Fig. 2

Viability of cells seeded through stencil. a Fluorescent micrograph of HME1 cell nuclei (blue), live cells (green), and dead/dying cells (red) seeded onto a microwell array substrate with and without a stencil. Cells were imaged after cell adhesion and removal of the stencil film. Arrows indicate equivalent positions in each color channel. b Fluorescent micrograph of MDA-MB-231 cell nuclei (blue), live cells (green), and dead/dying cells (red) seeded onto a microwell array substrate with (top) and without (bottom) a stencil. Arrows indicate equivalent positions in each color channel. c Cell viability of HME and MDA-MB-231 cells seeded with and without stencil. Data pooled from 12 independent experiments. Error bars indicate the SEM