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  1. Content type: Research article

    Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing ind...

    Authors: Hong Zhu, L. Bruce Reynolds and Rima Menassa

    Citation: BMC Biotechnology 2017 17:53

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  2. Content type: Research article

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Authors: Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    Citation: BMC Biotechnology 2017 17:52

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  3. Content type: Research article

    The discharge of poorly treated effluents into the environment has far reaching, consequential impacts on human and aquatic life forms. Thus, we evaluated the flocculating efficiency of our test bioflocculant ...

    Authors: Mayowa Oladele Agunbiade, Esta Van Heerden, Carolina H. Pohl and Anofi Tom Ashafa

    Citation: BMC Biotechnology 2017 17:51

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  4. Content type: Research article

    Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only...

    Authors: Yan-Jun Li, Shou-Hong Zhu, Xin-Yu Zhang, Yong-Chang Liu, Fei Xue, Lan-Jie Zhao and Jie Sun

    Citation: BMC Biotechnology 2017 17:50

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  5. Content type: Research article

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Authors: Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    Citation: BMC Biotechnology 2017 17:49

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  6. Content type: Research article

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by...

    Authors: Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: BMC Biotechnology 2017 17:48

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  10. Content type: Research article

    Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is pre...

    Authors: Martinus J.A. Daas, Patricia Murciano Martínez, Antonius H.P. van de Weijer, John van der Oost, Willem M. de Vos, Mirjam A. Kabel and Richard van Kranenburg

    Citation: BMC Biotechnology 2017 17:44

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  11. Content type: Methodology article

    Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these metho...

    Authors: Lisa A. Santry, Joelle C. Ingrao, Darrick L. Yu, Jondavid G. de Jong, Laura P. van Lieshout, Geoffrey A. Wood and Sarah K. Wootton

    Citation: BMC Biotechnology 2017 17:43

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  12. Content type: Research article

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Authors: Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    Citation: BMC Biotechnology 2017 17:42

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  13. Content type: Research Article

    At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated me...

    Authors: Darjan Andrejc, Alenka Možir and Matic Legiša

    Citation: BMC Biotechnology 2017 17:41

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  14. Content type: Research article

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Authors: Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    Citation: BMC Biotechnology 2017 17:40

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  16. Content type: Research article

    Propionate is widely used as an important preservative and important chemical intermediate for synthesis of cellulose fibers, herbicides, perfumes and pharmaceuticals. Biosynthetic propionate has mainly been p...

    Authors: Jing Li, Xinna Zhu, Jing Chen, Dongdong Zhao, Xueli Zhang and Changhao Bi

    Citation: BMC Biotechnology 2017 17:38

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  17. Content type: Research article

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

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  18. Content type: Research article

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Authors: Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    Citation: BMC Biotechnology 2017 17:36

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  21. Content type: Research article

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Authors: Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    Citation: BMC Biotechnology 2017 17:33

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  22. Content type: Methodology article

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Authors: Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong and Yibin Lin

    Citation: BMC Biotechnology 2017 17:32

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  23. Content type: Research article

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Authors: Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    Citation: BMC Biotechnology 2017 17:31

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  25. Content type: Methodology article

    Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Authors: Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    Citation: BMC Biotechnology 2017 17:29

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  26. Content type: Research article

    The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Authors: Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    Citation: BMC Biotechnology 2017 17:28

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  27. Content type: Research article

    Oleaginous organisms are a promising, renewable source of single cell oil. Lipid accumulation is mainly induced by limitation of nutrients such as nitrogen, phosphorus or sulfur. The oleaginous yeast Trichosporon...

    Authors: Felix Bracharz, Veronika Redai, Kathrin Bach, Farah Qoura and Thomas Brück

    Citation: BMC Biotechnology 2017 17:27

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  28. Content type: Research article

    Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown.

    Authors: Yihua Zhan, Xiangyu Sun, Guozeng Rong, Chunxiao Hou, Yingying Huang, Dean Jiang and Xiaoyan Weng

    Citation: BMC Biotechnology 2017 17:26

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  29. Content type: Research article

    Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile...

    Authors: Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen and Yuanlin Xi

    Citation: BMC Biotechnology 2017 17:25

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  30. Content type: Methodology article

    Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian...

    Authors: Sander B. Frank, Veronique V. Schulz and Cindy K. Miranti

    Citation: BMC Biotechnology 2017 17:24

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  31. Content type: Research article

    Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Authors: Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    Citation: BMC Biotechnology 2017 17:23

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  32. Content type: Research article

    α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Authors: Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2017 17:22

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  33. Content type: Research article

    Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Authors: Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    Citation: BMC Biotechnology 2017 17:21

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  34. Content type: Research article

    poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-sca...

    Authors: Jun Xia, Jiaxing Xu, Xiaoyan Liu, Jiming Xu, Xingfeng Wang and Xiangqian Li

    Citation: BMC Biotechnology 2017 17:20

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  35. Content type: Research article

    Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basi...

    Authors: Hui Xu, Meng-Yuan Guo, Yan-Hua Gao, Xiao-Hui Bai and Xuan-Wei Zhou

    Citation: BMC Biotechnology 2017 17:19

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  36. Content type: Research article

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Authors: Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    Citation: BMC Biotechnology 2017 17:18

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  37. Content type: Research article

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Authors: Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    Citation: BMC Biotechnology 2017 17:17

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  38. Content type: Research article

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Authors: Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    Citation: BMC Biotechnology 2017 17:16

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  39. Content type: Research article

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Authors: Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    Citation: BMC Biotechnology 2017 17:15

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  40. Content type: Research article

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Authors: Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    Citation: BMC Biotechnology 2017 17:14

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  41. Content type: Methodology article

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Authors: Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    Citation: BMC Biotechnology 2017 17:13

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  42. Content type: Research article

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Authors: Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    Citation: BMC Biotechnology 2017 17:12

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  43. Content type: Research article

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Authors: Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    Citation: BMC Biotechnology 2017 17:11

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  45. Content type: Research article

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Authors: Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    Citation: BMC Biotechnology 2017 17:9

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  46. Content type: Research article

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Authors: Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    Citation: BMC Biotechnology 2017 17:8

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  47. Content type: Research article

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Authors: Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    Citation: BMC Biotechnology 2017 17:7

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  48. Content type: Research article

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Authors: Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    Citation: BMC Biotechnology 2017 17:6

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  50. Content type: Research article

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    Authors: D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    Citation: BMC Biotechnology 2017 17:4

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