Table 3 Purification of rPglA and its truncated derivatives
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
rPglA | |||||
Crude extract | 2.56 | 9.80 | 0.26 | 100 | 1 |
Ni2 + −affinity column | 1.43 | 0.72 | 1.99 | 58.1 | 7.62 |
rPglA∆C | |||||
Crude extract | 4.01 | 12.4 | 0.32 | 100 | 1 |
Ni2 + −affinity column | 2.21 | 1.05 | 2.14 | 55.1 | 6.63 |
rPglA∆N | |||||
Crude extract | 2.85 | 9.71 | 0.29 | 100 | 1 |
Ni2 + −affinity column | 1.53 | 0.75 | 2.04 | 53.7 | 6.96 |
rPglA-CD | |||||
Crude extract | 2.51 | 8.81 | 0.29 | 100 | 1 |
Ni2 + −affinity column | 1.05 | 0.60 | 1.76 | 42.1 | 6.17 |