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Figure 1 | BMC Biotechnology

Figure 1

From: An intein with genetically selectable markers provides a new approach to internally label proteins with GFP

Figure 1

A schematic of genetic selection using marked Pch PRP8 inteins. N-and C-terminal exteins, boxed in blue, flank the intein, boxed in red, as shown. Following translation the intein catalyzes the ligation of the exteins and the Pch PRP8 marker-intein is released. Markers used for genetic selection were inserted between amino acid positions 114 and 115 of the intein.

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