Fig. 2
From: Plant expression of cocaine hydrolase-Fc fusion protein for treatment of cocaine abuse
Expression profiles of pCocH3 and pCocH3-Fc. For each protein, three leaves per day were harvested from the same layer of three independent plants on the indicated time, and mixed together for enzyme extraction. The crude extracts prepared from leaves were used for the initial enzyme activity assays (in triplicate). The protein concentration was calculated based on the measured enzyme activity (Vmax) and the known catalytic parameter (k cat = 5700 min−1)