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  1. Assays detecting human antigen-specific antibodies are medically useful. However, the usefulness of existing simple immunoassay formats is limited by technical considerations such as sera antibodies to contami...

    Authors: Peter D Burbelo, Radoslav Goldman and Thomas L Mattson
    Citation: BMC Biotechnology 2005 5:22
    Content type: Methodology article Published on:

  2. Human Artificial Chromosomes (HACs) are potentially useful vectors for gene transfer studies and for functional annotation of the genome because of their suitability for cloning, manipulating and transferring ...

    Authors: Joydeep Basu, George Compitello, Gregory Stromberg, Huntington F Willard and Gil Van Bokkelen
    Citation: BMC Biotechnology 2005 5:21
    Content type: Research article Published on:

  3. DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widesp...

    Authors: Jonathan Z Long, Chantal S Lackan and Anna-Katerina Hadjantonakis
    Citation: BMC Biotechnology 2005 5:20
    Content type: Methodology article Published on:

  4. The expression of recombinant proteins in eukaryotic cells requires the fusion of the coding region to a promoter functional in the eukaryotic cell line. Viral promoters are very often used for this purpose. T...

    Authors: Astrid Lewin, Martin Mayer, Janet Chusainow, Daniela Jacob and Bernd Appel
    Citation: BMC Biotechnology 2005 5:19
    Content type: Research article Published on:

  5. Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contras...

    Authors: Victoria Yamazaki, Oksana Sirenko, Robert J Schafer, Luat Nguyen, Thomas Gutsmann, Lore Brade and Jay T Groves
    Citation: BMC Biotechnology 2005 5:18
    Content type: Methodology article Published on:

  6. The hCMV promoter is very commonly used for high level expression of transgenes in mammalian cells, but its utility is hindered by transcriptional silencing. Large genomic fragments incorporating the CpG island r...

    Authors: Steven Williams, Tracey Mustoe, Tony Mulcahy, Mark Griffiths, David Simpson, Michael Antoniou, Alistair Irvine, Andrew Mountain and Robert Crombie
    Citation: BMC Biotechnology 2005 5:17
    Content type: Research article Published on:

  7. High-throughput screening is used by the pharmaceutical industry for identifying lead compounds that interact with targets of pharmacological interest. Because of the key role that aberrant regulation of prote...

    Authors: Frauke Rininsland, Casey Stankewicz, Wendy Weatherford and Duncan McBranch
    Citation: BMC Biotechnology 2005 5:16
    Content type: Methodology article Published on:

  8. The wide variety of real-time amplification platforms currently available has determined that standardisation of DNA measurements is a fundamental aspect involved in the comparability of results.

    Authors: Carol E Donald, Fizza Qureshi, Malcolm J Burns, Marcia J Holden, Joseph R Blasic Jr and Alison J Woolford
    Citation: BMC Biotechnology 2005 5:15
    Content type: Research article Published on:

  9. The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types ...

    Authors: Erik Sahai, Jeffrey Wyckoff, Ulrike Philippar, Jeffrey E Segall, Frank Gertler and John Condeelis
    Citation: BMC Biotechnology 2005 5:14
    Content type: Methodology article Published on:

  10. The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-b...

    Authors: Luke S Lambeth, Robert J Moore, Morley Muralitharan, Brian P Dalrymple, Sean McWilliam and Timothy J Doran
    Citation: BMC Biotechnology 2005 5:13
    Content type: Research article Published on:

  11. Analyzing apoptosis has been an integral component of many biological studies. However, currently available methods for quantifying apoptosis have various limitations including multiple, sometimes cell-damagin...

    Authors: Deborah Ribble, Nathaniel B Goldstein, David A Norris and Yiqun G Shellman
    Citation: BMC Biotechnology 2005 5:12
    Content type: Methodology article Published on:

  12. Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a signi...

    Authors: Tristan Ruysschaert, Audrey Marque, Jean-Luc Duteyrat, Sylviane Lesieur, Mathias Winterhalter and Didier Fournier
    Citation: BMC Biotechnology 2005 5:11
    Content type: Methodology article Published on:

  13. Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.

    Authors: Frédéric R Raymond, Hoang-Anh Ho, Régis Peytavi, Luc Bissonnette, Maurice Boissinot, François J Picard, Mario Leclerc and Michel G Bergeron
    Citation: BMC Biotechnology 2005 5:10
    Content type: Methodology article Published on:

  14. Uromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned ...

    Authors: Yue-Jin Huang, Nathalie Chretien, Annie S Bilodeau, Jiang Feng Zhou, Anthoula Lazaris and Costas N Karatzas
    Citation: BMC Biotechnology 2005 5:9
    Content type: Research article Published on:

  15. We have developed an oligonucleotide microarray (genosensor) utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene. Isola...

