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  1. Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR)....

    Authors: Sanum Bashir, Tu Dang, Jana Rossius, Johanna Wolf and Ralf Kühn

    Citation: BMC Biotechnology 2020 20:57

    Content type: Research article

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  2. Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a ...

    Authors: Shohreh Ariaeenejad, Atefeh Sheykh Abdollahzadeh Mamaghani, Morteza Maleki, Kaveh Kavousi, Mehdi Foroozandeh Shahraki and Ghasem Hosseini Salekdeh

    Citation: BMC Biotechnology 2020 20:56

    Content type: Research article

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  3. Buriti oil presents numerous health benefits, but due to its lipophilic nature and high oxidation, it is impossible to incorporate it into aqueous food matrices. Thus, the present study evaluated whether powde...

    Authors: Gabrielle Mahara Martins Azevedo Castro, Thais Souza Passos, Sara Sayonara da Cruz Nascimento, Isaiane Medeiros, Nathália Kelly Araújo, Bruna Leal Lima Maciel, Carlos Eduardo Padilha, Adriana Margarida Zanbotto Ramalho, Francisco Canidé Sousa Júnior and Cristiane Fernandes de Assis

    Citation: BMC Biotechnology 2020 20:55

    Content type: Research article

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  4. Gene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is clea...

    Authors: Nicholas M. Thomson, Chuanzhen Zhang, Eleftheria Trampari and Mark J. Pallen

    Citation: BMC Biotechnology 2020 20:54

    Content type: Research article

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  5. Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific ...

    Authors: Sandra Klein, Daniel Stern and Frank Seeber

    Citation: BMC Biotechnology 2020 20:53

    Content type: Methodology article

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  6. Obesity and its related diseases are increasing worldwide. One of the best therapeutic strategies for obesity management is through the inhibition of pancreatic lipase (PL) enzyme. So far orlistat is the only ...

    Authors: Sarah Mohamed El-Korany, Omneya Mohamed Helmy, Ali Mahmoud El-Halawany, Yasser El-Mohammadi Ragab and Hamdallah Hafez Zedan

    Citation: BMC Biotechnology 2020 20:52

    Content type: Research article

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  7. Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosa...

    Authors: Amitchihoué Franck Sessou, Jane W. Kahia, Jerome Anani Houngue, Elijah Miinda Ateka, Colombe Dadjo and Corneille Ahanhanzo

    Citation: BMC Biotechnology 2020 20:51

    Content type: Research article

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  8. Pelargonium sidoides is an important traditional medicine in South Africa with a well-defined history of both traditional and documented use of an aqueous-ethanolic formulation of the roots of P. sidoides (EPs 76...

    Authors: V. Lukman, S. W. Odeyemi, R. L. Roth, L. Mbabala, N. Tshililo, N. M. Vlok, M. J. B. Dewar and C. P. Kenyon

    Citation: BMC Biotechnology 2020 20:50

    Content type: Research article

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  9. Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media ...

    Authors: Yijiang Huang, Daniel Seitz, Yan Chevalier, Peter E. Müller, Volkmar Jansson and Roland M. Klar

    Citation: BMC Biotechnology 2020 20:48

    Content type: Research article

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  10. Approximately 40% of prescribed drugs exert their activity via GTP-binding protein-coupled receptors (GPCRs). Once activated, these receptors cause transient changes in the concentration of second messengers, ...

    Authors: Nadine Gruteser, Viktoria Kohlhas, Sabine Balfanz, Arne Franzen, Anne Günther, Andreas Offenhäusser, Frank Müller, Viacheslav Nikolaev, Martin J. Lohse and Arnd Baumann

    Citation: BMC Biotechnology 2020 20:47

    Content type: Methodology article

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  11. Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) us...

    Authors: Young-Hoon Jeong, Ukjin Kim, Seul-Gi Lee, Bokyeong Ryu, Jin Kim, Artyuhov Igor, Jong Soo Kim, Cho-Rok Jung, Jae-Hak Park and C-Yoon Kim

    Citation: BMC Biotechnology 2020 20:45

    Content type: Research article

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  12. Oral administration is the most common way to deliver drugs to the systemic circulation or target organs. Orally administered drugs are absorbed in the intestine and metabolized in the intestine and liver. In ...

