Fig. 4

In vivo profiles of pCocH3-Fc. a Time-dependent concentrations of the active enzyme pCocH3-Fc or PEG-pCocH3-Fc (PEGylated pCocH3-Fc) in the plasma of mice after IV administration of the enzyme (0.075 mg/kg) determined in triplicate. b Lethality of 180 mg/kg cocaine (IP) with or without pretreatment of 1 mg/kg pCocH3-Fc. The lethality tests were performed in triplicate (n = 3 for each group). For the mice survived from the acute cocaine toxicity testing, the observation of animal behaviors was kept for at least 1 h to make sure that they continued to behave normally after the survival