- Research article
- Open Access
Recombinant production and characterization of full-length and truncated β-1,3-glucanase PglA from Paenibacillussp. S09
BMC Biotechnology volume 13, Article number: 105 (2013)
β-1,3-Glucanases catalyze the hydrolysis of glucan polymers containing β-1,3-linkages. These enzymes are of great biotechnological, agricultural and industrial interest. The applications of β-1,3-glucanases is well established in fungal disease biocontrol, yeast extract production and wine extract clarification. Thus, the identification and characterization of novel β-1,3-glucanases with high catalytic efficiency and stability is of particular interest.
A β-1,3-glucanase gene designated PglA was cloned from a newly isolated strain Paenibacillus sp. S09. The gene PglA contained a 2631-bp open reading frame encoding a polypeptide of 876 amino acids which shows 76% identity with the β-1,3-glucanase (BglH) from Bacillus circulans IAM1165. The encoded protein PglA is composed of a signal peptide, an N-terminal leader region, a glycoside hydrolase family 16 (GH16) catalytic domain and a C-terminal immunoglobulin like (Ig-like) domain. The Escherichia coli expression system of PglA and five truncated derivatives containing one or two modules was constructed to investigate the role of catalytic and non-catalytic modules. The pH for optimal activity of the enzymes was slightly affected (pH 5.5-6.5) by the presence of different modules. However, the temperature for optimal activity was strongly influenced by the C-terminal domain and ranged from 50 to 60°C. Deletion of C-terminal domain resulted in obviously enhancing enzymatic thermostability. Specific activity assay indicated that PglA specifically hydrolyzes β-1,3-glucan. Insoluble β-1,3-glucan binding and hydrolysis were boosted by the presence of N-and C-terminal domains. Kinetic analysis showed that the presence of N-and C-terminus enhances the substrate affinity and catalytic efficiency of the catalytic domain toward laminarin. Carbohydrate-binding assay directly confirmed the binding capabilities of the N-and C-terminal domains.
This study provides new insight into the impacts of non-catalytic modules on enzymatic properties of β-1,3-glucanase. Activity comparison of full-length PglA and truncated forms revealed the negative effect of C-terminal region on thermal stability of the enzyme. Both the N-and C-terminal domains exerted strong binding activity toward insoluble β-1,3-glucan, and could be classified into CBM families.
β-1,3-Glucanase catalyzes the hydrolysis of β-1,3-glucosidic bonds existing in β-1,3-glucan, which is the main cell wall component of yeast and filamentous fungi, and a major structural and storage polysaccharides (laminarin) in marine macroalgae, and produced as exopolysaccharide by some bacteria . β-1,3-Glucanases are widely distributed among from plants, marine animals, fungi and bacteria. According to the divergence of amino acid sequence, β-1,3-glucanases from plants are divided into glycosyl hydrolase (GH) family 17 while the bacterial enzymes are grouped into GH16 family . β-1,3-Glucanases synthesized in plants are highlighted because of the plant defense against fungal pathogens. Additionally, they also play roles in the normal biological and developmental processes in plants and fungi. In bacteria, β-1,3-glucanases participate in the degradation of polysaccharides which are utilized as an nutrient source [3, 4].
There has been a great deal of interest in research of bacterial β-1,3-glucanases. They have shown potential biotechnical applications in structural analysis of yeast and fungal cell wall , and preparation of protoplasts . β-1,3-Glucanase could also act as a potential biocontrol agent against pathogenic fungi in agriculture , and a useful tool to prevent slime production and undesirable yeast growth during vinification in the wine-making process . In relation to pharmaceutical application, the soluble β-1,3-glucan oligosaccharides digested by β-1,3-glucanase are potential immuneactivators . Based on the substrates hydrolyzing mechanisms, β-1,3-glucanases are classified into two types: exo-1,3-β-D-glucanases (EC 126.96.36.199) which release glucose residues from the non-reducing end of a substrate, and endo-1,3-β-D-glucanases (EC 188.8.131.52 and EC 184.108.40.206) which catalyze the hydrolysis of internal β-1,3-glucosidic bonds at random sites and release short oligosaccharides . There are many β-1,3-glucanases that have been purified and characterized from different bacterial species, including Bacillus, Paenibacillus[12, 13], Micromonospora, Streptomyces and Cellulosimicrobium. Proposed physiological functions of bacterial β-1,3-glucanases vary depending on the source and structure of enzyme . In view of the industrial and biotechnological importance of β-1,3-glucanase, there is of great potential interest in identifying and characterizing novel β-1,3-glucanases.
In the present study, a novel β-1,3-glucanase gene was identified by from Paenibacillus sp. S09, which was isolated from the rhizosphere of oat plants. The deduced amino acid sequence analysis and homology search revealed that this protein contained an N-terminal leader region, a GH16 catalytic domain and a C-terminal domain with Ig-like fold. The important role of Ig-like domain has been revealed in many cellulases, but little is known about the biochemical properties of Ig-like domain in bacterial β-1,3-glucanases. To investigate the role of the N- and C-terminal domains in enzymatic function, the Escherichia coli (E. coli) expression system of PglA and its truncated mutants was constructed in this study. Here, we report the cloning of a new β-1,3-glucanase gene pglA from S09, the construction and purification of the truncated enzymes, the comparison of the enzymes in terms of their biochemical characteristics and affinities to insoluble β-1,3-glucans. To the best of our knowledge, this is the first report that describes function activity relationship between catalytic and non-catalytic domains of a bacterial β-1,3-glucanase which containing a C-terminal Ig-like fold.
