Skip to main content
Download PDF

A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile

BMC Biotechnology volume 20, Article number: 12 (2020) Cite this article

Abstract

Background

Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant biomaterials. When bagasse is stored in piles in the open for long periods it is colonised by microbes originating from the sugarcane, the soil nearby or spores in the environment. For these microorganisms to proliferate they must digest the bagasse to access carbon for growth. The microbial community in bagasse piles is thus a potential resource for the discovery of useful and novel microbes and industrial enzymes. We used culturing and metabarcoding to understand the diversity of microorganisms found in a uniquely undisturbed bagasse storage pile and screened the cultured organisms for fibre-degrading enzymes.

Results

Samples collected from 60 to 80 cm deep in the bagasse pile showed hemicellulose and partial lignin degradation. One hundred and four microbes were cultured from different layers and included a high proportion of oleaginous yeast and biomass-degrading fungi. Overall, 70, 67, 70 and 57% of the microbes showed carboxy-methyl cellulase, xylanase, laccase and peroxidase activity, respectively. These percentages were higher in microbes selectively cultured from deep layers, with all four activities found for 44% of these organisms. Culturing and amplicon sequencing showed that there was less diversity and therefore more selection in the deeper layers, which were dominated by thermophiles and acid tolerant organisms, compared with the top of pile. Amplicon sequencing indicated that novel fungi were present in the pile.

Conclusions

A combination of culture-dependent and independent methods was successful in exploring the diversity in the bagasse pile. The variety of species that was found and that are known for biomass degradation shows that the bagasse pile was a valuable selective environment for the identification of new microbes and enzymes with biotechnological potential. In particular, lignin-modifying activities have not been reported previously for many of the species that were identified, suggesting future studies are warranted.

Background

Bagasse is the fibrous material remaining after sugarcane stalks are crushed to remove the sugar and is a major source of lignocellulose. In 2018 for example, 188 million tons of sugar was produced around the world which would have produced approximately 180–200 million tons of bagasse [1].

At most mills the bagasse is used to fuel boilers, co-generating steam and electricity [2]. However it could have various higher value uses: second generation biofuels [3]; fibres for paper, particle board [4] and in 3D printing [5]; xylan-based products such as xylooligosaccharides [6]; substrate for edible or medicinal mushroom growth [7]; substrate for single-cell protein, enzyme, or other high-value microbial products [8, 9]; and high value chemicals from the lignin fraction [10] are some examples. Bagasse has advantages such as low ash content (~ 2.5% compared to 11–14% in other plants, [9]) and as a C4 crop, sugarcane is one of the most efficient converters of energy into biomass. However, the potential for value-adding to bagasse and other lignocellulose feedstocks is primarily limited by the recalcitrant nature of the polymers present and the lack of efficient and sustainable (economic and environmental) conversion processes. Biological processing is one approach and although progress in our understanding of biomass decomposition is continually improving, there is still potential to further exploit the genetic diversity of adapted microbial communities inhabiting environments rich in lignocellulose.

When bagasse leaves the mill it contains approximately 50% water and of the remaining dry matter, 50% cellulose, 25% hemicellulose, 25% lignin, a small percentage of sucrose and very little nitrogen (0.1–0.5%) [11]. Excess bagasse is often stored in large open-air stockpiles. Several studies have shown that these piles become unique environmental niches with distinctive micro-conditions, such as gradients in temperature, pH and oxygen, and diverse microbial populations depending on the position in the pile and/or age of the bagasse [12,13,14,15,16]. Culturing [17,18,19,20,21], metabarcoding, and metagenomics [13, 14, 22,23,24,25,26,27] on bagasse pile samples or associated soil has revealed a unique microbial community compared to other lignocellulosic environments and the presence of novel biomass-degrading microbes and enzymes. In addition, composting-like conditions are more efficient than submerged fermentation for enriching organisms with cellulases [28] and presumably other carbohydrate hydrolases. Mello et al., also found that a microbial consortium grown under nutrient limiting conditions on sugarcane bagasse, became more diverse and enriched in lignocellulose-degrading enzymes than the same consortium grown on rich media [29]. However, there is still much to learn about bagasse-enriched microbes, especially fungi, because at present the contribution of their lignocellulolytic enzymes to biomass degradation has been underestimated since both their presence and role has not been extensively investigated in relevant metagenomic studies. Indeed, Mhuantong et al., [13] observed that a relatively small proportion of a bagasse fosmid library represented fungal DNA (less than 4.3% of total) but that all of the lytic polysaccharide monooxygenases (CaZy AA9) in the sample were of fungal origin.

Previous bagasse microbe studies have focused on either culturing or extensive sequence-based analyses. There is benefit however to using both approaches [30,31,32]. While culture-independent methods have come to the fore due to the estimate that approximately 99% of microbes cannot be cultured [33], culturing is still necessary for understanding microbial function, physiology, micro-diversity and community dynamics in the environment [34] and to be able to use the microbes and associated enzymes in industrial processes. Both methods also have biases. Culturing depends on the choice of media and conditions and microbes that are not really living in the pile can be cultured from spores. With PCR-based methods, DNA from non-viable organisms can be amplified and the primers can amplify certain sequences better than others. The DNA extraction step can also bias certain organisms and according to human microbiome studies, for sequencing, microbes should be present at 105 cells/mL whereas they can be cultured from as low as 102 cells/mL [32]. Finally, for both methods but especially culture-independent studies, microbe identification relies on database quality and completeness.

The aim of this study was to explore the diversity of microbes in bagasse and their potential for lignocellulose conversion. In contrast to previous studies, we used both culture dependent and culture-independent methods to describe the fungi and bacteria associated with different positions in a relatively small and uniquely undisturbed Australian bagasse pile. We sampled at different positions because the pile was deposited gradually over several sugarcane milling seasons so that bagasse deeper in the pile had been there longer. However due to the large size of the pile, we were only aiming for a ‘snapshot’ of the organisms present, to explore the potential of this environment, rather than a comprehensive analysis, as this was practically not feasible. In a first step towards characterising their function and potential for novel enzyme activities, we screened the cultured microbes for lignocellulose-degrading enzymes, including lignin-modifying enzymes.

Results

Bagasse sampling and analysis

Seven samples were collected in different positions from the bagasse stockpile at the Rocky Point mill in Queensland Australia as detailed in Fig. 1a and Table 1. The temperature ranged from approximately 50 °C (Table 1) in the deeper layers that we sampled to 30 °C on the surface. The pH was low in the first deep layer sample we collected but the deep layer was of similar pH to the other layers 6 months later (Table 1), suggesting temporal and environmental variability at equivalent depths across the pile. The chemical compositional analysis showed notable changes in the deep samples and especially the oldest and deepest sample (Sample 4) compared to other samples. Sample 1, and to a further extent, Sample 4 also showed visual signs of modification and degradation (Fig. 1a). The sugars issuing from hemicellulose, arabinan and galactan were significantly lower (p < 0.001) in Samples 1, 4 and 5, and xylan, was also significantly lower (p < 0.05) in Sample 4 than in the other samples (Fig. 1b, c). Acid soluble lignin contents were also significantly lower (p < 0.05) in the deep samples compared to the other samples (Fig. 1d). These differences could be due to microbial activity combined with the low pH of the pile leading to degradation. Glucan, on the other hand, was proportionally higher in both samples from the deeper layers suggesting that cellulose or the glucan component of hemicellulose was less degraded than the C5 components of hemicellulose.

Fig. 1
figure 1

Bagasse sampling and compositional analysis. a Photos of the Rocky Point bagasse pile and samples taken at different positions in the pile. b Glucan and xylan as % of total solids in five different bagasse samples. Glucan was significantly higher (p < 0.05) in Samples1 and 4 compared to others and xylan was significantly lower (p < 0.05) in Sample 4 compared to others. c Galactan and arabinan as % of total solids in five different bagasse samples. Both were significantly lower in Samples 1 and 4 compared to others (p < 0.01). d Acid insoluble and soluble lignin as % of total solids in five different bagasse samples. Acid soluble lignin was significantly lower in Samples 1 and 4 (p < 0.05). Error is standard deviation and n = 3

Full size image
Table 1 Characteristics of bagasse samples from the Rocky Point sugarcane mill
Full size table

Oil-producing yeast, biomass-degrading fungi, Bacillus and Streptomyces cultured from the bagasse

In total, 104 microbes were cultured from bagasse samples collected at the Rocky Point sugarcane mill in May 2016 and February 2017. The strains and how they were selected are summarised in Additional file 1. 16S or ITS sequences were used to query the 16S ribosomal sequence (bacterial and archaeal) database at NCBI or the UNITE [35] database, respectively. The top BLAST hit based on e-values was noted even though in some cases the sequence matched several sequences in the database with the same percentage identity.

