Fig. 6

The PCR and qPCR detection of transgenic plants. a, Schematic representation of the expression cassettes in the plant expression vector pGWB17-GhKIS13A1 used in Arabidopsis transformation. 35S pm, CaMV 35S promoter; NOS tm, NOS terminor; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase IIgene; attB1 and attB2, recombination site sequences in the Gateway system; GhKIS13A1, cDNA sequence of the GhKIS13A1 gene; RB, right border; LB, left border; (b), The expression level of GhKIS13A1 in wild-type (Col-0) and three GhKIS13A1-OX lines (L1, L2 and L8). WT, wild-type; (c), The expression level of GhKIS13A1 in the Kinesin-13a-1 mutant and three GhKIS13A1-COM lines (C3, C7 and C10). M, Kinesin-13a-1 mutant. All expression levels are relative to the Arabidopsis ACTIN2 gene that was used as an internal control