymatic activity was carried out for 10 minutes at 25°C and pH 6.5 using 2.2 mM of the synthetic chromogenic substrate o-nitrophenyl β-D galactopyranoside (ONGP) as a substrate and stopped by the addition of Na2CO3 to 1.0 M concentration [18, 26]. The o-nitrophenol released from ONPG by β-galactosidase was measured at 420 nm using a UV-1601 spectrophotometer (Shimadzu, Kyoto, Japan). One international unit (IU) of β-galactosidase activity is defined as the amount of enzyme liberating 1 μmol of o-nitrophenol per minute. Miller units (MU) are calculated as 1,000 × OD420 / (t × V × OD600), where t is the reaction time in minutes, V is the volume of cells used in milliliters and cell density is measured at OD600.
Bioinformatic analysis of the H. lacusprofundi genome was conducted using tools on the Carbohydrate Active Enzymes (CAZY) database (http://www.cazy.org) and the HaloWeb site (http://halo4.umbi.umd.edu) [10, 28]. The HaloWeb site provided access to the H. lacusprofundi genome information, including genetic maps, gene functions, DNA and protein sequences, and haloarchaeal orthologous groups (HOGs) [2, 10, 28]. The β-galactosidase gene (Hlac_2868) and surrounding genes were further analyzed using NCBI clusters of orthologous groups (COGs) . Similar proteins were also identified by BlastP analysis using the H. lacusprofundi predicted protein sequence as query and downloaded for local analysis from NCBI [30, 31]. The CAZY database provided the assignment of the H. lacusprofundi β-galactosidase protein as a glycosyl hydrolase family 42 member and links to additional information, including homologous enzymes and structures. Additional phylogenetic analysis of β-galactosidase protein sequences was performed using ClustalX .
Construction of the β-galactosidase gene expression plasmid
Oligonucleotides used in this study as primers for PCR
Amplification of cspD2 promoter
Amplification of bga gene
Sequencing of bga gene
β-gal 15 F
β-gal 04 F
Sequencing of cspD2 promoter in pMC2 plasmid
Expression of the β-galactosidase gene in Halobacteriumsp. NRC-1
Halobacterium sp. NRC-1, which does not possess an endogenous bga gene, was transformed with pMC2, using the EDTA-PEG method  and transformants were selected on CM+ agar plates supplemented with 20 μg/ml mevinolin. Transformants were either grown to late log phase (OD600 of 0.9–1.0) at 42°C in CM+ medium supplemented with 20 μg/ml mevinolin or streaked on CM+ plates containing 40 μg/ml X-Gal and 20 μg/ml mevinolin. To induce β-galactosidase production, the cultures were further incubated at various temperatures for 72 h.
Crude cell extract (2 ml), prepared as described above, was subjected to gel filtration chromatography on a Sephadex G-200 (Pharmacia Fine Chemicals, Sweden) column (2.5 cm × 50 cm) equilibrated with 50 mM sodium phosphate buffer, pH 7.0, containing 1.25 M (NH4)2SO4 and 1.25 M KCl. Fractions of 2.0 ml were collected at a flow rate of 0.35 ml/min using a fraction collector (Bio-Rad Laboratories, Hercules, CA) using the same buffer. Protein content (Abs 280 nm) and β-galactosidase activity were determined for collected fractions. The active fractions obtained by gel filtration were combined and further purified by hydrophobic interaction chromatography (HIC) on a Phenyl Sepharose 6 Fast Flow (Sigma, St. Louis, MO, USA) column (1.5 cm × 50 cm), equilibrated with 50 mM sodium phosphate buffer, pH 7.0 containing 1.25 M (NH4)2SO4 and 1.25 M KCl. After loading 25 ml of sample, the column was washed with the same buffer until unbound proteins were removed. The bound proteins were eluted with a decreasing gradient (100–0%) of 50 mM sodium phosphate buffer, pH 7.0, containing 1.25 M (NH4)2SO4 and 1.25 M KCl and increasing gradient (0–100%) of 50 mM sodium phosphate buffer, pH 7.0. Fractions (2.0 ml each) were collected at a flow rate of 0.35 ml/min using a fraction collector and analyzed for protein concentration and β-galactosidase activity. Protein concentrations were determined by the Bradford dye binding method  using bovine serum albumin as a standard. During chromatographic purification steps, protein concentration was estimated by recording the absorbance at 280 nm with a BioLogic LP system (Bio-Rad Laboratories, Hercules, CA).
