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  1. Content type: Research article

    When the unavoidable waste generation is considered as damaging to our environment, it becomes crucial to develop a sustainable technology to remediate the pollutant source towards an environmental protection ...

    Authors: Arezoo Dadrasnia, Mohd Sofian Azirun and Salmah Binti Ismail

    Citation: BMC Biotechnology 2017 17:85

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  2. Content type: Research article

    The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluat...

    Authors: Zhen Chen, Zhipeng Li, Peize Liu, Yu Liu, Yuanpeng Wang, Qingbiao Li and Ning He

    Citation: BMC Biotechnology 2017 17:84

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  4. Content type: Research article

    Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4]−, [Cl]−, [MeCO2]−) were...

    Authors: Zhao Wang, John Gräsvik, Leif J. Jönsson and Sandra Winestrand

    Citation: BMC Biotechnology 2017 17:82

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  5. Content type: Methodology article

    Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembl...

    Authors: Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong and Yibin Lin

    Citation: BMC Biotechnology 2017 17:81

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  6. Content type: Methodology article

    There is a need for the development of synthetic biology methods and tools to facilitate rapid and efficient engineering of yeast that accommodates the needs of specific biotechnology projects. In particular, ...

    Authors: Ana Pérez-González, Ryan Kniewel, Marcel Veldhuizen, Hemant K. Verma, Mónica Navarro-Rodríguez, Luis M. Rubio and Elena Caro

    Citation: BMC Biotechnology 2017 17:80

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  7. Content type: Research article

    The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus ...

    Authors: Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J. Stonehouse…

    Citation: BMC Biotechnology 2017 17:79

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  8. Content type: Research article

    Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). ...

    Authors: Mary Ladidi Abu, Hisham Mohd Nooh, Siti Nurbaya Oslan and Abu Bakar Salleh

    Citation: BMC Biotechnology 2017 17:78

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  9. Content type: Research article

    Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits a highly restricted tropism for cells of the monocyte-macrophage lineage, utilizing porcine CD163 (pCD163) as an indispensable cellular rece...

    Authors: Liangliang Li, Chunyan Wu, Gaopeng Hou, Biyun Xue, Sha Xie, Qin Zhao, Yuchen Nan, Gaiping Zhang and En-Min Zhou

    Citation: BMC Biotechnology 2017 17:77

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  10. Content type: Research article

    The myxomycetes derive their common name (slime molds) from the multinucleate trophic stage (plasmodium) in the life cycle, which typically produces a noticeable amount of slimy materials, some of which is nor...

    Authors: Tuyen T.M Huynh, Trung V. Phung, Steven L. Stephenson and Hanh T.M Tran

    Citation: BMC Biotechnology 2017 17:76

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  11. Content type: Research article

    Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antiba...

    Authors: Natalia Inés Almasia, María Paula Molinari, Guillermo Andrés Maroniche, Vanesa Nahirñak, María Pilar Barrios Barón, Oscar Alberto Taboga and Cecilia Vazquez Rovere

    Citation: BMC Biotechnology 2017 17:75

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  12. Content type: Research article

    β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the te...

    Authors: Fei Chang, Xianbing Zhang, Yu Pan, Youxue Lu, Wei Fang, Zemin Fang and Yazhong Xiao

    Citation: BMC Biotechnology 2017 17:74

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  13. Content type: Research article

    Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and ...

    Authors: Rong Li, Zhenzhen Yan, Juan Wang, Qisheng Song and Zhi Wang

    Citation: BMC Biotechnology 2017 17:73

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  14. Content type: Research article

    α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent n...

    Authors: Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang and Danke Xu

    Citation: BMC Biotechnology 2017 17:72

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  15. Content type: Research article

    Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards t...

    Authors: Beatriz Temer, Leandro Vieira dos Santos, Victor Augusti Negri, Juliana Pimentel Galhardo, Pedro Henrique Mello Magalhães, Juliana José, Cidnei Marschalk, Thamy Lívia Ribeiro Corrêa, Marcelo Falsarella Carazzolle and Gonçalo Amarante Guimarães Pereira

    Citation: BMC Biotechnology 2017 17:71

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  16. Content type: Research article

    Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed...

    Authors: Aldricho Alpha Pollardo, Hong-shik Lee, Dohoon Lee, Sangyong Kim and Jaehoon Kim

    Citation: BMC Biotechnology 2017 17:70

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  18. Content type: Research article

    Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen ...

    Authors: Huidan Zhang, Yingang Feng, Qiu Cui and Xiaojin Song

    Citation: BMC Biotechnology 2017 17:68

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  19. Content type: Methodology article

    Genetically modified crops (GM crops) have been developed to improve the agricultural traits of modern crop cultivars. Safety assessments of GM crops are of paramount importance in research at developmental st...

    Authors: Doori Park, Su-Hyun Park, Yong Wook Ban, Youn Shic Kim, Kyoung-Cheul Park, Nam-Soo Kim, Ju-Kon Kim and Ik-Young Choi

    Citation: BMC Biotechnology 2017 17:67

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  21. Content type: Research article

    Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engr...

    Authors: Rebecca L. L. Moore, Matthew J. Worrallo, Peter D. Mitchell, Jon Harriman, Katie E. Glen and Robert J. Thomas

    Citation: BMC Biotechnology 2017 17:65

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  22. Content type: Research article

    Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel C...

    Authors: Maniraj Rathinam, Shweta Singh, Debasis Pattanayak and Rohini Sreevathsa

    Citation: BMC Biotechnology 2017 17:64

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  23. Content type: Research article

    Acetic acid is a predominant by-product of lignocellulosic biofuel process, which inhibits microbial biocatalysts. Development of bacterial strains that are tolerant to acetic acid is challenging due to poor u...

    Authors: Yu-Fan Liu, Chia-Wen Hsieh, Yao-Sheng Chang and Being-Sun Wung

    Citation: BMC Biotechnology 2017 17:63

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  24. Content type: Methodology article

    Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 ...

    Authors: Michael J. Bland, Magaly Ducos-Galand, Marie-Eve Val and Didier Mazel

    Citation: BMC Biotechnology 2017 17:62

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  25. Content type: Methodology article

    The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

    Authors: Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

    Citation: BMC Biotechnology 2017 17:61

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  26. Content type: Research article

    Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid ...

    Authors: Karim E. Jaén, Juan-Carlos Sigala, Roberto Olivares-Hernández, Karsten Niehaus and Alvaro R. Lara

    Citation: BMC Biotechnology 2017 17:60

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  27. Content type: Research article

    The environmental gliding bacteria Lysobacter are emerging as a new group of biocontrol agents due to their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsi...

    Authors: Wei Zhang, Justin Huffman, Shengying Li, Yuemao Shen and Liangcheng Du

    Citation: BMC Biotechnology 2017 17:59

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  28. Content type: Research article

    Fibroblast growth factor 21 (FGF21) is an endocrine-acting hormone that has the potential to treat diabetic nephropathy. However, development of FGF21 into a therapeutic has been hindered due to its low intrin...

    Authors: Longwei Zhao, Huiyan Wang, Junjun Xie, Zilu Chen, Xiaokun Li and Jianlou Niu

    Citation: BMC Biotechnology 2017 17:58

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  29. Content type: Research article

    Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performan...

    Authors: Xinzhe Wang, Huihua Ge, Dandan Zhang, Shuyu Wu and Guangya Zhang

    Citation: BMC Biotechnology 2017 17:57

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  30. Content type: Research article

    Fas ligand plays a key role in the human immune system as a major cell death inducing protein. The extracellular domain of human Fas ligand (hFasLECD) triggers apoptosis of malignant cells, and therefore is ex...

    Authors: Michiro Muraki and Kiyonori Hirota

    Citation: BMC Biotechnology 2017 17:56

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  31. Content type: Research Article

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Authors: Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    Citation: BMC Biotechnology 2017 17:55

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  32. Content type: Methodology article

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Authors: Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2017 17:54

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  33. Content type: Research article

    Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing ind...

    Authors: Hong Zhu, L. Bruce Reynolds and Rima Menassa

    Citation: BMC Biotechnology 2017 17:53

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  34. Content type: Research article

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Authors: Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    Citation: BMC Biotechnology 2017 17:52

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  35. Content type: Research article

    The discharge of poorly treated effluents into the environment has far reaching, consequential impacts on human and aquatic life forms. Thus, we evaluated the flocculating efficiency of our test bioflocculant ...

    Authors: Mayowa Oladele Agunbiade, Esta Van Heerden, Carolina H. Pohl and Anofi Tom Ashafa

    Citation: BMC Biotechnology 2017 17:51

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  36. Content type: Research article

    Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only...

    Authors: Yan-Jun Li, Shou-Hong Zhu, Xin-Yu Zhang, Yong-Chang Liu, Fei Xue, Lan-Jie Zhao and Jie Sun

    Citation: BMC Biotechnology 2017 17:50

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  37. Content type: Research article

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Authors: Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    Citation: BMC Biotechnology 2017 17:49

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  38. Content type: Research article

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by...

    Authors: Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: BMC Biotechnology 2017 17:48

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  42. Content type: Research article

    Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is pre...

    Authors: Martinus J.A. Daas, Patricia Murciano Martínez, Antonius H.P. van de Weijer, John van der Oost, Willem M. de Vos, Mirjam A. Kabel and Richard van Kranenburg

    Citation: BMC Biotechnology 2017 17:44

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  43. Content type: Methodology article

    Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these metho...

    Authors: Lisa A. Santry, Joelle C. Ingrao, Darrick L. Yu, Jondavid G. de Jong, Laura P. van Lieshout, Geoffrey A. Wood and Sarah K. Wootton

    Citation: BMC Biotechnology 2017 17:43

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  44. Content type: Research article

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Authors: Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    Citation: BMC Biotechnology 2017 17:42

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  45. Content type: Research Article

    At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated me...

    Authors: Darjan Andrejc, Alenka Možir and Matic Legiša

    Citation: BMC Biotechnology 2017 17:41

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  46. Content type: Research article

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Authors: Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    Citation: BMC Biotechnology 2017 17:40

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  48. Content type: Research article

    Propionate is widely used as an important preservative and important chemical intermediate for synthesis of cellulose fibers, herbicides, perfumes and pharmaceuticals. Biosynthetic propionate has mainly been p...

    Authors: Jing Li, Xinna Zhu, Jing Chen, Dongdong Zhao, Xueli Zhang and Changhao Bi

    Citation: BMC Biotechnology 2017 17:38

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  49. Content type: Research article

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

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  50. Content type: Research article

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Authors: Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    Citation: BMC Biotechnology 2017 17:36

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