    Authors: Yumi Ohta, Kanako Kazuki, Satoshi Abe, Mitsuo Oshimura, Kaoru Kobayashi and Yasuhiro Kazuki

    Citation: BMC Biotechnology 2020 20:44

    Content type: Research article

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  13. Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit devel...

    Authors: Kasturi Dasgupta, Sara Hotton, William Belknap, Yasra Syed, Christopher Dardick, Roger Thilmony and James G. Thomson

    Citation: BMC Biotechnology 2020 20:43

    Content type: Research article

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  14. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang

    Citation: BMC Biotechnology 2020 20:42

    Content type: Correction

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    The original article was published in BMC Biotechnology 2019 19:9

  15. The enzyme-linked immunosorbent assay (ELISA), is the most widely used and reliable clinical routine method for the detection of important protein markers in healthcare. Improving ELISAs is crucial for detecti...

    Authors: Tania García-Maceira, Fé I. García-Maceira, José A. González-Reyes and Elier Paz-Rojas

    Citation: BMC Biotechnology 2020 20:41

    Content type: Methodology article

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  16. Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galacto...

    Authors: Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi

    Citation: BMC Biotechnology 2020 20:40

    Content type: Research article

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  17. With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective ...

    Authors: Margaret T. Ho, Michelle S. M. Li, Tim McDowell, Jacqueline MacDonald and Ze-Chun Yuan

    Citation: BMC Biotechnology 2020 20:39

    Content type: Research article

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  18. Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract (GIT) and to date, no efficient treatments exist. Interleukin-10 (IL-10), one of the most ...

    Authors: Meritxell Zurita-Turk, Bianca Mendes Souza, Camila Prósperi de Castro, Vanessa Bastos Pereira, Vanessa Pecini da Cunha, Tatiane Melo Preisser, Ana Maria Caetano de Faria, Denise Carmona Cara Machado and Anderson Miyoshi

    Citation: BMC Biotechnology 2020 20:38

    Content type: Research article

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  19. Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts a...

    Authors: Hui Cai, Gefei Chen, Hairui Yu, Ying Tang, Sidong Xiong and Xingmei Qi

    Citation: BMC Biotechnology 2020 20:37

    Content type: Research article

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  20. Esterases are widely distributed in nature and have important applications in medical, industrial and physiological. Recently, the increased demand for flavor esters has prompted the search of catalysts like l...

    Authors: Xianghai Cai, Lin Lin, Yaling Shen, Wei Wei and Dong-zhi Wei

    Citation: BMC Biotechnology 2020 20:36

    Content type: Research article

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  21. Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by val...

    Authors: Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet and Ross D. Houston

    Citation: BMC Biotechnology 2020 20:35

    Content type: Methodology article

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  22. This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-tim...

    Authors: Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, Mariana Ahamad, Maizan Mohamed, Chan Yean Yean and Boon Huat Lim

    Citation: BMC Biotechnology 2020 20:34

    Content type: Research article

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  23. Helicobacter pylori (H. pylori) infection is a serious human health threat. The empiric H. pylori treatment paradigm guided by traditional testing technologies has led to antibiotic resistance. Here, we improved ...

    Authors: Ling Deng, Xiao-Yi He, Bin Tang, Yang Xiang and Juan-Juan Yue

    Citation: BMC Biotechnology 2020 20:33

    Content type: Methodology article

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  24. Oncolytic viruses are playing an increasingly important role in cancer immunotherapy applications. Given the preclinical and clinical efficacy of these virus-based therapeutics, there is a need for fast, simpl...