The microbes were isolated in two separate rounds of culturing. The samples were rinsed to remove spores on the surface and the samples were ground in Tween detergent to isolate organisms strongly adhered to the bagasse. In the first round, fresh samples were incubated on rich media and isolates compared between three samples from the top (Sample 2), 10 cm under the crust (Sample 3) and 60 cm deep (Sample 1), with a focus on yeast and filamentous fungi. Indeed, Bacillus dominated plates without chloramphenicol and these were the only bacteria isolated besides one Burkholderia species (RP31) which was resistant to chloramphenicol. Only four Bacillus isolates (B. amyloliquefaciens and B. megaterium; nominated as RP1, RP2, RP3 and RP5) and one fungal species, closest to Talaromyces flavus (RP4) were cultured from the deep sample (1). From the top of the pile, yeast from six different genera and filamentous fungi from seven different genera were cultured. Four yeast and six fungi were cultured from the 10 cm sample (Additional file 1).

Next, selective plating was carried out with the aim of isolating thermophilic and mesophilic biomass-degrading enzyme producing organisms. For this, new bagasse samples were obtained from 80 cm deep (Sample 4), which as mentioned above appeared to be substantially degraded, and we also cultured a sample from the surface with obvious fungal growth (Sample 7). Forty-eight microorganisms including bacteria (10), yeast (14) and filamentous fungi (24) were cultured from the 80 cm sample. Apart from Bacilli, the only bacteria isolated were Streptomyces (Streptomyces mexicanus and clavus, RP52, RP53, RP64 and RP81), with S. mexicanus seemingly outcompeting or inhibiting all other microorganism when grown on carboxy-methyl cellulose at 50 °C. It was also the only isolate that grew alongside Thielavia species on xylan at 50 °C. Thielavia species also grew on rich media at 50 °C as well as on cellulose and xylan as the sole carbon sources. However, when bagasse was included in the media and the plates were incubated at 50 °C, the fast-growing Aspergillus fumigatus outgrew any other organism. At 28 °C, the Aspergillus was outcompeted by Talaromyces. Similarly, at the lower temperature, yeast outgrew Streptomyces and filamentous fungi on Azo-xylan media. Twelve fungi from the top of the pile grew on lignin-containing media at 28 °C.

In the selective culturing some isolates of the same species were cultured under more than one condition (Additional file 1). For example, strains most similar to Thielavia terrestris (RP40, 41, 43, 44, 45, 54, 76, 77, 78, 80, 81, 83) were cultured at 50 °C, on CMC, xylan and guaiacol. While most of the partial 16S or ITS sequences of the cultured strains were 99–100% identical to a sequence of a known species in the databases, some showed slightly less similarity (Additional file 1). The rRNA sequence of one isolated organism (RP12) showed similarity to a sequence only identified in the databases as Ascomycota sp. but its morphology more resembled Coniochaeta/Lecythophora. Two other Coniochaeta strains (RP62 and RP68) also appeared to be quite different from those with sequences in the databases and their partial rRNA sequences were also deposited at NCBI. Two strains whose morphology resembled Rhizopus (RP38 and RP94) and one with similarity to Mucoromycotina (RP34) could not be sequenced with these primer sets (Additional file 1).

Many of the yeast isolates cultured from the bagasse are reported to accumulate high levels of microbial oil, for example Rhodoturola/Rhodosporidium (RP7, 8, 14, 15, 28, 58, 66, 67 and 69) [36, 37], Cryptococcus/Papiliotrema laurentii (RP13, 27, 29, 61, 65 and 70) [38] and Meyerozyma caribbica (RP6, 16 and 30) [39] as well as other closely related species such as Naganishia (RP60).

Enzyme screening of bagasse microbes

All 104 isolates were screened in semi-quantitative assays on solid media for the production of biomass-degrading enzymes (Fig. 2b). The results are summarised in Fig. 2a and an example of each assay is shown in Fig. 2b. Overall, 70, 67, 70 and 57% of the isolated microorganisms showed CMCase, xylanase, laccase and peroxidase activity, respectively and approximately 29% displayed all four activities. There was little difference in the proportion of fungi from the top or 10 cm deep layers that produced biomass-degrading enzymes. For example, 38 and 31% of the fungi isolated from Sample 2 and 3 showing xylanase activity. However, 100% (5 microorganisms) from Sample 1 produced CMCase and xylanase and in Sample 4, xylanase activity was found for 80% of the microorganisms, CMCase for 85%, laccase for 67% and peroxidase for 73% of organisms. All four activities were found for 44% of Sample 4 organisms.

Fig. 2
figure 2

Screening of bagasse microbes for biomass-degrading enzymes. a The bacteria, yeast and filamentous fungi (RP1-RP103) isolated from different positions in the pile were screened for cellulase, xylanase, laccase and peroxidase on carboxy-methyl cellulose with trypan blue dye, azo-xylan, remazol brilliant blue and azure B containing agar plates, respectively. Activity was scored as positive or not based on clearance zones around the colonies. Low activity indicates a very minor clearance zone that took longer than 7 days to become visible. Examples of each assay are shown in b

Full size image

To confirm the functional presence of these organisms in the bagasse pile, we grew the 44 strains that were positive on xylan agar plates from the deep layers (Samples 1 and 4) in minimal media containing bagasse as the sole carbon source and measured xylanase activity. Almost all the strains showed some level of activity, which approximately correlated with the level of activity on agar plates (based on the size of the clearance zone and speed of clearance), validating the screening approach (Fig. 3a). The highest activities were shown by Aspergillus terreus (RP84/85) [40], Rasamsonia emersonii (RP42/82), Aspergillus fumigatus (RP74/75), Thielavia terrestris and the as yet uncharacterised xylanase producers Talaromyces rugulosus (RP4), Coniochaeta taeniospora (RP62/68) [41] and Streptomyces mexicanus (RP51) [42]. Westerdykella dispersa (RP86) was the only definite ‘false-positive’ with obvious growth but no xylanase activity even after 7 days in bagasse media. To confirm that the bagasse was inducing the activity, several strains were grown in media with and without bagasse or glucose and activity was the highest with bagasse as the only carbon source (Fig. 3b).

Fig. 3
figure 3

Bagasse induces xylanase activity among the isolates. a Xylanase activity was measured for all the strains isolated from the deep layers (Samples 1 and 4) that showed activity in the plate assay. The assay was carried out on strains grown in 24 well plates for six days in yeast nitrogen base (YNB) media containing 2% sugarcane bagasse as the carbon source. b Xylanase activity of a selection of the strains in YNB media with and without bagasse and glucose after seven days, showing that the bagasse induced xylanase. Xylanase activity is expressed as units per ml. Error is standard deviation and N = 3 in the assay

Full size image

Amplicon sequencing-overview

Amplicon sequencing was used to rapidly gain insight into the diversity of microbes present in the bagasse pile, especially those that were not cultured. With16S primers, a total of 608,084 three-hundred base pair pair-end reads were obtained with approximately 56,000 to 120,000 reads per sample. A total of 347,222 reads were obtained from ITS primer sequencing, ranging from approximately 55,000 (Sample 3) to 88,000 reads for Sample 2. Presumably due to the low microbial load in the initial deep layer sample (Sample 1) as shown by culturing, no products were amplified with the ITS primers. The number of reads amplified with 16S primers from this sample was also much lower than for the other samples (approximately 56,000 versus 100,000 to 120,000 for others). The reads were submitted to the NCBI Short Read Archive (SRA) under BioProject ID PRJNA530327 according to the minimum information about any (x) sequence (MIxS) specifications [43].

In all the samples, 1747 different bacterial and 363 fungal sequences were amplified (Additional files 2 and 3). The number of operational taxonomic units (OTUs) for each sample ranged from 332 (Sample 1, deep layer) to 787 (Sample 2, top layer) for 16S sequencing and 67 (Sample 4, deep layer) to 285 (Sample 2, top layer) for ITS, reflecting, as shown in Figs. 4 and 7, that there was more diversity in the top than deeper layers of the bagasse pile.

Fig. 4
figure 4

Rarefaction curves for 16S and ITS amplicon sequences for each sample. a Rarefaction curve for 16S amplicons. Species richness was highest in Sample 2 and lowest in Samples 1 and 4. b Rarefaction curve for ITS amplicons. Species richness was highest in Sample 2 and lowest in Sample 4

Full size image

Taxonomic classification (Additional files 4 and 5) showed overall that 19 bacterial phyla were found in the bagasse pile with Proteobacteria (~ 23%), Actinobacteria (~ 17%), Firmicutes (~ 17%) and Acidobacteria (~ 11%) dominating (Fig. 5a).

Fig. 5
figure 5

Proportion of major bacterial phyla found in the bagasse samples according to amplicon sequencing. a Overall classification of 16S amplicons assigned to operational taxonomic units at the phylum level from all samples. b Classification of 16S amplicons for each sample

Full size image

Approximately 87% of the fungi identified were Ascomycota and 10% Basidiomycota, with a small proportion of Zygomycota and unidentified/unclassified fungi (Fig. 6a). The Basidiomyces were mostly found in Sample 5 (30 cm depth) (Fig. 6b).

