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  1. Natural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current of...

    Authors: Laurence O. W. Wilson, Sara Hetzel, Christopher Pockrandt, Knut Reinert and Denis C. Bauer
    Citation: BMC Biotechnology 2019 19:40
  2. Aerobic side chain degradation of phytosterols by actinobacteria is the basis for the industrial production of androstane steroids which are the starting materials for the synthesis of steroid hormones. A nati...

    Authors: Eugeny Y. Bragin, Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Dmitry V. Dovbnya and Marina V. Donova
    Citation: BMC Biotechnology 2019 19:39
  3. Anti-Tumor Necrosis Factor-alpha therapy has become clinically important for treating inflammatory bowel disease. However, the use of conventional immunotherapy requires a systemic exposure of patients and col...

    Authors: Maria José Chiabai, Juliana Franco Almeida, Mariana Gabriela Dantas de Azevedo, Suelen Soares Fernandes, Vanessa Bastos Pereira, Raffael Júnio Araújo de Castro, Márcio Sousa Jerônimo, Isabel Garcia Sousa, Leonora Maciel de Souza Vianna, Anderson Miyoshi, Anamelia Lorenzetti Bocca, Andrea Queiroz Maranhão and Marcelo Macedo Brigido
    Citation: BMC Biotechnology 2019 19:38
  4. CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly cont...

    Authors: Bent Larsen Petersen, Svenning Rune Möller, Jozef Mravec, Bodil Jørgensen, Mikkel Christensen, Ying Liu, Hans H. Wandall, Eric Paul Bennett and Zhang Yang
    Citation: BMC Biotechnology 2019 19:36
  5. Viral vectors are invaluable tools to transfer genes and/or regulatory sequences into differentiated cells such as pancreatic cells. To date, several kinds of viral vectors have been used to transduce differen...

    Authors: Olivier Albagli, Alicia Maugein, Lukas Huijbregts, Delphine Bredel, Géraldine Carlier, Patrick Martin and Raphaël Scharfmann
    Citation: BMC Biotechnology 2019 19:35
  6. In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more tha...

    Authors: Sin-Yeang Teow, Kitson Liew, Mohd Firdaus Che Mat, Marini Marzuki, Norazlin Abdul Aziz, Tai-Lin Chu, Munirah Ahmad and Alan Soo-Beng Khoo
    Citation: BMC Biotechnology 2019 19:34
  7. Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify mo...

    Authors: Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark and Margaret C. M. Smith
    Citation: BMC Biotechnology 2019 19:32
  8. Copy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders. Here we investigate a CNV on chromosome 10q11.22 that spans NPY4R, the gene ...

    Authors: Kateryna Shebanits, Torsten Günther, Anna C. V. Johansson, Khurram Maqbool, Lars Feuk, Mattias Jakobsson and Dan Larhammar
    Citation: BMC Biotechnology 2019 19:31
  9. The potassium dichromate oxidation method used in determination of alcohols in fermentation has two major disadvantages. This method cannot be used to determine alcohols in raw fermentation broth samples, whic...

    Authors: Peng Zhang, Hao Hai, Dongxu Sun, Weihua Yuan, Weijie Liu, Ruru Ding, Mengting Teng, Lin Ma, Jun Tian and Caifa Chen
    Citation: BMC Biotechnology 2019 19:30
  10. Several types of phospholipases have been described in phospholipids modification. The majority of phospholipase D (PLD) superfamily members can catalyze two separate reactions: the hydrolysis of phospholipids...

    Authors: Haiyang Zhang, Wenqin Chu, Jianan Sun, Zhen Liu, Wen-can Huang, Changhu Xue and Xiangzhao Mao
    Citation: BMC Biotechnology 2019 19:29
  11. In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumum...

    Authors: Tao Yu, Chunxia Qiao, Ming Lv and Luqun Tang
    Citation: BMC Biotechnology 2019 19:28
  12. The current limitations of conventional BCG vaccines highlights the importance in developing novel and effective vaccines against tuberculosis (TB). The utilization of probiotics such as Lactobacillus plantarum f...

    Authors: Anhar Danial Mustafa, Jeevanathan Kalyanasundram, Sarah Sabidi, Adelene Ai-Lian Song, Maha Abdullah, Raha Abdul Rahim and Khatijah Yusoff
    Citation: BMC Biotechnology 2019 19:27
  13. Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expr...

