Recombinant production and characterization of full-length and truncated β-1,3-glucanase PglA from Paenibacillussp. S09

Table 4 Substrate specificity of rPglA and its truncated derivatives

Substrate^a	Solubility	Main linkage		Specific activity (U/mg)^bof:
			rPglA	rPglA∆C	rPglA∆N	rPglA-CD
Laminarin	Soluble	β-1,3, β-1,6 (Glc)	2.016 ± 0.213	2.506 ± 0.131	2.000 ± 0.099	2.525 ± 0.141
Pachyman	Insoluble	β-1,3 (Glc)	1.695 ± 0.165	2.396 ± 0.339	1.883 ± 0.045	0.898 ± 0.078
Zymosan A	Insoluble	β-1,3, β-1,6 (Glc)	1.364 ± 0.131	1.518 ± 0.036	0.835 ± 0.022	0.567 ± 0.035
Barley β-glucan	Soluble	β-1,3, β-1,4 (Glc)	0.086 ± 0.011	0.074 ± 0.001	0.076 ± 0.021	0.081 ± 0.015
Salecan	Soluble	β-1,3, α-1,3 (Glc)	0.049 ± 0.010	0.039 ± 0.005	0.037 ± 0.009	0.032 ± 0.007
CMC	Soluble	β-1,4 (Glc)	0	0	0	0
Dextran	Soluble	α-1,6 (Glc)	0	0	0	0
xylan	Insoluble	β-1,4 (xyl)	0	0	0	0

^aAll substrates were used at a final concentration of 10 mg/ml, except salecan (5 mg/ml).
^bActivity ± standard error of triplicate samples.

ISSN: 1472-6750