Fig. 6
figure 6

Proportion of fungal phyla found in the bagasse samples according to amplicon sequencing. a Overall classification of ITS amplicons assigned to operational taxonomic units at the phylum level from all samples. b Classification of ITS amplicons for each sample

Full size image

At the class level, 45% of the total reads were unclassified Ascomycota (Additional file 5). However, among the classified reads, the Eurotiomycetes (32% of total; for e.g. Rasamsonia, Talaromyces, Aspergillus) dominated across all samples followed by the Agaricomycetes (10% of total) which accounted for almost all the Basidiomycota. Lastly, Dothideomycetes were found to represent about 5% of the total reads and were mostly in Sample 2 (top layer).

Bacterial diversity in the pile – dominance of thermophiles and acidophiles

OTUs were classified to the genus level for bacteria (Fig. 7a; Additional file 4). The highest total number of bacterial reads, due to their abundance in the two deep layer samples (1 and 4), were Alicyclobacillus. This thermo- and acid-tolerant genus was also found in Thai bagasse [11], has well characterised biomass degrading capacity, particularly of xylan [44, 45] and also thrives on both pentose and hexose sugars [46]. However, it has been more commonly associated with fruit juice contamination rather than any other environment. At the species level, the most abundant reads were 99% identical to the newly identified species Alicyclobacillus kakegawensis [47] and 98% identical to 16S of the type strain Alicyclobacillus acidocaldarius [48]. Overall, 12 different Alicyclobacillus sequences were amplified from the samples (Additional file 2).

Fig. 7
figure 7

Major bacterial genera and fungal species found in the bagasse samples according to amplicon sequencing. a Top 10 most abundant bacteria at the genus level showing their abundance in each sample. b Top 10 most abundant fungi at the species level showing their abundance in each sample

Full size image

An unclassified Acidobacteria was also very abundant in the deep samples and samples under the crust but not in the top samples (Fig. 7a). The sequence of the representative read for this classification was 100% identical to that of another newly identified thermotolerant species, Acidobacterium ailaaui, from a geothermally heated Hawaiian microbial mat [49]. This strain can grow from pH 4.5 to 6, at 15 °C to 55 °C and can metabolise xylose and arabinose. Finally, the third most abundant bacteria, found mostly in Sample 2 (top) belongs to the Chloflexi phyla and is 92% identical to the Ktedonobacteria (Fig. 5a) Thermosporothrix narukonensis and Thermosporothrix hazakensis, which are both thermophilic and able to hydrolyse cellulose and xylan [50, 51].

In terms of other bacteria in the pile that may be producing biomass-degrading enzymes, in a previous study [13] all the major phyla in the pile were producing hemicellulose and cellulose degrading enzymes but lignin-modifying enzymes were mostly being produced by Bacteroidetes and Proteobacteria. However, in the present study Bacteroidetes were not as abundant as Firmicutes and Actinobacteria and in Sample 4 the lignin degrading bacteria Amycolatpsis [52] was abundant. We also found a relatively high abundance of Sulfobacillus from the Clostridia family in our deep layer samples. The other most abundant bacteria sequenced are shown in Fig. 7a and listed in Additional files 2 and 4.

Fungal diversity in the pile –ubiquitous biomass degraders and novel organisms

ITS OTUs were classified using the UNITE database. The most abundant fungi found in all five samples, with highest levels in Samples 3 and 6, were unclassified Ascomycota (Fig. 7b), represented by 54 different sequences (Additional file 3). Overall, 138 of the 370 unique amplicons correspond to uncharacterised fungi in UNITE and NCBI. Thirteen of these sequences, classified as unidentified fungi (UNITE SH230936.06FU/SH189980.06FU/SH211973.06FU) appear to be ciliated protozoa rather than fungi. Among the other 125, some (for example DENOVO1 in Additional file 3) had sequence homology with uncultured fungi from environmental samples but others could be individually classified with the most up-to-date version of UNITE (see below).

The second most abundant fungus, which was initially classified as unidentified Trichocomaceae sp. (Fig. 7b), was identified as Rasamsonia emersonii (previously Talaromyces emersonii) on more detailed individual analysis. This fungus was also cultured from the deep layer. R. emersonii is a well characterised moderately thermophilic fungus which produces thermostable xylanase and cellulase as well as other enzymes [53,54,55,56]. The strain isolated here produced all four enzymes and was a relatively high xylanase producer. Laccase and peroxidase production are reported for the first time here for this species.

The next most common fungal sequences amplified was for Basiomycota Hyphodontia palmae/dispersa from Sample 5 (accounting for the high Basiomycota reads from this sample). To date, this fungus is not well characterised.

Another abundant fungus among the sequences was Talaromyces piceus, found from the top of the pile at both sampling dates. Two other Talaromyces were also among the top hits (T. marneffei and T. radicus). The culturing experiments isolated the highly related T. ruber, T. amestolkiae, T. rugulosus, T. funiculosus and T. flavus, which all have very similar ITS sequences and appearances and are also anamorphs (or were renamed) to various Penicillium species [57]. While, T. piceus was recently shown to possess a novel lignocellulolytic enzyme system [58], T. amestolkiae produces diverse β-glucosidases [59, 60], and T. funiculosus (also known as P. funiculosum) is used commercially to degrade xylan in animal feed [61], the biomass degrading capability of the other isolates has not been reported until now.

The important industrial dimorphic yeast, Aureobasidium pullulans was sequenced and cultured from the top layer and produced all four enzymes [62,63,64]. Another well-known hemicellulose degrader Thermomyces lanuginosus [65] was also identified primarily in Sample 6 by sequencing. DNA from the white rot fungi Phanerochaete chrysosporium [66], which was never cultured, was amplified from samples from the top of the pile but not from the deeper samples.

Among the unknown Ascomycota sequences, the most abundant in Sample 4 was used to query the latest UNITE database and identified as Thielavia terrestris (accession KU729090), which, as mentioned above, was the major fungus cultured from this sample. Amplicon sequencing also showed that it was prevalent in samples closer to the top of the pile but not on the surface (DENOVO4 in Additional file 3). Most of the fungi that were cultured from Samples 1, 2, 3, 4 and 7 such as Aspergillus terreus, Aspergillus fumigatus, Alternaria, Fusarium, Coniochaeta, Rhodotorula, Exophilia, and Cryptococcus were represented in the amplicons at least at the genus level. We did not amplify Rhizopus or Paecilomyces, a genus of the phylum Deuteromycota, which as mentioned above were cultured and have been previously associated with sugarcane bagasse.

Discussion

In this study we assessed bagasse microbiota using a novel approach of both culturing and amplicon sequencing in combination with enzyme screening. In general, industrial bagasse piles are influenced by complex environmental factors. This pile was unique as it was relatively small and static over 9 months due to mill shutdown and thus had time for microbial communities and chemistries to develop. Normally, bagasse piles are transient, and bagasse is added or removed frequently. They are built up in uneven layers and each layer is also likely to have experienced different microbial loads during harvesting, processing, and deposition, and different conditions (rain or wind, dirty machinery, etc.) during deposition on the pile. Regardless, this study shows the potential of sugarcane bagasse piles for isolating novel biomass-degrading microbes and discovering new biomass-modifying enzymes. There may be the potential to develop methods to replicate, in the lab or field, the conditions which led to the bagasse colonisation and degradation and thus enrich for adapted microbes.

One hundred and four microbes were isolated from the bagasse pile in two separate rounds of culturing. The first non-selective round focused mostly on fungi and a second selective round aimed to specifically isolate biomass degraders. The strains isolated included Bacillus and Streptomyces bacterial species, a diverse range of yeast and known biomass-degrading filamentous fungi. Many of the strains belonged to the same species even though they showed different morphotypes on the same or different media. In the selective culturing some isolates of the same species were cultured under more than one condition (Additional file 1). Overall, the dynamics of populations observed under the different cultivation conditions used shows the importance of experimental design for such experiments and the relevance of our parallel genomic experiments to observe total populations.

The strains were named based on the high similarity of their rRNA sequences with known sequences in the databases, but high sequence conservation at the rRNA level does not preclude that the isolates may have evolved unique phenotypes/genotypes in response to environmental stimuli.

Many of the yeast isolates cultured from the bagasse such as Rhodoturola/Rhodosporidium [36, 37], Cryptococcus/Papiliotrema laurentii [38] and Meyerozyma caribbica [39] are reported to accumulate high levels of microbial oil. Indeed we demonstrated that the bagasse-derived strain RP15 (Rhodosporidium toruloides) produced higher yields of intracellular microbial oil when grown on pre-treated bagasse compared to synthetic media and compared to the ATCC type strain [67]. These yeast can grow with extremely low nitrogen concentrations [68], which may explain their prevalence in the nitrogen deficient bagasse pile, with low nitrogen media also used to induce microbial oil production. These, as well as other non-oleaginous yeast isolated, such as Candida, Aureobasidium, Coniochaeta/Lecythophora and Exophilia, may also be associated with sugarcane or soil in sugarcane plantations as many of the same species were also isolated from leaves and stems sampled close to the soil or from bagasse [13, 21].

