Table 1 Primers used for the cloning of β-1,3-glucanase gene
Primer name | Primer sequence (5′ → 3′)a |
|---|---|
Pdg-F | TAY ACS CGS ACR TAR TC |
Pdg-R | GAY TAY GTS CGS GTR TA |
gIF1 | GTAGCGGTTGACCGGCCATTGG |
gIF2 | GCTGCTGTTCGCCTTGGAGT |
gIR1 | CGACCAGCCGTTCTATCTGATCAT |
gIR2 | GGCGACGATGCAGGTAGACTATGT |
5′-sp1 | CGTTTCATTGACATTCTGGAAGACGAGCGT |
5′-sp2 | TGTAGTTGAGTGTGACGCTGCTGTTCGC |
5′-hemi-sp3 | AATGTCGACCCAGCTNNNNNNNNNCTTGAC |
3′-sp1 | AGAGATCGACATCATGGAGGCGAAGGG |
3′-sp2 | TACCTCCCGCAAGGTACGACATTCG |
3′-hemi-sp3 | ACCAGCCGTTCTATCNNNNNNNNAACCTG |