Overview of methodologies for T-cell receptor repertoire analysis

BMC Biotechnology

Table 2 Percentages of CDR3 nucleotide sequences detected in both duplicates of the same method

Replicates shared clonotypes percentages	α chain			β chain
Replicates shared clonotypes percentages	iRepertoire	5’RACE	5’RACE + UMI	iRepertoire	5’RACE	5’RACE + UMI
All clonotypes	36	44	20	35	52	25
Top 300 clonotypes	31	26	27	35	32	37
Top 100 clonotypes	37	31	36	46	51	51
Top 50 clonotypes	45	44	50	38	64	64
Top 20 clonotypes	60	65	70	50	80	75

The percentages are shown for comparisons made between all of the observed clonotypes and between the 300, 100, 50 and 20 most abundant sequences detected by each method. Results include iRepertoire kit data, 5’RACE-based PCR data and data from the same PCR corrected using unique molecular identifiers. Data are shown for both α and β chains

ISSN: 1472-6750