Some of the other microbes isolated were also cultured previously from sugarcane and bagasse, such as Aspergillus fumigatus and Aspergillus niger (tubingensis), Paecilomyces, Tremella, Rasamsonia emersonii and others [12, 15, 17, 20, 21]. Thielavia terrestris is an efficient thermophilic biomass-degrader and a strain was previously isolated from Brazilian bagasse [19, 69]. Bacillus subtilis was prevalent in liquid samples collected at sugarcane mills with the authors proposing that it lives on sugarcane [70].

Amplicon sequencing gave rapid insight into the diversity of microbes present in the bagasse pile, especially those that were not cultured. Microbial diversity decreased in the deeper layers of the pile presumably due to increased specialisation. This decreased diversity was seen as variation in the distribution of bacteria and fungi from different phyla between samples (Figs. 5b and 6b). For example, Firmicutes dominated the deeper samples but were much less prevalent in top samples. These bacterial phyla also dominated in deeper layers of other piles under potentially similar environmental conditions (wet season) [14, 22]. However under potentially drier conditions (dry season) and in less degraded bagasse, Acidobacteria dominated [24].

There was less diversity among the fungi sequences that were amplified. However, ITS primer sequences for metabarcoding environmental DNA are still under development [71] and although the primers used here were selected based on their reported ability to amplify both Ascomycota and Basidiomycota [72, 73] primer specificity could have biased the DNA that was amplified [74].

The most abundant bacteria in the pile, Alicyclobacillus, Acidobacteria and Ktedonobacteria species are known thermophiles and acidophiles [46, 49, 51] showing adaptation to the conditions in the pile. The bacteria that we cultured from the bagasse pile, Bacillus, Streptomyces and Burkholderia were sequenced in all the samples but at relatively lower abundance. Bacillus reads were mostly amplified from Sample 1 (Additional file 2) but were still cultured from Sample 4. The differences in abundance between culturing and sequencing results also shows the benefit of performing both analyses in the same study.

The most abundant fungal sequences appeared to correspond to currently unidentified fungi, suggesting that novel fungi were present in the pile. However, the other highly abundant fungal sequences that were amplified were related to the thermophiles cultured in this study (R. ermersonii and T. terrestris as well as Thermomyces lanuginosus). In the only other culture-independent study of fungi in bagasse, Rattanachomsri et al., [14] sequenced 24 Ascomycota, including some that we also found, such as R. ermersonii and T. lanuginosaus. ITS amplicon sequencing of DNA extracted from a maize straw compost pile with added nitrogen found that T. lanuginosaus dominated the fungal population but there was also some Aspergillus and Talaromyces, as found here, among a few other genera [75].

The isolated organisms were screened for common lignocellulose degrading enzymes (cellulase, xylanase, laccase, peroxidase) and a large majority (up to 85%) of the strains expressed at least one enzyme, with 29 and 44% of organisms from the non-selective and selective rounds of culturing respectively, showing all four activities. The high proportions of microorganisms displaying hydrolase activity was also likely related to the selective enrichment in culturing for these activities. Overall, the microbes that had colonised the deeper parts of the bagasse pile where biomass modification was observed were mostly functionally specialised for lignocellulose decomposition and our targeted approach successfully enriched microorganisms with biomass degrading potential.

Under the conditions used here, especially in the first round of culturing, we mostly cultivated fast growing species. Other strategies such as plating on very dilute media or high throughput dilution plating could be used to isolate slower growing oligotrophs, but whether these would be better enzyme producers remains to be seen. Indeed, when such an approach was taken by Shrestha et al. [18], only 8 out of the 106 fungi they isolated from Miscanthus and sugarcane trash, could deconstruct Miscanthus cell walls, although they did perform better than the most widely used fungus Trichoderma reesei [34]. For industrial applications, fast growing organisms, as obtained here, with high-titre enzyme production are generally favourable.

The yeast from the non-selective culturing were the least likely to be producing the enzymes. For example, the Meyerozyma, which was cultured five times showed none of the four enzyme activities. However, an isolate from this species was previously shown to have exoglucanase activity, which we did not test for here [76]. In the targeted approach however, the yeast isolates mostly expressed one or two enzymes. To our knowledge, some of these species of potentially oleaginous yeasts such as those belonging to the extremophile genus Naganishia [77, 78] have never been shown to produce carbohydrate hydrolases before. An early study of microbes growing in bagasse [15] found similarities in the microbial composition of naturally stored versus fermented bagasse with a succession of organisms growing in the bagasse coinciding with their enzyme activity. Yeast first dominated as they consumed the residual sucrose, then bacteria took over and degraded hemicellulose and cellulose, finally the other fungi infiltrated, digesting the hemicellulose, cellulose and lignin. In this study, yeasts were only found in the top layers of the pile at first but some, such as Rhodoturola, progressed deeper into the pile with time and with increasing degradation. Modern mills such as the one sampled in this study do not leave as much sucrose in the bagasse as would have been the case in the early study. The yeast here are more likely to be feeding off pentose and hexose sugars released from degradation or using enzymes themselves as has also been observed in rotting wood [79].

Selection of organisms on media containing lignin as the sole carbon source was not effective in selecting microbes with lignin peroxidase activity, with only one strain out of 12 showing strong decolourisation of Azure B. However, most produced laccase, with 5 out of 12 also producing the other two enzymes (xylanase and CMCase) and another 5/12 showing two activities. Azure B decolourisation was initially shown to be specific for lignin peroxidase [41] and is not decolourised by laccase. However, we found yeast and Bacillus species with Azure B decolourisation activity, suggesting that other enzymes such as quinone dehydrogenase, as previously shown in Bacillus [42, 43], or that some other novel enzyme activity may have been involved. The fungi may also have been able to grow by consuming residual hemicellulose and/or cellulose associated with the lignin and/or by producing agarase. Indeed, many of the microbes cultivated did show some agarase activity (results not shown). Similarly, only one oxidase-producing organism, similar to Penicillium glabrum (RP93), was isolated on plates containing tannic acid because all the other colonies that grew were producing tannase (results not shown).

We confirmed the functional presence of these organisms in the bagasse pile by showing that their xylanase production was specifically induced in the presence of bagasse (Fig. 3b). The strains RP4, and RP68, similar to the uncharacterised species Talaromyces rugulosus and Coniochaeta taeniospora, respectively, showed relatively high xylanase activity, comparable to that of the well-characterised A. fumigatus [80, 81] and T. terrestris [19, 69] (Fig. 3b). Overall, the three isolated Coniochaeta species were among the best enzyme producers in this study, producing relatively high levels of all four enzymes. Recent draft genome sequences of several of these wood rot fungi have revealed their large arsenal of genes for biomass-degradation, including genes encoding novel lignin-degrading enzymes [82, 83]. The cultured strains are now a resource for mining new lignocellulosic modifying enzymes. Finally, the prevalence of biomass degrading microbes in the pile and potentially novel fungi, as shown through amplicon sequencing, suggests that bagasse piles could be a relevant environment for functional metagenomics studies to isolate novel enzymes.

Conclusions

The culture-dependent approach used here allowed novel biomass-degrading microbes to be isolated. While the glucanases and hemicellulases of many organisms similar to those found here have been well characterised, for many microorganisms the discovery of laccase and peroxidase activity is novel and provides a basis for further study. The culture-independent approach using amplicon sequencing provided data on the overall microbial biodiversity. Good consistency was observed between the fungi that were cultured and the fungi identified in the sequencing although 33% of the Ascomycota reads were similar to uncultured or unknown species. The findings from both culturing and amplicon sequencing suggest that relatives of the thermophilic biomass-degrading fungi T. terrrestris and R. emersonii were the most abundant in the deep layers of the pile and could have been significantly involved in the hemicellulose degradation observed. In terms of bacteria involved in the degradation, the Firmicutes, Bacillus and Alicyclobacillus species may have played a significant role based on their abundance in culturing and amplicon sequencing. Overall, the variety of species that were found in this study and that are known for biomass degradation shows that bagasse piles are a valuable selective environment for the identification of new microbes and enzymes with biotechnological potential.

Methods

Bagasse sampling and analysis

Bagasse was sampled from a stockpile at the Rocky Point sugarcane mill in Woongoolba (27.7413°S, 153.3148°E, 7 m AMSL), Queensland, Australia. In 2015, 2016 and 2017 the mill crushed 383,832, 110,231 (smaller than usual amount due to technical difficulties) and 388,484 t, respectively, in the crushing season from July to December [19, 20]. The area has a humid subtropical climate with an average maximum temperature of 26 °C, minimum of 15 °C and an average rainfall of 1087 mm [21] mostly falling from November to March.