    Authors: Lisa A. Santry, Renaud Jacquemart, Melissa Vandersluis, Mochao Zhao, Jake M. Domm, Thomas M. McAusland, Xiaojiao Shang, Pierre M. Major, James G. Stout and Sarah K. Wootton

    Citation: BMC Biotechnology 2020 20:32

    Content type: Methodology article

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  25. Immunotoxin is a hybrid protein consisting of a toxin moiety that is linked to a targeting moiety for the purpose of specific elimination of target cells. Toxins used in traditional immunotoxins are practicall...

    Authors: William F. Jiemy, Lih Fhung Hiew, Hong Xi Sha, Lionel L. A. In and Jung Shan Hwang

    Citation: BMC Biotechnology 2020 20:31

    Content type: Research article

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  26. Treatment of cancers has largely benefited from the development of immunotherapy. In particular, Chimeric Antigen Receptor (CAR) redirected T cells have demonstrated impressive efficacy against B-cell malignan...

    Authors: Pierre Dillard, Maren Lie, Elizabeth Baken, Viola Hélène Lobert, Emmanuelle Benard, Hakan Köksal, Else Marit Inderberg and Sébastien Wälchli

    Citation: BMC Biotechnology 2020 20:30

    Content type: Methodology article

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  27. The bacterial genus Exiguobacterium includes several species that inhabit environments with a wide range of temperature, salinity, and pH. This is why the microorganisms from this genus are known generically as p...

    Authors: Javier Orizola, Mirtha Ríos-Silva, Claudia Muñoz-Villagrán, Esteban Vargas, Claudio Vásquez and Felipe Arenas

    Citation: BMC Biotechnology 2020 20:29

    Content type: Research article

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  28. Legionella pneumophila (L.pneumophila), a Gram-negative small microorganism, causes hospital-acquired pneumonia especially in immunocompromised patients. Vaccination may be an effective method for preventing L.pn...

    Authors: Yingying Chen, Zehui Yang, Ying Dong and Yu Chen

    Citation: BMC Biotechnology 2020 20:28

    Content type: Research article

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  29. Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxido...

    Authors: Wissal Ben Ali, Delphine Chaduli, David Navarro, Christian Lechat, Annick Turbé-Doan, Emmanuel Bertrand, Craig B. Faulds, Giuliano Sciara, Laurence Lesage-Meessen, Eric Record and Tahar Mechichi

    Citation: BMC Biotechnology 2020 20:27

    Content type: Research article

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  30. Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control...

    Authors: Jana Neuhold, Katharina Radakovics, Anita Lehner, Florian Weissmann, Maria Queralt Garcia, Mari Carmen Romero, Nicholas S. Berrow and Peggy Stolt-Bergner

    Citation: BMC Biotechnology 2020 20:26

    Content type: Methodology article

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  31. Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection...

    Authors: Zsófia Bánfalvi, Edina Csákvári, Vanda Villányi and Mihály Kondrák

    Citation: BMC Biotechnology 2020 20:25

    Content type: Research article

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  32. Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, howev...

    Authors: Agnieszka Gawin, Helga Ertesvåg, Sine Alise Hartvigsen Hansen, Jostein Malmo and Trygve Brautaset

    Citation: BMC Biotechnology 2020 20:24

    Content type: Research article

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  33. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu

    Citation: BMC Biotechnology 2020 20:23

    Content type: Retraction Note

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    The original article was published in BMC Biotechnology 2018 18:7

  34. In Norway, 3 million discarded egg-laying hens are destructed annually, which equals 1500 tons pure hen meat. Due to the slaughter methods used, this raw material is handled as a high-risk waste, while in real...

    Authors: Veronica Hjellnes, Rasa Šližyte, Turid Rustad, Ana Karina Carvajal and Kirsti Greiff

    Citation: BMC Biotechnology 2020 20:22

    Content type: Research article

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  35. This study investigated the feasibility of enhancing anaerobic digestion of sewage sludge with triple, dual, and individual pretreatment of waste activated sludge with heat, alkalinity, and hydrogen peroxide. ...

    Authors: Salar Siami, Behnoush Aminzadeh, Razieh Karimi and Seyed Mostafa Hallaji

    Citation: BMC Biotechnology 2020 20:21

    Content type: Research article

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  36. The coexistence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals has deleterious effects on environmental quality. Few reports have studied the mechanisms of plant inoculation with Piriformospora indic...