Polyacrylamide gel electrophoresis
Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was carried out according to the method of Laemmli  using 8% (29:1 acrylamide to bis-acrylamide) cross-linked polyacrylamide gels on a vertical gel electrophoresis unit (Bio-Rad Laboratories). The gels were stained with 0.1% Coomassie blue R-250 (in methanol–acetic acid–water, 40:10:50, v/v) followed by destaining with methanol–acetic acid–water (40:10:50, v/v).
Identification of purified protein by LC-MS/MS analysis
To facilitate identification of purified protein by LC-MS/MS analysis, the Coomassie-stained protein band was excised and disulfide bonds were reduced with tris(hydroxypropyl)phosphine (TCEP). Next, the protein was digested with trypsin and peptides were separated by nanoscale reverse-phase liquid chromatography using an Xtreme Simple nanoLC system (CVC/Micro-Tech, Fontana, CA). A LTQ-Orbitrap mass spectrometer (Thermo Electron, Waltham, MA) equipped with a nanospray ionization source was used for data generation. MS/MS spectra were searched against protein databases using Sorcerer™-SEQUEST® (Sage-N Research, Milpitas, CA). The quality of peptide and protein assignments was assessed using PeptideProphet and ProteinProphet .
Effect of salt, pH, and temperature on β-galactosidase activity
The effect of NaCl/KCl concentration on β-galactosidase activity was evaluated in the presence of 0–4.5 M NaCl/KCl in the enzyme reaction mixture. The effect of pH was evaluated by assaying β-galactosidase activity in 50 mM sodium phosphate (pH 6.0–7.0) or Tris–HCl (pH 7.5–8.0) buffers. A plot of relative activity against pH was created to determine the optimum pH for the reaction. To determine the optimum temperature, the activity of β-galactosidase was measured at various temperatures. The percentage of maximal activity was calculated by considering the maximum activity under the given conditions as 100%.
Effect of organic solvents on the activity and stability of β-galactosidase
To determine the effect of organic solvents on β-galactosidase activity, enzyme assays were performed in the absence and presence of organic solvents (5 and 10%, v/v methanol, ethanol, n-butanol, and isoamyl alcohol). For the stability of the purified β-galactosidase in aqueous-alcohol solutions, enzyme was pre-incubated at 30°C with constant shaking at 200 rpm for 3 h in the absence or presence of organic solvent (10 and 20%, v/v). Samples were taken at different time intervals and the residual enzyme activity was determined as described above.
H. lacusprofundiβ-galactosidase gene, protein, and enzyme activity
Cloning and overexpression of H. lacusprofundi β-galactosidase gene in Halobacteriumsp. NRC-1
In order to study the H. lacusprofundi β-galactosidase in more detail, we cloned and overexpressed the bga gene in a genetically tractable haloarchaeal host, Halobacterium sp. NRC-1, which lacks an endogenous β-galactosidase . The expression plasmid, pMC2, contained the β-lactamase gene (bla) for selection of ampicillin resistance in E. coli, HMG-CoA reductase gene (mev) for selection of mevinolin resistance in Halobacterium sp. NRC-1, and origins of replication for both E. coli (ColE1 plasmid) and Halobacterium sp. NRC-1 (pGRB plasmid) (Figure 2) [39, 40]. Plasmid pMC2 was transformed into Halobacterium sp. NRC-1, and plated on CM+ agar plates containing X-gal and mevinolin. β-galactosidase enzyme activity was determined by the appearance of blue NRC-1 colonies on agar plates (data not shown). The Halobacterium sp. NRC-1(pMC2) strain was grown in liquid culture, lysed, and crude lysate assayed for β-galactosidase activity (Figure 3). The results clearly demonstrated that the recombinant Halobacterium sp. NRC-1(pMC2) strain produces high levels of β-galactosidase, nearly 20-fold higher than wild-type H. lacusprofundi (Figure 3). A nearly identical temperature profile was observed for the enzyme produced in both H. lacusprofundi and Halobacterium sp. NRC-1(pMC2), with activity from −5°C to 60°C.
