Skip to main content

Advertisement

Articles

Page 1 of 30

  1. Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate...

    Authors: Yanqin Xu, Junjiang Zhou, Qingqing Liu, Kunpeng Li and Yin Zhou

    Citation: BMC Biotechnology 2020 20:4

    Content type: Research article

    Published on:

  2. Insulin controls hyperglycemia caused by diabetes, and virtually all treatments require exogenous insulin. However, the product’s extensive post-translational modifications have hindered the manufacture of rec...

    Authors: Minmin Zhang, Yunlong Zhang, Bingnan Wu, Yanhao Peng, Altaf Ahmed Simair, Geoffery W. Siegel, Changrui Lu and Ting Chen

    Citation: BMC Biotechnology 2020 20:3

    Content type: Research article

    Published on:

  3. Cellulosic biomass degradation still needs to be paid more attentions as bioenergy is the most likely to replace fossil energy in the future, and more evaluable cellulolytic bacteria isolation will lay a found...

    Authors: Lingling Ma, Yingying Lu, Hong Yan, Xin Wang, Yanglei Yi, Yuanyuan Shan, Bianfang Liu, Yuan Zhou and Xin Lü

    Citation: BMC Biotechnology 2020 20:2

    Content type: Research article

    Published on:

  4. Interferon-gamma (IFN-γ) is an important mediator of type I immune response and has antiviral, immunoregulatory and anti-tumor properties, plays a wide range of roles in inflammation and autoimmune diseases. T...

    Authors: Wen-Tao Ma, Qi Liu, Meng-Xia Ning, Yu-Xu Qi, Saad Rehman and De-Kun Chen

    Citation: BMC Biotechnology 2019 19:102

    Content type: Research article

    Published on:

  5. Clostridium perfringens, a gram-positive, anaerobic, rod-shaped bacterium, is the third leading cause of human foodborne bacterial disease and a cause of necrotic enteritis in poultry. It is controlled using anti...

    Authors: Rosemarie W. Hammond, Steven M. Swift, Juli A. Foster-Frey, Natalia Y. Kovalskaya and David M. Donovan

    Citation: BMC Biotechnology 2019 19:101

    Content type: Research article

    Published on:

  6. Bacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates. These libraries typically express a scaffold protein embedded within ...

    Authors: Sarah D. Stellwagen, Deborah A. Sarkes, Bryn L. Adams, Mia A. Hunt, Rebecca L. Renberg, Margaret M. Hurley and Dimitra N. Stratis-Cullum

    Citation: BMC Biotechnology 2019 19:100

    Content type: Research Article

    Published on:

  7. To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality o...

    Authors: Min Zhang, Min Wei, Zhiguo Dong, Haibao Duan, Shuang Mao, Senlei Feng, Wenqian Li, Zepeng Sun, Jiawei Li, Kanglu Yan, Hao Liu, Xueping Meng and Hongxing Ge

    Citation: BMC Biotechnology 2019 19:99

    Content type: Research article

    Published on:

  8. Wolbachia pipientis is a widespread, obligatory intracellular and maternally inherited bacterium, that induces a wide range of reproductive alterations to its hosts. Cytoplasmic Incompatibility (CI) is causing em...

    Authors: Georgios A. Kyritsis, Antonios A. Augustinos, Ioannis Livadaras, Carlos Cáceres, Kostas Bourtzis and Nikos T. Papadopoulos

    Citation: BMC Biotechnology 2019 19(Suppl 2):96

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  9. The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the S...

    Authors: Lucas Alexander Shuttleworth, Mohammed Abul Monjur Khan, Terrence Osborne, Damian Collins, Mukesh Srivastava and Olivia Louise Reynolds

    Citation: BMC Biotechnology 2019 19(Suppl 2):95

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  10. The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is an important polyphagous pest of horticultural produce. The sterile insect technique (SIT) is a proven control method against many i...

    Authors: Mahfuza Khan, Kajla Seheli, Md. Abdul Bari, Nahida Sultana, Shakil Ahmed Khan, Khandokar Fahmida Sultana and Md. Anwar Hossain

    Citation: BMC Biotechnology 2019 19(Suppl 2):94

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  11. The olive fly, Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research on B. oleae control methods, scientists ...

