Fig. 3

Gross images of alkaline phosphatase staining in the upper and lower respiratory tract of mice following administration of an AAV vector expressing hPLAP using the modified intranasal technique. AAV vector (1x10¹¹ vg) was administered by the modified intranasal method to 7-week-old C57BL/6 mice and tissues evaluated for hPLAP expression after three weeks. The modified intranasal technique resulted in intense staining of the upper respiratory tract, including nasal epithelium and trachea, as well as the proximal and mid-distal aspect of the lower respiratory tract in all mice examined. Letters a-d represent individual mice and numbers one through five indicate the accessory lobe (1), the left lobe (2), the right cranial lobe (3), the right caudal lobe (4) and the right middle lobe (5)