Purification and identification of H. lacusprofundiβ-galactosidase
Purification of H. lacusprofundi β-galactosidase
Total protein (mg)
Total activity (IU)
Specific activity (units/mg protein)
Hydrophobic interaction chromatography
Unique peptides identified by LC-MS/MS analysis
Amino acid sequence
Number of times peptide identified
Expected mass value (Da)
Query coverage* (%) to H. lacusprofundiβ-galactosidase
Identity with H. lacusprofundiβ-galactosidase
Characterization of purified H. lacusprofundiβ-galactosidase
Effect of organic solvents on the activity and stability of β-galactosidase
We have established that the glycoside hydrolase GH-42 family bga gene in the cold-adapted Antarctic haloarchaeon H. lacusprofundi produces a β-galactosidase protein that is polyextremophilic. In order to characterize the salient properties of this novel enzyme, we developed a cold-inducible, cold shock protein cspD2 gene promoter-based expression plasmid in the genetic model system, Halobacterium sp. NRC-1, and overexpressed the H. lacusprofundi bga gene. A high level of active β-galactosidase protein was produced in Halobacterium sp. NRC-1 and purified by gel filtration and hydrophobic interaction chromatography, and its identity was established by LC-MS/MS, SDS-PAGE, and ONPG hydrolysis. We found that the β-galactosidase enzyme was overexpressed 20-fold, and displayed very similar properties, with optimal activity at nearly saturated concentration of salts, 4 M NaCl or KCl, and significant measurable activity at low and even subzero temperatures, as well as temperatures above 50°C. Interestingly, we also found that the enzyme was active in the presence of 10–20% organic solvents, including methanol, ethanol, n-butanol, and isoamyl alcohol. All together, these findings show a remarkable β-galactosidase displaying enzyme activity at multiple extreme conditions, with significant potential for biotechnological applications [8, 42–45]. The enzyme also serves as an excellent model for potential enzymatic activity in extraterrestrial conditions, such as those found on Mars .
The H. lacusprofundi β-galactosidase is one of few polyextremophilic enzymes to be purified and studied in detail . In the past, a barrier to such studies has been the requirement of high salt concentrations to obtain enzyme activity during overexpression in a foreign host, since low ionic strength conditions generally lead to misfolding or inactivation [8, 47]. To avoid problems overproducing active H. lacusprofundi β-galactosidase in common non-halophilic hosts such as E. coli, we chose the haloarchaeal host, Halobacterium sp. NRC-1, for overexpression. This was anticipated to be an optimal host due to its high internal salt concentration, viability at low temperatures, completely sequenced genome, lack of endogenous β-galactosidase, and many available microbiological and molecular genetic tools [12, 24, 25, 38]. In order to maximize expression of the cold-active β-galactosidase in Halobacterium sp. NRC-1, we introduced a cold-active promoter for the cold shock protein gene, cspD2, into a haloarchaeal expression vector . The cspD2 gene was selected based on previous transcriptomic studies of Halobacterium sp. NRC-1 . The combination of high salinity and low temperature induction in NRC-1 led to the successful programmed production of high amounts of active β-galactosidase, nearly 20-fold more than in its natural host.
Another challenge in studies of haloarchaeal proteins has been the development of a purification method, as a result of interference of many analytical and chromatographic techniques by high salinity levels . For purification of the H. lacusprofundi enzyme overexpressed in Halobacterium sp. NRC-1, we used a combination of ion exclusion chromatography and hydrophobic interaction chromatography, since both methods are distinguished by their ability to be applied at high salinity. It has been observed that proteins with hydrophobic “patches” on their surface tend to bind hydrophobic matrixes, a process that is facilitated by high salt concentrations [48, 49]. Similarly, ion exclusion chromatography has been successful over a wide range of ionic strength buffers, even those at high salinity . In the past, ion-exchange chromatography was also used for purification of a mesophilic halophilic β-galactosidase from the haloarchaeon, Haloferax alicantei; however, the temperature profile for this enzyme was not reported .
For the H. lacusprofundi β-galactosidase, purity was confirmed by the presence of a highly prominent band on SDS-PAGE, and its identity was verified by LC-MS/MS analysis and enzymatic breakdown of the chromogenic substrates. As previously observed for highly acidic haloarchaeal proteins, anomalous migration was expected during electrophoreses in SDS-PAGE gels, because of the binding of detergents with electrostatic and hydrophobic interactions slows the rate of migration [41, 51]. Consequently, the bga polypeptide displayed an anomalous molecular mass of ca. 100 kDa, about 28% higher than the predicted molecular mass of 78.06 kDa. However, the protein identity was validated by LC-MS/MS, with 13 peptides covering 14% of the predicted amino acid sequence. The breakdown of the chromogenic substrates, X-gal on agar plates by Halobacterium sp. NRC-1(pMC2) colonies, and ONPG by purified enzyme in solution, confirmed that the β-galactosidase was enzymatically active.