Bagasse samples were taken from a stockpile (excess bagasse once most of it was used to run the furnace) at the Rocky Point mill. It is a relatively small pile compared to industry standards, approximately 5 m high and 20 m across. On the 31st May 2016, approximately 6 months since the end of crushing the previous year, samples were taken at three places in the pile: the top, approximately 10 cm under the crust, and approximately 50 cm into the pile. On the 22nd February 2017, further samples were taken on the surface, just below the surface, approximately 30 cm deep and approximately 80 cm deep (Table 1).

For sampling, bagasse was scooped into sterile 50 mL tubes without touching it. Larger samples for analysis were taken with plastic bags. Samples were stored on ice while transported back to the lab. Samples for metagenomics were then stored at − 40 °C, while samples for culturing and analysis were stored at 4 °C and used within days.

The temperature was measured in situ using an Infrared Non-Contact Digital Thermometer. The pH was measured on fresh bagasse using one-part bagasse to 2.5 parts water with a pH meter. The moisture content was determined by oven drying and weighing starting with three replicates of approximately 50 g (wet weight) of sample. For compositional analysis, the bagasse was dried at 40 °C for 3 days and then ground to a fine powder with a Retsch SM 100 cutting mill. To determine the proportions of structural carbohydrates and lignin in the different bagasse samples, 300 mg +/− 10 mg was analysed according to Sluiter et al. [22]. A reference bagasse sample was analysed at the same time. A one-way analysis of variance (ANOVA) was performed on the data using SigmaPlot (version 13.0) to check for significant differences between the samples at p < 0.05. All data passed Normality (Shapiro-Wilk) and Equal Variance tests (Brown-Forsythe) tests. Pairwise multiple comparisons were carried you with the Holm-Sidak method. Degrees of freedom was 14.

Media and plate enzyme assays

Microbes were routinely cultivated on Yeast Peptone Dextrose media (YPD: 2% bacteriological peptone (Oxoid, Thermo Fisher Scientific, Australia); 1% yeast extract (Sigma-Aldrich, Australia); 2% D-glucose (Sigma-Aldrich, Australia); 2% bacteriological agar (Oxoid, Thermo Fisher Scientific, Australia)), Nutrient Agar (Oxoid, Thermo Fisher Scientific, Australia) or Potato Dextrose Agar (PDA; Oxoid, Thermo Fisher Scientific, Australia). Chloramphenicol (Sigma-Aldrich, Australia) was added to plates at 25 mg/mL. For enzyme assays, 1 x (0.68%) Yeast Nitrogen base with amino acids (Sigma-Aldrich, Australia) was used as the base media to which various substrates were added: 0.02% azo-xylan (Azo-wheat arabinoxylan (1% w/v unbuffered) (Megazyme, Australia); 0.5% carboxy-methylcellulose (CMC (Sigma-Aldrich, Australia) with 0.01% Trypan Blue (Sigma-Aldrich, Australia); 0.05% remazol brilliant blue (Sigma-Aldrich, Australia); 0.01% azure B (Sigma-Aldrich, Australia); 0.02% (1.8 mM) Guiaicol (Sigma-Aldrich, Australia); 0.5% lignin (prepared ‘in house’); 2% finely ground bagasse with or without 2% glucose and with 2% bacteriological agar. For xylanase assays, media was made with 1x YNB and 2% finally ground bagasse with or without glucose.

To observe whether microbes in the collection have cellulase (endoglucanase-CMCase), xylanase, laccase or lignin peroxidase activity, they were grown on media containing substrate and/or dyes described in media section. These substrates were validated using purified enzymes (e.g. Accellerase 1500 (Genencor)) and /or organisms known to show the activity (for e.g. Botrytis for laccase) when possible. Yeast or bacteria were patched in a quarter or third of a plate whereas fungi were patched in three replicates on a single plate to avoid cross-contamination. The plates were incubated for 7 days at 28 °C or 50 °C and clearing zones and growth observed. No attempt was made to quantify the activity, a positive activity was noted for any clearance zone produced within the 7 days. However, if it took longer than 7 days for the clearance zone to appear, the activity was noted as low.

Xylanase assay of microbes growing in bagasse containing media

The production of xylose, cleaved from beechwood xylan, was quantified using the dinitrosalicylic acid reducing sugar (DNS) assay [23], adapted for microplates. 50 μL of diluted enzyme in DNS assay buffer (100 mM Acetate buffer pH 5.0, 20 mM CaCl2, 0.01% Tween 20) was pipetted into 200 μL wells of a 96-well PCR plate containing 125 μL of 0.4% (weight/volume) beechwood xylan, mixed and incubated for 30 min at 37 °C. After incubation, 75 μL of DNS stop reagent was added to each sample and samples were heated to 100 °C for 5 min before the absorbance at 530 nm was measured. Each enzyme dilution was quantified in triplicate. For blanks, the substrate and DNS stop reagent were mixed first, followed by the addition of the dilute enzyme sample. One unit of enzyme activity was defined as the amount of sample that released 1 μmol of reducing sugar equivalents from xylan substrate per minute under the assay conditions used.

Culturing and identification

To culture microbes (bacteria and fungi) from the bagasse, approximately 1 g of bagasse was ground in 10 mL of NaCl/ Tween80 (0.9%/0.01%) and then mixed for 30 min. Serial dilutions were then plated out and incubated at 28 °C or 50 °C. Distinct morphotypes were selected and restreaked to obtain pure cultures which were then stored as glycerol stocks (30% glycerol for yeast or bacteria or 12.4% glycerol plus 0.04% tween-80 for fungal spores). Microbes were identified by sequencing barcode regions. For this, DNA was extracted using the Lithium Acetate/SDS protocol of Looke et al. [24]. Then 1 μL was used to amplify the full length 16S, or partial 18S or ITS sequence from bacteria, yeast or filamentous fungi respectively using the primers shown in Table 2.

Table 2 primers used for rRNA amplification and sequencing
Full size table

PCR was carried out using OneTaq (NEB) following the manufacturer’s instructions and the annealing temperature shown in Table 2 for 35 cycles. Products were sequenced at the Central Analytical Research Facility at the Queensland University of Technology. Sequences were used to query the 16S ribosomal sequence (bacterial and archeae) database at NCBI or the UNITE [35] database, respectively. The strains were identified to the species level (when possible) based on the top BLAST hit (e-values) and % identity.

Amplicon sequencing

Microbial genomic DNA was extracted from approximately 250 mg of bagasse using the DNAeasy Powersoil Kit including PowerBead tubes (Qiagen Australia) following the manufacturer’s instructions. For sample 1 (50 cm from May), more than one extraction was carried out and pooled and precipitated in an attempt to obtain sufficient DNA. PCRs were then performed on 5–10 ng of this gDNA using primers targeting the V3 and V4 region [87] or the ITS2 region for fungi [26]. Illumina adapter overhang nucleotide sequences were added to the gene-specific sequences. The full-length primer sequences (using standard IUPAC nucleotide nomenclature) were:

16S Amplicon PCR Forward Primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3′

16S Amplicon PCR Reverse Primer -

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′ ITS3_KYO2 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGATGAAGAACGYAGYRAA-3′

ITS4_KYO3 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCTBTTVCCKCTTCACTCG-3′

Amplifications were carried out with Q5 polymerase (New England Biolabs, Genesearch Australia) following the manufacturer’s instructions using 1 μM primer and at five different annealing temperatures: 53 °C, 55 °C, 58 °C, 60 °C, 63 °C for 16S and 54 °C, 58 °C, 60 °C, 62.5 °C and 65 °C for ITS for 25 cycles. Large primer dimers (~ 150 bp) obtained with the ITS primers were removed by cutting pooled PCR fragments out of a gel and purifying the DNA with ISOLATE II PCR and Gel Kit (Bioline, Meridian Bioscience, Australia) following the manufacturer’s instructions. Amplicons were prepared for Illumina sequencing using the MiSeq Reagent Nano Kit v2 (Illumina Australia) and the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s instructions. Pooled indexed libraries were then sequenced on an Illumina MiSeq to yield 300 bp paired end sequences (version 3 technology).

Metabarcoding sequencing analysis

Amplicon sequences were processed and assigned to operational taxonomic units (OTUs) using the MICrobial Community Analysis (Micca) pipeline [88] (version 1.6.2) using the docker image and following the paired-end sequencing tutorial: https://micca.readthedocs.io/en/latest/pairedend_97.html. Briefly, paired end reads were merged, trimmed to remove primer sequences and then quality filtered. To characterize the taxonomic structure of the samples, the sequences were then organized into Operational Taxonomic Units (OTUs) at 97% identity using the ‘otu’ command which implements de novo greedy clustering. OTUs were then classified using the Ribosomal Database Project II classifier (version 11.5) for 16S sequences [28] and the UNITE database (version 7.2) for ITS sequences [29]. Finally, Micca was used to summarize and rarefy the data to compare the taxonomic composition of each sample. Sampling heterogeneity was reduced by rarefaction to a read depth of 45,000 and 41,000 for 16S and ITS, respectively. The full list of commands used are shown in Additional file 6.

