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  1. The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo sele...

    Authors: Jijumon Chelliserrykattil, George Cai and Andrew D Ellington
    Citation: BMC Biotechnology 2001 1:13
  2. Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause lethality because lines of founder mi...

    Authors: Ravi P Misra, Sarah K Bronson, Qi Xiao, Wendy Garrison, Jixuan Li, Roong Zhao and Stephen A Duncan
    Citation: BMC Biotechnology 2001 1:12
  3. Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and ...

    Authors: Larry R Karns PhD, Anne Kisielewski BSc, Kathryn M Gulding BSc and Dan Theodorescu MD PhD
    Citation: BMC Biotechnology 2001 1:11
  4. To maintain populations of microbial cells under controlled conditions of growth and environment for an indefinite duration is a prerequisite for experimentally evolving natural isolates of wild-type species o...

    Authors: Valérie A de Crécy-Lagard, Jacques Bellalou, Rupert Mutzel and Philippe Marlière
    Citation: BMC Biotechnology 2001 1:10
  5. Modern drug discovery is concerned with identification and validation of novel protein targets from among the 30,000 genes or more postulated to be present in the human genome. While protein-protein interactio...

    Authors: Renuka C Pillutla, Ku-chuan Hsiao, Renee Brissette, Paul S Eder, Tony Giordano, Paul W Fletcher, Michael Lennick, Arthur J Blume and Neil I Goldstein
    Citation: BMC Biotechnology 2001 1:6
  6. There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However...

    Authors: Jae Hong Im, Takashi Nakane, Hiroshi Yanagishita, Toru Ikegami and Dai Kitamoto
    Citation: BMC Biotechnology 2001 1:5
  7. Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromol...

    Authors: Vadim V Shmanai, Tamara A Nikolayeva, Ludmila G Vinokurova and Anatoli A Litoshka
    Citation: BMC Biotechnology 2001 1:4
  8. The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new a...

    Authors: Manuel Rodríguez Valle, Carlos Montero, Héctor Machado and Marisdania Joglar
    Citation: BMC Biotechnology 2001 1:2
  9. Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli i...

    Authors: Agata Czyz, Marcin Los, Borys Wrobel and Grzegorz Wegrzyn
    Citation: BMC Biotechnology 2001 1:1

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