Table 2 Purification of H. lacusprofundi β-galactosidase
Purification step | Total protein (mg) | Total activity (IU) | Yield (%) | Specific activity (units/mg protein) | Fold purification |
|---|---|---|---|---|---|
Cell lysate | 7.92 | 29.84 | 100.00 | 3.76 | 1.00 |
Gel filtration | 1.31 | 23.99 | 80.42 | 18.31 | 4.87 |
Hydrophobic interaction chromatography | 0.048 | 5.32 | 17.82 | 110.83 | 29.47 |