Strains, media, vectors and chemicals
The strain Paenibacillus sp. S09 (CCTCC accession no:M2012196; China Center for Type Culture Collection, Wuhan, China) was originally isolated from the rhizosphere of oat plants. Luria-Berntani (LB) broth supplemented with 0.5% pachyman was used for the production of β-1,3-glucanase by strain S09. E. coli competent cell DH5α (Takara, Otsu, Japan) and the plasmid pCR2.1 vector (Invitrogen, Carlsbad, CA, USA) were used for gene cloning. E. coli BL21 (DE3) and the pET-29a(+) vector (Novagen, San Diego, CA, USA) were used for gene expression. Recombinant E. coli was cultured in LB broth supplemented with kanamycin (50 μg/ml) at 37°C. Recombinant E. coli BL21 (DE3) harboring the expression vector was cultivated in 2 × YT medium which containing 1.0% (w/v) yeast extract, 1.6% (w/v) peptone and 0.5% (w/v) NaCl. The His6-tagged protein was purified by Ni-sepharose 6 fast flow column (GE healthcare, Sweden). The DNA purification kit, restriction endonucleases, T4 DNA ligase, pfu and LA Taq DNA polymerase with GC buffer and dNTPs were purchased from Takara. Isopropyl-β-D-thiogalactopyranoside (IPTG) and 5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-Gal) were also purchased from Takara.
Laminarin (from Laminarium digitatum), barley β-glucan, zymosan A were purchased from Sigma-Aldrich (Co., St. Louis, USA). Pachyman was purified from commercial fruiting bodies of the basidiomycete Poria cocos. Carboxymethyl cellulose-sodium (CMC) and dextran were also purchased from Sigma. Salecan, a novel soluble linear β-1,3-glucan with α-1,3-linkages, were prepared from Agrobacterium sp. ZX09, as described by Xiu et al. . All other reagents were of analytical grade.
Molecular cloning of the β-1,3-glucanase encoding gene
The genomic DNA of strain S09 was extracted as described by Sambrook and Russell . In order to obtain partial of the β-1,3-glucanase gene sequence, degenerate primers (Pdg-F and Pdg-R) was designed based on the two conserved amino acid sequences of GH16 β-1,3-glucanases (Table 1, see Additional file 1: Figure S1). the PCR was performed to amplify a partial β-1,3-glucanase gene as follows: 94°C for 5 min; 30 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 1 min; one final extension at 72°C for 10 min. The genomic DNA of strain S09 was used as the template. The products of PCR reaction were gel-purified, ligated into pCR2.1 vector, transformed into DH5α and sequenced by Invitrogen. Inverse PCR (I-PCR) and Self-Formed Adaptor PCR (SEFA-PCR) were employed to obtain the full sequence of β-1,3-glucanase gene according to the manufacturer’s instructions . The primers used in I-PCR and SEFA-PCR were listed in Table 1. The primers “gIF1, gIF2, gIR1, gIR2” constructed according the partial β-1,3-glucanase gene were used in I-PCR. Briefly, genomic DNA was digested with HindШ for 3 h. The digested genomic DNA fragments were gel-purified and subsequently circularized by use of the DNA ligation kit (Takara). I-PCR amplification was conducted with the circulated DNA as template. The primers 5′-sp1, 5′-sp2, and 5′-hemi-sp3 were used to amplify the 5′ flanking sequence, 3′-sp1, 3′-sp2, and 3′-hemi-sp3 were used to amplify the 3′ flanking sequence of the partial β-1,3-glucanase gene. The detailed procedures for SEFA-PCR were shown in Additional file 2. The amplified PCR products were purified, cloned into the pCR2.1 plasmid, and sequenced.
Nucleotide sequences were assembled using the DNAMAN software package (Version 5.2.2, Lynnon BioSoft, USA). The signal peptide was predicted using SignalP 4.0 server (http://www.cbs.dtu.dk/services/SignalP) . The alignments of DNA and protein sequences were performed at National Center for Biotechnology Information (NCBI) using the blastn and blastp program (http://www.ncbi.nlm.nih.gov/BLAST/), respectively. The conserved domains and classification of GH family were identified with the website http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml. Protein secondary structure was predicted using PSIPRED 3.0  (http://bioinf.cs.ucl.ac.uk/psipred). Structures of endo-1,3-β-glucanase from Cellulosimicrobium cellulans (PDB ID: 3atgA) and the C-terminal Ig-like domain of the bacteriophage λ tail tube protein (PDB ID: 2l04A) were used as templates for protein structure homology modeling using the automated mode of SWISS-MODEL . Multiple sequence alignments were performed with ClustalX2 . Distance matrix for nucleotides was calculated according to the Kimura’s two-parameter model . Phylogenetic tree, assessed by 1,000 bootstraps, was constructed using neighbor-joining algorithms with MEGA 5.0 software .