The purified H. lacusprofundi β-galactosidase was found to be extremely halophilic and retained partial activity at cold temperature and surprisingly also at elevated temperature. It exhibited maximal activity in the presence of 4.0 M NaCl/KCl, which are similar to the intracellular ionic composition observed in other haloarchaea [24, 52]. Halophilic enzymes usually feature an increase in the number of charged amino acids, especially acidic residues at the protein surface and the negative surface charge is critical to their solubility and prevents aggregation at high salt concentrations [8, 37, 47]. Although the temperature optimum was 50°C for both crude extracts and purified β-galactosidase from Halobacterium sp. NRC-1 (pMC2), the relative enzyme activity at 60°C was slightly higher for the crude extract. A reason for the observed difference could be that the purified enzyme was used without prior addition of stabilizer. The purified β-galactosidase showed a substantial fraction of activity, nearly 13% at 10°C and 10%, at 4°C. Similar temperature characteristics have been previously reported for other cold-active family 42 β-galactosidases from Arthrobacter sp. 32c  and Carnobacterium sp. BA , indicating that extremophilic enzymes frequently function suboptimally under physiological conditions. The pH optimum of β-galactosidase was near neutral, similar to other family 42 β-galactosidases [16, 18, 26] and in contrast to family 2 β-galactosidases, which are optimally active in alkaline conditions [13, 54–56].
In general, non-halophilic enzymes lose most of their activity in the presence of organic solvents . Karan et al.  have recently reported that commercial enzymes lose a significant fraction of activity under similar conditions. The H. lacusprofundi β-galactosidase, in contrast, was found to be remarkably active and stable in aqueous organic solvent mixtures. In previous work, another cold-adapted β-galactosidase from Antarctic bacterium Arthrobacter sp. 32c was also found to be active in similar ethanol concentrations (≤ 20%) . A protease from halophilic archaeon Natrialba magadii was found to be active and stable in aqueous-organic solvent mixtures containing 1.5 M NaCl and dioxane . In other studies, halophilic enzymes have been reported to be active and stable in biphasic solutions of water and hydrocarbon organic solvents, such as benzene. These include an amylase of a haloarchaeon, Haloarcula sp. strain S-1 , and a protease from the halophilic bacterium, Geomicrobium sp. EMB2 . These studies indicate that organic solvent stability is a general property of halophilic enzymes, owing to their ability to work at low water activity. However, this is the first report of retention of high levels of enzyme activity in short and long chain alcohols, which reflect the polyextremophilic character of the enzyme.
Polyextremophilic characteristics make the H. lacusprofundi β-galactosidase an ideal candidate for industrial and biotechnological uses. For example, the solvent stability of H. lacusprofundi β-galactosidase can be utilized for synthesis of oligosaccharides in a similar manner to past studies, but with the added benefit of cold activity and halophilicity. Maugard et al.  have exploited a solvent stable β-galactosidase for the synthesis of galacto-oligosaccharides from lactose. Recently, Bridiau et al.  reported a tert-butanol stable β-galactosidase from Bacillus circulans that synthesized N-acetyl-lactosamine in hydro-organic media. Vic et al.  have also reported the synthesis of 2-hydroxybenzyl β-D-glucopyranoside using β-galactosidase in a tert-butanol-water mixture.
The H. lacusprofundi β-galactosidase gene is located in a genomic region encoding proteins for binding, uptake, and catabolism of sugars. Since the environment of Deep Lake does not contain lactose, the β-galactosidase gene and surrounding gene cluster are likely to be involved in degradation and utilization of other carbohydrates, such as plant oligo- and polysaccharides . These genes reflect a substantial resource for directing the conversion of biomass into valuable commodities, such as biofuels. The properties described for the purified β-galactosidase are likely to be useful for the development and use of haloarchaea in biotechnology. Moreover, our ability to genetically manipulate and shuttle these and other genes represents a substantial resource for the future.
Halophilic Archaea offer an incomparable resource of polyextremozymes which are active and stable in high concentrations of salt, a broad range of temperatures, and organic solvents. At high salinity, water is sequestered in hydrated ionic structures, limiting the availability of free water molecules for protein hydration . Since halophilic enzymes are adapted to function at high salt concentrations, they are also found to be active at low water availability. Such conditions are also encountered by enzymes in cold temperatures due to the freezing of water molecules and consequent formation of structured ice-like lattices . Therefore, structure-function studies of cold-active haloarchaeal enzyme, including H. lacusprofundi β-galactosidase, are likely to provide further insights into enzyme catalysis under water limiting conditions, which are likely to enhance their applications in biotechnology.
Since H. lacusprofundi survives in the Antarctic Deep Lake, one of the coldest and most extremely saline environments from which microbes have been cultured, the unusual and unique properties of its enzymes are of general interest to astrobiologists [5, 9]. Such environments may be analogs of regions of Earth’s sister planet, Mars, where the potential for biological activity is of intense interest. Images from the Mars Reconnaissance Orbiter showed evidence for seasonal emergence of liquid flows in summer, findings consistent with briny liquid water emerging from underground reservoirs on the planet . The Jovian satellite Europa is covered by frozen water-ice and the presence of liquid water beneath the surface has been hypothesized [64, 65]. Further studies of model enzymes like β-galactosidase and the polyextremophilic microbe H. lacusprofundi are likely to provide greater insights into how life may be able to cope with challenging conditions on other worlds.
