Fig. 5

Gross images of alkaline phosphatase staining in the upper and lower respiratory tract of mice following intratracheal administration of an AAV vector expressing hPLAP. AAV vector (1x10¹¹ vg) was administered by intratracheal injection to 7-week-old C57BL/6 mice and tissues evaluated for hPLAP expression after three weeks. Intratracheal injection resulted in intense hPLAP staining in the trachea with some staining in the nasal epithelium. This delivery method resulted in variable hPLAP expression in the lower respiratory tract, such that the proximal and mid-distal aspect of the lower respiratory tract were well transduced in three (a, b and c) of four mice and in two of the four mice, hPLAP staining was observed in the distal aspect of some of the lung lobes, mainly the largest lung lobe (a and b). Letters a-d represent individual mice and numbers one through five indicate the accessory lobe (1), the left lobe (2), the right cranial lobe (3), the right caudal lobe (4) and the right middle lobe (5)