    Authors: Angélica Rangel-López, Rogelio Maldonado-Rodríguez, Mauricio Salcedo-Vargas, Juana Mercedes Espinosa-Lara, Alfonso Méndez-Tenorio and Kenneth L Beattie
    Citation: BMC Biotechnology 2005 5:8
    Content type: Methodology article Published on:

  16. The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic s...

    Authors: Sreelatha Meleth, Jessy Deshane and Helen Kim
    Citation: BMC Biotechnology 2005 5:7
    Content type: Research article Published on:

  17. In many eukaryotic cells, double-stranded RNA (dsRNA) triggers RNA interference (RNAi), the specific degradation of RNA of homologous sequence. RNAi is now a major tool for reverse-genetics projects, including...

    Authors: Filippo Rusconi, Mickaël Durand-Dubief and Philippe Bastin
    Citation: BMC Biotechnology 2005 5:6
    Content type: Methodology article Published on:

  18. Transcriptional profiling using microarrays has developed into a key molecular tool for the elucidation of gene function and gene regulation. Microarray platforms based on either oligonucleotides or purified a...

    Authors: Valtteri Wirta, Anders Holmberg, Morten Lukacs, Peter Nilsson, Pierre Hilson, Mathias Uhlén, Rishikesh P Bhalerao and Joakim Lundeberg
    Citation: BMC Biotechnology 2005 5:5
    Content type: Methodology article Published on:

  19. Common oral diseases and dental caries can be prevented effectively by passive immunization. In humans, passive immunotherapy may require the use of humanized or human antibodies to prevent adverse immune resp...

    Authors: Michael B Kupper, Michael Huhn, Holger Spiegel, Julian KC Ma, Stefan Barth, Rainer Fischer and Ricarda Finnern
    Citation: BMC Biotechnology 2005 5:4
    Content type: Research article Published on:

  20. In the past ten years there has been a growing interest in engineering Gram-positive bacteria for biotechnological applications, including vaccine delivery and production of recombinant proteins. Usually, bact...

    Authors: Roberta Provvedi, Tiziana Maggi, Marco R Oggioni, Riccardo Manganelli and Gianni Pozzi
    Citation: BMC Biotechnology 2005 5:3
    Content type: Methodology article Published on:

  21. Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is part...

    Authors: Cristina Hartshorn, Aleksandra Anshelevich and Lawrence J Wangh
    Citation: BMC Biotechnology 2005 5:2
    Content type: Methodology article Published on:

  22. Recombinant adenoviral vectors are highly efficient for in vitro and in vivo gene delivery. They can easily be produced in large numbers, transduce a wide variety of cell types and generate high levels of tran...

    Authors: Dalibor Antolovic, Moritz Koch, Inga Bohlmann, Peter Kienle, Markus Büchler and Jürgen Weitz
    Citation: BMC Biotechnology 2005 5:1
    Content type: Methodology article Published on:

  24. In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied i...

    Authors: Michael R Dyson, S Paul Shadbolt, Karen J Vincent, Rajika L Perera and John McCafferty
    Citation: BMC Biotechnology 2004 4:32
    Content type: Research article Published on:

  25. Modification of leaf traits in sugar beet requires a strong leaf specific promoter. With such a promoter, expression in taproots can be avoided which may otherwise take away available energy resources for suga...

    Authors: Dietmar J Stahl, Dorothee U Kloos and Reinhard Hehl
    Citation: BMC Biotechnology 2004 4:31
    Content type: Research article Published on:

  26. Laser microdissection allows precise isolation of specific cell types and compartments from complex tissues. To analyse proteins from small cell numbers, we combine laser-microdissection and manipulation (LMM)...

    Authors: Grazyna Kwapiszewska, Markus Meyer, Ralf Bogumil, Rainer M Bohle, Werner Seeger, Norbert Weissmann and Ludger Fink
    Citation: BMC Biotechnology 2004 4:30
    Content type: Methodology article Published on:

  27. The blasticidin S resistance gene (bsr) is a selectable marker used for gene transfer experiments. The bsr gene encodes for blasticidin S (BS) deaminase, which has a specific activity upon BS. Therefore, its expr...

    Authors: Fernanda Mara Bento, Daniela Takeshita, Chester Bittencourt Sacramento, Tamara Rocha Machado, Monica Beatriz Mathor, Adriana Karaoglanovic Carmona and Sang Won Han
    Citation: BMC Biotechnology 2004 4:29
    Content type: Research article Published on:

  28. Fast, cheap and reliable methods are needed to identify large populations, which may be at risk in relation to environmental exposure. Polymorphisms in NAT1 (N-acetyl transferase) may be suitable markers to id...

    Authors: Pavel Soucek, Camilla Furu Skjelbred, Marit Svendsen, Tom Kristensen, Elin H Kure and Vessela N Kristensen
    Citation: BMC Biotechnology 2004 4:28
    Content type: Methodology article Published on:

  29. Mucosal delivery of therapeutic protein drugs or vaccines is actively investigated, in order to improve bioavailability and avoid side effects associated with systemic administration. Orally administered bacte...

    Authors: Stefano Porzio, Paola Bossù, Paolo Ruggiero, Diana Boraschi and Aldo Tagliabue
    Citation: BMC Biotechnology 2004 4:27
    Content type: Research article Published on:

  32. Each of the human genes or transcriptional units is likely to contain single nucleotide polymorphisms that may give rise to sequence variation between individuals and tissues on the level of RNA. Based on rece...