    Authors: M. Alejandro Carballo-Amador, Edward A. McKenzie, Alan J. Dickson and Jim Warwicker
    Citation: BMC Biotechnology 2019 19:26
  14. The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the effic...

    Authors: Kayla Carey, Junghyun Ryu, Kyungjun Uh, Andrea J. Lengi, Sherrie Clark-Deener, Benjamin A. Corl and Kiho Lee
    Citation: BMC Biotechnology 2019 19:25
  15. Increasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B

    Authors: Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen and Guohao He
    Citation: BMC Biotechnology 2019 19:24
  16. The gene transduction efficiency of adenovirus to hematopoietic cells, especially T lymphocytes, is needed to be improved. The purpose of this study is to improve the transduction efficiency of T lymphocytes b...

    Authors: Yun Lv, Feng-Jun Xiao, Yi Wang, Xiao-Hui Zou, Hua Wang, Hai-Yan Wang, Li-Sheng Wang and Zhuo-Zhuang Lu
    Citation: BMC Biotechnology 2019 19:23
  17. The importance of the accessory enzymes such as α-L-arabinofuranosidases (AFases) in synergistic interactions within cellulolytic mixtures has introduced a paradigm shift in the search for hydrolytic enzymes. ...

    Authors: Brent Fortune, Sizwe Mhlongo, Leonardo Joaquim van Zyl, Robert Huddy, Mariette Smart and Marla Trindade
    Citation: BMC Biotechnology 2019 19:22
  18. A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in...

    Authors: Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, Dawn M. Kilkenny and Julie Audet
    Citation: BMC Biotechnology 2019 19:21
  19. Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable f...

    Authors: Régis P. Lemaitre, Aliona Bogdanova, Barbara Borgonovo, Jeffrey B. Woodruff and David N. Drechsel
    Citation: BMC Biotechnology 2019 19:20
  20. Recently, it has been indicated that anaerobic co-digestion of waste activated sludge with other waste streams at wastewater treatment plants is a promising strategy for enhancing methane production and materi...

    Authors: Seyed Mostafa Hallaji, Mohammad Kuroshkarim and Seyede Parvin Moussavi
    Citation: BMC Biotechnology 2019 19:19
  21. The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editi...

    Authors: Soumaya Najah, Corinne Saulnier, Jean-Luc Pernodet and Stéphanie Bury-Moné
    Citation: BMC Biotechnology 2019 19:18
  22. Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The organism has been proposed to be a suitable organism...

    Authors: Alexandria T. N. Holland, Michael J. Danson and Albert Bolhuis
    Citation: BMC Biotechnology 2019 19:17
  23. Norcantharidin (NCTD), a demethylated derivative of cantharidin (defensive toxin of blister beetles), has been reported to exhibit insecticidal activity against various types of agricultural pests. However, NC...

    Authors: Liya Zeng, Yongchang Liu, Jun Pan and Xiaowen Liu
    Citation: BMC Biotechnology 2019 19:16
  24. Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To exte...

    Authors: Guilherme Souza Prado, Pingdwende Kader Aziz Bamogo, Joel Antônio Cordeiro de Abreu, François-Xavier Gillet, Vanessa Olinto dos Santos, Maria Cristina Mattar Silva, Jean-Paul Brizard, Marcelo Porto Bemquerer, Martine Bangratz, Christophe Brugidou, Drissa Sérémé, Maria Fatima Grossi-de-Sa and Séverine Lacombe
    Citation: BMC Biotechnology 2019 19:15
  25. Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical...

    Authors: Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim and Kwang-Chul Kwon
    Citation: BMC Biotechnology 2019 19:13
  26. Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below:

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III
    Citation: BMC Biotechnology 2019 19:12

    The original article was published in BMC Biotechnology 2019 19:5

  27. Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study ...

    Authors: Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar and Waldemar Rymowicz
    Citation: BMC Biotechnology 2019 19:11
  28. Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC...

    Authors: Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang and Weibing Xu
    Citation: BMC Biotechnology 2019 19:10
  29. Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system...

    Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang
    Citation: BMC Biotechnology 2019 19:9

    The Correction to this article has been published in BMC Biotechnology 2020 20:42

  30. We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter...

    Authors: Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani and Vadood Razavilar
    Citation: BMC Biotechnology 2019 19:8
  31. Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and effici...