Construction of expression vectors
Five truncated mutants containing one or two modules were designed according to the conserved domain analysis (Figure 1). The corresponding coding regions were amplified using respective pairs of primers by PCR (Table 2). The amplification reactions performed with pfu DNA polymerase consisted of 30 cycles of 94°C for 30 s, 60°C for 30 s, 72°C for 1-3 min, followed by a 10 min extension at 72°C. The PCR-amplified fragments were purified and digested with KpnІ and HindШ and then ligated into the expression vector pET-29a(+) with a C-terminal His6-tag. After transformation into E. coli DH5α, positive transformants were screened and checked by DNA sequencing. The recombinant plasmids were used to transform E. coli BL21 (DE3) competent cells for protein expression.
Gene expression and protein purification
For recombinant protein production, recombinant E.coli BL21 cells harboring the expression vectors were first inoculated into LB medium supplemented with kanamycin (50 μg/ml) and grown overnight at 37°C. Then, the culture was transferred into fresh 2 × YT medium (5:100 dilution) containing 50 μg/ml kanamycin and grown at 37°C to an OD600 ≈ 0.8, induced with 0.1 mM IPTG and further cultivated for 6-8 h at 25°C. The culture medium was centrifuged at 12,000 × g for 10 min at 4°C. Cells were washed and suspended in PBS buffer, lysed by sonication on ice and centrifuged at 15,000 × g for 20 min at 4°C to remove the cell debris. The supernatant was applied to Ni-sepharose 6 fast flow column (GE healthcare, Sweden) that had been equilibrated with binding buffer (20 mM sodium phosphate, 500 mM NaCl, pH 7.2). The protein was eluted using a linear gradient of elution buffer (20 mM sodium phosphate, 500 mM NaCl, 15-300 mM imidazole, pH 7.2). Fractions having the β-1,3-glucanase activity were pooled and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Enzyme activity assay and protein determination
The β-1,3-glucanase activity assay was carried out by measuring the amount of reducing sugars released from a β-1,3-glucan substrate (laminarin) according to the 3,5-dinitrosalicylic acid (DNS) method . Unless otherwise stated, the reaction mixture was incubated at 55°C for 20 min using laminarin (5 mg/ml) as the substrate in 20 mM sodium acetate buffer (pH 6.0). The amount of reducing sugar was then determined spectrophotometrically at 550 nm. One unit (U) of enzyme activity was defined as the amount of enzyme required to release reducing sugars that equivalent to 1 μmol glucose per min under the test conditions. Hydrolytic activities toward insoluble substrates were performed by gently mixing reaction mixtures during incubation. Then the mixture was centrifuged at 12,000 × g at 4°C for 10 min. The reducing sugars in the supernatant were measured by the DNS method. The protein concentration was measured by the bicinchoninic acid (BCA) method using the BCA protein assay reagent (Thermo Fisher Scientific, MA, USA), Bovine serum albumin (BSA) was used as the standard protein.
With laminarin as the substrate, the optimal temperature for β-1,3-glucanase activity was determined over the range of 30-80°C in sodium acetate buffer (pH 6.0). Enzyme thermostability was determined by measuring the residual activity after pre-incubation of the enzyme in sodium acetate buffer (pH 6.0) at 55°C without substrate for up to 60 min. The optimal pH for β-1,3-glucanase activity was determined at 55°C in buffers with pH ranging from 2.0 to 10.0. The enzyme stability at different pH was estimated by measuring the residual enzyme activity after incubating the enzyme solution in buffers with pH of 2.0-12.0 at 4°C for 4 h. The buffers (50 mM) used were glycine-HCl (pH 2.0-4.0), sodium acetate (pH 5.0-7.0), Tris–HCl (pH 7.5-8.5) and glycine-NaOH (pH 9.0-12.0).
The substrate specificity was assayed in reactions with 1% laminarin, pachyman, zymosan A, barley β-glucan, CMC, dextran and 0.5% salecan under the optimal conditions for each enzyme. The reaction mixture was often oscillated slightly during incubation for insoluble polysaccharides.
Kinetic study was performed with laminarin as the substrate under the optimal conditions for each enzyme. The initial velocities under laminarin concentrations (0.5-5 mg/ml) were measured, and the values of Km and Vmax were obtained from Lineweaver-Burk plots.
Binding strength of the enzymes to insoluble polysaccharides was estimated by mixing 15-20 μg of the purified proteins with various substrates (10 mg) in sodium acetate buffer (200 μl) at 4°C for 1 h with gentle shaking. Binding mixture without substrate was set as control. The binding ability of enzymes containing catalytic domain to insoluble polysaccharides was determined by measuring the residual enzyme activity in the supernatant of the binding mixture. The amount of the enzyme bound to the polysaccharide was estimated by subtraction of the activity recovered from the original activity . The binding ability of non-catalytic domain to insoluble substrates was also determined . After 1 h of binding reaction, the fraction of protein in the supernatant and the pellet was estimated by 12% SDS-PAGE analysis. BSA was used as the negative control and was handled similarly to the other samples.
The nucleotide sequence for the GH16 β-1,3-glucanase gene pglA were deposited in GenBank database under the accession number JX070086.
