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Fig. 2 | BMC Biotechnology

Fig. 2

From: Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria

Fig. 2

Nine toolkit promoters drive nifU expression in different carbon sources. a Anti-NifU WB analysis of inducible nifU expression driven by the GAL1p in exponentially growing cultures containing glucose or galactose as carbon source (CS) as indicated (constructs inserted into YORWΔ22 locus of strain W303). The lower panel shows the corresponding membrane Coomassie stained as a loading control. b-c Anti-NifU WB analysis of nifU expression driven by different promoters (constructs inserted into YPRCΔ15 locus of strain W303) in exponentially growing cultures containing glucose (b) or glycerol (c) as the carbon source. The lower panel shows the corresponding membrane Coomassie stained as a loading control

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BMC Biotechnology

ISSN: 1472-6750

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