Articles

Long term adaptation of a microbial population to a permanent metabolic constraint: overcoming thymineless death by experimental evolution of Escherichia coli

To maintain populations of microbial cells under controlled conditions of growth and environment for an indefinite duration is a prerequisite for experimentally evolving natural isolates of wild-type species o...

Authors: Valérie A de Crécy-Lagard, Jacques Bellalou, Rupert Mutzel and Philippe Marlière

L-Glutamate production by lysozyme-sensitive Corynebacterium glutamicum ltsAmutant strains

A non-pathogenic species of coryneform bacteria, Corynebacterium glutamicum, was originally isolated as an L-glutamate producing bacterium and is now used for fermentative production of various amino acids. A mut...

Authors: Takashi Hirasawa, Masaaki Wachi and Kazuo Nagai

A simple method for statistical analysis of intensity differences in microarray-derived gene expression data

Microarray experiments offer a potent solution to the problem of making and comparing large numbers of gene expression measurements either in different cell types or in the same cell type under different condi...

Authors: Alexander Kamb and Mani Ramaswami

Mutant loxP vectors for selectable marker recycle and conditional knock-outs

Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used as a model for gene knock-outs due...

Authors: Hiroshi Arakawa, Dimitry Lodygin and Jean-Marie Buerstedde

A surrogate-based approach for post-genomic partner identification

Modern drug discovery is concerned with identification and validation of novel protein targets from among the 30,000 genes or more postulated to be present in the human genome. While protein-protein interactio...

Authors: Renuka C Pillutla, Ku-chuan Hsiao, Renee Brissette, Paul S Eder, Tony Giordano, Paul W Fletcher, Michael Lennick, Arthur J Blume and Neil I Goldstein

Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G

There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However...

Authors: Jae Hong Im, Takashi Nakane, Hiroshi Yanagishita, Toru Ikegami and Dai Kitamoto

Oriented antibody immobilization to polystyrene macrocarriers for immunoassay modified with hydrazide derivatives of poly(meth)acrylic acid

Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromol...

Authors: Vadim V Shmanai, Tamara A Nikolayeva, Ludmila G Vinokurova and Anatoli A Litoshka

Erratum to: The evaluation of yeast derivatives as adjuvant of the immune response to the Bm86 antigen in cattle: Correction

Authors: Manuel Rodríguez Valle, Carlos Montero, Héctor Machado and Marisdania Joglar

The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new a...

Authors: Manuel Rodríguez Valle, Carlos Montero, Héctor Machado and Marisdania Joglar

Inhibition of spontaneous induction of lambdoid prophages in Escherichia colicultures: simple procedures with possible biotechnological applications

Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli i...

Authors: Agata Czyz, Marcin Los, Borys Wrobel and Grzegorz Wegrzyn