Availability of data and materials

The datasets generated and/or analysed during the current study are available as follows:

The strains are described in Additional file 1 and are stored at QUT at the authors’ address.

Partial ribosomal RNA sequences for the three Coniochaeta isolates were submitted to NCBI under the following accession numbers: MN216224 (RP12), MN218196 (RP62), MN218197 (RP68).

The 16S and ITS reads were deposited at the NCBI short read archive under BioProject ID: PRJNA530327 https://www.ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA530327

Taxonomic classification of the amplicon sequencing data is provided in Additional files 2, 3, 4 and 5.

Abbreviations

CMC:

Carboxy-methyl cellulose

DNS:

Dinitrosalicylic acid reducing sugar

gDNA:

Genomic DNA

ITS:

Internal transcriber sequence

NCBI:

The National Centre for Biotechnology Information

OTU:

Operational Taxonomic Units

PCR:

Polymerase chain reaction

References

  1. Sugar. World Markets and Trade. Washington, DC: 20250: United States Department of Agriculture; 2018.

    Google Scholar 

  2. Birru E, Erlich C, Martin A. Energy performance comparisons and enhancements in the sugar cane industry. Biomass Conversion Biorefinery. 2019;9(2):267–82.

    Article  Google Scholar 

  3. Zhang ZY, Rackemann DW, Doherty WOS, O’Hara IM. Glycerol carbonate as green solvent for pretreatment of sugarcane bagasse. Biotechnol Biofuels. 2013;6:153.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Sharma RK, Yadav KR, Maheshwari VL, Kothari RM. Baggasse preservation: a need for a biotechnological approach. Crit Rev Biotechnol. 2000;20(4):237–63.

    Article  CAS  PubMed  Google Scholar 

  5. Chinga-Carrasco G, Ehman NV, Pettersson J, Vallejos ME, Brodin MW, Felissia FE, et al. Pulping and pretreatment affect the characteristics of bagasse inks for three-dimensional printing. ACS Sustain Chem Eng. 2018;6(3):4068–75.

    Article  CAS  Google Scholar 

  6. Bian J, Peng F, Peng X-P, Peng P, Xu F, Sun R-C. Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse. Bioresour Technol. 2013;127:236–41.

    Article  CAS  PubMed  Google Scholar 

  7. Silva CF, Azevedo RS, Braga C, da Silva R, Dias ES, Schwan RF. Microbial diversity in a bagasse-based compost prepared for the production of Agaricus brasiliensis. Braz J Microbiol. 2009;40(3):590–600.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Thomas L, Larroche C, Pandey A. Current developments in solid-state fermentation. Biochem Eng J. 2013;81:146–61.

    Article  CAS  Google Scholar 

  9. Pandey A, Soccol CR, Nigam P, Soccol VT. Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresour Technol. 2000;74(1):69–80.

    Article  CAS  Google Scholar 

  10. Schutyser W, Renders T, Van den Bosch S, Koelewijn SF, Beckham GT, Sels BF. Chemicals from lignin: an interplay of lignocellulose fractionation, depolymerisation, and upgrading. Chem Soc Rev. 2018;47(3):852–908.

    Article  CAS  PubMed  Google Scholar 

  11. Harrison MD. Sugarcane-derived animal feed. In: O’Hara IM, Sagadevan M, editors. Sugarcane-based biofuels and bioproducts. Hoboken: Wiley; 2016. p. 281–300.

    Google Scholar 

  12. Lacey J. Molding of Sugar-Cane Bagasse and Its Prevention. Ann Appl Biol. 1974;76(1):63.

    Article  CAS  Google Scholar 

  13. Mhuantong W, Charoensawan V, Kanokratana P, Tangphatsornruang S, Champreda V. Comparative analysis of sugarcane bagasse metagenome reveals unique and conserved biomass-degrading enzymes among lignocellulolytic microbial communities. Biotechnol Biofuels. 2015;8:16.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Rattanachomsri U, Kanokratana P, Eurwilaichitr L, Igarashi Y, Champreda V. Culture-independent phylogenetic analysis of the microbial Community in Industrial Sugarcane Bagasse Feedstock Piles. Biosci Biotechnol Biochem. 2011;75(2):232–9.

    Article  CAS  PubMed  Google Scholar 

  15. Schmidt O, Walter K. Succession and activity of microorganisms in stored bagasse. Eur J Appl Microbiol. 1978;5(1):69–77.

    Article  CAS  Google Scholar 

  16. Wright M, Lima I, Bigner R. Microbial and physicochemical properties of sugarcane bagasse for potential conversion to value-added products. Int Sugar J. 2016;118:10–8.

    Google Scholar 

  17. BSLd S, AFS G, Franciscon EG, JMd O, Baffi MA. Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production. Braz J Microbiol. 2015;46:903–10.

    Article  CAS  Google Scholar 

  18. Batista-García RA, Balcázar-López E, Miranda-Miranda E, Sánchez-Reyes A, Cuervo-Soto L, Aceves-Zamudio D, et al. Characterization of Lignocellulolytic activities from a moderate halophile strain of Aspergillus caesiellus isolated from a sugarcane bagasse fermentation. PLoS One. 2014;9(8):e105893.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Garcia-Huante Y, Cayetano-Cruz M, Santiago-Hernandez A, Cano-Ramirez C, Marsch-Moreno R, Campos JE, et al. The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable beta-1,4-xylanase with exo- and endo-activity. Extremophiles. 2017;21(1):175–86.

    Article  CAS  PubMed  Google Scholar 

  20. Moretti MMS, Bocchini-Martins DA, Silva RD, Rodrigues A, Sette LD, Gomes E. Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. Braz J Microbiol. 2012;43(3):1062–71.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Shrestha P, Szaro TM, Bruns TD, Taylor JW. Systematic search for cultivatable Fungi that best deconstruct cell walls of Miscanthus and sugarcane in the Field. Appl Environ Microb. 2011;77(15):5490–504.

    Article  CAS  Google Scholar 

  22. Kanokratana P, Mhuantong W, Laothanachareon T, Tangphatsornruang S, Eurwilaichitr L, Pootanakit K, et al. Phylogenetic analysis and metabolic potential of microbial communities in an industrial bagasse collection site. Microb Ecol. 2013;66(2):322–34.

    Article  CAS  PubMed  Google Scholar 

  23. Kanokratana P, Eurwilaichitr L, Pootanakit K, Champreda V. Identification of glycosyl hydrolases from a metagenomic library of microflora in sugarcane bagasse collection site and their cooperative action on cellulose degradation. J Biosci Bioeng. 2015;119(4):384–91.

    Article  CAS  PubMed  Google Scholar 

  24. Batista García R, Casasanero R, Alvarez-Castillo A, Dobson A, Folch-Mallol J. Prokaryotic diversity from the culture-independent taxonomic analysis of a sugarcane bagasse metagenome. Merit Res J Microbiol Biol Sci. 2016;4:022–38.

    Google Scholar 

  25. Wongwilaiwalin S, Laothanachareon T, Mhuantong W, Tangphatsornruang S, Eurwilaichitr L, Igarashi Y, et al. Comparative metagenomic analysis of microcosm structures and lignocellulolytic enzyme systems of symbiotic biomass-degrading consortia. Appl Microbiol Biot. 2013;97(20):8941–54.

    Article  CAS  Google Scholar 

  26. Alvarez TM, Paiva JH, Ruiz DM, Cairo JP, Pereira IO, Paixao DA, et al. Structure and function of a novel cellulase 5 from sugarcane soil metagenome. PLoS One. 2013;8(12):e83635.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  27. Alvarez TM, Goldbeck R, dos Santos CR, Paixao DA, Goncalves TA, Franco Cairo JP, et al. Development and biotechnological application of a novel endoxylanase family GH10 identified from sugarcane soil metagenome. PLoS One. 2013;8(7):e70014.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Heiss-Blanquet S, Fayolle-Guichard F, Lombard V, Hebert A, Coutinho PM, Groppi A, et al. Composting-like conditions are more efficient for enrichment and diversity of organisms containing Cellulase-encoding genes than submerged cultures. PLoS One. 2016;11(12):e0167216.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Mello BL, Alessi AM, McQueen-Mason S, Bruce NC, Polikarpov I. Nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia. Sci Rep. 2016;6:38781.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  30. Ventorino V, Aliberti A, Faraco V, Robertiello A, Giacobbe S, Ercolini D, et al. Exploring the microbiota dynamics related to vegetable biomasses degradation and study of lignocellulose-degrading bacteria for industrial biotechnological application. Sci Rep. 2015;5:8161.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  31. Ventorino V, Ionata E, Birolo L, Montella S, Marcolongo L, de Chiaro A, et al. Lignocellulose-adapted Endo-Cellulase producing Streptomyces strains for bioconversion of cellulose-based materials. Front Microbiol. 2016;7:2061.

