An intein with genetically selectable markers provides a new approach to internally label proteins with GFP
© Ramsden et al; licensee BioMed Central Ltd. 2011
Received: 7 March 2011
Accepted: 27 June 2011
Published: 27 June 2011
Inteins are proteins that catalyze their own removal from within larger precursor proteins. In the process they splice the flanking protein sequences, termed the N-and C-terminal exteins. Large inteins frequently have a homing endonuclease that is involved in maintaining the intein in the host. Splicing and nuclease activity are independent and distinct domains in the folded structure. We show here that other biochemical activities can be incorporated into an intein in place of the endonuclease without affecting splicing and that these activities can provide genetic selection for the intein. We have coupled such a genetically marked intein with GFP as the N-terminal extein to create a cassette to introduce GFP within the interior of a targeted protein.
The Pch PRP8 mini-intein of Penicillium chrysogenum was modified to include: 1) aminoglycoside phosphotransferase; 2) imidazoleglycerol-phosphate dehydratase, His5 from S. pombe ; 3) hygromycin B phosphotransferase; and 4) the transcriptional activator LexA-VP16. The proteins were inserted at the site of the lost endonuclease. When expressed in E. coli, all of the modified inteins spliced at high efficiency. Splicing efficiency was also greater than 96% when expressed from a plasmid in S. cerevisiae. In addition the inteins conferred either G418 or hygromycin resistance, or histidine or leucine prototropy, depending on the inserted marker and the yeast genetic background. DNA encoding the marked inteins coupled to GFP as the N-terminal extein was PCR amplified with ends homologous to an internal site in the yeast calmodulin gene CMD1. The DNA was transformed into yeast and integrants obtained by direct selection for the intein's marker. The His5-marked intein yielded a fully functional calmodulin that was tagged with GFP within its central linker.
Inteins continue to show their flexibility as tools in molecular biology. The Pch PRP8 intein can successfully tolerate a variety of genetic markers and still retain high splicing efficiency. We have shown that a genetically marked intein can be used to insert GFP in one-step within a target protein in vivo.
Inteins are small proteins naturally found either embedded within a larger precursor protein (cis-acting inteins), or split between two pro-proteins (trans-acting inteins). They have the unique ability to self-catalyze their excision from the pro-protein(s) and in the process form a peptide bond between the flanking amino acid sequences that reside at the N-and C-terminal boundaries with the intein [1, 2]. These flanking regions adjacent to the intein are referred to as exteins, analogous to the terminology for introns and exons. An intein acts autonomously, splicing together the exteins without the need of a co-factor or an assisting complex. Since splicing is post-translational, for cis-acting inteins a single open reading frame driven by a single promoter expresses two distinct mature protein products: the spliced exteins and the intein.
The first intein described, Sce VMA1, was found in a subunit of the vacuolar H+-adenosine triphosphatase complex of the budding yeast Saccharomyces cerevisiae [3, 4]. Currently over 500 inteins are known, found in over 200 species of microorganisms in over 70 different proteins . Present in all three phylogenetic domains of life, inteins in Eukaryotes are predominantly found in fungi and unicellular alga .
A homing endonuclease is often present within cis-acting inteins. The splicing and endonuclease domains are structurally and functionally distinct, as shown both by the crystal structure of Sce VMA1  and the conservation of splicing following the deletion of the homing endonuclease [1, 7]. In addition minimal or mini-inteins lacking the homing endonuclease occur naturally or have been engineered to be as small as 143 amino acids [8, 9].
The use of inteins in molecular biology, biotechnology and drug discovery has recently been reviewed [10–12]. The broad spectrum of applications for inteins is in part due to the minimal constraints placed on the sequence of the exteins. The only absolute requirement is the presence of cysteine, serine or threonine at the downstream splice junction in the C-terminal extein. There are no known size constraints. Splicing efficiency is reduced if the extein domains are unfolded or have poor solubility, but in vitro even this can usually be overcome with detergents or refolding regimes . The immediate flanking sequence of the intein can affect splicing efficiency to varying degrees , but a variety of sequences are tolerated. Thus the inherent tolerance of inteins allows them to be adapted to splice proteins of diverse character.