Cloning of the β-1,3-glucanase gene and sequence analysis
A β-1,3-glucanase-encoding gene designated PglA was identified from a 5053-bp fragment which was obtained by using degenerate PCR, I-PCR and SEFA-PCR. The deduced amino acid sequence of PglA (GenBank No.: AFO67889.1) comprises 876 amino acids with a predicted N-terminal signal peptide (residues 1-38) (Figure 2). The mature protein consists of 838 amino acid residues with a calculated pI of 4.5 and molecular mass of 90.4 kDa, respectively. The deduced amino acid sequence of PglA exhibited the highest identity (86%) with the hypothetical protein from Paenibacillus daejeonensis (NCBI Reference Sequence: WP_020617344.1), 77% identity with the glycoside hydrolase family 16 protein from Paenibacillus lactis 154 (GenBank No.: EHB65983.1), and 76% identity with the reported β-1,3-glucanase BglH from Bacillus circulans IAM1165 (GenBank No.: AAC60453.1). PglA showed only 45% identity with the well-characterized endo-β-1,3-glucanase LamA from Paenibacillus sp. CCRC 17245 (GenBank No.: ABJ15796.1). Combing the results of conserved domain search in the Conserved Domain Database (CDD) of NCBI, secondary structure prediction and tertiary structure homology modeling by SWISS-MODEL (See Additional file 3: Figure S2), PglA contains a GH16 laminarinase-like domain and a bacterial Ig-like domain (Figures 2 and 3A). The domain organization of PglA is not similar to LamA, which include a leader peptide, a threefold repeat of S-layer homologous module, a GH16 catalytic module, four repeats of CBM_4_9 and an analogue of coagulation factor Fa5/8C from N to C terminus . PglA could be considered as a novel β-1,3-glucanase of Paenibacillus species. Phylogenetic tree showed that the catalytic region of PglA forms a distinct clade with BglH from B. circulans and putative glycoside hydrolases from Paenibacillus species (Figure 3B). Phylogenetic analysis based on the Ig-like domain sequence alignment indicated the relationships between PglA and other Ig-like domain containing proteins (Figure 3C).
Expression of full-length and truncated PglA in E.coliand protein purification
Using basic molecular cloning techniques, the gene fragment of PglA without the signal peptide sequence and five truncated mutants were cloned into pET-29a(+) vector and successfully expressed in E.coli BL21 (DE3). Figure 1A represents a schematic overview of the recombinantly produced constructs. The His6-tagged enzymes were purified by affinity chromatography on a Ni-sepharose 6 fast flow column. Molecular masses of rPglA and its truncated mutants rPglA∆C, rPglA∆N, rPglA-CD, rPglA-N and rPglA-C were estimated to be 91 kDa, 70 kDa, 49 kDa, 29 kDa, 42 kDa and 21 kDa, respectively. SDS-PAGE analysis indicated protein bands with molecular masses corresponding well to the theoretical values. All the enzymes were purified to a homogeneous state on SDS-PAGE (Figure 1B). The steps involved in a typical purification process are summarized in Table 3.
Effects of temperature and pH on the enzymatic activity and stability
The general properties including the optimal pH and temperature of PglA and the truncated derivatives were investigated with laminarin as the substrate. Deletion of the N-or C-terminal domain did not abolish the hydrolytic function of β-1,3-glucanase, thus, enzymatic properties of rPglA, rPglA∆C, rPglA∆N and rPglA-CD were characterized respectively.
Effect of domains on thermoactivity and thermostability of rPglA was investigated. Side by side comparison of the temperature activity profiles of rPglA and the truncated mutants (rPglA∆C, rPglA∆N and rPglA-CD) showed that, rPglA∆N, the PglA variant containing the catalytic domain and the C-terminal Ig-like domain, obviously is the least thermoactive enzyme. It had its highest activity at 50°C, while, rPglA, rPglA∆C and rPglA-CD have a maximum activity at 60°C (Figure 4A). This phenomenon indicated that the deletion of the C-terminus increased the optimal temperature. As a dramatic loss of enzymatic stability occurred in the full length enzyme (rPglA) at temperatures higher than 60°C, the thermal stability was assayed at 55°C for up to 60 min. In conjunction with the temperature optima, also the thermal stability of the truncated derivatives of PglA turned out to be module related. As observed by the thermal stability assay, the absence of C-terminal region is of great relevance for higher temperature stability of the proteins. This can be deduced from the unexpected higher stability of rPglA-CD and rPglA∆C, and lower stability of rPglA and rPglA∆N (Figure 4B). These results indicated that truncation of C-terminal domain improved the thermostability of PglA.
Deletion of the N-or C-terminal regions had no drastic effect on the optimal pH. For all PglA derived constructs, an apparent pH optimum between pH 5.5 and 6.5 was observed (Figure 4C). rPglA and the truncated enzymes showed broad pH stability. All enzymes retained more than 80% of their original enzymatic activity after 4 h incubation in buffers with pH values between pH 5.0 and 10.0 (Figure 4D). It should be noted that the C-terminal domain deleted mutant rPglA∆C retained higher activity in the acidic range of the pH spectrum (pH 2.0-4.5) than the other deletion derivatives.