    Authors: Gaia Bigiotti, Roberta Pastorelli, Roberto Guidi, Antonio Belcari and Patrizia Sacchetti

    Citation: BMC Biotechnology 2019 19(Suppl 2):93

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  12. The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval devel...

    Authors: Doron Shalom Yishai Zaada, Michael Ben-Yosef, Boaz Yuval and Edouard Jurkevitch

    Citation: BMC Biotechnology 2019 19(Suppl 2):92

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  13. The symbiosis between the olive fruit fly, Bactrocera oleae, and Candidatus Erwinia dacicola has been demonstrated as essential for the fly’s larval development and adult physiology. The mass rearing of the olive...

    Authors: Patrizia Sacchetti, Roberta Pastorelli, Gaia Bigiotti, Roberto Guidi, Sara Ruschioni, Carlo Viti and Antonio Belcari

    Citation: BMC Biotechnology 2019 19(Suppl 2):91

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  14. Enterobacter sp. AA26 was recently isolated from the midgut of Ceratitis capitata (Wiedemann) and it was shown to have positive effects in rearing efficiency when used as larval probiotics. In this study, biomass...

    Authors: Konstantinos Azis, Ioanna Zerva, Paraschos Melidis, Carlos Caceres, Kostas Bourtzis and Spyridon Ntougias

    Citation: BMC Biotechnology 2019 19(Suppl 2):90

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  15. Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its ...

    Authors: Kala Bhandari, Peter Crisp and Michael A. Keller

    Citation: BMC Biotechnology 2019 19(Suppl 2):89

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  16. Symbiotic bacteria contribute to a multitude of important biological functions such as nutrition and reproduction and affect multiple physiological factors like fitness and longevity in their insect hosts. The...

    Authors: Elias D. Asimakis, Mahfuza Khan, Panagiota Stathopoulou, Carlos Caceres, Kostas Bourtzis and George Tsiamis

    Citation: BMC Biotechnology 2019 19(Suppl 2):88

    Content type: Research

    Published on:

    This article is part of a Supplement: Volume 19 Supplement 2

  17. Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, R...

    Authors: Wenchao Chen, Xuan Li, Xuli Ma, Shouwen Chen, Yanping Kang, Minmin Yang, Fenghong Huang and Xia Wan

    Citation: BMC Biotechnology 2019 19:98

    Content type: Research article

    Published on:

  18. Immunogold labeling in combination with transmission electron microscopy analysis is a technique frequently used to correlate high-resolution morphology studies with detailed information regarding localization...

    Authors: Tohidul Islam, Anna L. Gharibyan, Cheng Choo Lee and Anders Olofsson

    Citation: BMC Biotechnology 2019 19:97

    Content type: Methodology article

    Published on:

  19. The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of close...

    Authors: Xiaoyang Pang, Ziyang Jia, Jing Lu, Shuwen Zhang, Cai Zhang, Min Zhang and Jiaping Lv

    Citation: BMC Biotechnology 2019 19:87

    Content type: Research article

    Published on:

  20. The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensiv...

    Authors: Jie Dong, Xiangzhen Ding and Sheng Wang

    Citation: BMC Biotechnology 2019 19:86

    Content type: Research article

    Published on:

  21. The invasive fruit pest Drosophila suzukii was reported for the first time in Europe and the USA in 2008 and has spread since then. The adoption of type II clustered regularly interspaced short palindromic repeat...

    Authors: Hassan M. M. Ahmed, Luisa Hildebrand and Ernst A. Wimmer

    Citation: BMC Biotechnology 2019 19:85

    Content type: Research article

    Published on:

  22. Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by ...

    Authors: Noah H. Richardson, Jordan B. Luttrell, Jonathan S. Bryant, Damian Chamberlain, Saleem Khawaja, Indira Neeli and Marko Radic

    Citation: BMC Biotechnology 2019 19:84

    Content type: Review

    Published on:

  23. The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are bein...

    Authors: Khadega A. Almaqdi, Rana Morsi, Bahia Alhayuti, Farah Alharthi and S. Salman Ashraf

    Citation: BMC Biotechnology 2019 19:83

    Content type: Research article

    Published on:

  24. Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secret...