    Authors: Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen, Weizhi Ji and Yuyu Niu
    Citation: BMC Biotechnology 2019 19:7
  32. MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

    Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu
    Citation: BMC Biotechnology 2019 19:6
  33. Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic or...

    Authors: Matthew P. A. Bellefleur, Soo-Young Wanda and Roy Curtiss III
    Citation: BMC Biotechnology 2019 19:5

    The Correction to this article has been published in BMC Biotechnology 2019 19:12

  34. Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent yea...

    Authors: Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid and Supachai Vuttipongchaikij
    Citation: BMC Biotechnology 2019 19:4
  35. A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterol...

    Authors: Nguyen Thanh Trung, Nguyen Minh Hung, Nguyen Huy Thuan, Nguyen Xuan Canh, Thomas Schweder and Britta Jürgen
    Citation: BMC Biotechnology 2019 19:3
  36. Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Rece...

    Authors: Kiyoung Eun, Min Gi Park, Yeon Woo Jeong, Yeon Ik Jeong, Sang-Hwan Hyun, Woo Suk Hwang, Sung-Hak Kim and Hyunggee Kim
    Citation: BMC Biotechnology 2019 19:1
  37. Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the prote...

    Authors: Pollyana Santos Queiroz, France Anne Dias Ruas, Natália Rocha Barboza, William de Castro Borges and Renata Guerra-Sá
    Citation: BMC Biotechnology 2018 18:83
  38. The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manuf...

    Authors: Lissa R. Herron, Clare Pridans, Matthew L. Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel J. Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David A. Hume, Andrew C. Gill and Helen M. Sang
    Citation: BMC Biotechnology 2018 18:82
  39. The methanol-regulated AOX1 promoter (PAOX1) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol d...

    Authors: Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong and Ching-Tsan Huang
    Citation: BMC Biotechnology 2018 18:81
  40. More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between differe...

    Authors: Zheng-Wei Qu, Si-Ya Zhou, Shi-Xin Guan, Rui Gao, Zuo-Wen Duan, Xin Zhang, Wei-Yan Sun, Wen-Li Fan, Shui-Sen Chen, Li-Jing Chen, Jing-Wei Lin and Yan-Ye Ruan
    Citation: BMC Biotechnology 2018 18:80
  41. Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complica...

    Authors: Ronald R. Navarro, Yuichiro Otsuka, Masanobu Nojiri, Shigehiro Ishizuka, Masaya Nakamura, Kazuhiro Shikinaka, Kenji Matsuo, Kei Sasaki, Ken Sasaki, Kazuhide Kimbara, Yutaka Nakashimada and Junichi Kato
    Citation: BMC Biotechnology 2018 18:79
  42. Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfol...

    Authors: Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro, Luís Carlos de Souza Ferreira and Ligia Morganti
    Citation: BMC Biotechnology 2018 18:78
  43. Rift Valley fever virus (RVFV), the causative agent of Rift Valley fever, is an enveloped single-stranded negative-sense RNA virus in the genus Phlebovirus, family Bunyaviridae. The virus is spread by infected mo...

    Authors: Sandiswa Mbewana, Ann E. Meyers, Brandon Weber, Vuyokazi Mareledwane, Maryke L. Ferreira, Phelix A. O. Majiwa and Edward P. Rybicki
    Citation: BMC Biotechnology 2018 18:77
  44. Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated b...

    Authors: Jonathan Bouvette, Dursun Nizam Korkut, Aurélien Fouillen, Soumiya Amellah, Antonio Nanci, Yves Durocher, James G. Omichinski and Pascale Legault
    Citation: BMC Biotechnology 2018 18:76
  45. The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiat...

    Authors: Aijia Cai, Moritz Hardt, Paul Schneider, Rafael Schmid, Claudia Lange, Dirk Dippold, Dirk W. Schubert, Anja M. Boos, Annika Weigand, Andreas Arkudas, Raymund E. Horch and Justus P. Beier
    Citation: BMC Biotechnology 2018 18:75
  46. This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed...

    Authors: Abebe Gebeyehu, Nurelegne Shebeshe, Helmut Kloos and Solomon Belay
    Citation: BMC Biotechnology 2018 18:74

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