Substrate specificity and kinetic analysis
The purified rPglA and truncated enzymes (PglA∆C, PglA∆N and PglA-CD) shared similar substrate specificity. They were highly active toward soluble β-1,3-glucan (laminarin) and insoluble β-1,3-glucan (pachyman and zymosan A). Low activity was detected on soluble linear glucan, barley β-glucan which contains mixed β-1,3-and β-1,4- linkages, and salecan which contains mixed β-1,3-and α-1,3-linkages, little activity on CMC, dextran and xylan (Table 4). It was not surprising as β-1,3-glucan specificity is the general rule for laminarinases. Side by side comparison of the specific activity of rPglA and the truncated mutants (rPglA∆C, rPglA∆N and rPglA-CD) showed that, both the N-and C-terminal domains could enhance the hydrolyzing activity of the catalytic module toward insoluble substrates. It was worth noting that rPglA∆C and rPglA-CD show slightly higher activity toward laminarin than rPglA and rPglA∆N. It should be related to the improved thermostability of rPglA∆C and rPglA-CD as the deletion of the C-terminal region.
The kinetic parameters with laminarin as substrate were determined from substrate concentration dependence of velocity. Based on double reciprocal plots, the values for the Michaelis constant (Km), turnover number (kcat) and catalytic efficiency (kcat/Km) are calculated and presented in Table 5. Km values of rPglA, rPglA∆C, rPglA∆N and rPglA-CD were calculated to be 0.94, 1.01, 1.36 and 2.64 mg/ml, respectively, suggesting that deleting N-and C-terminal domains caused significantly increased Km values. The kcat/Km values of rPglA∆C, rPglA∆N and rPglA-CD were about 70%, 78% and 35% of that of rPglA respectively. These kinetic parameters indicate that the N-and C-terminal domains increase the affinity of PglA for the substrate (laminarin) and thus enhance the catalytic efficiency of catalytic module.
Binding experiments with rPglA and the truncated derivatives and BSA as the tested protein were performed. Two approaches were taken to explore the polysaccharide-binding function of the N-terminal and C-terminal regions. First, as for rPglA and the truncated enzymes containing the catalytic domain (rPglA∆C, rPglA∆N, rPglA-CD), residual enzymatic activity in the supernatant of binding mixtures was measured. As shown in Figure 5A, the catalytic domain of PglA (rPglA-CD) had the minimum binding ability compared to the other three enzymes. Both the N-and C-terminal regions of PglA had significantly carbohydrate-binding activities. These results were confirmed by the insoluble carbohydrate-binding analysis of rPglA-N and rPglA-C. The N-and C-terminal domain bound strongly to insoluble β-1,3-glucan (pachyman and zymosan A), as a large fraction of the protein was retained in the pellet fraction, but not to xylan (Figure 5B). Presence of insoluble polysaccharide did not affect the amount of BSA in supernatant, excluding the nonspecific binding of insoluble polysaccharide to protein.
Enzymes that hydrolyze insoluble complex polysaccharide structures are usually multi-modular enzymes and composed of distinct catalytic domain and non-catalytic carbohydrate binding modules (CBMs) that play a pivotal role in the action of these enzymes against recalcitrant substrates . The presently known CBMs are classified into 67 families in the latest update of the CAZy database (http://www.cazy.org/fam/acc_CBM.html). Among the 67 CBMs families classified in CAZy, one or more of CBMs of 4, 6, 9, 13, 54, 56 families have been found in bacterial β-1,3-glucanases [13, 27, 29–31]. In the work reported here, a novel β-1,3-glucanase gene PglA was cloned from Paenibacillus sp.S09. PglA contains three functional regions: an N-terminal domain, a GH16 catalytic domain, and a C-terminal domain with an Ig-like structure. Even high sequence similarity was found between PglA and the β-1,3-glucanase BglH from Bacillus circulans IAM1165, PglA possessed a novel Ig-like domain which was not observed in BglH by conserved domain search in NCBI. Previous reports demonstrated carbohydrate-binding properties of the N-and C-terminal regions of BglH [27, 32], while, the effects of the non-catalytic domain on enzymatic properties of BglH were not described. Furthermore, the enzymatic properties of Ig-like domain, which is generally considered being involved in carbohydrate binding and commonly found in bacterial proteins , have not been illustrated in bacterial β-1,3-glucanases. We were interested to investigate the functional structural elements of PglA.
It is generally believed that each functional module in a polypeptide can function normally even after separation from an adjacent module . The full-length PglA and five truncated derivatives containing one or two modules were constructed, recombinantly produced, purified and characterized. The function of each module referring to carbohydrate-binding and hydrolyzing properties was identified respectively. Specific activities of PglA and truncated derivatives on various substrates were measured. The strict and selective specificity of PglA suggested that it was a strict β-1,3-glucanase belonging to EC 220.127.116.11 family of glycoside hydrolases. The truncated mutant PglA-CD, which contained only the catalytic domain, showed reduced hydrolytic activity on insoluble substrates, while, its activity remained unchanged on soluble substrates (Table 4). It could be suggested that the N- or C-terminal domain did not play a direct or critical role in the structural stability of the catalytic domain. The results of binding assay demonstrated that both the N-and C-terminal domains of PglA had significant binding activities toward insoluble linear β-1,3-glucan (pachyman) and branched-chain β-1,3-glucan (zymosan A) respectively (Figure 5), suggesting their module functions in the enzymatic activity of PglA. It was in accordance with the β-1,3-glucanase (BglH) from Bacillus circulans IAM1165 . Amino acid sequence alignment showed no significant similarity between the N-and C-terminal domains. Thus, both of them could be classified into CBM families respectively. Either N-or C-terminal CBM could exert the strong binding toward insoluble β-1,3-glucan (pachyman and zymosan A), which probably account for the increase of the specific activities of rPglA∆C or rPglA∆N against these insoluble substrates, presumably by assisting the localization of catalytic domains to insoluble polysaccharides.
