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  1. Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ...

    Authors: Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans and Anne-Catherine Servais

    Citation: BMC Biotechnology 2019 19:81

    Content type: Research article

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  2. Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell...

    Authors: Yong Xu Mu, Yu Xia Zhao, Bing Yao Li, Hong Jing Bao, Hui Jiang, Xiao Lei Qi, Li Yun Bai, Yun Hong Wang, Zhi Jie Ma and Xiao Yun Wu

    Citation: BMC Biotechnology 2019 19:80

    Content type: Methodology article

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  3. Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p...

    Authors: Elinea O. Freitas, Bruno P. Melo, Isabela T. Lourenço-Tessutti, Fabrício B. M. Arraes, Regina M. Amorim, Maria E. Lisei-de-Sá, Julia A. Costa, Ana G. B. Leite, Muhammad Faheem, Márcio A. Ferreira, Carolina V. Morgante, Elizabeth P. B. Fontes and Maria F. Grossi-de-Sa

    Citation: BMC Biotechnology 2019 19:79

    Content type: Research article

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  4. Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. H...

    Authors: Yingshuo Hou, Siyu Chen, Jianjun Wang, Guizhen Liu, Sheng Wu and Yong Tao

    Citation: BMC Biotechnology 2019 19:76

    Content type: Research article

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  5. DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transge...

    Authors: Kristian Alsbjerg Skipper, Anne Kruse Hollensen, Michael N. Antoniou and Jacob Giehm Mikkelsen

    Citation: BMC Biotechnology 2019 19:75

    Content type: Research article

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  6. Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated...

    Authors: Guoxing Zheng, Qingqing Zhu, Junchao Dong, Xin Lin and Chengming Zhu

    Citation: BMC Biotechnology 2019 19:74

    Content type: Research article

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  7. Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assa...

    Authors: Aurélie Bak and Joanne B. Emerson

    Citation: BMC Biotechnology 2019 19:73

    Content type: Methodology article

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  8. In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the me...

    Authors: Lei Yang, Tore Linde, Abeer H. Hossain, Mette Lübeck, Peter J. Punt and Peter S. Lübeck

    Citation: BMC Biotechnology 2019 19:72

    Content type: Research article

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  9. The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity...

    Authors: Taj Arndell, Niharika Sharma, Peter Langridge, Ute Baumann, Nathan S. Watson-Haigh and Ryan Whitford

    Citation: BMC Biotechnology 2019 19:71

    Content type: Research article

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  10. Aspergillus oryzae, a useful industrial filamentous fungus, produces limited varieties of secondary metabolites, such as kojic acid. Thus, for the production of valuable secondary metabolites by genetic engineeri...

    Authors: Koichi Tamano, Mahoko Kuninaga, Naoshi Kojima, Myco Umemura, Masayuki Machida and Hideaki Koike

    Citation: BMC Biotechnology 2019 19:70

    Content type: Research article

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  11. Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the resear...

    Authors: Prassan Choudhary, Sudipta Das, Hillol Chakdar, Arjun Singh, Sanjay Kumar Goswami and Anil Kumar Saxena

    Citation: BMC Biotechnology 2019 19:69

    Content type: Methodology article

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  12. Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the ze...

    Authors: David Simon Kleinhans and Virginie Lecaudey

    Citation: BMC Biotechnology 2019 19:68

    Content type: Methodology article

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  13. Lymphocyte-activation gene (LAG)3 is a 498 aa transmembrane type I protein acting as an immune inhibitory receptor. It is expressed on activated lymphocytes, natural killer cells and plasmacytoid dendritic cel...

    Authors: Alessandro Ascione, Claudia Arenaccio, Alessandra Mallano, Michela Flego, Mara Gellini, Mauro Andreotti, Craig Fenwick, Giuseppe Pantaleo, Stefano Vella and Maurizio Federico

    Citation: BMC Biotechnology 2019 19:67

    Content type: Research article

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  14. Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybe...