In this study the flexibility of inteins has been exploited to investigate the potential for inteins to direct in vivo gene modification, specifically the tagging of an internal region of a protein with GFP. The microscopy group of the Yeast Resource Center is focused on using Förster resonance energy transfer, FRET, to study the organization of large protein complexes in vivo [15–18]. A limiting step for FRET studies is identifying regions within proteins that can accept a GFP-related fluorescent protein tag. As a first step towards overcoming this limitation, we engineered genetic markers within the Pch PRP8 intein that provided a way to internally tag proteins in one step in yeast. The Pch PRP8 intein was chosen for its robust, temperature-independent splicing activity [9, 19] and the engineered inteins continued to display very high splicing efficiency.
PchPRP8 intein splices with inserts at the empty endonuclease site
During the course of their initial characterization of the Pch PRP8 mini-intein Elleuche et al.  aligned the sequence of the intein with PRP8 inteins from other species. The position of a presumptive lost homing endonuclease was identified from a gap in the alignment. This position, designated "E", later was shown to tolerate a deletion of six amino acids without loss of splicing activity . In addition the intein could be split in half at this position and the resulting two independent proteins, when expressed in E. coli, had active trans-splicing activity .
We used the experimental system of Elleuche et al.  to test whether the Pch PRP8 intein with inserted markers could splice. In their system GST is the N-terminal extein and a 6 × His-tag is the C-terminal extein. The embedded intein is expressed in E. coli and splicing results in the production of GST-6 × His and the released intein.
The modified inteins with the 4 different selection marker cassettes also showed highly efficient splicing (Figure 2, lanes 4-7). Based on the ratio of spliced products to larger, unspliced proteins in the Western blots, the splicing efficiencies ranged from ~88% for the Pch PRP8 HygR-intein to almost 100% for the His5-and LexA-VP16-inteins. Thus the Pch PRP8 intein tolerated the insertion of four different proteins at the vacant endonuclease site, maintaining nearly complete excision and splicing activity in this system.
The marked PchPRP8 inteins splice in yeast and confer selectable genetic traits
To determine whether the modified inteins would function in yeast we constructed a new panel of yeast CEN4/URA3 plasmids that contained the marked inteins expressed under the control of the GAL1 promoter. For these plasmids the N-terminal extein was GFP containing a nuclear localization signal (NLS) and the C-terminal extein was the FLAG peptide, DYKDDDDK. Just as in the previous E. coli plasmids, the extein/intein junction encodes five N-and four C-terminal extein residues of the P. chrysogenum Prp8 protein. The spliced product is NLS-GFP-FLAG (33 kD) and the released intein carries the selectable genetic marker or a transcription factor. NLS-GFP provided a simple way to follow expression and proper folding of the extein.
Concomitant with splicing the intein is released from the precursor protein. The inteins did not inhibit growth and provided resistance to G418 or hygromycin in cells expressing the Pch PRP8 G418R-intein or the HygBR-intein, respectively (Figure 3B). Likewise the Pch PRP8 His5-intein was able to complement the mutation in HIS3 in this genetic background (Figure 3B). Finally the Pch PRP8 LexA-VP16-intein was able to drive expression of a LEU2 gene that had 2 lexA operator sequences within its promoter and confer selectable leucine prototrophy to the yeast strain. Thus in all cases each marker remained functional within the context of the excised intein.
Direct integration of the marked inteins at the CMD1locus
Transformation with DNA from the Pch PRP8 G418R-, His5-and HygBR-inteins produced transformants with the selected auxotrophic or antibiotic resistance, while the Pch PRP8 LexA-VP16-intein did not yield transformants. Since the level of expression of the intein is dependent on the level of CMD1 expression we conclude that the LexA-VP16 was not at a level to fully activate lexAop 6 -LEU2 gene expression in strain YL [6, 4]LU. However in agreement with the previous results from yeast episomal expression, the recovery of resistant or prototrophic cells from the other Pch PRP8 marked-inteins again showed that the intein domain did not impair the activity of the selection markers.