The full-length PglA and truncated glucanases (PglA∆C, PglA∆N and PglA-CD) exhibited similar enzymatic properties. The pH activity and stability profiles of rPglA and the truncated enzymes were nearly indistinguishable, except that rPglA∆C retained higher activity in acidic range of the pH spectrum (pH 2.0-4.5) than the other deletion derivatives. The results of thermal activity and stability assay indicated the relatively low influence of the N-terminal CBM on enzymatic properties of PglA. However, deletion of the C-terminus in PglA, consisting of an Ig-like fold and a basic terminal domain, significantly increased the thermal tolerance of the enzyme, which was first observed with bacterial β-1,3-glucanase. These observations imply the negative impact of the C-terminal CBM on enzymatic acidic and thermal stabilities of catalytic domain. A similar negative effect has been identified for endo-β-1,4-glucanase from Bacillus subtilis JA18 in that truncation of the cellulose binding domain improved thermal stability of the enzyme . Carbohydrate binding is not the sole function of the non-catalytic modules . The impact of CBMs on enzymatic thermal stability of a multidomain xylanase has been discovered by Winterhalter et al. in 1995 . Increasing literature showed controversial data on CBMs affecting glycoside hydrolase’s thermostability. The comparison of catalytic module alone with catalytic module fused to CBM showed either improvement of thermostability [36–38], or on the contrary appeared to have a negative impact [35, 39]. In this report, the negative impact of C-terminal CBM containing an Ig-like structure on thermostability as well as acidic stability of β-1,3-glucanase was revealed.
Proteins possessing the Ig-like fold are commonly found in bacteria, and are most often involved in cell-cell adhesion, or extracellular glycohydrolysis . Recent years, there is increasing structural information on the existence of these domains in glycoside hydrolase family proteins. Ig-like domain is generally considered being involved in carbohydrate binding and assist in hydrolysis reactions for insoluble substrates . The influence of Ig-like domain on enzymatic stability and activity of GH family proteins has been mainly illustrated in cellulose hydrolases. Liu et al. revealed that deletion of the C-terminal Ig-like domains in GH5 family cellulose resulted in a significant loss of enzymatic activity toward soluble and insoluble cellulose substrates . Many members of family 9 of glycoside hydrolases have an N-terminal Ig-like domain which is responsible for the hydrolytic activity and stability of the catalytic domain [33, 42]. Tight structural interaction between the Ig-like and GH9 domains was evidenced by analyzing the crystal structure of the Ig-GH9 module pair . It can be inferred that the C-terminal Ig-like structure also physically associate with the active site of the catalytic domain of PglA and hampered the thermal activity and stability of the enzyme. In contrast, the negligible impact of the N-terminal peptide on enzymatic stability and activity indicated it is very likely to fold as an independent structural module and show little interactions with the catalytic module of PglA.
In the present study, a new β-1,3-glucanase gene PglA was cloned from Paenibacillus sp. S09. Truncation forms of PglA were successfully expressed in E.coli. Both the N-and C-terminal non-catalytic domains exerted carbohydrate-binding functions, while the C-terminal region containing an Ig-like fold had negative impact on thermostability of catalytic domain. There is increasing documentation that the CBMs of bacterial glycoside hydrolases containing Ig-like structure. As the first report describing the characterization of a GH16 β-1,3-glucanase with an C-terminus Ig-like region, the present study improved our understanding on the module relationships between the enzyme’s catalytic and non-catalytic domains and could provide information that may suggest an approach to rationally modify the characteristics of an enzyme for industrial application.
Glycoside hydrolase family 16
Self-formed adaptor PCR
Full-length Paenibacillus sp. S09 β-1,3-glucanase
C-terminus truncated PglA
N-terminus truncated PglA
Catalytic domain of PglA
N-terminal domain of PglA
C-terminal domain of PglA.
Zverlov VV, Volkov IY, Velikodvorskaya TV, Schwarz WH: Highly thermostable endo-1,3-β-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gene and characterization of the recombinant gene product. Microbiology. 1997, 143: 1701-1708. 10.1099/00221287-143-5-1701.
Henrissat B: A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J. 1991, 280: 309-316.
De la Cruz J, Pintor-Toro JA, Benítez T, Llobell A, Romero LC: A novel endo-β-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum. J Bacteriol. 1995, 177: 6937-6945.
Liu B, Xue X, Cui S, Zhang X, Han Q, Zhu L, Liang X, Wang X, Huang L, Chen X, Kang Z: Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici. Mol Biol Rep. 2010, 37: 1045-1052. 10.1007/s11033-009-9823-9.
Bielecki S, Galas E: Microbial β-glucanases different from cellulases. Crit Rev Biotechnol. 1991, 10: 275-304. 10.3109/07388559109038212.
Gacto M, Vicente-Soler J, Cansado J, Villa TG: Characterization of an extracellular enzyme system produced by Micromonospora chalcea with lytic activity on yeast cells. J Appl Microbiol. 2000, 88: 961-967. 10.1046/j.1365-2672.2000.01065.x.