    Authors: Di Qin, Xiao-Yi Liu, Cristina Miceli, Qi Zhang and Pi-wu Wang

    Citation: BMC Biotechnology 2019 19:66

    Content type: Research article

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  15. Ebola hemorrhagic fever is caused by the Ebola filovirus (EBOV), which is one of the most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subv...

    Authors: Michela Flego, Aldo Frau, Luisa Accardi, Alessandra Mallano, Alessandro Ascione, Mara Gellini, Elisa Fanunza, Stefano Vella, Paola Di Bonito and Enzo Tramontano

    Citation: BMC Biotechnology 2019 19:64

    Content type: Research article

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  16. In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to...

    Authors: Jian Pang, Junshu Wang, Zhanying Liu, Qiancheng Zhang and Qingsheng Qi

    Citation: BMC Biotechnology 2019 19:63

    Content type: Research article

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  17. In personalized medicine, companion diagnostic tests provide additional information to help select a treatment option likely to be optimal for a patient. Although such tests include several techniques for dete...

    Authors: Jr-Kai Huang, Ling Fan, Tao-Yeuan Wang and Pao-Shu Wu

    Citation: BMC Biotechnology 2019 19:62

    Content type: Methodology article

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    The Correction to this article has been published in BMC Biotechnology 2019 19:77

  18. Laccases are multicopper oxidases, which are assigned into auxiliary activity family 1 (AA1) in the CAZy database. These enzymes, catalyzing the oxidation of phenolic and nonphenolic substrates coupled to redu...

    Authors: Majid Haddad Momeni, Paolo Bollella, Roberto Ortiz, Esben Thormann, Lo Gorton and Maher Abou Hachem

    Citation: BMC Biotechnology 2019 19:61

    Content type: Research article

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  19. To directly assess the biological role of oligosaccharides in recombinant equine chorionic gonadotropin (rec-eCG) functioning, cDNA encoding the full-length eCGβ-subunit was fused with the mature protein part ...

    Authors: Kwan-Sik Min, Jong-Ju Park, Munkhzaya Byambaragchaa and Myung-Hwa Kang

    Citation: BMC Biotechnology 2019 19:60

    Content type: Research article

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  20. Heparinase I from Pedobacter heparinus (Ph-HepI), which specifically cleaves heparin and heparan sulfate, is one of the most extensively studied glycosaminoglycan lyases. Enzymatic degradation of heparin by hepar...

    Authors: Chuan Zhang, Bao-Cheng Yang, Wen-Ting Liu, Zhong-Yuan Li, Ya-Jian Song, Tong-Cun Zhang and Xue-Gang Luo

    Citation: BMC Biotechnology 2019 19:59

    Content type: Research article

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  21. Efficient xylose fermentation still demands knowledge regarding xylose catabolism. In this study, metabolic flux analysis (MFA) and metabolomics were used to improve our understanding of xylose metabolism. Thu...

    Authors: Henrique C. T. Veras, Christiane G. Campos, Igor F. Nascimento, Patrícia V. Abdelnur, João R. M. Almeida and Nádia S. Parachin

    Citation: BMC Biotechnology 2019 19:58

    Content type: Research article

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  22. The textural characteristics of fermented dairy products are important quality parameters that play a major role in their stability and consumer’s acceptance. The aim of this study was to investigate the influ...

    Authors: Soumaya El Bouchikhi, Philippe Pagès, Yassir El Alaoui, Azeddine Ibrahimi and Yahya Bensouda

    Citation: BMC Biotechnology 2019 19:57

    Content type: Research article

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  23. Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main ad...

    Authors: Thomas Thisted, Zuzana Biesova, Celine Walmacq, Everett Stone, Max Rodnick-Smith, Shaheda S. Ahmed, Stephen K. Horrigan, Bo Van Engelen, Charles Reed and Matthew W. Kalnik

    Citation: BMC Biotechnology 2019 19:56

    Content type: Research article

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  24. The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme a...

