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Table 4 Advantages and disadvantages of the tested techniques

From: Overview of methodologies for T-cell receptor repertoire analysis

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BGI • Deep sequencing: more complete data
• Raw data and analysis provided by the company
• Company service: no hands-on work
• gDNA as starting material: better clonotype quantification
• Expensive compared to in-house methods
• Multiplex PCR amplification bias
• Limited PCR and sequencing errors correction
• gDNA as starting material: not final TCR product
5’RACE • In-house method: control of all steps, relatively cheap
• No multiplex PCR bias
• Unique Molecular Identifiers: correction for PCR and sequencing errors
• Superficial sequencing: less diversity detected
• Not high-throughput: small sample number processed per time
iRepertoire® • Kit: easy and fast hands-on (less than one day)
• De-multiplexing and basic data analysis made by the company. FASTA files provided
• Multiplex PCR amplification bias
• Limited PCR and sequencing errors correction