Table 1 Oligonucleotides used in this study as primers for PCR
Primer | 5′-3′ sequence | Use |
|---|---|---|
cspD2F | CGGGGTACCGGCACTGCACAGCCGATGCT | Amplification of cspD2 promoter |
cspD2R | GGAATTCCATATGCAATCAACCGTTGGGCCG | |
HlaR | CGGGATCCCTACGTCTGAGCGATTCT | Amplification of bga gene |
HlaF | GGGAATTCCATATGCGTCTCGGAGTCTG | |
β-gal 05R | TCGAAGGAGCCGTACTGCTG | Sequencing of bga gene |
β-gal 15 F | CCGTCGATCAGCACGAGTCG | |
β-gal 04 F | GATTGTGCCGACGCGTTCCG | |
β-gal 16R | TCCAGCCACTCCGCCCAGGT | |
β-gal 11R | TCCTCGGACGTGTGTGCGGC | |
β-gal l10F | GCACCACGCCGCCGCCCACG | |
pKJprmhtrF | TTATTCCGTTTCCGCGGAAA | Sequencing of cspD2 promoter in pMC2 plasmid |
pKJprmhtrR | GTCGTCCTCGTTGTCCCCGA |