Brazzolotto X, Wandhammer M, Ronco C, Trovaslet M, Jean L, Lockridge O, Renard PY, Nachon F. Human butyrylcholinesterase produced in insect cells: huprine-based affinity purification and crystal structure. FEBS J. 2012;279(16):2905–16. doi:10.1111/j.1742-4658.2012.08672.x.
Article
CAS
Google Scholar
Brimijoin S, Gao Y, Anker JJ, Gliddon LA, LaFleur D, Shah R, Zhao Q, Singh M, Carroll ME. A cocaine hydrolase engineered from human butyrylcholinesterase selectively blocks cocaine toxicity and reinstatement of drug seeking in rats. Neuropsychopharmacology. 2008;33(11):2715–25.
Article
CAS
Google Scholar
Chakrabarty R, Banerjee R, Chung SM, Farman M, Citovsky V, Hogenhout SA, Tzfira T, Goodin M. PSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions. Mol Plant Microbe Interact. 2007;20(7):740–50. doi:10.1094/MPMI-20-7-0740.
Article
CAS
Google Scholar
Chen X, Xue L, Hou S, Jin Z, Zhang T, Zheng F, Zhan C-G. Long-acting cocaine hydrolase for addiction therapy. Proc Natl Acad Sci U S A. 2016;113:422–7.
Article
CAS
Google Scholar
Cohen-Barak O, Wildeman J, van de Wetering J, Hettinga J, Schuilenga-Hut P, Gross A, Clark S, Bassan M, Gilgun-Sherki Y, Mendzelevski B, Spiegelstein O. Safety, Pharmacokinetics, and Pharmacodynamics of TV-1380, a Novel Mutated Butyrylcholinesterase Treatment for Cocaine Addiction, After Single and Multiple Intramuscular Injections in Healthy Subjects. J Clin Pharmacol. 2015;55:573–83.
Article
CAS
Google Scholar
Ersche KD, Jones PS, Williams GB, Turton AJ, Robbins TW, Bullmore ET. Abnormal brain structure implicated in stimulant drug addiction. Science. 2012;335:601–4.
Article
CAS
Google Scholar
Geyer BC, Kannan L, Cherni I, Woods RR, Soreq H, Mor TS. Transgenic plants as a source for the bioscavenging enzyme human butyrylcholinesterase. Plant Biotechnol J. 2010;8(8):873–86. doi:10.1111/j.1467-7652.2010.00515.x.
Article
CAS
Google Scholar
Geyer BC, Kannan L, Garnaud PE, Broomfield CA, Cadieux CL, Cherni I, Hodgins SM, Kasten SA, Kelley K, Kilbourne J, Oliver ZP, Otto TC, Puffenberger I, Reeves TE, Robbins 2nd N, Woods RR, Soreq H, Lenz DE, Cerasoli DM, Mor TS. Plant-derived human butyrylcholinesterase, but not an organophosphorous-compound hydrolyzing variant thereof, protects rodents against nerve agents. Proc Natl Acad Sci U S A. 2010;107(47):20251–6. doi:10.1073/pnas.1009021107.
Article
CAS
Google Scholar
Geyer BC, Woods RR, Mor TS. Increased organophosphate scavenging in a butyrylcholinesterase mutant. Chem Biol Interact. 2008;175(1–3):376–9.
Article
CAS
Google Scholar
Hofgen R, Willmitzer L. Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 1988;16(20):9877.
Article
CAS
Google Scholar
Hou S, Xue L, Yang W, Fang L, Zheng F, Zhan C-G. Substrate selectivity of high-activity mutants of human butyrylcholinesterase. Org Biomol Chem. 2013;11:7477–85.
Article
CAS
Google Scholar
Huang YJ, Huang Y, Baldassarre H, Wang B, Lazaris A, Leduc M, Bilodeau AS, Bellemare A, Cote M, Herskovits P, Touati M, Turcotte C, Valeanu L, Lemee N, Wilgus H, Begin I, Bhatia B, Rao K, Neveu N, Brochu E, Pierson J, Hockley DK, Cerasoli DM, Lenz DE, Karatzas CN, Langermann S. Recombinant human butyrylcholinesterase from milk of transgenic animals to protect against organophosphate poisoning. Proc Natl Acad Sci U S A. 2007;104(34):13603–8. doi:10.1073/pnas.0702756104.