    Authors: Liang Li, Pengyue Zhu, Xiaoyang Wang and Zhenhua Zhang

    Citation: BMC Biotechnology 2020 20:20

    Content type: Research article

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  37. Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of ...

    Authors: Abbas Tanhaeian, Mehdi Mirzaii, Zana Pirkhezranian and Mohammad Hadi Sekhavati

    Citation: BMC Biotechnology 2020 20:19

    Content type: Research article

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  38. Contagious ecthyma or Orf is known as a zoonotic disease remains prevalently worldwide despite the application of some control strategies against it. RNAi particularly shRNA provides us with the chance to tack...

    Authors: Leila Asadi Samani, Behnaz Saffar, Azam Mokhtari and Ehsan Arefian

    Citation: BMC Biotechnology 2020 20:18

    Content type: Research article

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  39. Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially av...

    Authors: Szilvia Krisztina Nagy, Brigitta Margit Kállai, Judit András and Tamás Mészáros

    Citation: BMC Biotechnology 2020 20:17

    Content type: Methodology article

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  40. The type I human interferon (IFN) family consists of a group of cytokines with a multiplicity of biological activities, including antiviral, antitumor, and immunomodulatory effects. However, because the half-l...

    Authors: Qian Zhang, Chao Wang, Fenlian Ma, Lihong Yao, Hanchun Gao, Luyan Zhu and Lishu Zheng

    Citation: BMC Biotechnology 2020 20:16

    Content type: Research article

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  41. Infections caused by fungi are often refractory to conventional therapies and urgently require the development of novel options, such as immunotherapy. To produce therapeutic antibodies, a plant-based expressi...

    Authors: Marcello Catellani, Chiara Lico, Mauro Cerasi, Silvia Massa, Carla Bromuro, Antonella Torosantucci, Eugenio Benvenuto and Cristina Capodicasa

    Citation: BMC Biotechnology 2020 20:15

    Content type: Research article

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  42. Intercropping, an essential cultivation pattern in modern agricultural systems, increases crop yields and soil quality. Cassava and peanut intercropping systems exhibit advantages in solar utilization and cadm...

    Authors: Xiumei Tang, Ruichun Zhong, Jing Jiang, Liangqiong He, Zhipeng Huang, Guoying Shi, Haining Wu, Jing Liu, Faqian Xiong, Zhuqiang Han, Ronghua Tang and Longfei He

    Citation: BMC Biotechnology 2020 20:13

    Content type: Research article

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  43. Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant bioma...

    Authors: Leigh Gebbie, Tuan Tu Dam, Rebecca Ainscough, Robin Palfreyman, Li Cao, Mark Harrison, Ian O’Hara and Robert Speight

    Citation: BMC Biotechnology 2020 20:12

    Content type: Research article

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  44. As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in ...

    Authors: Jean-Michel Michno, Kamaldeep Virdi, Adrian O. Stec, Junqi Liu, Xiaobo Wang, Yer Xiong and Robert M. Stupar

    Citation: BMC Biotechnology 2020 20:10

    Content type: Research article

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  45. Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a...

    Authors: Bo Wang, Meifang Yu, Xianglin Zhu and Li Zhu

    Citation: BMC Biotechnology 2020 20:9

    Content type: Methodology article

    Published on:

  46. The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternati...

    Authors: Tao He, Jörg Hausdorf, Yan Chevalier and Roland M. Klar

    Citation: BMC Biotechnology 2020 20:8

    Content type: Research article

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  47. Clostridium perfringens is the causative agent of several diseases and enteric infections in animals and humans. The virulence of C. perfringens is largely attributable to the production of numerous toxins; of th...

    Authors: Katia Forti, Monica Cagiola, Martina Pellegrini, Lucia Anzalone, Antonella Di Paolo, Sara Corneli, Giulio Severi and Antonio De Giuseppe

    Citation: BMC Biotechnology 2020 20:7

    Content type: Research article

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