The genome sequence of H. lacusprofundi and directed expression of the β-galactosidase gene in the model haloarchaeon, Halobacterium sp. NRC-1, provided a polyextremophilic enzyme for investigation. The purified enzyme was found to be active in high salt concentrations (4–4.5 M NaCl and KCl) and both low (<10°C) and high (>50°C) temperatures, as well as in 10–20% aqueous-organic alcohol solutions. The enzyme provides a platform for improving our understanding of biocatalysis under extreme water limiting conditions and for potential applications in synthetic chemistry. H. lacuprofundi encoded proteins and the haloarchaeal genetic system represent significant resources for biotechnology. The system provides the basis for better understanding of life in a perennially cold, hypersaline environment, with relevance to life elsewhere.
This work was funded by the National Aeronautics and Space Administration grant NNX10AP47G. RK was partially supported by an ASM International Fellowship for Asia.
- Capes MD, Coker JA, Gessler R, Grinblat-Huse V, DasSarma SL, Jacob CG, Kim JM, DasSarma P, DasSarma S: The information transfer system of halophilic archaea. Plasmid. 2011, 65: 77-101. 10.1016/j.plasmid.2010.11.005.View ArticleGoogle Scholar
- Capes MD, DasSarma P, DasSarma S: The core and unique proteins of haloarchaea. BMC Genomics. 2012, 13: 39-10.1186/1471-2164-13-39.View ArticleGoogle Scholar
- Anderson I, Scheuner C, Göker M, Mavromatis K, Hooper SD, Porat I, Klenk HP, Ivanova N, Kyrpides N: Novel insights into the diversity of catabolic metabolism from ten haloarchaeal genomes. PLoS One. 2011, 6: e20237-10.1371/journal.pone.0020237.View ArticleGoogle Scholar
- Franzmann PD, Stackebrandt E, Sanderson K, Volkman JK, Cameron DE, Stevenson PL, McMeekin TA, Burton HR: Halobacterium lacusprofundi, sp. nov, a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol. 1988, 11: 20-27. 10.1016/S0723-2020(88)80044-4.View ArticleGoogle Scholar
- Reid IN, Sparks WB, Lubow S, McGrath M, Livio M, Valenti J, Sowers KR, Shukla HD, MacAuley S, Miller T, Suvanasuthi R, Belas R, Colman A, Robb FT, DasSarma P, Müller JA, Coker JA, Cavicchioli R, Chen F, DasSarma S: Terrestrial models for extraterrestrial life: methanogens and halophiles at Martian temperatures. Intl J Astrobiol. 2006, 5: 89-97. 10.1017/S1473550406002916.View ArticleGoogle Scholar
- DasSarma S, DasSarma P: Encyclopedia of life sciences. Halophiles. 2012, London: WileyView ArticleGoogle Scholar
- DasSarma P, Zamora RC, Müller JA, DasSarma S: Genome-wide responses of the model archaeon Halobacterium sp. strain NRC-1 to oxygen limitation. J Bacteriol. 2012, 194: 5530-5537. 10.1128/JB.01153-12.View ArticleGoogle Scholar
- Karan R, Capes MD, DasSarma S: Function and biotechnology of extremophilic enzymes in low water activity. Aquat Biosyst. 2012, 8: 4-10.1186/2046-9063-8-4.View ArticleGoogle Scholar
- DasSarma S: Extreme halophiles are models for astrobiology. Microbe. 2006, 1: 120-127.Google Scholar
- Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B: The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009, 37: 233-238. 10.1093/nar/gkn663.View ArticleGoogle Scholar
- Muller-Hill B: The lac operon: a short history of a genetic paradigm. 1996, Berlin: Walter de GruyterView ArticleGoogle Scholar
- Coker JA, Sheridan PP, Loveland-Curtze J, Gutshall KR, Auman AJ, Brenchley JE: Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an Antarctic Arthrobacter isolate. J Bacteriol. 2003, 185: 5473-5482. 10.1128/JB.185.18.5473-5482.2003.View ArticleGoogle Scholar
- Nakagawa T, Fujimoto Y, Ikehata R, Miyaji T, Tomizuka N: Purification and molecular characterization of cold active beta-galactosidase from Arthrobacter psychrolactophilus strain F2. Appl Microbiol Biotechnol. 2006, 72: 720-725. 10.1007/s00253-006-0339-0.View ArticleGoogle Scholar