Associated with the impaired growth was evidence of inefficient splicing (Figure 5B). The Pch PRP8 His5-intein was effectively spliced from calmodulin, producing Cmd1-GFP at high efficiency (lane 3). On the other hand, the G418R-intein (lanes 4,5) and HygBR-intein (not shown) were not. Different isolates showed varying levels of accumulation of unspliced product (lanes 4 and 5). The slow growth and phenotypic variation of the G418R and HygR isolates precluded further analysis and in any case meant that these inteins were not suitable for tagging Cmd1.
Calmodulin concentrates at sites of cell growth and at the spindle pole body in yeast [27–29]. The wild-type localization pattern was seen for Cmd1 with the internal GFP-His5-intein (Figure 5C). In agreement with the published results, unbudded cells had calmodulin in puncta at the cell membrane. In newly budded cells calmodulin formed a patch at the bud tip that dispersed as the bud grew. Preceding cytokinesis calmodulin was concentrated at the bud neck. Localization at the pair of spindle pole bodies is best seen in the medium budded cell in Figure 5. For comparison the online Yeast Resource Center Public Image Repository  contains over 2000 images of yeast showing the localization of CMD1 tagged with different fluorescent proteins.
Inteins are widely used in biotechnology to manipulate proteins. The capacity of inteins to break and form peptide bonds has been exploited to develop a range of methodologies from a routine procedure to purify proteins, to more advanced semi-synthetic applications that assemble proteins for NMR, crystallography and mechanistic studies. These uses and others have been recently reviewed [10, 11, 30].
The use of inteins as genetic tools is still an emerging area of research. Temperature-sensitive inteins [31–33], light-activated inteins , and inteins controlled by small molecules [35–37], provide a way to control protein activity by controlling when a protein that has been interrupted by an intein is spliced together to yield its mature form. Conditional protein assembly by inteins in vivo is a promising method to generate temperature-sensitive mutants, and target when and where a protein is functional in a cell.
Here we add a new role for inteins in genetic manipulations, as a genetic marker with the unique property of self-excision. The engineered intein marks the expression of the protein into which it is embedded and yet after splicing is not a part of the mature protein. We demonstrate in yeast the use of a Pch PRP8 His5-marked intein to deliver, in one step and in vivo, GFP within a targeted protein without disturbing the function of the protein. In principle, the method could be extended to other protein tags, and adapted to other organisms.
We created a family of genetically marked inteins based on the PRP8 intein of Penicillium chrysogenum. PRP8 inteins have an ancient heritage and are widespread [19, 38–40]. However they are not found in S. cerevisiae. The Pch PRP8 intein and the native yeast VMA1 intein are sufficiently divergent (21% sequence identity) to preclude homologous recombination between the two during the transformation step. For comparison the S. cerevisiae HIS3 and the S. pombe his5 + genes share 58% sequence identity and yet do not recombine during integrative transformation . The sequence divergence, the fact that the Pch PRP8 intein is well characterized [9, 19, 20, 39], splices at high efficiency across a broad range of temperatures  and lacks a DNA binding domain led to its choice in our studies.
The primary sequence of the Pch PRP8 mini-intein shares sequence similarity with other inteins . Thus its three-dimensional structure likely conforms to the canonical Hedgehog/Intein (HINT) protein scaffold [42–48]. The HINT module has a flat horseshoe shape with a series of β-strands forming the framework. The active-site is centered at the interior base or "toe" of the horseshoe. Endonucleases if present in the intein are a separate domain located away from the active site region at the "heel" of the horseshoe.