    Authors: Xiaoping Song, Changsheng Shao, Yugang Guo, Yajie Wang and Jingjing Cai

    Citation: BMC Biotechnology 2019 19:54

    Content type: Research article

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  25. Increasing CO2 emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of mari...

    Authors: Aiyou Huang, Songcui Wu, Wenhui Gu, Yuanxiang Li, Xiujun Xie and Guangce Wang

    Citation: BMC Biotechnology 2019 19:53

    Content type: Research article

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  26. Packing materials is a critical design consideration when employing biological reactor to treat malodorous gases. The acidification of packing bed usually results in a significant drop in the removal efficienc...

    Authors: Xiang Tu, Meiying Xu, Jianjun Li, Enze Li, Rongfang Feng, Gang Zhao, Shaobin Huang and Jun Guo

    Citation: BMC Biotechnology 2019 19:52

    Content type: Research article

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  27. A mesophilic xylanase PjxA from Penicillium janthinellum MA21601 has high specific activity under acidic condition and holds great potential for applications in the animal feed industry. To enhance the thermostab...

    Authors: Ke Xiong, Jie Hou, Yuefeng Jiang, Xiuting Li, Chao Teng, Qin Li, Guangsen Fan, Ran Yang and Chengnan Zhang

    Citation: BMC Biotechnology 2019 19:51

    Content type: Research article

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  28. Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length ...

    Authors: Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman and Biju Parekkadan

    Citation: BMC Biotechnology 2019 19:50

    Content type: Methodology article

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  29. S-Phenyl-L-cysteine is regarded as having potential applicability as an antiretroviral/protease inhibitor for human immunodeficiency virus (HIV). In the present study, optically active S-phenyl-L-cysteine was pre...

    Authors: Lisheng Xu, Xingtao Zhang, Guizhen Gao and Sun Yue

    Citation: BMC Biotechnology 2019 19:49

    Content type: Research article

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  30. Engineered vaccine proteins incorporating both antigen and adjuvant components are constructed with the aim of combining functions to induce effective protective immunity. Bacterial flagellin is a strong candi...

    Authors: Casey M. Gries, Rohith R. Mohan, Dimitrios Morikis and David D. Lo

    Citation: BMC Biotechnology 2019 19:48

    Content type: Research article

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  31. Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study wa...

    Authors: Yang Gao, Feng Feng Sang, De Lan Meng, Yi Wang, Wen Tao Ma and De Kun Chen

    Citation: BMC Biotechnology 2019 19:47

    Content type: Research article

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  32. Natamycin is an antifungal polyene macrolide antibiotic with wide applications in health and food industries. Currently, it is the only antifungal food additive with the GRAS status (Generally Regarded as Safe).

    Authors: Elsayed Ahmed Elsayed, Mohamed A. Farid and Hesham A. El-Enshasy

    Citation: BMC Biotechnology 2019 19:46

    Content type: Research article

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  33. Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environ...

    Authors: Ge Zhang, Shugui Li, Yingbo Xu, Juan Wang, Fan Wang, Yuhua Xin, Zhong Shen, Haibo Zhang, Ming Ma and Haobao Liu

    Citation: BMC Biotechnology 2019 19:45

    Content type: Research article

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  34. Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR)...

    Authors: Alexandre Juillerat, Diane Tkach, Brian W. Busser, Sonal Temburni, Julien Valton, Aymeric Duclert, Laurent Poirot, Stéphane Depil and Philippe Duchateau

    Citation: BMC Biotechnology 2019 19:44

    Content type: Research article

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  35. Proteases are hydrolytic enzymes that catalyze peptide linkage cleavage reactions at the level of proteins and peptides with different degrees of specificity. This group draws the attention of industry. More t...

    Authors: Maroua Omrane Benmrad, Sondes Mechri, Nadia Zaraî Jaouadi, Mouna Ben Elhoul, Hatem Rekik, Sami Sayadi, Samir Bejar, Nabil Kechaou and Bassem Jaouadi

    Citation: BMC Biotechnology 2019 19:43

    Content type: Research article

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  36. Artificial Mitochondrial Transfer or Transplant (AMT/T) can be used to reduce the stress and loss of viability of damaged cells. In MitoCeption, a type of AMT/T, the isolated mitochondria and recipient cells a...