    Authors: Innanurdiani Koko, Adelene Ai-Lian Song, Mas Jaffri Masarudin and Raha Abdul Rahim

    Citation: BMC Biotechnology 2019 19:82

    Content type: Research article

    Published on:

  25. Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ...

    Authors: Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans and Anne-Catherine Servais

    Citation: BMC Biotechnology 2019 19:81

    Content type: Research article

    Published on:

  26. Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell...

    Authors: Yong Xu Mu, Yu Xia Zhao, Bing Yao Li, Hong Jing Bao, Hui Jiang, Xiao Lei Qi, Li Yun Bai, Yun Hong Wang, Zhi Jie Ma and Xiao Yun Wu

    Citation: BMC Biotechnology 2019 19:80

    Content type: Methodology article

    Published on:

  27. Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p...

    Authors: Elinea O. Freitas, Bruno P. Melo, Isabela T. Lourenço-Tessutti, Fabrício B. M. Arraes, Regina M. Amorim, Maria E. Lisei-de-Sá, Julia A. Costa, Ana G. B. Leite, Muhammad Faheem, Márcio A. Ferreira, Carolina V. Morgante, Elizabeth P. B. Fontes and Maria F. Grossi-de-Sa

    Citation: BMC Biotechnology 2019 19:79

    Content type: Research article

    Published on:

  28. Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. H...

    Authors: Yingshuo Hou, Siyu Chen, Jianjun Wang, Guizhen Liu, Sheng Wu and Yong Tao

    Citation: BMC Biotechnology 2019 19:76

    Content type: Research article

    Published on:

  29. DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transge...

    Authors: Kristian Alsbjerg Skipper, Anne Kruse Hollensen, Michael N. Antoniou and Jacob Giehm Mikkelsen

    Citation: BMC Biotechnology 2019 19:75

    Content type: Research article

    Published on:

  30. Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated...

    Authors: Guoxing Zheng, Qingqing Zhu, Junchao Dong, Xin Lin and Chengming Zhu

    Citation: BMC Biotechnology 2019 19:74

    Content type: Research article

    Published on:

  31. Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assa...

    Authors: Aurélie Bak and Joanne B. Emerson

    Citation: BMC Biotechnology 2019 19:73

    Content type: Methodology article

    Published on:

  32. In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the me...

    Authors: Lei Yang, Tore Linde, Abeer H. Hossain, Mette Lübeck, Peter J. Punt and Peter S. Lübeck

    Citation: BMC Biotechnology 2019 19:72

    Content type: Research article

    Published on:

  33. The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity...

    Authors: Taj Arndell, Niharika Sharma, Peter Langridge, Ute Baumann, Nathan S. Watson-Haigh and Ryan Whitford

    Citation: BMC Biotechnology 2019 19:71

    Content type: Research article

    Published on:

  34. Aspergillus oryzae, a useful industrial filamentous fungus, produces limited varieties of secondary metabolites, such as kojic acid. Thus, for the production of valuable secondary metabolites by genetic engineeri...

    Authors: Koichi Tamano, Mahoko Kuninaga, Naoshi Kojima, Myco Umemura, Masayuki Machida and Hideaki Koike

    Citation: BMC Biotechnology 2019 19:70

    Content type: Research article

    Published on:

  35. Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the resear...

    Authors: Prassan Choudhary, Sudipta Das, Hillol Chakdar, Arjun Singh, Sanjay Kumar Goswami and Anil Kumar Saxena

    Citation: BMC Biotechnology 2019 19:69

    Content type: Methodology article

    Published on:

  36. Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the ze...

    Authors: David Simon Kleinhans and Virginie Lecaudey

    Citation: BMC Biotechnology 2019 19:68

    Content type: Methodology article

    Published on:

  37. Lymphocyte-activation gene (LAG)3 is a 498 aa transmembrane type I protein acting as an immune inhibitory receptor. It is expressed on activated lymphocytes, natural killer cells and plasmacytoid dendritic cel...

    Authors: Alessandro Ascione, Claudia Arenaccio, Alessandra Mallano, Michela Flego, Mara Gellini, Mauro Andreotti, Craig Fenwick, Giuseppe Pantaleo, Stefano Vella and Maurizio Federico

    Citation: BMC Biotechnology 2019 19:67

    Content type: Research article

    Published on:

  38. Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybe...