Shi P, Yao G, Yang P, Li N, Luo H, Bai Y, Wang Y, Yao B: Cloning, characterization, and antifungal activity of an endo-1,3-β-D-glucanase from Streptomyces sp. S27. Appl Microbiol Biotechnol. 2010, 85: 1483-1490. 10.1007/s00253-009-2187-1.
Blättel V, Larisika M, Pfeiffer P, Nowak C, Eich A, Eckelt J, König H: β-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification. Appl Environ Microbiol. 2011, 77: 983-990. 10.1128/AEM.01943-10.
Miyanishi N, Iwamoto Y, Watanabe E, Odaz T: Induction of TNF-α production from human peripheral blood monocytes with β-1,3-glucan oligomer prepared from laminarin with β-1,3-glucanase from Bacillus clausii NM-1. J Biosci Bioeng. 2003, 95: 192-195.
O’Connell E, Piggott C, Tuohy M: Purification of exo-1,3-β-glucanase, a new extracellular glucanolytic enzyme from Talaromyces emersonii. Appl Microbiol Biotechnol. 2011, 89: 685-696. 10.1007/s00253-010-2883-x.
Yamamoto M, Aono R, Horikoshi K: Structure of the 87-kDa β-1,3-glucanase gene of Bacillus circulans IAM1165 and properties of the enzyme accumulated in the periplasm of Escherichia coli carrying the gene. Biosci Biotechnol Biochem. 1993, 57: 1518-1525. 10.1271/bbb.57.1518.
Hong TY, Meng M: Biochemical characterization and antifungal activity of an endo-1,3-β-glucanase of Paenibacillus sp. isolated from garden soil. Appl Microbiol Biotechnol. 2003, 61: 472-478.
Cheng YM, Hong TY, Liu CC, Meng M: Cloning and functional characterization of a complex endo-β-1,3-glucanase from Paenibacillus sp. Appl Microbiol Biotechnol. 2009, 81: 1051-1061. 10.1007/s00253-008-1617-9.
Tanabe Y, Oda M: Molecular characterization of endo-1,3-β-glucanase from Cellulosimicrobium cellulans: effects of carbohydrate-binding module on enzymatic function and stability. Biochim Biophys Acta. 1814, 2011: 1713-1719.
Palumbo JD, Sullivan RF, Kobayashi DY: Molecular characterization and expression in Escherichia coli of three β-1,3-glucanase genes from Lysobacter enzymogenes strain N4-7. J Bacteriol. 2003, 185: 4362-4370. 10.1128/JB.185.15.4362-4370.2003.
Xiu A, Kong Y, Zhou M, Zhu B, Wang S, Zhang J: The chemical and digestive properties of a soluble glucan from Agrobacterium sp. ZX09. Carbohyd Polym. 2010, 82: 623-628. 10.1016/j.carbpol.2010.05.027.
Sambrook J, Russell DW: Molecular cloning: a laboratory manual. 2001, New York: Cold Spring Harbor Laboratory Press, Cold Spring Har
Wang S, He J, Cui Z, Li S: Self-formed adaptor PCR: a simple and efficient method for chromosome walking. Appl Environ Microbiol. 2007, 73: 5048-5051. 10.1128/AEM.02973-06.
Petersen TN, Brunak S, Von Heijne G, Nielsen H: SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods. 2011, 8: 785-786. 10.1038/nmeth.1701.
Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Lu S, Marchler GH, Song JS, Thanki N, Yamashita RA, Zhang D, Bryant SH: CDD: conserved domains and protein three-dimensional structure. Nucleic Acids Res. 2013, 41: 348-352. 10.1093/nar/gks1243.
Buchan DW, Ward SM, Lobley AE, Nugent TC, Bryson K, Jones DT: Protein annotation and modelling servers at University College London. Nucleic Acids Res. 2010, 38: W563-W568. 10.1093/nar/gkq427.
Arnold K, Bordoli L, Kopp J, Schwede T: The SWISS-MODEL workspace:a web-based environment for protein structure homology modelling. Bioinformatics. 2006, 22: 195-201. 10.1093/bioinformatics/bti770.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal X version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.
Kimura M: A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980, 16: 111-120. 10.1007/BF01731581.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011, 28: 2731-2739. 10.1093/molbev/msr121.
Miller GL: Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959, 31: 426-428. 10.1021/ac60147a030.
Yamamoto M, Ezure T, Watanabe T, Tanaka H, Aono R: C-terminal domain of β-1,3-glucanase H in Bacillus circulans IAM1165 has a role in binding to insoluble β-1,3-glucan. FEBS Lett. 1998, 433: 41-43. 10.1016/S0014-5793(98)00881-3.
Cheng YM, Hsieh FC, Meng M: Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus β-1,3-glucanase. Microb Cell Fact. 2009, 8: 62-10.1186/1475-2859-8-62.
Van Bueren AL, Morland C, Gilbert HJ, Boraston AB: Family 6 carbohydrate binding modules recognize the non-reducing end of β-1,3-linked glucans by presenting a unique ligand binding surface. J Biol Chem. 2005, 280: 530-537.
Asano T, Taki J, Yamamoto M, Aono R: Cloning and structural analysis of bglM gene coding for the fungal cell wall-lytic β-1,3-glucan-hydrolase BglM of Bacillus circulans IAM1165. Biosci Biotechnol Biochem. 2002, 66: 1246-1255. 10.1271/bbb.66.1246.