    Authors: Francisco Cabrera, Mayra Ortega, Francesca Velarde, Eliseo Parra, Stephany Gallardo, Diego Barba, Lina Soto, Gabriela Peña, Luis Alberto Pedroza, Christian Jorgensen, Maroun Khoury and Andrés Caicedo

    Citation: BMC Biotechnology 2019 19:42

    Content type: Research article

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  37. Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very compe...

    Authors: Bruna S. Fernandes, Oscar Dias, Gisela Costa, Antonio A. Kaupert Neto, Tiago F. C. Resende, Juliana V. C. Oliveira, Diego M. Riaño-Pachón, Marcelo Zaiat, José G. C. Pradella and Isabel Rocha

    Citation: BMC Biotechnology 2019 19:41

    Content type: Research article

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  38. Natural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current of...

    Authors: Laurence O. W. Wilson, Sara Hetzel, Christopher Pockrandt, Knut Reinert and Denis C. Bauer

    Citation: BMC Biotechnology 2019 19:40

    Content type: Software

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  39. Aerobic side chain degradation of phytosterols by actinobacteria is the basis for the industrial production of androstane steroids which are the starting materials for the synthesis of steroid hormones. A nati...

    Authors: Eugeny Y. Bragin, Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Dmitry V. Dovbnya and Marina V. Donova

    Citation: BMC Biotechnology 2019 19:39

    Content type: Research article

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  40. Anti-Tumor Necrosis Factor-alpha therapy has become clinically important for treating inflammatory bowel disease. However, the use of conventional immunotherapy requires a systemic exposure of patients and col...

    Authors: Maria José Chiabai, Juliana Franco Almeida, Mariana Gabriela Dantas de Azevedo, Suelen Soares Fernandes, Vanessa Bastos Pereira, Raffael Júnio Araújo de Castro, Márcio Sousa Jerônimo, Isabel Garcia Sousa, Leonora Maciel de Souza Vianna, Anderson Miyoshi, Anamelia Lorenzetti Bocca, Andrea Queiroz Maranhão and Marcelo Macedo Brigido

    Citation: BMC Biotechnology 2019 19:38

    Content type: Research article

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  41. CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly cont...

    Authors: Bent Larsen Petersen, Svenning Rune Möller, Jozef Mravec, Bodil Jørgensen, Mikkel Christensen, Ying Liu, Hans H. Wandall, Eric Paul Bennett and Zhang Yang

    Citation: BMC Biotechnology 2019 19:36

    Content type: Research article

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  42. Viral vectors are invaluable tools to transfer genes and/or regulatory sequences into differentiated cells such as pancreatic cells. To date, several kinds of viral vectors have been used to transduce differen...

    Authors: Olivier Albagli, Alicia Maugein, Lukas Huijbregts, Delphine Bredel, Géraldine Carlier, Patrick Martin and Raphaël Scharfmann

    Citation: BMC Biotechnology 2019 19:35

    Content type: Research article

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  43. In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more tha...

    Authors: Sin-Yeang Teow, Kitson Liew, Mohd Firdaus Che Mat, Marini Marzuki, Norazlin Abdul Aziz, Tai-Lin Chu, Munirah Ahmad and Alan Soo-Beng Khoo

    Citation: BMC Biotechnology 2019 19:34

    Content type: Methodology article

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  44. Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify mo...

    Authors: Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark and Margaret C. M. Smith

    Citation: BMC Biotechnology 2019 19:32

    Content type: Research article

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  45. Copy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders. Here we investigate a CNV on chromosome 10q11.22 that spans NPY4R, the gene ...

    Authors: Kateryna Shebanits, Torsten Günther, Anna C. V. Johansson, Khurram Maqbool, Lars Feuk, Mattias Jakobsson and Dan Larhammar

    Citation: BMC Biotechnology 2019 19:31

    Content type: Research article

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