    Article  PubMed Central  PubMed  Google Scholar 

  32. Lagier J-C, Dubourg G, Million M, Cadoret F, Bilen M, Fenollar F, et al. Culturing the human microbiota and culturomics. Nat Rev Microbiol. 2018;16(9):540–50.

    Article  CAS  PubMed  Google Scholar 

  33. Batista-Garcia RA, Sanchez-Carbente MD, Talia P, Jackson SA, O'Leary ND, Dobson ADW, et al. From lignocellulosic metagenomes to lignocellulolytic genes: trends, challenges and future prospects. Biofuels Bioprod Biorefin. 2016;10(6):864–82.

    Article  CAS  Google Scholar 

  34. Chase AB, Karaoz U, Brodie EL, Gomez-Lunar Z, Martiny AC, Martiny JBH. Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits. Mbio. 2017;8:e01809–17.

  35. Nilsson RH, Larsson KH, Taylor AFS, Bengtsson-Palme J, Jeppesen TS, Schigel D, et al. The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Res. 2019;47(D1):D259–D264.

    Article  PubMed Central  CAS  Google Scholar 

  36. Yaegashi J, Kirby J, Ito M, Sun J, Dutta T, Mirsiaghi M, et al. Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts. Biotechnol Biofuels. 2017;10:241.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  37. Zhu ZW, Zhang SF, Liu HW, Shen HW, Lin XP, Yang F, et al. A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides. Nat Commun. 2012;3:1112.

    Article  CAS  PubMed  Google Scholar 

  38. Castanha RF, Mariano AP, de Morais LAS, Scramin S, Monteiro RTR. Optimization of lipids production by Cryptococcus laurentii 11 using cheese whey with molasses. Braz J Microbiol. 2014;45(2):379–87.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  39. Ramírez-Castrillón M, Jaramillo-Garcia VP, Rosa PD, Landell MF, Vu D, Fabricio MF, et al. The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization. Frontiers Microbiol. 2017;8:1776.

    Article  Google Scholar 

  40. Abrao FO, Duarte ER, Pessoa MS, Santos VLD, Freitas Junior LF, Barros KO, et al. Notable fibrolytic enzyme production by Aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignified pastures. Plos One. 2017;12(8):e0183628.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  41. Lim Y, Kim K, Park U. Isolation of Lecythophora sp. YP363, a secretor of various thermostable plant cell wall-degrading enzymes with high activity. Afr J Microbiol Res. 2013;7(27):3517–25.

    Google Scholar 

  42. Petrosyan P, García-Varela M, Luz-Madrigal A, Huitrón C, Flores ME. Streptomyces mexicanus sp. nov., a xylanolytic micro-organism isolated from soil. Int J Syst Evol Microbiol. 2003;53(1):269–73.

    Article  CAS  PubMed  Google Scholar 

  43. Yilmaz P, Kottmann R, Field D, Knight R, Cole JR, Amaral-Zettler L, et al. Minimum information about a marker gene sequence (MIMARKS) and minimum information about any (x) sequence (MIxS) specifications. Nat Biotechnol. 2011;29:415.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  44. Lee BD, Apel WA, Sheridan PP, DeVeaux LC. Glycoside hydrolase gene transcription by Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and monosaccharides: a proposed xylan hydrolysis mechanism. Biotechnol Biofuels. 2018;11:110.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  45. Yang W, Bai Y, Yang P, Luo H, Huang H, Meng K, et al. A novel bifunctional GH51 exo-α-l-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp. A4 with significant biomass-degrading capacity. Biotechnol Biofuels. 2015;8(1):197.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  46. Lee BD, Apel WA, DeVeaux LC, Sheridan PP. Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius. J Ind Microbiol Biotechnol. 2017;44(10):1443–58.

    Article  CAS  PubMed  Google Scholar 

  47. Goto K, Mochida K, Kato Y, Asahara M, Fujita R, An S-Y, et al. Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov. Int J Syst Evol Microbiol. 2007;57(6):1276–85.

    Article  CAS  PubMed  Google Scholar 

  48. Mavromatis K, Sikorski J, Lapidus A, Glavina Del Rio T, Copeland A, Tice H, et al. Complete genome sequence of Alicyclobacillus acidocaldarius type strain (104-IA). Stand Genomic Sci. 2010;2(1):9–18.

    Article  PubMed Central  PubMed  Google Scholar 

  49. Myers MR, King GM. Isolation and characterization of Acidobacterium ailaaui sp. nov., a novel member of Acidobacteria subdivision 1, from a geothermally heated Hawaiian microbial mat. Int J Syst Evol Microbiol. 2016;66(12):5328–35.

    Article  CAS  PubMed  Google Scholar 

  50. Yabe S, Aiba Y, Sakai Y, Hazaka M, Yokota A. Thermosporothrix hazakensis gen. Nov., sp. nov., isolated from compost, description of Thermosporotrichaceae fam. Nov. within the class Ktedonobacteria Cavaletti et al. 2007 and emended description of the class Ktedonobacteria. Int J Syst Evol Microbiol. 2010;60(8):1794–801.

    Article  CAS  PubMed  Google Scholar 

  51. Yabe S, Sakai Y, Yokota A. Thermosporothrix narukonensis sp. nov., belonging to the class Ktedonobacteria, isolated from fallen leaves on geothermal soil, and emended description of the genus Thermosporothrix. Int J Syst Evol Microbiol. 2016;66(6):2152–7.

    Article  CAS  PubMed  Google Scholar 

  52. Davis JR, Goodwin LA, Woyke T, Teshima H, Bruce D, Detter C, et al. Genome sequence of Amycolatopsis sp. strain ATCC 39116, a plant biomass-degrading actinomycete. J Bacteriol. 2012;194(9):2396–7.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  53. Houbraken J, Spierenburg H, Frisvad JC. Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie Van Leeuwenhoek. 2012;101(2):403–21.

    Article  CAS  PubMed  Google Scholar 

  54. Martínez PM, Appeldoorn MM, Gruppen H, Kabel MA. The two Rasamsonia emersonii α-glucuronidases, ReGH67 and ReGH115, show a different mode-of-action towards glucuronoxylan and glucuronoxylo-oligosaccharides. Biotechnol Biofuels. 2016;9(1):105.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  55. Tuohy MG, Coughlan MP. Production of thermostable xylan-degrading enzymes by Talaromyces emersonii. Bioresour Technol. 1992;39(2):131–7.

    Article  CAS  Google Scholar 

  56. Wang K, Luo H, Bai Y, Shi P, Huang H, Xue X, et al. A thermophilic endo-1,4-β-glucanase from Talaromyces emersonii CBS394.64 with broad substrate specificity and great application potentials. Appl Microbiol Biot. 2014;98(16):7051–60.

    Article  CAS  Google Scholar 

  57. Yilmaz N, Visagie CM, Houbraken J, Frisvad JC, Samson RA. Polyphasic taxonomy of the genus Talaromyces. Stud Mycol. 2014;78:175–341.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  58. He R, Bai X, Cai P, Sun C, Zhang D, Chen S. Genome sequence of Talaromyces piceus 9–3 provides insights into lignocellulose degradation. 3 Biotech. 2017;7(6):368.

    Article  PubMed Central  PubMed  Google Scholar 

  59. de Eugenio LI, Méndez-Líter JA, Nieto-Domínguez M, Alonso L, Gil-Muñoz J, Barriuso J, et al. Differential β-glucosidase expression as a function of carbon source availability in Talaromyces amestolkiae: a genomic and proteomic approach. Biotechnol Biofuels. 2017;10:161.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  60. Nieto-Dominguez M, de Eugenio LI, Barriuso J, Prieto A, Fernandez de Toro B, Canales-Mayordomo A, et al. Novel pH-stable glycoside hydrolase family 3 beta-Xylosidase from Talaromyces amestolkiae: an enzyme displaying Regioselective Transxylosylation. Appl Environ Microbiol. 2015;81(18):6380–92.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  61. Guais O, Borderies G, Pichereaux C, Maestracci M, Neugnot V, Rossignol M, et al. Proteomics analysis of “Rovabio™ excel”, a secreted protein cocktail from the filamentous fungus Penicillium funiculosum grown under industrial process fermentation. J Ind Microbiol Biotechnol. 2008;35(12):1659–68.

    Article  CAS  PubMed  Google Scholar 

  62. Chi ZM, Wang F, Chi Z, Yue LX, Liu GL, Zhang T. Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Appl Microbiol Biot. 2009;82(5):793–804.

    Article  CAS  Google Scholar 

  63. Gostincar C, Ohm RA, Kogej T, Sonjak S, Turk M, Zajc J, et al. Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species. BMC Genomics. 2014;15:549.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  64. Kudanga T, Mwenje E. Extracellular cellulase production by tropical isolates of Aureobasidium pullulans. Can J Microbiol. 2005;51(9):773–6.