Article
CAS
Google Scholar
Landry DW, Zhao K, Yang GX, Glickman M, Georgiadis TM. Antibody-catalyzed degradation of cocaine. Science. 1993;259(5103):1899–901. doi:10.1126/science.8456315.
Article
CAS
Google Scholar
Larrimore KE, Barcus M, Kannan L, Gao Y, Zhan C-G, Brimijoin S, Mor T. Plants as a source of butyrylcholinesterase variants designed for enhanced cocaine hydrolase activity. Chem Biol Interact. 2013;203:217–20.
Article
CAS
Google Scholar
Milton AL, Everitt BJ. Wiping drug memories. Science. 2012;336:167–8.
Article
CAS
Google Scholar
Nicolet Y, Lockridge O, Masson P, Fontecilla-Camps JC, Nachon F. Crystal structure of human butyrylcholinesterase and of its complexes with substrate and products. J Biol Chem. 2003;278:41141–7.
Article
CAS
Google Scholar
Pan Y, Gao D, Yang W, Cho H, Yang G, Tai H-H, Zhan C-G. Computational redesign of human butyrylcholinesterase for anticocaine medication. Proc Natl Acad Sci U S A. 2005;102(46):16656–61. doi:10.1073/pnas.0507332102.
Article
CAS
Google Scholar
Restrepo MA, Freed DD, Carrington JC. Nuclear transport of plant potyviral proteins. Plant Cell. 1990;2:987–98.
Article
CAS
Google Scholar
Schneider JD, Castilho A, Neumann L, Altmann F, Loos A, Kannan L, Mor TS, Steinkellner H. Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue. Biotechnol J. 2014;9:501–10.
Article
CAS
Google Scholar
Shram MJ, Cohen-Barak O, Chakraborty B, Bassan M, Schoedel KA, Hallak H, Eyal E, Weiss S, Gilgun Y, Sellers EM, Faulknor J, Spiegelstein O. Assessment of pharmacokinetic and pharmacodynamic interactions between albumin-fused mutated butyrylcholinesterase and intravenously administered cocaine in recreational cocaine users. J Clin Psychopharmacol. 2015;35:396–405.
Article
CAS
Google Scholar
Skolnick P, White D, Acri JB. Editorial: emerging targets for stimulant use disorders: where to invest in an era of constrained resources? CNS Neurol Disord Drug Targets. 2015;14:691.
Article
CAS
Google Scholar
Sun H, Pang Y-P, Lockridge O, Brimijoin S. Re-engineering butyrylcholinesterase as a cocaine hydrolase. Mol Pharmacol. 2002;62(2):220–4.
Article
CAS
Google Scholar
Willyard C. Quest for the quitting pill. Nature. 2015;522:S53.
Article
CAS
Google Scholar
Xue L, Hou S, Tong M, Fang L, Chen X, Jin Z, Tai H-H, Zheng F, Zhan C-G. Preparation and in vivo characterization of a cocaine hydrolase engineered from human butyrylcholinesterase for metabolizing cocaine. Biochem J. 2013;453:447–54.
Article
CAS
Google Scholar
Xue L, Ko M-C, Tong M, Yang W, Hou S, Fang L, Liu J, Zheng F, Woods JH, Tai H-H, Zhan C-G. Design, preparation, and characterization of high-activity mutants of human butyrylcholinesterase specific for detoxification of cocaine. Mol Pharmacol. 2011;79:290–7.
Article
CAS
Google Scholar
Yang W, Xue L, Fang L, Chen X, Zhan C-G. Characterization of a high-activity mutant of human butyrylcholinesterase against (−)-cocaine. Chem Biol Interact. 2010;187(1–3):148–52.
Article
CAS
Google Scholar
Zheng F, Xue L, Hou S, Liu J, Zhan M, Yang W, Zhan C-G. A highly efficient cocaine-detoxifying enzyme obtained by computational design. Nat Commun. 2014;5:3457. doi: 3410.1388/ncomms4457.
Google Scholar
Zheng F, Yang WC, Ko MC, Liu JJ, Cho H, Gao DQ, Tong M, Tai HH, Woods JH, Zhan C-G. Most efficient cocaine hydrolase designed by virtual screening of transition states. J Am Chem Soc. 2008;130(36):12148–55. doi:10.1021/ja803646t.
Article
CAS
Google Scholar
Zheng F, Zhan C-G. Are pharmacokinetic approaches feasible for treatment of cocaine addiction and overdose? Future Med Chem. 2012;4:125–8.
Article
CAS
Google Scholar