Fig. 6
The 25 mm SS device (0.1 mL membrane volume) (a), the NatriFlo® HD-Q Recon Mini (0.2 mL membrane volume) (b) and the NatriFlo® HD-Q Recon (0.8 mL membrane volume) (c) were loaded with allantoic fluid containing NDV (VL) followed by two wash steps (W) and an elution step (E) and binding kinetics evaluated with increasing membrane volumes. The amount of infectious virus and protein in the eluate was quantified over time by TCID50 assay and Bradford protein assay, respectively. Runs were repeated three times. Proteins were analyzed in triplicate, virus samples (n = 3) were pooled and titered. Means ±SD are shown