    Article  CAS  PubMed  Google Scholar 

  65. McHunu NP, Permaul K, Abdul Rahman AY, Saito JA, Singh S, Alam M. Xylanase Superproducer: genome sequence of a compost-loving Thermophilic fungus, Thermomyces lanuginosus strain SSBP. Genome Announc . 2013;1(3):e00388–13.

    Article  PubMed Central  PubMed  Google Scholar 

  66. Martinez D, Larrondo LF, Putnam N, Gelpke MDS, Huang K, Chapman J, et al. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nat Biotechnol. 2004;22:695.

    Article  CAS  PubMed  Google Scholar 

  67. Hassanpour M, Cai G, Gebbie LK, Speight RE, Junior Te'o VS, O'Hara IM, et al. Co-utilization of acidified glycerol pretreated-sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain. Engineering Life Sci. 2019;19(3):217–28.

    Article  CAS  Google Scholar 

  68. Sarkar S, Chakravorty S, Mukherjee A, Bhattacharya D, Bhattacharya S, Gachhui R. De novo RNA-Seq based transcriptome analysis of Papiliotrema laurentii strain RY1 under nitrogen starvation. Gene. 2018;645:146–56.

    Article  CAS  PubMed  Google Scholar 

  69. Berka RM, Grigoriev IV, Otillar R, Salamov A, Grimwood J, Reid I, et al. Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris. Nat Biotechnol. 2011;29(10):922–U222.

    Article  CAS  PubMed  Google Scholar 

  70. Sharmin F, Wakelin S, Huygens F, Hargreaves M. Firmicutes dominate the bacterial taxa within sugar-cane processing plants. Sci Rep. 2013;3:3107.

    Article  PubMed Central  PubMed  Google Scholar 

  71. Xu J. Fungal DNA barcoding. Genome. 2016;59(11):913–32.

    Article  CAS  PubMed  Google Scholar 

  72. Toju H, Tanabe AS, Yamamoto S, Sato H. High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples. Plos One. 2012;7(7):e40863.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  73. Xu XM, Passey T, Wei F, Saville R, Harrison RJ. Amplicon-based metagenomics identified candidate organisms in soils that caused yield decline in strawberry. Hortic Res-England. 2015;2:15022.

    Article  CAS  Google Scholar 

  74. Asemaninejad A, Weerasuriya N, Gloor GB, Lindo Z, Thorn RG. New primers for discovering fungal diversity using nuclear large ribosomal DNA. PLoS One. 2016;11(7):e0159043.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  75. Zhang L, Ma H, Zhang H, Xun L, Chen G, Wang L. Thermomyces lanuginosus is the dominant fungus in maize straw composts. Bioresour Technol. 2015;197:266–75.

    Article  CAS  PubMed  Google Scholar 

  76. Kuo H-W, Zeng J-K, Wang P-H, Chen W-C. A novel Exo-Glucanase explored from a Meyerozyma sp. Fungal Strain. Adv Enzyme Res. 2015;3(3):53–65.

    Article  CAS  Google Scholar 

  77. Schmidt SK, Vimercati L, Darcy JL, Arán P, Gendron EMS, Solon AJ, et al. A Naganishia in high places: functioning populations or dormant cells from the atmosphere? Mycology. 2017;8(3):153–63.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  78. Selvakumar P, Sivashanmugam P. Study on lipid accumulation in novel oleaginous yeast Naganishia liquefaciens NITTS2 utilizing pre-digested municipal waste activated sludge: a low-cost feedstock for biodiesel production. Appl Biochem Biotechnol. 2018;186(3):731–49.

    Article  CAS  PubMed  Google Scholar 

  79. Cadete RM, Lopes MR, Rosa CA. Yeasts associated with decomposing plant material and rotting wood. In: Buzzini P, Lachance M-A, Yurkov A, editors. Yeasts in natural ecosystems: diversity. Cham: Springer International Publishing; 2017. p. 265–92.

    Chapter  Google Scholar 

  80. Carvalho AFA, PdO N, Zaghetto de Almeida P, Bueno da Silva J, Escaramboni B, Pastore GM. Screening of Xylanolytic Aspergillus fumigatus for prebiotic Xylooligosaccharide production using bagasse. Food Technol Biotechnol. 2015;53(4):428–35.

    CAS  PubMed Central  PubMed  Google Scholar 

  81. Liu D, Li J, Zhao S, Zhang R, Wang M, Miao Y, et al. Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatusZ5 in the presence of different carbon sources. Biotechnol Biofuels. 2013;6(1):149.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  82. Borstlap CJ, de Witt RN, Botha A, Volschenk H. Draft genome sequence of the lignocellulose-degrading Ascomycete Coniochaeta pulveracea CAB 683. Microbiol Res Announc. 2019;8(1):e01429–18.

    Google Scholar 

  83. Jiménez DJ, Hector RE, Riley R, Lipzen A, Kuo RC, Amirebrahimi M, et al. Draft genome sequence of Coniochaeta ligniaria NRRL 30616, a Lignocellulolytic fungus for bioabatement of inhibitors in plant biomass Hydrolysates. Genome Announc. 2017;5(4):e01476–16.

    Article  PubMed Central  PubMed  Google Scholar 

  84. Lane DJ. 16S/23S rRNA Sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques in bacterial systematic. New York: Wiley; 1991. p. 115–75.

    Google Scholar 

  85. Bellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, Kauserud H. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. BMC Microbiol. 2010;10(1):189.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  86. Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol. 1997;35(5):1216–23.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  87. Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 2013;41(1):e1.

    Article  CAS  PubMed  Google Scholar 

  88. Albanese D, Fontana P, De Filippo C, Cavalieri D, Donati C. MICCA: a complete and accurate software for taxonomic profiling of metagenomic data. Sci Rep. 2015;5:9743.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgements

We would like to thank Bruce Tyson, Peter Bressow and the Heck group from Rocky Point Mill for allowing us access to the bagasse pile. The data reported in this paper were obtained at the Central Analytical Research Facility (CARF) operated by the Institute for Future Environments (QUT). Access to CARF is supported by generous funding from the Science and Engineering Faculty (QUT). We would like to thank Vincent Chand, Kevin Dudley and Victoria Coyne from CARF for their excellent support and technical assistance. Many thanks to James Strong for useful discussions.

Funding

This project was supported by Sugar Research Australia, through funding from the Australian Government Department of Agriculture as part of its Rural R&D for Profit programme, the Queensland Government and project partners including Cotton Research and Development Corporation, Forest & Wood Products Australia, Australian Pork Ltd., Southern Oil Refining, NSW Department of Primary Industries and Queensland University of Technology. The funding bodies did not participate in the design of the study nor the collection, analysis, and interpretation of data or writing of the manuscript.

Author information

Authors and Affiliations

  1. Queensland University of Technology, 2 George St, Brisbane, QLD, 4000, Australia

    Leigh Gebbie, Tuan Tu Dam, Rebecca Ainscough, Li Cao, Mark Harrison, Ian O’Hara & Robert Speight

  2. Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St. Lucia, QLD, 4072, Australia

    Robin Palfreyman

Authors
  1. Leigh Gebbie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tuan Tu Dam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rebecca Ainscough
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robin Palfreyman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mark Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ian O’Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Robert Speight
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

LG designed the study, carried out experiments and wrote the manuscript. TTD carried out significant sections of the experimental work. RA and LC provided experimental support. RP carried out bioinformatics analyses. MH and IO contributed to study design, assisted with data interpretation and analysis, and edited the manuscript. RS designed the study, interpreted data and wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Robert Speight.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Additional file 1.

List of the strains isolated from bagasse including a detailed description of isolation conditions and homology of rRNA sequences.

Additional file 2.

List of unique 16S amplicons assigned to individual OTUs, their taxonomic classification to the genus level and the number of times these were amplified from each sample (after rarefaction to 45,000 to reduce sample heterogeneity).

Additional file 3.

List of unique ITS amplicons assigned to individual OTUs, their taxonomic classification to the species level and the number of times these were amplified from each sample (after rarefaction to 41,000 to reduce sample heterogeneity).

Additional file 4.

Taxonomic classification of 16S OTUs at six levels (kingdom to genus) and their abundance in each sample (after rarefaction).

Additional file 5.

Taxonomic classification of 16S OTUs at seven levels (kingdom to species) and their abundance in each sample (after rarefaction).

Additional file 6.

List of commands used to run Micca for 16S and ITS analysis.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gebbie, L., Dam, T.T., Ainscough, R. et al. A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile. BMC Biotechnol 20, 12 (2020). https://doi.org/10.1186/s12896-020-00609-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12896-020-00609-y

Keywords

  • Sugarcane
  • Bagasse
  • Lignocellulose
  • Biomass
  • Cellulase
  • Xylanase
  • Laccase
  • Peroxidase
  • Amplicon sequencing
  • Fungi

BMC Biotechnology

ISSN: 1472-6750

Contact us