- Holmes ML, Scopes RK, Moritz RL, Simpson RJ, Englert C, Pfeifer F, Dyall-Smith ML: Purification and analysis of an extremely halophilic beta-galactosidase from Haloferax alicantei. Biochim Biophys Acta. 1997, 1337: 276-286. 10.1016/S0167-4838(96)00174-4.View ArticleGoogle Scholar
- Ohtsu N, Motoshima H, Goto K, Tsukasaki F, Matsuzawa H: Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing. Biosci Biotechnol Biochem. 1998, 62: 1539-1545. 10.1271/bbb.62.1539.View ArticleGoogle Scholar
- Hu JM, Li H, Cao LX, Wu PC, Zhang CT, Sang SL, Zhang XY, Chen MJ, Lu JQ, Liu YH: Molecular cloning and characterization of the gene encoding cold-active β-galactosidase from a psychrotrophic and halotolerant Planococcus sp. L4. J Agric Food Chem. 2007, 55: 2217-2224. 10.1021/jf062910r.View ArticleGoogle Scholar
- Hidaka M, Fushinobu S, Ohtsu N, Motoshima H, Matsuzawa H, Shoun H, Wakagi T: Trimeric crystal structure of the glycoside hydrolase family 42 beta-galactosidase from Thermus thermophilus A4 and the structure of its complex with galactose. J Mol Biol. 2002, 322: 79-91. 10.1016/S0022-2836(02)00746-5.View ArticleGoogle Scholar
- Sheridan PP, Brenchley JE: Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate. Appl Environ Microbiol. 2000, 66: 2438-2444. 10.1128/AEM.66.6.2438-2444.2000.View ArticleGoogle Scholar
- Hinz SW, Van den Brock LA, Beldman G, Vincken JP, Voragen AG: Beta-galactosidase from Bifidobacterium adolescentis DSM20083 prefers beta(1,4)-galactosides over lactose. Appl Microbiol Biotechnol. 2004, 66: 276-284. 10.1007/s00253-004-1745-9.View ArticleGoogle Scholar
- Shipkowski S, Brenchley JE: Characterization of an unusual cold-active beta-glucosidase belonging to family 3 of the glycoside hydrolases from the psychrophilic isolate Paenibacillus sp. strain C7. Appl Environ Microbiol. 2005, 7: 4225-4232.View ArticleGoogle Scholar
- Shipkowski S, Brenchley JE: Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases. Appl Environ Microbiol. 2006, 72: 7730-7738. 10.1128/AEM.01306-06.View ArticleGoogle Scholar
- Gupta A, Khare SK: Enzymes from solvent-tolerant microbes: useful biocatalysts for non-aqueous enzymology. Crit Rev Biotechnol. 2009, 29: 44-54. 10.1080/07388550802688797.View ArticleGoogle Scholar
- Doukyua N, Ogino H: Organic solvent-tolerant enzymes. Biochem Eng J. 2010, 48: 270-282. 10.1016/j.bej.2009.09.009.View ArticleGoogle Scholar
- DasSarma S, Robb FT, Place AR, Sowers KR, Schreier HJ, Fleischmann EM: Archaea: a laboratory manual – halophiles. 1995, New York: Cold Spring Harbor Laboratory PressGoogle Scholar
- Berquist BR, Müller JA, DasSarma S: Methods in microbiology. Genetic systems for halophilic archaea. Edited by: Oren A, Rainey F. 2006, London: Elsevier/Academic Press, 649-680. Volume 35thGoogle Scholar
- Hildebrandt P, Wanarska M, Kur J: A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties. BMC Microbiol. 2009, 9: 1-11. 10.1186/1471-2180-9-1.View ArticleGoogle Scholar
- Miller JH: Experiments in molecular genetics. 1972, New York: Cold Spring Harbor Laboratory PressGoogle Scholar
- DasSarma SL, Capes MD, DasSarma P, DasSarma S: HaloWeb: the haloarchaeal genomes database. Saline Systems. 2010, 6: 12-10.1186/1746-1448-6-12.View ArticleGoogle Scholar
- Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA: The COG database: an updated version includes eukaryotes. BMC Bioinformatics. 2003, 4: 41-10.1186/1471-2105-4-41.View ArticleGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.View ArticleGoogle Scholar
- Altschul SF, Wootton JC, Gertz EM, Agarwala R, Morgulis A, Schäffer AA, Yu YK: Protein database searches using compositionally adjusted substitution matrices. FEBS J. 2005, 272: 5101-5109. 10.1111/j.1742-4658.2005.04945.x.View ArticleGoogle Scholar
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.View ArticleGoogle Scholar