Here we show that the Pch PRP 8 intein, and presumably other inteins, have a remarkable capacity to accept foreign protein domains at the region of the structure where the homing endonuclease resides in full inteins. High splicing efficiency was observed for all of the inserted single domain proteins that were used for genetic markers. We attribute the success to several factors. An alignment of the Pch PRP8 intein with the Synechocystis sp. DnaB and the Mycobacterium xenopi GyrA inteins shows that the insertion site for our genetic markers is located in disordered loops in the crystal structures of both of these inteins [42, 44]. Thus the region of insertion is inherently flexible and unstructured. In addition, one shared feature of the proteins used for markers is that their N-and C-termini are proximal [22–25]. The exit of the inserted protein domain out of the intein and its return occurs nearby in space and would tend to minimize strain in the structure of the intein. Since the majority of single domain proteins have some region of their two termini within 5 Å [49, 50] and termini are consistently found at the surface of the protein , we predict that many single domain proteins could be inserted into inteins without detriment to splicing efficiency.
Three enzymes and a transcription factor were used as genetic markers to modify the Pch PRP8 intein. In pilot experiments all four modified inteins showed high splicing efficiency, both in E. coli and yeast, and in yeast the proteins within the excised inteins were active and enabled growth under conditions of selection. However when these inteins were used to deliver GFP within calmodulin only the strain tagged with the Pch PRP8 His5-intein grew normally. Under selective conditions transformation with the Pch PRP8 LexA-VP16-intein did not yield any transformants, and the G418R-and HygR-inteins yielded cells with poor growth and diminished splicing efficiency. The disruption of calmodulin function was surprising, given the pilot results, and is not understood. It is possible that the unspliced products which accumulate interfere with growth. We are actively investigating ways to boost the level of stable protein and the efficiency of splicing through changes in codon usage and the composition of the linkers between the various domains. In addition, given the success of the Pch PRP8 His5-intein, we are constructing other inteins with metabolic markers that may require lower levels of expression.
Our intein based method to insert the gene encoding GFP within an ORF offers several advantages over other PCR-based methods available in yeast. Other methods [52–54] introduce a gene with its own promoter that must be first be removed before the GFP is correctly in frame. Thus a second recombination event is required, either catalyzed by the induction of Cre-recombinase or by a rare natural recombination event that is isolated by genetic selection, to remove the genetic marker with its associated promoter. In addition the Cre-recombinase based system leaves behind within the ORF a peptide sequence coded for by the remaining loxP site, a sequence that can disrupt activity (unpublished). The introduced gene also temporarily interrupts the expression of the target protein. With the intein-based method integration and tagging occur in one step. Since the marked inteins are inserted in frame, expression of the target is only disrupted during recombination. Given these advantages marked inteins show great promise and complement other available methods.
We have shown that the Pch PRP8 intein can incorporate a variety of selectable genetic markers and still exhibit high splicing activity. This led to the development of a simple method to insert GFP within the interior of a protein in yeast. This novel use of an intein has the potential to provide a way to add, substitute, or delete domains within targeted proteins in a single step in yeast. In addition it may find application in other systems as well, such as recombineering, a method that is also based on genetic manipulation through recombination.
Refinement of this method is underway at the Yeast Resource Center. Primary areas to investigate will be the effect of codon optimization, the utility of other selection markers, and cassette development for other useful tags such as the FRET partners YFP and CFP.
Strains and media
KGY315  (MATa/MATα, ade2-1oc/ade2-1oc, ADE3/ade3Δ100, can1-100/can1-100, CYH2 s /cyh2 r , his3-11,15/his3-11,15, leu2-3,112/leu2-3,112, trp1-1/trp1-1, ura3-1/ura3-1) was derived from W303 . YL(2, 4)LU (MATa, his3, trp1, leu2::lexAop 2 -LEU2, ura3::lexAop 4 -SPO13TATA-URA3) is a reporter strain for LexA-VP16 expression . YL(6, 4)LU is the same as YL(2, 4)LU except that there are 6 lexAop sequences before LEU2. LAY18-2C was derived from KGY315 in which the C-terminus of CMD1 was tagged with GFP as described at the Yeast Resource Center web site http://depts.washington.edu/yeastrc/pages/fm_3.html. Escherichia coli strain Tuner™(DE3) (EMB, San Diego, CA) was used for expression and XL10-Gold® (Stratagene, La Jolla, CA) for routine cloning.
