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Table 2 Percentages of CDR3 nucleotide sequences detected in both duplicates of the same method

From: Overview of methodologies for T-cell receptor repertoire analysis

Replicates shared
clonotypes percentages
α chain β chain
iRepertoire 5’RACE 5’RACE + UMI iRepertoire 5’RACE 5’RACE + UMI
All clonotypes 36 44 20 35 52 25
Top 300 clonotypes 31 26 27 35 32 37
Top 100 clonotypes 37 31 36 46 51 51
Top 50 clonotypes 45 44 50 38 64 64
Top 20 clonotypes 60 65 70 50 80 75
  1. The percentages are shown for comparisons made between all of the observed clonotypes and between the 300, 100, 50 and 20 most abundant sequences detected by each method. Results include iRepertoire kit data, 5’RACE-based PCR data and data from the same PCR corrected using unique molecular identifiers. Data are shown for both α and β chains