Figure 2From: Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundiShuttle vector pMC2 used for the expression of H. lacusprofundi bga in Halobacterium sp. NRC-1. Location and transcriptional orientation of bla, β-lactamase for ampicillin resistance; mev, HMG-CoA reductase for mevinolin resistance; bga, β-galactosidase, and rep, the Halobacterium pGRB replicase gene are shown. Position of the temperature induced promoter, Pcs→ and KpnI, NdeI and BamHI restriction sites are indicated.Back to article page