Yeast were grown in either synthetic dextrose minimal media (SD) or synthetic galactose minimal media with appropriate supplements, or yeast peptone dextrose-rich medium (YPD) with added antibiotics . E. coli was grown in NZCYM medium with antibiotics.
Plasmid pGPch-1 (gift of Stefanie Pöggeler)  was the source of the Pch PRP8 intein and contains an N-terminal extein of glutathione S-transferase (GST) and a C-terminal extein of a 6 × His epitope tag. pRR01 is derived from pGPch-1. The ΔE sequence , 5'-GGCGCCGATGATTCGGCT-3', coding for GADDSA, was replaced with 5'-GGCGCCGCAG GTGCAGGTGC AGGTGCAGCT AGCGCAGCAG GTACCGCGGG TGCTGGTGCT GGTGCTTCGG CT-3' (NheI and KpnI sites underlined), to code for the protein sequence GAAGAGAGAASAAGTAGAGAGASA. Six codons within the intein sequence were optimized for expression in yeast by site-directed mutagenesis using the QuikChange® protocol (Stratagene, La Jolla, CA): Leu2 (CTC to TTG); Gly5 (GGG to GGA); Leu8 (CTC to TTG); Arg10 (CGA to AGA); Arg46 (CGC to AGA); and Arg49 (CGC to AGA). Numbering is relative to Cys (in the sequence CLAK) at position 1.
Plasmids pRR02-05 were derived from pRR1. The G418 R gene, the Hyg R gene, the his5 + gene of Schizosaccharomyces pombe and the lexA-VP16 fusion were PCR amplified from pBS7, pBS4, and pDH5 (YRC, University of Washington)  and pBS LexA::VP16-SV40 (gift of Tzumin Lee, Janelia Farm Research Campus) , respectively. The ORFs were amplified with NheI and KpnI ends and inserted at the NheI/KpnI junction in pRR01. All amplifications for cloning were carried out using VentR® DNA polymerase (New England Biolabs, Beverly, MA).
Plasmids pRR11-15 and pRR21-25 include the intein sequence from the corresponding marked inteins in the pRR02-05 series. However the N-extein is either GFP or GFP with a nuclear localization signal, NLS-GFP, and the C-extein is a FLAG® epitope. In addition protein linker sequences have been inserted at the junctions between ORFs. These linkers, L2-L5 in Figure 4, encode the following protein sequences, with adjacent sequences in parentheses: L2, (...LYK)-GAGAGAGAGAG-(FWEKACLAK...); L3, (...KGA)-AGAGAGAA-(SG...); L4, AGAGAGA-(SAQ....); and L5, (SGFE)-GSG. The underlined sequences are from the N-and C-terminal exteins of P. chrysogenum Prp8.
GST[Pch PRP8 linker]HIS6
GST[Pch PRP8 G418R]HIS6
GST[Pch PRP8 HIS5]HIS6
GST[Pch PRP8 HygBR]HIS6
GST[Pch PRP8 LexA-VP16]HIS6
GFP[Pch PRP8 linker]FLAG
GFP[Pch PRP8 G418R]FLAG
GFP[Pch PRP8 HIS5]FLAG
GFP[Pch PRP8 HygBR]FLAG
GFP[Pch PRP8 LexA-VP16]FLAG
NLS-GFP[Pch PRP8 linker]FLAG
NLS-GFP[Pch PRP8 G418R]FLAG
NLS-GFP[Pch PRP8 HIS5]FLAG
NLS-GFP[Pch PRP8 HygBR]FLAG
NLS-GFP[Pch PRP8 LexA-VP16]FLAG
NLS-GFP (no intein)
Episomal expression of inteins in E. coli and yeast
Expression in E. coli was performed in Tuner™ (DE3) with pLysSRARE to express rare tRNAs. Bacteria were grown to early log phase in NZCYM medium supplemented with ampicillin (100 μg/ml) and chloramphenicol (34 μg/ml) then induced with 0.2 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 17 h at 20°. Culture densities were measured with a Klett-Summerson Colorimeter.