- Coker JA, DasSarma P, Kumar J, Müller JA, DasSarma S: Transcriptional profiling of the model Archaeon Halobacterium sp. NRC-1: responses to changes in salinity and temperature. Saline Systems. 2007, 3: 6-10.1186/1746-1448-3-6.View ArticleGoogle Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3.View ArticleGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0.View ArticleGoogle Scholar
- Keller A, Nesvizhskii AI, Kolker E, Aebersold R: Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem. 2002, 74: 5383-5392. 10.1021/ac025747h.View ArticleGoogle Scholar
- Kennedy SP, Ng WV, Salzberg SL, Hood L, DasSarma S: Understanding the adaptation of Halobacterium species NRC-1 to its extreme environment through computational analysis of its genome sequence. Genome Res. 2001, 11: 1641-1650. 10.1101/gr.190201.View ArticleGoogle Scholar
- Ng WV, Kennedy SP, Mahairas GG, Berquist B, Pan M, Shukla HD, Lasky SR, Baliga NS, Thorsson V, Sbrogna J, Swartzell S, Weir D, Hall J, Dahl TA, Welti R, Goo YA, Leithauser B, Keller K, Cruz R, Danson MJ, Hough DW, Maddocks DG, Jablonski PE, Krebs MP, Angevine CM, Dale H, Isenbarger TA, Peck RF, Pohlschroder M, Spudich JL, Jung KW, Alam M, Freitas T, Hou S, Daniels CJ, Dennis PP, Omer AD, Ebhardt H, Lowe TM, Liang P, Riley M, Hood L, DasSarma S: Genome sequence of Halobacterium species NRC-1. Proc Natl Acad Sci USA. 2000, 97: 12176-12181. 10.1073/pnas.190337797.View ArticleGoogle Scholar
- Hackett NR, DasSarma S: Characterization of the small endogenous plasmid of Halobacterium strain SB3 and its use in transformation of H. halobium. Can J Microbiol. 1989, 35: 86-91. 10.1139/m89-013.View ArticleGoogle Scholar
- Jung KH, Spudich JL: Suppressor mutation analysis of the sensory rhodopsin I-transducer complex: insights into the color-sensing mechanism. J Bacteriol. 1998, 180: 2033-2042.Google Scholar
- Halladay JT, Jones JG, Lin F, MacDonald AB, DasSarma S: The rightward gas vesicle operon in Halobacterium plasmid pNRC100: identification of the gvpA and gvpC gene products by use of antibody probes and genetic analysis of the region downstream of gvpC. J Bacteriol. 1993, 175: 684-692.Google Scholar
- DasSarma P, Coker JA, Huse V, DasSarma S: Halophiles, biotechnology. Encyclopedia of industrial biotechnology, bioprocess, bioseparation, and cell technology. Edited by: Flickinger MC. 2010, New York: John Wiley and Sons, 2769-2777.Google Scholar
- Hoyoux A, Jennes I, Dubois P, Genicot S, Dubail F, François JM, Baise E, Feller G, Gerday C: Cold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis. Appl Environ Microbiol. 2001, 67: 1529-1535. 10.1128/AEM.67.4.1529-1535.2001.View ArticleGoogle Scholar
- Oliveira C, Guimarães PM, Domingues L: Recombinant microbial systems for improved β-galactosidase production and biotechnological applications. Biotechnol Adv. 2011, 29: 600-609.View ArticleGoogle Scholar
- Cavicchioli R, Charlton T, Ertan H, Omar SM, Siddiqui KS, Williams TJ: Biotechnological uses of enzymes from psychrophiles. Microbial Biotechnol. 2011, 4: 449-460. 10.1111/j.1751-7915.2011.00258.x.View ArticleGoogle Scholar
- Struvay C, Feller G: Optimization to low temperature activity in psychrophilic enzymes. Int J Mol Sci. 2012, 13: 11643-11665. 10.3390/ijms130911643.View ArticleGoogle Scholar
- Madern D, Ebel C, Zaccai G: Halophilic adaptation of enzymes. Extremophiles. 2000, 4: 91-98. 10.1007/s007920050142.View ArticleGoogle Scholar
- Queiroz JA, Tomaz CT, Cabral JM: Hydrophobic interaction chromatography of proteins. J Biotechnol. 2001, 87: 143-159. 10.1016/S0168-1656(01)00237-1.View ArticleGoogle Scholar
- Karan R, Khare SK: Purification and characterization of a solvent stable protease from Geomicrobium sp. EMB2. Environ Technol. 2010, 10: 1061-1072.View ArticleGoogle Scholar
- Schmitt W, Rdest U, Goebel W: Efficient high performance liquid chromatographic system for the purification of a halobacterial serine protease. J Chromatogr. 1990, 521: 211-220. 10.1016/0021-9673(90)85045-W.View ArticleGoogle Scholar