Dvortsov IA, Lunina NA, Chekanovskaya LA, Schwarz WH, Zverlov VV, Velikodvorskaya GA: Carbohydrate-binding properties of a separately folding protein module from β-1,3-glucanase Lic16A of Clostridium thermocellum. Microbiology. 2009, 155: 2442-2449. 10.1099/mic.0.026930-0.
Koizumi N, Masuda S, Maeda K, Isoda Y, Yatsunami R, Fukui T, Nakamura S: Additional carbohydrate-binding modules enhance the insoluble substrate-hydrolytic activity of β-1,3-glucanase from alkaliphilic Nocardiopsis sp. F96. Biosci Biotechnol Biochem. 2009, 73: 1078-1082. 10.1271/bbb.80846.
Liu H, Pereira JH, Adams PD, Sapra R, Simmons BA, Sale KL: Molecular simulations provide new insights into the role of the accessory immunoglobulin-like domain of Cel9A. FEBS Lett. 2010, 584: 3431-3435. 10.1016/j.febslet.2010.06.041.
Araki R, Ali MK, Sakka M, Kimura T, Sakka K, Ohmiya K: Essential role of the family-22 carbohydrate-binding modules for β-1,3-1,4-glucanase activity of Clostridium stercorarium Xyn10B. FEBS Lett. 2004, 561: 155-158. 10.1016/S0014-5793(04)00160-7.
Wang Y, Yuan H, Wang J, Yu Z: Truncation of the cellulose binding domain improved thermal stability of endo-β-1,4-glucanase from Bacillus subtilis JA18. Bioresour Technol. 2009, 100: 345-349. 10.1016/j.biortech.2008.06.001.
Verjans P, Dornez E, Segers M, Van Campenhout S, Bernaerts K, Beliën T, Delcour JA, Courtin CM: Truncated derivatives of a multidomain thermophilic glycosyl hydrolase family 10 xylanase from Thermotoga maritima reveal structure related activity profiles and substrate hydrolysis patterns. J Biotechnol. 2010, 145: 160-167. 10.1016/j.jbiotec.2009.10.014.
Winterhalter C, Heinrich P, Candussio A, Wich G, Liebl W: Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacterium Thermotoga maritima. Mol Microbiol. 1995, 15: 431-444. 10.1111/j.1365-2958.1995.tb02257.x.
Couturier M, Feliu J, Haon M, Navarro D, Lesage-Meessen L, Coutinho PM, Berrin JG: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity. Microb Cell Fact. 2011, 10: 103-10.1186/1475-2859-10-103.
Selvaraj T, Kim SK, Kim YH, Jeong YS, Kim YJ, Phuong ND, Jung KH, Kim J, Yun HD, Kim H: The role of carbohydrate-binding module (CBM) repeat of a multimodular xylanase (XynX) from Clostridium thermocellum in cellulose and xylan binding. J Microbiol. 2010, 48: 856-861. 10.1007/s12275-010-0285-5.
Fraser JS, Yu Z, Maxwell KL, Davidson AR: Ig-like domains on bacteriophages: a tale of promiscuity and deceit. J Mol Biol. 2006, 359: 496-507. 10.1016/j.jmb.2006.03.043.
Liu G, Qin Y, Hu Y, Gao M, Peng S, Qu Y: An endo-1,4-β-glucanase PdCel5C from cellulolytic fungus Penicillium decumbens with distinctive domain composition and hydrolysis product profile. Enzyme Microb Technol. 2013, 52: 190-195. 10.1016/j.enzmictec.2012.12.009.
Kataeva IA, Uversky VN, Brewer JM, Schubot F, Rose JP, Wang BC, Ljungdahl LG: Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA. Protein Eng Des Sel. 2004, 17: 759-769. 10.1093/protein/gzh094.
This work was funded by 973 Program 2013CB945203, JSSTP BE2011836 and NSFC31071130.
All of authors declare no competing interests.
RC performed the cloning, expression and purification experiments, analyzed the data and was involved in drafting the manuscript. JPC and XHY carried out the enzyme activity assays. SMW participated in the sequence analysis and truncation design. YW assisted with sequence analysis and enzyme characterization. JFZ contributed to study conception and design, analysis and interpretation of data, as well as drafting and revision of the manuscript, and acquisition of funding. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: Figure S1: Conserved domain sequence alignment of GH16 endo-β-1,3-glucanases. The organisms and accession numbers are as follows: Nocardiopsis sp.F96 (BAE54302.1), Cellulosimicrobium cellulans (AAC44371.1), Paenibacillus mucilaginosus KNP414 (YP_004643413.1), Bacillus clausii KSM-K16 (YP_174203.1), Bacillus circulans IAM1165 (BAA04469.1), Paenibacillus sp.CCRC 17245 (ABJ15796.1), Phaeodactylum tricornutum CCAP 1055/1 (XP_002181321.1). The most conserved regions were used for degenerated primers. Identical amino acids are indicated by solid black and gray. The GH16 active site and two glutamate residues are indicated by frame and asterisks respectively. (PDF 230 KB)
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Cheng, R., Chen, J., Yu, X. et al. Recombinant production and characterization of full-length and truncated β-1,3-glucanase PglA from Paenibacillussp. S09. BMC Biotechnol 13, 105 (2013). https://doi.org/10.1186/1472-6750-13-105
- Ig-like domain