For plasmid-based expression in yeast from the GAL1 promoter, the strain YL (2,4) LU was used with various plasmids based on pBM258. Cells were grown overnight at 30°in S-medium -ura + 2% raffinose. When cells reached a turbidity of 20 Klett units, galactose was added to a final concentration of 2% and growth was continued to 100 Klett units.
Western blot analysis and the estimation of splicing efficiency
Equivalent amounts of cells were pelleted, precipitated with 10% TCA , resuspended in sample buffer and separated by SDS-PAGE. Three identical gels were prepared: one directly stained by Coomassie® Brilliant Blue for detection of total protein and two transferred to Immobilon™ (Millipore, Billerica, MA) for Western blot analysis. Antibodies were either directed against GST (Santa Cruz Biotechnology, Santa Cruz, CA), Penta-His (Qiagen, Valencia, CA), GFP (Roche Applied Science, Indianapolis, IN) or the FLAG epitope (anti-FLAG® M2, Sigma-Alrich, St. Louis, MO). Antibodies were detected using Alexa Fluor 680 anti-IgG secondary (Invitrogen, Carlsbad, CA) and visualized on an Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE). The Odyssey software was used to quantify band intensity. To estimate the limits of detection for the anti-GFP antibody, Western blot analysis was performed on serial dilutions of extracts from strain YL (2,4) LU expressing NLS-GFP from plasmid pRR26.
Genomic integration of marked inteins into the CMD1locus
The intein cassettes were amplified from the pRR11-15 plasmids using the Expand Long Template PCR System (Roche, Indianapolis, IN). Primers included a 40 base extension homologous to the target CMD1 gene, a short linker sequence encoding L1 and L5 in Figure 4, and ~ 23 bp homologous to the ends of the intein sequence. The sequences of the primers were: 5'-TAGTGAATTT TTGGCTCTGA TGTCTCGTCA ACTCAAATCA GGTTCAGGT AGTAAAGGAG AAGAACTTTT CACTG-3' and 5'-ATACTTTAAA AGCTTCTAGT AGTTCTTGTT CAGAGTCATT TCCAGATCC TTCGAAACCA CTGTTGTGCA G-3' in which bold sequence is homologous to the intein cassette and underlined is homologous to the CMD1 site of insertion. The amplification and integration protocols are described at the Yeast Resource Center web site http://depts.washington.edu/yeastrc/pages/fm_3.html. KGY315 was the host except in the case of the transformation of the LexA-VP16-intein, which was transformed into YL [6, 4] LU.
Fluorescence microscopy was performed on a DeltaVision® imaging system (Applied Precision, Issaquah, WA) as previously reported . Protein extraction and Western blot analysis were performed as described above using anti-GFP and anti-calmodulin antibodies . The correct integration of the inteins was confirmed by four different PCR reactions using the genomic DNA as template and primer pairs from these regions: 5' UTR of CMD1 as forward primer (f.p.) × GFP as reverse primer (r.p.); GFP (f.p.) × C-terminal intein (r.p.); C-terminal intein (f.p.) × 3' UTR of CMD1(r.p.); and 5' UTR of CMD1 (f.p.) × 3' UTR of CMD1(r.p.).
We would like to thank Stefanie Pöggeler for the gift of pGPch-1 and Tzumin Lee for the gift of pBS LexA-VP16 SV40. We would like to thank Simon Diep and Jing Huang for strain YL(6,4)LU and plasmids. This work is supported by the National Center for Research Resources of the National Institutes of Health by a grant to T.N.D. entitled 'Comprehensive Biology: Exploiting the Yeast Genome', P41 RR011823.
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