- García-Ortega L, De los Ríos V, Martínez-Ruiz A, Oñaderra M, Lacadena J, Martínez del Pozo A, Gavilanes JG: Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2. Electrophoresis. 2005, 26: 3407-3413. 10.1002/elps.200500261.View ArticleGoogle Scholar
- Oren A: Life at high salt concentrations. The prokaryotes. A handbook on the biology of bacteria: ecophysiology and biochemistry volume 2. Edited by: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. 2006, New York: Springer, 263-282.Google Scholar
- Wang K, Li G, Yu SQ, Zhang CT, Liu YH: A novel metagenome-derived beta-galactosidase: gene cloning, overexpression, purification and characterization. Appl Microbiol Biotechnol. 2010, 88: 155-165. 10.1007/s00253-010-2744-7.View ArticleGoogle Scholar
- Karasova-Lipovova P, Strnad H, Spiwok V, Mala S, Kralova B, Russell N: The cloning, purification and characterization of a cold-active β-galactosidase from the psychrotolerant antarctic bacterium Arthrobacter sp. C2–2. Enz Microb, Technol. 2003, 33: 836-844. 10.1016/S0141-0229(03)00211-4.View ArticleGoogle Scholar
- Xu K, Tang X, Gai Y, Mehmood M, Xiao X, Wang F: Molecular characterization of cold-inducible beta-galactosidase from Arthrobacter sp. ON14 isolated from Antarctica. J Microbiol Biotechnol. 2011, 21: 236-242.Google Scholar
- Wierzbicka-Woś A, Cieśliński H, Wanarska M, Kozłowska-Tylingo K, Hildebrandt P, Kur J: A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d-gene cloning, purification and characterization. Microb Cell Fact. 2011, 10: 108-10.1186/1475-2859-10-108.View ArticleGoogle Scholar
- Karan R, Singh SP, Kapoor S, Khare SK: A novel organic solvent tolerant protease from a newly isolated Geomicrobium sp. EMB2 (MTCC 10310): production optimization by response surface methodology. N Biotechnol. 2011, 28: 136-145. 10.1016/j.nbt.2010.10.007.View ArticleGoogle Scholar
- Ruiz DM, De Castro RE: Effect of organic solvents on the activity and stability of an extracellular protease secreted by the haloalkaliphilic archaeon Natrialba magadii. J Ind Microbiol Biotechnol. 2007, 34: 111-115. 10.1007/s10295-006-0174-4.View ArticleGoogle Scholar
- Fukushima T, Mizuki T, Echigo A, Inoue A, Usami R: Organic solvent tolerance of halophilic α-amylase from a Haloarchaeon, Haloarcula sp. strain S-1. Extremophiles. 2005, 9: 85-89. 10.1007/s00792-004-0423-2.View ArticleGoogle Scholar
- Maugard T, Gaunt D, Legoy MD, Besson T: Microwave-assisted synthesis of galacto-oligosaccharides from lactose with immobilized beta-galactosidase from Kluyveromyces lactis. Biotechnol Lett. 2003, 25: 623-629. 10.1023/A:1023060030558.View ArticleGoogle Scholar
- Bridiau N, Issaoui N, Maugard T: The effects of organic solvents on the efficiency and regioselectivity of N-acetyl-lactosamine synthesis, using the β-galactosidase from Bacillus circulans in hydro-organic media. Biotechnol Prog. 2010, 26: 1278-1289. 10.1002/btpr.445.View ArticleGoogle Scholar
- Vic G, Thomas D, Crout DHG: Solvent effect on enzyme-catalyzed synthesis of β-D-glucosides using the reverse hydrolysis method: Application to the preparative-scale synthesis of 2-hydroxybenzyl and octyl β-D-glucopyranosides. Enzym Microb Tech. 1997, 20: 597-603. 10.1016/S0141-0229(96)00201-3.View ArticleGoogle Scholar
- McEwen AS, Ojha L, Dundas CM, Mattson SS, Byrne S, Wray JJ, Cull SC, Murchie SL, Thomas N, Gulick VC: Seasonal flows on warm Martian slopes. Science. 2011, 333: 740-743. 10.1126/science.1204816.View ArticleGoogle Scholar
- Khurana KK, Kivelson MG, Russell CT: Searching for liquid water in Europa by using surface observatories. Astrobiol. 2002, 2: 93-103. 10.1089/153110702753621376.View ArticleGoogle Scholar
- Marion GM, Fritsen CH, Eicken H, Payne MC: The search for life on Europa: limiting environmental factors, potential habitats, and Earth analogues. Astrobiol. 2003, 3: 785-811. 10.1089/153110703322736105.View ArticleGoogle Scholar
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