    Authors: Ulrika Liljedahl, Mona Fredriksson, Andreas Dahlgren and Ann-Christine Syvänen
    Citation: BMC Biotechnology 2004 4:24
    Content type: Methodology article Published on:

  33. Several cell lines and primary cultures benefit from the use of positively charged extracellular matrix proteins or polymers that enhance their ability to attach to culture plates. Polyethyleneimine is a posit...

    Authors: Ajith R Vancha, Suman Govindaraju, Kishore VL Parsa, Madhuri Jasti, Maribel González-García and Rafael P Ballestero
    Citation: BMC Biotechnology 2004 4:23
    Content type: Methodology article Published on:

  34. Bioleaching is a process that has been used in the past in mineral pretreatment of refractory sulfides, mainly in the gold, copper and uranium benefit. This technology has been proved to be cheaper, more effic...

    Authors: Mario Makita, Margarita Esperón, Benito Pereyra, Alejandro López and Erasmo Orrantia
    Citation: BMC Biotechnology 2004 4:22
    Content type: Research article Published on:

  35. Cell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in ...

    Authors: Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood and Timothy J Mitchison
    Citation: BMC Biotechnology 2004 4:21
    Content type: Methodology article Published on:

  38. RNA interference (RNAi) in animals and post-transcriptional gene silencing (PTGS) in plants are related phenomena whose functions include the developmental regulation of gene expression and protection from tra...

    Authors: Brian Reavy, Sheila Dawson, Tomas Canto and Stuart A MacFarlane
    Citation: BMC Biotechnology 2004 4:18
    Content type: Research article Published on:

  39. There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to...

    Authors: Douglas L Sheridan and Thomas E Hughes
    Citation: BMC Biotechnology 2004 4:17
    Content type: Methodology article Published on:

  40. Polycistronic retroviral vectors that contain several therapeutic genes linked via internal ribosome entry sites (IRES), provide new and effective tools for the co-expression of exogenous cDNAs in clinical gen...

    Authors: Victorine Douin, Stephanie Bornes, Laurent Creancier, Philippe Rochaix, Gilles Favre, Anne-Catherine Prats and Bettina Couderc
    Citation: BMC Biotechnology 2004 4:16
    Content type: Research article Published on:

  41. Sustainable DNA resources and reliable high-throughput genotyping methods are required for large-scale, long-term genetic association studies. In the genetic dissection of common disease it is now recognised t...

    Authors: Rebecca Pask, Helen E Rance, Bryan J Barratt, Sarah Nutland, Deborah J Smyth, Meera Sebastian, Rebecca CJ Twells, Anne Smith, Alex C Lam, Luc J Smink, Neil M Walker and John A Todd
    Citation: BMC Biotechnology 2004 4:15
    Content type: Methodology article Published on:

  43. In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. The moss Physcomitrella patens has become an exciting model ...

    Authors: Verena Horstmann, Claudia M Huether, Wolfgang Jost, Ralf Reski and Eva L Decker
    Citation: BMC Biotechnology 2004 4:13
    Content type: Research article Published on:

  44. RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited ...

    Authors: Fekadu Yadetie, Arne K Sandvik, Hallgeir Bergum, Kristin Norsett and Astrid Laegreid
    Citation: BMC Biotechnology 2004 4:12
    Content type: Methodology article Published on:

  45. Electroporation (EP) is a widely used non-viral gene transfer method. We have attempted to develop an exact protocol to maximize DNA expression while minimizing tissue damage following EP of skeletal muscle in vi...

    Authors: Jordan Taylor, Charlie F Babbs, Mohammed Borhan Alzghoul, Aaron Olsen, Mickey Latour, Amber L Pond and Kevin Hannon
    Citation: BMC Biotechnology 2004 4:11
    Content type: Methodology article Published on:

  46. Members of the GATA transcription factor family have been used in many transfection studies to investigate their roles in the regulation of gene expression. To correct for variations in transfection efficiency...

    Authors: Clement KM Ho and Jerome F Strauss III
    Citation: BMC Biotechnology 2004 4:10
    Content type: Research article Published on:

  47. Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the...

    Authors: Marina Sudomoina, Ekaterina Latypova, Olga O Favorova, Erica A Golemis and Ilya G Serebriiskii
    Citation: BMC Biotechnology 2004 4:9
    Content type: Methodology article Published on:

  48. Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either g...

    Authors: Susanna Harju, Halyna Fedosyuk and Kenneth R Peterson
    Citation: BMC Biotechnology 2004 4:8
    Content type: Methodology article Published on:

  50. Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from ot...

    Authors: Wouter van Wyngaardt, Teresiah Malatji, Cordelia Mashau, Jeanni Fehrsen, Frances Jordaan, Dubravka Miltiadou and Dion H du Plessis
    Citation: BMC Biotechnology 2004 4:6
    Content type: Research article Published on:
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