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Table 2 Purification of H. lacusprofundi β-galactosidase

From: Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

Purification step Total protein (mg) Total activity (IU) Yield (%) Specific activity (units/mg protein) Fold purification
Cell lysate 7.92 29.84 100.00 3.76 1.00
Gel filtration 1.31 23.99 80.42 18.31 4.87
Hydrophobic interaction chromatography 0.048 5.32 17.82 110.83 29.47