Table 2 primers used for rRNA amplification and sequencing
From: A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile
Microbe | Primer sequencing | References | OneTaq annealing temp (°C) | Approximate Product size (bp) |
|---|---|---|---|---|
Bacteria | 27F 5′-AGAGTTTGATCCTGGCTCAG-3′ 1492R 5′-ACGGCTACCTTGTTACGACT-3’ | [84] | 52 | 1.5 kb |
Fungi (including yeast) | ITS1F 5’-TCCGTAGGTGAACCTGCGG-3′ ITS4R 5′-TCCTCCGCTTATTGATATGC-3’ | [85] | 49 | 600 bp |
Fungi (but not yeast) | SCTB6-F 5’-GCATATCAATAAGCGGAGG-3′ LR3-R 5′-CCGTGTTTCAAGACGGG-3’ | [21] | 48 | 550 bp |
yeast | NL1F 5’-GCATATCAATAAGCGGAGGAAAAG-3′ NL4R 5′-GGTCCGTGTTTCAAGACGG-3’ | [86] | 51 | 550 bp |