    Authors: Di Qin, Xiao-Yi Liu, Cristina Miceli, Qi Zhang and Pi-wu Wang

    Citation: BMC Biotechnology 2019 19:66

    Content type: Research article

    Published on:

  39. Ebola hemorrhagic fever is caused by the Ebola filovirus (EBOV), which is one of the most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subv...

    Authors: Michela Flego, Aldo Frau, Luisa Accardi, Alessandra Mallano, Alessandro Ascione, Mara Gellini, Elisa Fanunza, Stefano Vella, Paola Di Bonito and Enzo Tramontano

    Citation: BMC Biotechnology 2019 19:64

    Content type: Research article

    Published on:

  40. In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to...

    Authors: Jian Pang, Junshu Wang, Zhanying Liu, Qiancheng Zhang and Qingsheng Qi

    Citation: BMC Biotechnology 2019 19:63

    Content type: Research article

    Published on:

  41. In personalized medicine, companion diagnostic tests provide additional information to help select a treatment option likely to be optimal for a patient. Although such tests include several techniques for dete...

    Authors: Jr-Kai Huang, Ling Fan, Tao-Yeuan Wang and Pao-Shu Wu

    Citation: BMC Biotechnology 2019 19:62

    Content type: Methodology article

    Published on:

    The Correction to this article has been published in BMC Biotechnology 2019 19:77

  42. Laccases are multicopper oxidases, which are assigned into auxiliary activity family 1 (AA1) in the CAZy database. These enzymes, catalyzing the oxidation of phenolic and nonphenolic substrates coupled to redu...

    Authors: Majid Haddad Momeni, Paolo Bollella, Roberto Ortiz, Esben Thormann, Lo Gorton and Maher Abou Hachem

    Citation: BMC Biotechnology 2019 19:61

    Content type: Research article

    Published on:

  43. To directly assess the biological role of oligosaccharides in recombinant equine chorionic gonadotropin (rec-eCG) functioning, cDNA encoding the full-length eCGβ-subunit was fused with the mature protein part ...

    Authors: Kwan-Sik Min, Jong-Ju Park, Munkhzaya Byambaragchaa and Myung-Hwa Kang

    Citation: BMC Biotechnology 2019 19:60

    Content type: Research article

    Published on:

  44. Heparinase I from Pedobacter heparinus (Ph-HepI), which specifically cleaves heparin and heparan sulfate, is one of the most extensively studied glycosaminoglycan lyases. Enzymatic degradation of heparin by hepar...

    Authors: Chuan Zhang, Bao-Cheng Yang, Wen-Ting Liu, Zhong-Yuan Li, Ya-Jian Song, Tong-Cun Zhang and Xue-Gang Luo

    Citation: BMC Biotechnology 2019 19:59

    Content type: Research article

    Published on:

  45. Efficient xylose fermentation still demands knowledge regarding xylose catabolism. In this study, metabolic flux analysis (MFA) and metabolomics were used to improve our understanding of xylose metabolism. Thu...

    Authors: Henrique C. T. Veras, Christiane G. Campos, Igor F. Nascimento, Patrícia V. Abdelnur, João R. M. Almeida and Nádia S. Parachin

    Citation: BMC Biotechnology 2019 19:58

    Content type: Research article

    Published on:

  46. The textural characteristics of fermented dairy products are important quality parameters that play a major role in their stability and consumer’s acceptance. The aim of this study was to investigate the influ...

    Authors: Soumaya El Bouchikhi, Philippe Pagès, Yassir El Alaoui, Azeddine Ibrahimi and Yahya Bensouda

    Citation: BMC Biotechnology 2019 19:57

    Content type: Research article

    Published on:

  47. Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main ad...

    Authors: Thomas Thisted, Zuzana Biesova, Celine Walmacq, Everett Stone, Max Rodnick-Smith, Shaheda S. Ahmed, Stephen K. Horrigan, Bo Van Engelen, Charles Reed and Matthew W. Kalnik

    Citation: BMC Biotechnology 2019 19:56

    Content type: Research article

    Published on: