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  1. Content type: Research article

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Authors: Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    Citation: BMC Biotechnology 2017 17:17

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  2. Content type: Research article

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Authors: Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    Citation: BMC Biotechnology 2017 17:15

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  3. Content type: Research article

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Authors: Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    Citation: BMC Biotechnology 2017 17:16

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  4. Content type: Research article

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Authors: Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    Citation: BMC Biotechnology 2017 17:14

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  5. Content type: Methodology article

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Authors: Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    Citation: BMC Biotechnology 2017 17:13

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  6. Content type: Research article

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Authors: Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    Citation: BMC Biotechnology 2017 17:12

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  7. Content type: Research article

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Authors: Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    Citation: BMC Biotechnology 2017 17:11

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  8. Content type: Research article

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Authors: Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    Citation: BMC Biotechnology 2017 17:9

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  9. Content type: Research article

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Authors: Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    Citation: BMC Biotechnology 2017 17:8

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  10. Content type: Research article

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Authors: Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    Citation: BMC Biotechnology 2017 17:7

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  11. Content type: Research article

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Authors: Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    Citation: BMC Biotechnology 2017 17:6

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  12. Content type: Research article

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    Authors: D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    Citation: BMC Biotechnology 2017 17:4

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  13. Content type: Methodology article

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Authors: Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    Citation: BMC Biotechnology 2017 17:3

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  14. Content type: Research article

    Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus wa...

    Authors: Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang and Hui-Wen Chen

    Citation: BMC Biotechnology 2017 17:2

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  15. Content type: Methodology article

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Authors: Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    Citation: BMC Biotechnology 2017 17:1

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  16. Content type: Research article

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Authors: Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    Citation: BMC Biotechnology 2016 16:89

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  17. Content type: Methodology article

    Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenes...

    Authors: Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro, Renaud Vincentelli and Carlos M. G. A. Fontes

    Citation: BMC Biotechnology 2016 16:86

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  18. Content type: Research article

    Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escheri...

    Authors: Nan Wang, Kai Ren, Rong Jia, Wenting Chen and Ruirui Sun

    Citation: BMC Biotechnology 2016 16:87

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  19. Content type: Research article

    Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is ...

    Authors: Yahaira Naaldijk, Adiv A. Johnson, Annett Friedrich-Stöckigt and Alexandra Stolzing

    Citation: BMC Biotechnology 2016 16:85

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  20. Content type: Research article

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Authors: Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    Citation: BMC Biotechnology 2016 16:84

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  21. Content type: Methodology article

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Authors: Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    Citation: BMC Biotechnology 2016 16:83

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  22. Content type: Research article

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Authors: Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    Citation: BMC Biotechnology 2016 16:81

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  23. Content type: Research article

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Authors: Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    Citation: BMC Biotechnology 2016 16:82

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  24. Content type: Research article

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    Authors: André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    Citation: BMC Biotechnology 2016 16:80

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  25. Content type: Research article

    Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Authors: Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    Citation: BMC Biotechnology 2016 16:79

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  26. Content type: Research article

    The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Authors: Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    Citation: BMC Biotechnology 2016 16:78

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  27. Content type: Methodology article

    Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Authors: Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    Citation: BMC Biotechnology 2016 16:76

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  28. Content type: Research article

    Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of family 11 alkaline xylanase Xyn11A-LC from Bacillus s...

    Authors: Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang and Zhenhu Jia

    Citation: BMC Biotechnology 2016 16:77

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  29. Content type: Research article

    Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptom...

    Authors: Song Liu, Miao Wang, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2016 16:75

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  30. Content type: Research article

    The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...

    Authors: Hongwei Gao, Xiaofan Yu, Tingting Deng, Min Sun, Xizhi Xiao, Xin Huang, Ying Chen and Ronggui Li

    Citation: BMC Biotechnology 2016 16:74

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  31. Content type: Research article

    Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended ...

    Authors: Shiping Liu and Shaojun Ding

    Citation: BMC Biotechnology 2016 16:73

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  32. Content type: Research article

    A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of ...

    Authors: Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng and Chang-Guo Zhan

    Citation: BMC Biotechnology 2016 16:72

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  33. Content type: Research article

    Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter d...

    Authors: Steven C. L. Ho, Esther Y. C. Koh, Benjamin P. C. Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang

    Citation: BMC Biotechnology 2016 16:71

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  34. Content type: Research article

    Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (CO...

    Authors: Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin and Yu-Guo Zheng

    Citation: BMC Biotechnology 2016 16:70

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  35. Content type: Research article

    Abiotic stresses are serious threats to plant growth, productivity and result in crop loss worldwide, reducing average yields of most major crops. Although abiotic stresses might elicit different plant respons...

    Authors: Yuanyuan Bu, Bo Sun, Aimin Zhou, Xinxin Zhang, Testuo Takano and Shenkui Liu

    Citation: BMC Biotechnology 2016 16:69

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  36. Content type: Research article

    RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five treh...

    Authors: Bin Tang, Ping Wei, Lina Zhao, Zuokun Shi, Qida Shen, Mengmeng Yang, Guoqiang Xie and Shigui Wang

    Citation: BMC Biotechnology 2016 16:67

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  37. Content type: Methodology article

    Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is ...

    Authors: Alexander Falkenhagen, Sabah Asad, Stanley E. Read and Sadhna Joshi

    Citation: BMC Biotechnology 2016 16:66

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  38. Content type: Research article

    Targeted therapies for cancer, especially the malignant cancer, are always restricted by the deficiency of tumor-specific drug delivery methods. Subtilase cytotoxic is a virulent cytotoxin, and the subunit A (...

    Authors: Lichao Zhang, Zongwei Li, Tonglin Shi, Xiaoqin La, Hanqing Li and Zhuoyu Li

    Citation: BMC Biotechnology 2016 16:65

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  39. Content type: Methodology article

    Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesvi...

    Authors: Alexsia L. Richards, Patricia J. Sollars and Gregory A. Smith

    Citation: BMC Biotechnology 2016 16:64

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  40. Content type: Research article

    Transcription factors (TFs) are effectors of cell signaling pathways that regulate gene expression. TF networks are highly interconnected; one signal can lead to changes in many TF levels, and one TF level can...

    Authors: Betul Bilgin, Aritro Nath, Christina Chan and S. Patrick Walton

    Citation: BMC Biotechnology 2016 16:62

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  41. Content type: Research article

    Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer ...

    Authors: Yanning Zheng, Lingling Li, Qiang Liu, Haibo Zhang, Yujin Cao, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:61

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  42. Content type: Methodology article

    Selected microRNAs (miRNAs) that are abnormally expressed in the serum of patients with lung cancer have recently been proposed as biomarkers of this disease. The measurement of circulating miRNAs, however, re...

    Authors: Paola Campomenosi, Elisabetta Gini, Douglas M. Noonan, Albino Poli, Paola D’Antona, Nicola Rotolo, Lorenzo Dominioni and Andrea Imperatori

    Citation: BMC Biotechnology 2016 16:60

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  43. Content type: Methodology article

    CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice.

    Authors: Weiwei Qi, Tong Zhu, Zhongrui Tian, Chaobin Li, Wei Zhang and Rentao Song

    Citation: BMC Biotechnology 2016 16:58

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  44. Content type: Methodology article

    Large efforts have focused on screening for genes involved in the virulence and pathogenicity of Verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the...

    Authors: Latifur Rehman, Xiaofeng Su, Huiming Guo, Xiliang Qi and Hongmei Cheng

    Citation: BMC Biotechnology 2016 16:57

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